Amino acid dipepetide frequency for Pseudomonas phage POR1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.01AlaAla: 13.01 ± 1.239
0.983AlaCys: 0.983 ± 0.274
5.955AlaAsp: 5.955 ± 0.612
6.996AlaGlu: 6.996 ± 0.808
3.758AlaPhe: 3.758 ± 0.394
7.806AlaGly: 7.806 ± 0.902
2.082AlaHis: 2.082 ± 0.445
5.609AlaIle: 5.609 ± 0.599
5.146AlaLys: 5.146 ± 0.622
8.153AlaLeu: 8.153 ± 0.753
2.891AlaMet: 2.891 ± 0.341
3.7AlaAsn: 3.7 ± 0.51
5.898AlaPro: 5.898 ± 0.869
5.377AlaGln: 5.377 ± 0.537
5.955AlaArg: 5.955 ± 0.629
6.129AlaSer: 6.129 ± 0.639
6.418AlaThr: 6.418 ± 0.573
8.21AlaVal: 8.21 ± 0.813
1.619AlaTrp: 1.619 ± 0.264
3.238AlaTyr: 3.238 ± 0.452
0.0AlaXaa: 0.0 ± 0.0
Cys
0.867CysAla: 0.867 ± 0.2
0.231CysCys: 0.231 ± 0.093
0.752CysAsp: 0.752 ± 0.227
0.694CysGlu: 0.694 ± 0.246
0.231CysPhe: 0.231 ± 0.13
0.983CysGly: 0.983 ± 0.264
0.289CysHis: 0.289 ± 0.139
0.578CysIle: 0.578 ± 0.158
0.347CysLys: 0.347 ± 0.136
0.809CysLeu: 0.809 ± 0.254
0.347CysMet: 0.347 ± 0.156
0.463CysAsn: 0.463 ± 0.177
0.578CysPro: 0.578 ± 0.198
0.405CysGln: 0.405 ± 0.147
0.809CysArg: 0.809 ± 0.228
0.809CysSer: 0.809 ± 0.202
0.289CysThr: 0.289 ± 0.122
0.694CysVal: 0.694 ± 0.22
0.116CysTrp: 0.116 ± 0.085
0.347CysTyr: 0.347 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
6.823AspAla: 6.823 ± 0.595
0.809AspCys: 0.809 ± 0.219
2.833AspAsp: 2.833 ± 0.516
3.238AspGlu: 3.238 ± 0.456
2.082AspPhe: 2.082 ± 0.365
4.799AspGly: 4.799 ± 0.489
1.503AspHis: 1.503 ± 0.376
2.139AspIle: 2.139 ± 0.32
3.296AspLys: 3.296 ± 0.473
4.221AspLeu: 4.221 ± 0.545
1.33AspMet: 1.33 ± 0.261
2.371AspAsn: 2.371 ± 0.426
2.602AspPro: 2.602 ± 0.434
1.446AspGln: 1.446 ± 0.318
2.428AspArg: 2.428 ± 0.338
3.354AspSer: 3.354 ± 0.497
4.105AspThr: 4.105 ± 0.437
3.758AspVal: 3.758 ± 0.415
0.636AspTrp: 0.636 ± 0.16
2.255AspTyr: 2.255 ± 0.357
0.0AspXaa: 0.0 ± 0.0
Glu
7.517GluAla: 7.517 ± 0.632
0.809GluCys: 0.809 ± 0.196
2.775GluAsp: 2.775 ± 0.417
2.602GluGlu: 2.602 ± 0.402
2.313GluPhe: 2.313 ± 0.367
3.7GluGly: 3.7 ± 0.465
1.677GluHis: 1.677 ± 0.383
2.718GluIle: 2.718 ± 0.433
2.949GluLys: 2.949 ± 0.355
6.129GluLeu: 6.129 ± 0.712
1.908GluMet: 1.908 ± 0.384
3.18GluAsn: 3.18 ± 0.386
2.197GluPro: 2.197 ± 0.366
2.775GluGln: 2.775 ± 0.375
5.204GluArg: 5.204 ± 0.584
2.833GluSer: 2.833 ± 0.5
3.122GluThr: 3.122 ± 0.439
3.238GluVal: 3.238 ± 0.476
0.983GluTrp: 0.983 ± 0.207
1.735GluTyr: 1.735 ± 0.318
0.0GluXaa: 0.0 ± 0.0
Phe
3.816PheAla: 3.816 ± 0.462
0.289PheCys: 0.289 ± 0.126
2.775PheAsp: 2.775 ± 0.46
2.139PheGlu: 2.139 ± 0.383
1.446PhePhe: 1.446 ± 0.285
2.313PheGly: 2.313 ± 0.297
0.578PheHis: 0.578 ± 0.153
1.561PheIle: 1.561 ± 0.302
1.619PheLys: 1.619 ± 0.383
2.833PheLeu: 2.833 ± 0.466
0.694PheMet: 0.694 ± 0.205
1.792PheAsn: 1.792 ± 0.314
1.214PhePro: 1.214 ± 0.259
1.272PheGln: 1.272 ± 0.288
2.313PheArg: 2.313 ± 0.43
2.313PheSer: 2.313 ± 0.455
1.503PheThr: 1.503 ± 0.331
3.238PheVal: 3.238 ± 0.442
0.52PheTrp: 0.52 ± 0.213
1.792PheTyr: 1.792 ± 0.306
0.0PheXaa: 0.0 ± 0.0
Gly
8.789GlyAla: 8.789 ± 0.959
0.809GlyCys: 0.809 ± 0.259
5.03GlyAsp: 5.03 ± 0.926
4.626GlyGlu: 4.626 ± 0.612
2.775GlyPhe: 2.775 ± 0.352
5.898GlyGly: 5.898 ± 0.625
1.561GlyHis: 1.561 ± 0.324
4.279GlyIle: 4.279 ± 0.437
4.394GlyLys: 4.394 ± 0.491
4.973GlyLeu: 4.973 ± 0.474
2.255GlyMet: 2.255 ± 0.381
2.602GlyAsn: 2.602 ± 0.55
2.371GlyPro: 2.371 ± 0.356
3.816GlyGln: 3.816 ± 0.931
4.741GlyArg: 4.741 ± 0.62
4.626GlySer: 4.626 ± 0.628
4.568GlyThr: 4.568 ± 0.884
6.649GlyVal: 6.649 ± 0.669
1.33GlyTrp: 1.33 ± 0.299
3.064GlyTyr: 3.064 ± 0.471
0.0GlyXaa: 0.0 ± 0.0
His
2.371HisAla: 2.371 ± 0.388
0.405HisCys: 0.405 ± 0.153
1.33HisAsp: 1.33 ± 0.323
1.561HisGlu: 1.561 ± 0.363
0.809HisPhe: 0.809 ± 0.208
1.214HisGly: 1.214 ± 0.281
0.347HisHis: 0.347 ± 0.137
0.809HisIle: 0.809 ± 0.164
0.983HisLys: 0.983 ± 0.257
1.503HisLeu: 1.503 ± 0.322
0.867HisMet: 0.867 ± 0.215
0.867HisAsn: 0.867 ± 0.282
0.867HisPro: 0.867 ± 0.193
0.405HisGln: 0.405 ± 0.168
1.041HisArg: 1.041 ± 0.203
1.33HisSer: 1.33 ± 0.582
1.446HisThr: 1.446 ± 0.416
1.735HisVal: 1.735 ± 0.398
0.347HisTrp: 0.347 ± 0.147
0.752HisTyr: 0.752 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
4.105IleAla: 4.105 ± 0.439
0.347IleCys: 0.347 ± 0.158
2.891IleAsp: 2.891 ± 0.453
3.99IleGlu: 3.99 ± 0.408
0.983IlePhe: 0.983 ± 0.232
3.122IleGly: 3.122 ± 0.506
0.867IleHis: 0.867 ± 0.207
2.082IleIle: 2.082 ± 0.369
2.428IleLys: 2.428 ± 0.335
2.833IleLeu: 2.833 ± 0.463
0.694IleMet: 0.694 ± 0.163
2.313IleAsn: 2.313 ± 0.423
2.544IlePro: 2.544 ± 0.306
2.197IleGln: 2.197 ± 0.31
3.469IleArg: 3.469 ± 0.518
2.949IleSer: 2.949 ± 0.42
2.949IleThr: 2.949 ± 0.405
3.122IleVal: 3.122 ± 0.421
0.347IleTrp: 0.347 ± 0.142
1.156IleTyr: 1.156 ± 0.253
0.0IleXaa: 0.0 ± 0.0
Lys
5.724LysAla: 5.724 ± 0.591
0.578LysCys: 0.578 ± 0.186
2.371LysAsp: 2.371 ± 0.371
3.296LysGlu: 3.296 ± 0.442
1.388LysPhe: 1.388 ± 0.258
4.683LysGly: 4.683 ± 0.81
1.099LysHis: 1.099 ± 0.255
2.371LysIle: 2.371 ± 0.289
1.966LysLys: 1.966 ± 0.357
4.626LysLeu: 4.626 ± 0.472
1.446LysMet: 1.446 ± 0.283
2.082LysAsn: 2.082 ± 0.366
2.428LysPro: 2.428 ± 0.453
1.792LysGln: 1.792 ± 0.302
3.469LysArg: 3.469 ± 0.444
2.891LysSer: 2.891 ± 0.455
2.197LysThr: 2.197 ± 0.299
3.932LysVal: 3.932 ± 0.481
0.694LysTrp: 0.694 ± 0.162
1.503LysTyr: 1.503 ± 0.304
0.0LysXaa: 0.0 ± 0.0
Leu
8.557LeuAla: 8.557 ± 0.876
0.694LeuCys: 0.694 ± 0.184
4.105LeuAsp: 4.105 ± 0.536
5.03LeuGlu: 5.03 ± 0.444
2.486LeuPhe: 2.486 ± 0.358
4.683LeuGly: 4.683 ± 0.545
1.388LeuHis: 1.388 ± 0.303
3.816LeuIle: 3.816 ± 0.549
4.394LeuLys: 4.394 ± 0.473
6.707LeuLeu: 6.707 ± 0.838
1.85LeuMet: 1.85 ± 0.326
4.337LeuAsn: 4.337 ± 0.458
4.047LeuPro: 4.047 ± 0.599
3.354LeuGln: 3.354 ± 0.409
5.898LeuArg: 5.898 ± 0.592
4.683LeuSer: 4.683 ± 0.474
5.262LeuThr: 5.262 ± 0.613
3.816LeuVal: 3.816 ± 0.543
1.099LeuTrp: 1.099 ± 0.245
2.082LeuTyr: 2.082 ± 0.375
0.0LeuXaa: 0.0 ± 0.0
Met
3.354MetAla: 3.354 ± 0.436
0.347MetCys: 0.347 ± 0.166
1.099MetAsp: 1.099 ± 0.307
1.156MetGlu: 1.156 ± 0.238
0.752MetPhe: 0.752 ± 0.236
1.561MetGly: 1.561 ± 0.238
0.694MetHis: 0.694 ± 0.181
0.867MetIle: 0.867 ± 0.184
1.561MetLys: 1.561 ± 0.275
1.619MetLeu: 1.619 ± 0.307
0.578MetMet: 0.578 ± 0.204
1.792MetAsn: 1.792 ± 0.354
1.735MetPro: 1.735 ± 0.485
1.966MetGln: 1.966 ± 0.495
2.024MetArg: 2.024 ± 0.322
1.735MetSer: 1.735 ± 0.295
1.446MetThr: 1.446 ± 0.286
1.099MetVal: 1.099 ± 0.291
0.289MetTrp: 0.289 ± 0.134
0.809MetTyr: 0.809 ± 0.19
0.0MetXaa: 0.0 ± 0.0
Asn
5.03AsnAla: 5.03 ± 0.603
0.405AsnCys: 0.405 ± 0.151
3.585AsnAsp: 3.585 ± 0.532
2.082AsnGlu: 2.082 ± 0.411
1.792AsnPhe: 1.792 ± 0.27
5.088AsnGly: 5.088 ± 0.613
0.578AsnHis: 0.578 ± 0.198
1.792AsnIle: 1.792 ± 0.329
2.082AsnLys: 2.082 ± 0.327
3.469AsnLeu: 3.469 ± 0.458
1.156AsnMet: 1.156 ± 0.286
1.561AsnAsn: 1.561 ± 0.325
2.313AsnPro: 2.313 ± 0.344
1.619AsnGln: 1.619 ± 0.325
2.139AsnArg: 2.139 ± 0.319
2.66AsnSer: 2.66 ± 0.402
2.544AsnThr: 2.544 ± 0.418
3.296AsnVal: 3.296 ± 0.452
0.809AsnTrp: 0.809 ± 0.205
1.156AsnTyr: 1.156 ± 0.268
0.0AsnXaa: 0.0 ± 0.0
Pro
4.857ProAla: 4.857 ± 0.532
0.173ProCys: 0.173 ± 0.095
2.313ProAsp: 2.313 ± 0.364
3.18ProGlu: 3.18 ± 0.465
2.255ProPhe: 2.255 ± 0.363
5.03ProGly: 5.03 ± 0.867
0.809ProHis: 0.809 ± 0.219
1.214ProIle: 1.214 ± 0.26
2.197ProLys: 2.197 ± 0.327
3.122ProLeu: 3.122 ± 0.377
1.677ProMet: 1.677 ± 0.435
2.775ProAsn: 2.775 ± 0.427
2.255ProPro: 2.255 ± 0.614
2.66ProGln: 2.66 ± 1.446
2.428ProArg: 2.428 ± 0.369
2.891ProSer: 2.891 ± 0.467
2.718ProThr: 2.718 ± 0.582
2.949ProVal: 2.949 ± 0.498
0.578ProTrp: 0.578 ± 0.188
1.156ProTyr: 1.156 ± 0.312
0.0ProXaa: 0.0 ± 0.0
Gln
4.799GlnAla: 4.799 ± 0.631
0.463GlnCys: 0.463 ± 0.175
2.082GlnAsp: 2.082 ± 0.375
2.313GlnGlu: 2.313 ± 0.358
1.388GlnPhe: 1.388 ± 0.278
3.411GlnGly: 3.411 ± 0.83
1.214GlnHis: 1.214 ± 0.232
1.85GlnIle: 1.85 ± 0.27
2.024GlnLys: 2.024 ± 0.292
3.122GlnLeu: 3.122 ± 0.34
1.85GlnMet: 1.85 ± 0.442
1.85GlnAsn: 1.85 ± 0.303
2.139GlnPro: 2.139 ± 0.972
4.452GlnGln: 4.452 ± 2.033
3.007GlnArg: 3.007 ± 0.453
2.082GlnSer: 2.082 ± 0.313
2.602GlnThr: 2.602 ± 0.32
1.85GlnVal: 1.85 ± 0.322
0.694GlnTrp: 0.694 ± 0.192
1.446GlnTyr: 1.446 ± 0.366
0.0GlnXaa: 0.0 ± 0.0
Arg
6.534ArgAla: 6.534 ± 0.716
0.867ArgCys: 0.867 ± 0.233
2.718ArgAsp: 2.718 ± 0.486
4.394ArgGlu: 4.394 ± 0.618
2.602ArgPhe: 2.602 ± 0.338
3.469ArgGly: 3.469 ± 0.46
1.156ArgHis: 1.156 ± 0.352
2.833ArgIle: 2.833 ± 0.364
3.411ArgLys: 3.411 ± 0.409
6.013ArgLeu: 6.013 ± 0.678
2.139ArgMet: 2.139 ± 0.315
2.718ArgAsn: 2.718 ± 0.377
1.85ArgPro: 1.85 ± 0.29
2.486ArgGln: 2.486 ± 0.34
3.874ArgArg: 3.874 ± 0.617
3.18ArgSer: 3.18 ± 0.404
2.775ArgThr: 2.775 ± 0.396
5.204ArgVal: 5.204 ± 0.546
0.867ArgTrp: 0.867 ± 0.205
2.255ArgTyr: 2.255 ± 0.386
0.0ArgXaa: 0.0 ± 0.0
Ser
6.649SerAla: 6.649 ± 0.681
0.694SerCys: 0.694 ± 0.239
3.643SerAsp: 3.643 ± 0.483
3.354SerGlu: 3.354 ± 0.537
1.735SerPhe: 1.735 ± 0.355
4.973SerGly: 4.973 ± 0.596
1.33SerHis: 1.33 ± 0.376
2.891SerIle: 2.891 ± 0.329
3.064SerLys: 3.064 ± 0.42
4.683SerLeu: 4.683 ± 0.394
0.925SerMet: 0.925 ± 0.305
2.428SerAsn: 2.428 ± 0.404
2.082SerPro: 2.082 ± 0.651
2.371SerGln: 2.371 ± 0.308
3.238SerArg: 3.238 ± 0.538
3.585SerSer: 3.585 ± 0.502
3.411SerThr: 3.411 ± 0.499
4.568SerVal: 4.568 ± 0.451
0.578SerTrp: 0.578 ± 0.182
1.214SerTyr: 1.214 ± 0.232
0.0SerXaa: 0.0 ± 0.0
Thr
6.129ThrAla: 6.129 ± 0.805
0.636ThrCys: 0.636 ± 0.205
3.643ThrAsp: 3.643 ± 0.425
3.122ThrGlu: 3.122 ± 0.426
2.544ThrPhe: 2.544 ± 0.352
6.129ThrGly: 6.129 ± 0.542
1.388ThrHis: 1.388 ± 0.408
3.238ThrIle: 3.238 ± 0.504
2.313ThrLys: 2.313 ± 0.333
4.799ThrLeu: 4.799 ± 0.553
1.041ThrMet: 1.041 ± 0.234
2.775ThrAsn: 2.775 ± 0.546
3.585ThrPro: 3.585 ± 0.552
1.561ThrGln: 1.561 ± 0.311
2.66ThrArg: 2.66 ± 0.353
3.18ThrSer: 3.18 ± 0.46
3.527ThrThr: 3.527 ± 0.598
3.932ThrVal: 3.932 ± 0.548
0.752ThrTrp: 0.752 ± 0.188
2.371ThrTyr: 2.371 ± 0.336
0.0ThrXaa: 0.0 ± 0.0
Val
5.493ValAla: 5.493 ± 0.506
0.694ValCys: 0.694 ± 0.255
3.411ValAsp: 3.411 ± 0.514
4.279ValGlu: 4.279 ± 0.517
2.66ValPhe: 2.66 ± 0.336
6.187ValGly: 6.187 ± 0.692
1.214ValHis: 1.214 ± 0.24
3.064ValIle: 3.064 ± 0.383
4.452ValLys: 4.452 ± 0.549
4.915ValLeu: 4.915 ± 0.541
1.619ValMet: 1.619 ± 0.313
3.527ValAsn: 3.527 ± 0.475
3.874ValPro: 3.874 ± 0.566
2.486ValGln: 2.486 ± 0.336
3.758ValArg: 3.758 ± 0.431
4.221ValSer: 4.221 ± 0.495
5.319ValThr: 5.319 ± 0.752
2.949ValVal: 2.949 ± 0.393
0.867ValTrp: 0.867 ± 0.19
1.677ValTyr: 1.677 ± 0.403
0.0ValXaa: 0.0 ± 0.0
Trp
1.214TrpAla: 1.214 ± 0.228
0.231TrpCys: 0.231 ± 0.111
0.694TrpAsp: 0.694 ± 0.195
0.52TrpGlu: 0.52 ± 0.179
0.809TrpPhe: 0.809 ± 0.222
0.752TrpGly: 0.752 ± 0.207
0.578TrpHis: 0.578 ± 0.196
0.578TrpIle: 0.578 ± 0.215
0.463TrpLys: 0.463 ± 0.148
1.735TrpLeu: 1.735 ± 0.305
0.405TrpMet: 0.405 ± 0.141
0.52TrpAsn: 0.52 ± 0.159
1.041TrpPro: 1.041 ± 0.278
0.809TrpGln: 0.809 ± 0.172
0.867TrpArg: 0.867 ± 0.257
0.347TrpSer: 0.347 ± 0.137
1.041TrpThr: 1.041 ± 0.226
0.636TrpVal: 0.636 ± 0.207
0.173TrpTrp: 0.173 ± 0.107
0.52TrpTyr: 0.52 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.296TyrAla: 3.296 ± 0.424
0.173TyrCys: 0.173 ± 0.131
2.024TyrAsp: 2.024 ± 0.392
1.792TyrGlu: 1.792 ± 0.343
1.214TyrPhe: 1.214 ± 0.283
3.354TyrGly: 3.354 ± 0.466
0.694TyrHis: 0.694 ± 0.209
1.099TyrIle: 1.099 ± 0.283
1.446TyrLys: 1.446 ± 0.355
2.024TyrLeu: 2.024 ± 0.287
0.752TyrMet: 0.752 ± 0.243
1.503TyrAsn: 1.503 ± 0.237
1.619TyrPro: 1.619 ± 0.291
1.388TyrGln: 1.388 ± 0.252
2.024TyrArg: 2.024 ± 0.359
1.619TyrSer: 1.619 ± 0.323
2.082TyrThr: 2.082 ± 0.374
1.792TyrVal: 1.792 ± 0.368
0.636TyrTrp: 0.636 ± 0.203
0.925TyrTyr: 0.925 ± 0.224
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (17296 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski