Sphingopyxis lindanitolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingopyxis

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4026 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S8B8U7|A0A2S8B8U7_9SPHN Acyl-CoA dehydrogenase OS=Sphingopyxis lindanitolerans OX=2054227 GN=CVO77_09895 PE=3 SV=1
MM1 pKa = 7.3AQLDD5 pKa = 3.86VFRR8 pKa = 11.84TDD10 pKa = 2.76EE11 pKa = 4.5GEE13 pKa = 4.11YY14 pKa = 11.18LLDD17 pKa = 4.11FQSDD21 pKa = 3.79TLDD24 pKa = 3.47HH25 pKa = 6.43YY26 pKa = 9.64NTRR29 pKa = 11.84FVIPLVSPDD38 pKa = 4.35HH39 pKa = 6.11GPKK42 pKa = 10.08LAEE45 pKa = 3.91RR46 pKa = 11.84LNPVFAIDD54 pKa = 4.35DD55 pKa = 4.11EE56 pKa = 4.54PMALYY61 pKa = 8.83TQFALTVPVADD72 pKa = 4.25LKK74 pKa = 11.54YY75 pKa = 10.27FVTSLSDD82 pKa = 2.84QHH84 pKa = 7.33YY85 pKa = 10.34LVMSALDD92 pKa = 3.79MLISGYY98 pKa = 10.95

Molecular weight: 11.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S8B8K8|A0A2S8B8K8_9SPHN Tryptophan--tRNA ligase OS=Sphingopyxis lindanitolerans OX=2054227 GN=trpS PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.57GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.68ATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLANRR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.26KK41 pKa = 10.65LSAA44 pKa = 3.91

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4026

0

4026

1288095

29

3212

319.9

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.809 ± 0.06

0.791 ± 0.011

6.225 ± 0.03

5.205 ± 0.036

3.578 ± 0.026

9.057 ± 0.049

2.012 ± 0.02

5.189 ± 0.027

2.997 ± 0.033

9.7 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.02

2.483 ± 0.026

5.341 ± 0.034

2.948 ± 0.02

7.327 ± 0.039

5.16 ± 0.03

5.117 ± 0.031

6.919 ± 0.034

1.469 ± 0.016

2.24 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski