Streptococcus phage CHPC930
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8FCH9|A0A3G8FCH9_9CAUD Holliday junction resolvase OS=Streptococcus phage CHPC930 OX=2365051 GN=CHPC930_0036 PE=4 SV=1
MM1 pKa = 6.82 ITKK4 pKa = 9.33 EE5 pKa = 3.87 QLKK8 pKa = 9.98 EE9 pKa = 3.95 YY10 pKa = 10.04 YY11 pKa = 9.76 SEE13 pKa = 4.08 HH14 pKa = 8.22 LEE16 pKa = 4.53 EE17 pKa = 4.21 LVEE20 pKa = 4.04 WADD23 pKa = 5.14 DD24 pKa = 3.53 INKK27 pKa = 9.26 ICLFAYY33 pKa = 10.47 LDD35 pKa = 4.23 EE36 pKa = 6.39 DD37 pKa = 4.14 DD38 pKa = 5.96 SLYY41 pKa = 11.19 CGINQLSYY49 pKa = 8.92 TQFRR53 pKa = 11.84 VPIQAEE59 pKa = 4.28 VTVDD63 pKa = 4.08 DD64 pKa = 4.11 DD65 pKa = 3.64 WNYY68 pKa = 11.7 DD69 pKa = 3.57 FFKK72 pKa = 11.27 NPAAYY77 pKa = 10.01 DD78 pKa = 3.45 GWDD81 pKa = 3.4 EE82 pKa = 4.11 TLEE85 pKa = 4.07 EE86 pKa = 4.29 MLEE89 pKa = 4.12 EE90 pKa = 5.03 LNDD93 pKa = 3.6
Molecular weight: 11.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.818
IPC2_protein 3.668
IPC_protein 3.617
Toseland 3.427
ProMoST 3.732
Dawson 3.605
Bjellqvist 3.808
Wikipedia 3.541
Rodwell 3.452
Grimsley 3.338
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.91
Thurlkill 3.478
EMBOSS 3.541
Sillero 3.745
Patrickios 0.362
IPC_peptide 3.592
IPC2_peptide 3.719
IPC2.peptide.svr19 3.738
Protein with the highest isoelectric point:
>tr|A0A3G8FCI6|A0A3G8FCI6_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC930 OX=2365051 GN=CHPC930_0046 PE=4 SV=1
MM1 pKa = 8.06 KK2 pKa = 9.68 MILNIEE8 pKa = 4.67 PKK10 pKa = 9.3 PQTRR14 pKa = 11.84 PRR16 pKa = 11.84 FARR19 pKa = 11.84 RR20 pKa = 11.84 GNFTTTYY27 pKa = 9.4 EE28 pKa = 4.09 DD29 pKa = 3.9 KK30 pKa = 11.09 EE31 pKa = 4.08 MKK33 pKa = 9.08 IWKK36 pKa = 8.51 NKK38 pKa = 8.82 CRR40 pKa = 11.84 ILISKK45 pKa = 9.72 QYY47 pKa = 10.92 ANQPVLEE54 pKa = 4.47 GALRR58 pKa = 11.84 TSLKK62 pKa = 10.59 FFIKK66 pKa = 9.51 PPRR69 pKa = 11.84 YY70 pKa = 8.44 ISKK73 pKa = 10.31 IKK75 pKa = 10.3 RR76 pKa = 11.84 NKK78 pKa = 8.72 QALTNEE84 pKa = 4.49 TILVSKK90 pKa = 10.65 KK91 pKa = 10.54 ADD93 pKa = 3.48 LDD95 pKa = 3.89 NYY97 pKa = 9.99 IKK99 pKa = 10.92 SLLDD103 pKa = 3.27 SANGILYY110 pKa = 10.23 KK111 pKa = 10.51 DD112 pKa = 4.26 DD113 pKa = 3.7 GQIAEE118 pKa = 4.19 IYY120 pKa = 9.42 AIKK123 pKa = 10.36 VYY125 pKa = 10.73 SEE127 pKa = 4.04 SPRR130 pKa = 11.84 IEE132 pKa = 4.26 LEE134 pKa = 3.43 IEE136 pKa = 4.04 EE137 pKa = 4.49 INRR140 pKa = 11.84 EE141 pKa = 3.91 NN142 pKa = 3.66
Molecular weight: 16.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.98
IPC2_protein 9.033
IPC_protein 8.946
Toseland 9.765
ProMoST 9.428
Dawson 9.984
Bjellqvist 9.633
Wikipedia 10.131
Rodwell 10.438
Grimsley 10.043
Solomon 10.014
Lehninger 9.97
Nozaki 9.765
DTASelect 9.619
Thurlkill 9.823
EMBOSS 10.175
Sillero 9.882
Patrickios 9.765
IPC_peptide 10.014
IPC2_peptide 8.097
IPC2.peptide.svr19 7.945
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
11123
46
1593
241.8
27.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.41 ± 0.505
0.548 ± 0.12
6.725 ± 0.221
6.581 ± 0.508
4.243 ± 0.236
6.806 ± 0.492
1.447 ± 0.133
6.491 ± 0.246
8.145 ± 0.441
8.01 ± 0.457
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.472 ± 0.182
6.257 ± 0.255
2.949 ± 0.18
4.315 ± 0.203
4.037 ± 0.274
6.356 ± 0.253
6.419 ± 0.403
5.979 ± 0.274
1.528 ± 0.202
4.279 ± 0.352
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here