Streptococcus phage Javan326

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AZF2|A0A4D6AZF2_9CAUD Uncharacterized protein OS=Streptococcus phage Javan326 OX=2548108 GN=Javan326_0051 PE=4 SV=1
MM1 pKa = 7.65IIKK4 pKa = 9.99NYY6 pKa = 10.39KK7 pKa = 9.29YY8 pKa = 10.15DD9 pKa = 3.7FSSGRR14 pKa = 11.84ICYY17 pKa = 9.28TIDD20 pKa = 3.01VDD22 pKa = 4.82GYY24 pKa = 7.95EE25 pKa = 3.95QAMEE29 pKa = 4.16HH30 pKa = 6.16TKK32 pKa = 10.03TEE34 pKa = 4.15YY35 pKa = 11.24GSVQRR40 pKa = 11.84NDD42 pKa = 2.96IDD44 pKa = 5.12DD45 pKa = 4.37FLLSVEE51 pKa = 4.73NYY53 pKa = 10.28DD54 pKa = 3.55FQEE57 pKa = 5.1AEE59 pKa = 4.01AVEE62 pKa = 4.41EE63 pKa = 4.38FVDD66 pKa = 4.83FQSCLLMYY74 pKa = 10.84GIDD77 pKa = 3.53FEE79 pKa = 5.13LRR81 pKa = 11.84NEE83 pKa = 4.21VEE85 pKa = 4.03

Molecular weight: 10.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B048|A0A4D6B048_9CAUD Transcriptional repressor OS=Streptococcus phage Javan326 OX=2548108 GN=Javan326_0065 PE=4 SV=1
MM1 pKa = 7.86ANTPIKK7 pKa = 10.5PGTDD11 pKa = 2.97NQKK14 pKa = 8.42PGRR17 pKa = 11.84YY18 pKa = 8.99VEE20 pKa = 3.83VGPRR24 pKa = 11.84GGKK27 pKa = 7.12VTHH30 pKa = 6.18GHH32 pKa = 5.69TATIGKK38 pKa = 9.72GDD40 pKa = 4.1RR41 pKa = 11.84LPPTSAKK48 pKa = 10.24GNGWKK53 pKa = 10.21KK54 pKa = 9.18VV55 pKa = 3.34

Molecular weight: 5.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

11634

38

1346

171.1

19.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.98 ± 0.611

0.516 ± 0.079

6.395 ± 0.254

8.252 ± 0.557

4.298 ± 0.305

6.799 ± 0.633

1.547 ± 0.188

6.455 ± 0.217

8.372 ± 0.4

8.424 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.15

4.908 ± 0.199

2.888 ± 0.189

4.246 ± 0.206

4.495 ± 0.329

5.664 ± 0.288

5.725 ± 0.355

6.412 ± 0.249

1.307 ± 0.243

3.705 ± 0.412
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski