Antarcticibacterium arcticum
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2941 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B8YND4|A0A5B8YND4_9FLAO Crossover junction endodeoxyribonuclease RuvC OS=Antarcticibacterium arcticum OX=2585771 GN=ruvC PE=3 SV=1
MM1 pKa = 7.72 KK2 pKa = 9.86 IYY4 pKa = 10.99 YY5 pKa = 10.16 LDD7 pKa = 3.29 TCFNFGKK14 pKa = 10.84 YY15 pKa = 8.55 EE16 pKa = 4.16 GKK18 pKa = 8.17 TLEE21 pKa = 4.42 EE22 pKa = 4.56 VISIQPDD29 pKa = 3.69 YY30 pKa = 11.46 INWCILNLDD39 pKa = 4.14 HH40 pKa = 7.35 FLVDD44 pKa = 3.57 EE45 pKa = 4.75 DD46 pKa = 3.69 QLEE49 pKa = 4.23 EE50 pKa = 3.92 FLEE53 pKa = 4.51 INTSFFLSKK62 pKa = 10.39 NALMKK67 pKa = 10.83 LEE69 pKa = 4.58 IKK71 pKa = 9.46 WDD73 pKa = 3.71 EE74 pKa = 3.82 YY75 pKa = 11.73 DD76 pKa = 3.71 FNDD79 pKa = 3.42 RR80 pKa = 11.84 SSYY83 pKa = 10.79 GSEE86 pKa = 3.62 EE87 pKa = 4.37 TEE89 pKa = 4.1 WANDD93 pKa = 3.36 VQDD96 pKa = 3.91 YY97 pKa = 11.35 DD98 pKa = 3.86 DD99 pKa = 3.82 WLNTEE104 pKa = 4.61 FGDD107 pKa = 5.3 DD108 pKa = 4.2 AGTAYY113 pKa = 10.06 WNMDD117 pKa = 2.99
Molecular weight: 14.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.719
IPC_protein 3.694
Toseland 3.49
ProMoST 3.808
Dawson 3.681
Bjellqvist 3.884
Wikipedia 3.617
Rodwell 3.528
Grimsley 3.401
Solomon 3.668
Lehninger 3.63
Nozaki 3.808
DTASelect 4.012
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.821
Patrickios 0.655
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.771
Protein with the highest isoelectric point:
>tr|A0A5B8YHA0|A0A5B8YHA0_9FLAO Uncharacterized protein OS=Antarcticibacterium arcticum OX=2585771 GN=FK178_05265 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.15 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 KK13 pKa = 8.29 NKK15 pKa = 9.34 HH16 pKa = 4.03 GFRR19 pKa = 11.84 EE20 pKa = 4.05 RR21 pKa = 11.84 MASVNGRR28 pKa = 11.84 KK29 pKa = 9.21 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 KK40 pKa = 7.97 KK41 pKa = 10.73 LSVSSEE47 pKa = 3.76 NRR49 pKa = 11.84 HH50 pKa = 4.47 KK51 pKa = 10.5 HH52 pKa = 4.49
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.281
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.003
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.051
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2941
0
2941
981175
23
3243
333.6
37.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.677 ± 0.049
0.662 ± 0.017
5.251 ± 0.032
7.209 ± 0.045
5.242 ± 0.04
6.765 ± 0.043
1.816 ± 0.022
7.668 ± 0.039
7.118 ± 0.054
9.604 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.306 ± 0.022
5.726 ± 0.042
3.689 ± 0.025
3.4 ± 0.027
4.018 ± 0.032
6.201 ± 0.036
5.496 ± 0.05
6.226 ± 0.037
1.039 ± 0.016
3.887 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here