Shimwellia blattae (strain ATCC 29907 / DSM 4481 / JCM 1650 / NBRC 105725 / CDC 9005-74) (Escherichia blattae)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Shimwellia; Shimwellia blattae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3870 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2B968|I2B968_SHIBC Putative dehydrogenase OS=Shimwellia blattae (strain ATCC 29907 / DSM 4481 / JCM 1650 / NBRC 105725 / CDC 9005-74) OX=630626 GN=EBL_c19810 PE=4 SV=1
MM1 pKa = 7.88LNMLNLCAVPVSEE14 pKa = 5.75DD15 pKa = 3.36NAGDD19 pKa = 4.1LPQKK23 pKa = 10.6YY24 pKa = 8.55YY25 pKa = 9.66QEE27 pKa = 5.51KK28 pKa = 8.74LLQWATEE35 pKa = 4.15SMKK38 pKa = 10.95ADD40 pKa = 3.35LPGAFFQARR49 pKa = 11.84LLCTKK54 pKa = 10.03FPDD57 pKa = 3.7LAEE60 pKa = 4.76PGQVIVCAFHH70 pKa = 7.44DD71 pKa = 4.19VKK73 pKa = 10.69LTADD77 pKa = 3.49ADD79 pKa = 4.25YY80 pKa = 11.55EE81 pKa = 4.47DD82 pKa = 5.08FTPADD87 pKa = 3.45DD88 pKa = 5.15SYY90 pKa = 11.22PAKK93 pKa = 10.39CIEE96 pKa = 4.96AILEE100 pKa = 4.17GMDD103 pKa = 3.05WVEE106 pKa = 3.75ASVIFDD112 pKa = 3.54QEE114 pKa = 4.26SLSFEE119 pKa = 4.13WCC121 pKa = 3.38

Molecular weight: 13.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2B3P5|I2B3P5_SHIBC Putative transport protein EBL_c00120 OS=Shimwellia blattae (strain ATCC 29907 / DSM 4481 / JCM 1650 / NBRC 105725 / CDC 9005-74) OX=630626 GN=yidE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84SRR41 pKa = 11.84LTVASKK47 pKa = 11.02

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3870

0

3870

1211296

22

3095

313.0

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.153 ± 0.045

1.164 ± 0.013

5.127 ± 0.032

5.282 ± 0.038

3.719 ± 0.029

7.802 ± 0.036

2.374 ± 0.02

5.651 ± 0.034

3.698 ± 0.032

10.931 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.732 ± 0.02

3.485 ± 0.024

4.721 ± 0.027

4.523 ± 0.035

6.177 ± 0.035

5.793 ± 0.03

5.291 ± 0.028

7.027 ± 0.036

1.561 ± 0.019

2.788 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski