Prunus necrotic ringspot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q91NQ1|Q91NQ1_9BROM ATP-dependent helicase OS=Prunus necrotic ringspot virus OX=37733 PE=3 SV=1
MM1 pKa = 7.48NPLNQLLSHH10 pKa = 6.66GCSAISSALHH20 pKa = 6.39RR21 pKa = 11.84DD22 pKa = 3.17VAYY25 pKa = 8.84TQISDD30 pKa = 3.66EE31 pKa = 4.62CFDD34 pKa = 3.55SHH36 pKa = 6.35FTQISTEE43 pKa = 4.02YY44 pKa = 10.96SLTTSVRR51 pKa = 11.84KK52 pKa = 9.92LFEE55 pKa = 5.94AYY57 pKa = 8.83MVTMFLKK64 pKa = 10.5KK65 pKa = 9.59HH66 pKa = 5.03MSYY69 pKa = 10.78VFEE72 pKa = 4.88DD73 pKa = 4.92FIGSEE78 pKa = 4.05MGPYY82 pKa = 9.52VQTRR86 pKa = 11.84WRR88 pKa = 11.84IVFDD92 pKa = 3.91LDD94 pKa = 3.65EE95 pKa = 4.6VEE97 pKa = 4.48EE98 pKa = 4.66EE99 pKa = 4.42YY100 pKa = 11.07VYY102 pKa = 11.13KK103 pKa = 10.34PDD105 pKa = 3.98FYY107 pKa = 11.13HH108 pKa = 6.87YY109 pKa = 10.78DD110 pKa = 3.39VYY112 pKa = 10.99DD113 pKa = 3.64YY114 pKa = 11.71EE115 pKa = 4.46MGVDD119 pKa = 3.91IPASIMDD126 pKa = 6.05DD127 pKa = 3.3IPFCEE132 pKa = 4.47DD133 pKa = 3.44AEE135 pKa = 4.35AVTCRR140 pKa = 11.84NCGSFDD146 pKa = 3.52EE147 pKa = 4.86MEE149 pKa = 4.39TVYY152 pKa = 10.58QAATSSDD159 pKa = 3.16EE160 pKa = 4.13SRR162 pKa = 11.84ANVCDD167 pKa = 3.09VDD169 pKa = 4.8APHH172 pKa = 7.23DD173 pKa = 4.1TIKK176 pKa = 10.64WSDD179 pKa = 3.83EE180 pKa = 3.67PDD182 pKa = 3.45LTVDD186 pKa = 5.1DD187 pKa = 4.77PSKK190 pKa = 11.08LHH192 pKa = 7.11DD193 pKa = 5.38LDD195 pKa = 6.56DD196 pKa = 4.87EE197 pKa = 4.87VPQLQLEE204 pKa = 4.57TVGRR208 pKa = 11.84VVQSVEE214 pKa = 3.98PLVPDD219 pKa = 3.45INSFEE224 pKa = 4.32TNQLHH229 pKa = 5.98LVKK232 pKa = 10.4EE233 pKa = 4.43VVLDD237 pKa = 3.66RR238 pKa = 11.84FEE240 pKa = 6.52SILEE244 pKa = 3.99CHH246 pKa = 6.48EE247 pKa = 4.31KK248 pKa = 10.75VLARR252 pKa = 11.84NDD254 pKa = 3.44WKK256 pKa = 11.04SLAMYY261 pKa = 9.95VPTGEE266 pKa = 4.32IPSVSDD272 pKa = 3.06ILEE275 pKa = 4.18IPVKK279 pKa = 9.4HH280 pKa = 5.49TQPEE284 pKa = 4.95FIQMAIDD291 pKa = 3.56EE292 pKa = 4.74MLPGVSDD299 pKa = 3.64FDD301 pKa = 4.42DD302 pKa = 4.65RR303 pKa = 11.84FFQEE307 pKa = 3.89IVEE310 pKa = 4.3TSDD313 pKa = 2.94INLEE317 pKa = 3.73IDD319 pKa = 3.42RR320 pKa = 11.84ASIDD324 pKa = 3.21MSVFNDD330 pKa = 3.32WTPKK334 pKa = 9.87SGRR337 pKa = 11.84LNGLFQTGNISKK349 pKa = 10.21RR350 pKa = 11.84VPTFRR355 pKa = 11.84EE356 pKa = 3.29ASLAIKK362 pKa = 9.92KK363 pKa = 10.38RR364 pKa = 11.84NLNVPDD370 pKa = 4.15LQQVLHH376 pKa = 7.06EE377 pKa = 4.86DD378 pKa = 3.42DD379 pKa = 3.73EE380 pKa = 5.08ARR382 pKa = 11.84FIANKK387 pKa = 10.04FINTVIDD394 pKa = 4.11PNKK397 pKa = 9.5LAQFPGYY404 pKa = 9.77ISEE407 pKa = 4.62GEE409 pKa = 3.54MGYY412 pKa = 10.49FNKK415 pKa = 10.35YY416 pKa = 10.32LSGKK420 pKa = 6.74TVPDD424 pKa = 3.82DD425 pKa = 4.67AFVDD429 pKa = 4.29PCALVSMDD437 pKa = 4.87KK438 pKa = 9.87YY439 pKa = 10.69RR440 pKa = 11.84HH441 pKa = 5.51MIKK444 pKa = 8.45TQLKK448 pKa = 8.28PVEE451 pKa = 4.32DD452 pKa = 3.76TSQLFEE458 pKa = 4.66RR459 pKa = 11.84PLAATITYY467 pKa = 9.23HH468 pKa = 7.77DD469 pKa = 4.3KK470 pKa = 11.19GKK472 pKa = 10.97VMSTSPIFLMMCNRR486 pKa = 11.84LLLCLNDD493 pKa = 4.74KK494 pKa = 10.8ISIPSGKK501 pKa = 8.93HH502 pKa = 4.4HH503 pKa = 6.45QLFSLDD509 pKa = 3.38PFAFEE514 pKa = 3.71MTKK517 pKa = 10.08EE518 pKa = 4.05FKK520 pKa = 10.65EE521 pKa = 3.75IDD523 pKa = 3.38FSKK526 pKa = 10.51FDD528 pKa = 3.39KK529 pKa = 10.68SQQRR533 pKa = 11.84LHH535 pKa = 7.41HH536 pKa = 7.01LIQFHH541 pKa = 6.82IFTALGAPKK550 pKa = 10.28DD551 pKa = 3.96FLDD554 pKa = 3.35MWFASHH560 pKa = 7.23EE561 pKa = 4.05ISHH564 pKa = 6.59IRR566 pKa = 11.84DD567 pKa = 3.92GPCGIGFSVNYY578 pKa = 8.16QRR580 pKa = 11.84RR581 pKa = 11.84TGDD584 pKa = 2.69ACTYY588 pKa = 10.06LGNTIITLSALAYY601 pKa = 9.8MYY603 pKa = 10.97DD604 pKa = 3.7LLDD607 pKa = 4.26PNVTFVIASGDD618 pKa = 3.52DD619 pKa = 3.56SLIGSIKK626 pKa = 10.23PLDD629 pKa = 3.61RR630 pKa = 11.84SEE632 pKa = 4.05EE633 pKa = 4.18FKK635 pKa = 11.03FVTLFNFEE643 pKa = 4.35AKK645 pKa = 10.1FPHH648 pKa = 5.94NQPFVCSKK656 pKa = 9.39FLCLLPTVSGGKK668 pKa = 9.47KK669 pKa = 9.69VLAVPNALKK678 pKa = 10.98LNIKK682 pKa = 10.46LGVKK686 pKa = 10.14DD687 pKa = 4.02LAPCVFDD694 pKa = 3.37AWYY697 pKa = 10.7NSWLDD702 pKa = 3.99LIWYY706 pKa = 8.15FDD708 pKa = 3.46NYY710 pKa = 10.99LVVSTMKK717 pKa = 10.64DD718 pKa = 3.89YY719 pKa = 10.91ISHH722 pKa = 7.08RR723 pKa = 11.84YY724 pKa = 8.93LRR726 pKa = 11.84RR727 pKa = 11.84QTCYY731 pKa = 10.49QEE733 pKa = 4.45GAMLAYY739 pKa = 9.02RR740 pKa = 11.84TIFSSKK746 pKa = 9.72EE747 pKa = 3.6KK748 pKa = 10.32CLKK751 pKa = 10.45SLFGITSKK759 pKa = 10.84DD760 pKa = 3.58LEE762 pKa = 4.82AMLPMSKK769 pKa = 10.11NGKK772 pKa = 8.3GSNRR776 pKa = 11.84AEE778 pKa = 3.64CSKK781 pKa = 11.12NNKK784 pKa = 7.42TYY786 pKa = 10.6RR787 pKa = 11.84PPKK790 pKa = 9.0STRR793 pKa = 11.84CRR795 pKa = 11.84NSANN799 pKa = 3.3

Molecular weight: 91.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q98602|Q98602_9BROM Coat protein OS=Prunus necrotic ringspot virus OX=37733 PE=3 SV=1
MM1 pKa = 7.17VCRR4 pKa = 11.84ICNHH8 pKa = 4.64THH10 pKa = 6.79AGGCRR15 pKa = 11.84SCKK18 pKa = 9.53RR19 pKa = 11.84CHH21 pKa = 6.49PNDD24 pKa = 3.44ALVPLRR30 pKa = 11.84AQQRR34 pKa = 11.84AANNPNRR41 pKa = 11.84NRR43 pKa = 11.84NPNRR47 pKa = 11.84VSSGIGPAVRR57 pKa = 11.84PQPVVKK63 pKa = 8.13TTWTVRR69 pKa = 11.84GPNVPPRR76 pKa = 11.84IPKK79 pKa = 10.17GYY81 pKa = 8.25VAHH84 pKa = 6.08NHH86 pKa = 6.2RR87 pKa = 11.84EE88 pKa = 4.16VTTTEE93 pKa = 3.58AVKK96 pKa = 10.79YY97 pKa = 10.23LSIDD101 pKa = 3.41FTTTLPQLMGQNLTLLTVIVRR122 pKa = 11.84MNSMSSNGWIGMVEE136 pKa = 4.39DD137 pKa = 4.33YY138 pKa = 11.01KK139 pKa = 11.23VDD141 pKa = 3.88QPDD144 pKa = 3.77GPNALSRR151 pKa = 11.84KK152 pKa = 9.54GFLKK156 pKa = 10.31DD157 pKa = 4.37QPRR160 pKa = 11.84GWQFEE165 pKa = 4.36PPSDD169 pKa = 4.31LDD171 pKa = 3.35FDD173 pKa = 4.23TFARR177 pKa = 11.84THH179 pKa = 5.58RR180 pKa = 11.84VVIEE184 pKa = 4.53FKK186 pKa = 10.65TEE188 pKa = 3.59VPAGAKK194 pKa = 10.02VLVRR198 pKa = 11.84DD199 pKa = 4.43LYY201 pKa = 11.58VVVSDD206 pKa = 4.51LPRR209 pKa = 11.84VQIPTDD215 pKa = 3.44VLLVDD220 pKa = 4.27EE221 pKa = 5.62DD222 pKa = 4.01LLEE225 pKa = 4.15II226 pKa = 5.03

Molecular weight: 25.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2351

226

1045

587.8

66.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.168 ± 0.675

2.169 ± 0.105

7.188 ± 0.503

6.04 ± 0.381

4.296 ± 0.87

4.977 ± 0.443

2.382 ± 0.21

5.359 ± 0.382

6.125 ± 0.357

8.677 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.807 ± 0.339

3.828 ± 0.589

4.934 ± 0.679

3.105 ± 0.172

5.955 ± 0.924

7.741 ± 0.496

5.955 ± 0.495

8.124 ± 0.832

1.063 ± 0.073

3.105 ± 0.422
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski