Amino acid dipepetide frequency for Prunus necrotic ringspot virus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.106AlaAla: 5.106 ± 1.844
0.426AlaCys: 0.426 ± 0.348
4.255AlaAsp: 4.255 ± 2.189
3.83AlaGlu: 3.83 ± 0.851
1.702AlaPhe: 1.702 ± 0.942
4.255AlaGly: 4.255 ± 1.893
1.277AlaHis: 1.277 ± 0.66
3.404AlaIle: 3.404 ± 0.92
2.979AlaLys: 2.979 ± 1.018
6.809AlaLeu: 6.809 ± 0.547
2.553AlaMet: 2.553 ± 0.64
2.553AlaAsn: 2.553 ± 0.605
2.979AlaPro: 2.979 ± 0.72
1.702AlaGln: 1.702 ± 0.412
2.128AlaArg: 2.128 ± 0.665
4.255AlaSer: 4.255 ± 0.943
2.979AlaThr: 2.979 ± 0.588
4.681AlaVal: 4.681 ± 1.294
1.277AlaTrp: 1.277 ± 0.379
2.553AlaTyr: 2.553 ± 1.021
0.0AlaXaa: 0.0 ± 0.0
Cys
1.702CysAla: 1.702 ± 0.634
0.851CysCys: 0.851 ± 0.574
0.851CysAsp: 0.851 ± 0.471
1.277CysGlu: 1.277 ± 0.379
1.277CysPhe: 1.277 ± 0.379
1.277CysGly: 1.277 ± 0.51
1.702CysHis: 1.702 ± 0.605
0.0CysIle: 0.0 ± 0.0
0.426CysLys: 0.426 ± 0.612
1.702CysLeu: 1.702 ± 0.845
0.0CysMet: 0.0 ± 0.0
1.702CysAsn: 1.702 ± 0.412
1.702CysPro: 1.702 ± 0.831
0.0CysGln: 0.0 ± 0.0
2.979CysArg: 2.979 ± 0.823
1.702CysSer: 1.702 ± 1.041
0.426CysThr: 0.426 ± 0.348
1.702CysVal: 1.702 ± 0.327
0.0CysTrp: 0.0 ± 0.0
0.426CysTyr: 0.426 ± 0.348
0.0CysXaa: 0.0 ± 0.0
Asp
4.681AspAla: 4.681 ± 0.671
0.0AspCys: 0.0 ± 0.0
5.532AspAsp: 5.532 ± 1.435
7.66AspGlu: 7.66 ± 1.231
4.681AspPhe: 4.681 ± 1.135
3.404AspGly: 3.404 ± 1.287
1.277AspHis: 1.277 ± 0.861
4.255AspIle: 4.255 ± 0.943
4.255AspLys: 4.255 ± 0.662
9.362AspLeu: 9.362 ± 1.412
1.277AspMet: 1.277 ± 0.51
0.851AspAsn: 0.851 ± 0.471
3.83AspPro: 3.83 ± 1.131
1.277AspGln: 1.277 ± 1.145
4.681AspArg: 4.681 ± 0.864
2.979AspSer: 2.979 ± 1.004
3.404AspThr: 3.404 ± 0.387
4.681AspVal: 4.681 ± 0.788
0.851AspTrp: 0.851 ± 0.696
2.979AspTyr: 2.979 ± 0.503
0.0AspXaa: 0.0 ± 0.0
Glu
6.383GluAla: 6.383 ± 1.044
3.404GluCys: 3.404 ± 0.533
4.255GluAsp: 4.255 ± 0.934
4.681GluGlu: 4.681 ± 0.873
2.128GluPhe: 2.128 ± 0.844
1.702GluGly: 1.702 ± 0.488
0.426GluHis: 0.426 ± 0.287
5.106GluIle: 5.106 ± 1.281
3.404GluLys: 3.404 ± 0.654
2.553GluLeu: 2.553 ± 1.721
3.83GluMet: 3.83 ± 2.187
0.851GluAsn: 0.851 ± 0.787
2.979GluPro: 2.979 ± 0.287
0.851GluGln: 0.851 ± 0.411
3.83GluArg: 3.83 ± 1.643
4.255GluSer: 4.255 ± 1.391
3.83GluThr: 3.83 ± 0.682
4.681GluVal: 4.681 ± 0.868
0.0GluTrp: 0.0 ± 0.0
1.277GluTyr: 1.277 ± 0.51
0.0GluXaa: 0.0 ± 0.0
Phe
3.404PheAla: 3.404 ± 0.744
0.426PheCys: 0.426 ± 0.348
5.957PheAsp: 5.957 ± 1.212
3.83PheGlu: 3.83 ± 2.151
1.277PhePhe: 1.277 ± 0.379
1.702PheGly: 1.702 ± 0.634
0.426PheHis: 0.426 ± 0.348
2.979PheIle: 2.979 ± 0.916
2.128PheLys: 2.128 ± 0.383
3.404PheLeu: 3.404 ± 1.274
0.0PheMet: 0.0 ± 0.0
1.702PheAsn: 1.702 ± 0.845
1.702PhePro: 1.702 ± 0.488
0.851PheGln: 0.851 ± 0.696
1.277PheArg: 1.277 ± 0.379
2.979PheSer: 2.979 ± 0.918
3.404PheThr: 3.404 ± 0.744
3.83PheVal: 3.83 ± 0.682
0.426PheTrp: 0.426 ± 0.393
1.277PheTyr: 1.277 ± 0.379
0.0PheXaa: 0.0 ± 0.0
Gly
2.553GlyAla: 2.553 ± 0.424
2.553GlyCys: 2.553 ± 0.809
2.979GlyAsp: 2.979 ± 0.588
5.106GlyGlu: 5.106 ± 1.413
1.277GlyPhe: 1.277 ± 0.8
2.553GlyGly: 2.553 ± 0.764
0.426GlyHis: 0.426 ± 0.287
1.277GlyIle: 1.277 ± 0.836
4.681GlyLys: 4.681 ± 1.179
1.277GlyLeu: 1.277 ± 0.286
1.277GlyMet: 1.277 ± 0.66
2.553GlyAsn: 2.553 ± 0.382
3.83GlyPro: 3.83 ± 1.362
1.277GlyGln: 1.277 ± 0.66
2.979GlyArg: 2.979 ± 0.544
4.255GlySer: 4.255 ± 0.982
1.702GlyThr: 1.702 ± 0.831
3.83GlyVal: 3.83 ± 1.137
0.851GlyTrp: 0.851 ± 1.223
2.553GlyTyr: 2.553 ± 0.343
0.0GlyXaa: 0.0 ± 0.0
His
1.277HisAla: 1.277 ± 0.66
0.426HisCys: 0.426 ± 0.287
1.277HisAsp: 1.277 ± 1.045
1.702HisGlu: 1.702 ± 0.845
1.277HisPhe: 1.277 ± 0.379
0.426HisGly: 0.426 ± 0.348
0.851HisHis: 0.851 ± 0.696
1.702HisIle: 1.702 ± 0.488
1.702HisLys: 1.702 ± 0.831
1.702HisLeu: 1.702 ± 0.488
1.277HisMet: 1.277 ± 0.729
0.851HisAsn: 0.851 ± 0.644
1.277HisPro: 1.277 ± 0.66
0.426HisGln: 0.426 ± 0.348
2.553HisArg: 2.553 ± 0.917
1.277HisSer: 1.277 ± 0.861
2.979HisThr: 2.979 ± 1.018
0.0HisVal: 0.0 ± 0.0
0.426HisTrp: 0.426 ± 0.287
0.426HisTyr: 0.426 ± 0.348
0.0HisXaa: 0.0 ± 0.0
Ile
3.83IleAla: 3.83 ± 0.851
0.426IleCys: 0.426 ± 0.612
4.681IleAsp: 4.681 ± 0.746
1.277IleGlu: 1.277 ± 0.73
2.128IlePhe: 2.128 ± 0.707
2.128IleGly: 2.128 ± 1.451
0.851IleHis: 0.851 ± 0.574
2.128IleIle: 2.128 ± 0.616
3.404IleLys: 3.404 ± 1.254
4.681IleLeu: 4.681 ± 1.591
1.702IleMet: 1.702 ± 0.444
1.702IleAsn: 1.702 ± 0.845
5.106IlePro: 5.106 ± 0.78
2.553IleGln: 2.553 ± 0.573
2.128IleArg: 2.128 ± 0.908
6.383IleSer: 6.383 ± 1.853
2.553IleThr: 2.553 ± 0.672
3.404IleVal: 3.404 ± 0.387
0.851IleTrp: 0.851 ± 0.224
1.277IleTyr: 1.277 ± 0.589
0.0IleXaa: 0.0 ± 0.0
Lys
2.553LysAla: 2.553 ± 1.367
0.851LysCys: 0.851 ± 0.224
4.255LysAsp: 4.255 ± 0.328
2.979LysGlu: 2.979 ± 0.918
3.83LysPhe: 3.83 ± 1.131
4.255LysGly: 4.255 ± 1.369
1.702LysHis: 1.702 ± 0.845
2.128LysIle: 2.128 ± 0.545
4.255LysLys: 4.255 ± 0.805
5.106LysLeu: 5.106 ± 0.802
0.426LysMet: 0.426 ± 0.287
1.277LysAsn: 1.277 ± 0.729
3.404LysPro: 3.404 ± 0.533
1.277LysGln: 1.277 ± 0.861
3.404LysArg: 3.404 ± 0.387
4.681LysSer: 4.681 ± 0.891
5.106LysThr: 5.106 ± 0.729
5.957LysVal: 5.957 ± 1.405
1.702LysTrp: 1.702 ± 0.634
2.128LysTyr: 2.128 ± 0.665
0.0LysXaa: 0.0 ± 0.0
Leu
5.106LeuAla: 5.106 ± 2.833
1.277LeuCys: 1.277 ± 0.51
4.255LeuAsp: 4.255 ± 2.493
4.681LeuGlu: 4.681 ± 0.671
5.106LeuPhe: 5.106 ± 1.143
4.681LeuGly: 4.681 ± 0.873
3.404LeuHis: 3.404 ± 1.512
3.404LeuIle: 3.404 ± 0.533
3.404LeuLys: 3.404 ± 0.867
7.234LeuLeu: 7.234 ± 0.907
3.404LeuMet: 3.404 ± 0.891
4.255LeuAsn: 4.255 ± 1.065
5.106LeuPro: 5.106 ± 0.794
4.681LeuGln: 4.681 ± 1.672
5.106LeuArg: 5.106 ± 2.026
6.809LeuSer: 6.809 ± 1.334
5.532LeuThr: 5.532 ± 1.442
8.085LeuVal: 8.085 ± 1.374
0.0LeuTrp: 0.0 ± 0.0
1.277LeuTyr: 1.277 ± 0.73
0.0LeuXaa: 0.0 ± 0.0
Met
2.128MetAla: 2.128 ± 0.545
0.851MetCys: 0.851 ± 0.224
2.979MetAsp: 2.979 ± 0.544
1.277MetGlu: 1.277 ± 0.286
0.426MetPhe: 0.426 ± 0.348
2.128MetGly: 2.128 ± 0.844
0.0MetHis: 0.0 ± 0.0
1.277MetIle: 1.277 ± 0.379
0.426MetLys: 0.426 ± 0.348
2.553MetLeu: 2.553 ± 0.596
1.277MetMet: 1.277 ± 0.286
1.277MetAsn: 1.277 ± 0.458
0.0MetPro: 0.0 ± 0.0
0.0MetGln: 0.0 ± 0.0
0.426MetArg: 0.426 ± 0.393
5.532MetSer: 5.532 ± 1.153
1.277MetThr: 1.277 ± 0.379
2.553MetVal: 2.553 ± 1.075
0.426MetTrp: 0.426 ± 0.348
1.277MetTyr: 1.277 ± 0.51
0.0MetXaa: 0.0 ± 0.0
Asn
1.277AsnAla: 1.277 ± 0.458
0.426AsnCys: 0.426 ± 0.348
2.553AsnAsp: 2.553 ± 0.872
0.851AsnGlu: 0.851 ± 0.574
2.128AsnPhe: 2.128 ± 0.908
2.128AsnGly: 2.128 ± 0.665
1.277AsnHis: 1.277 ± 1.378
2.979AsnIle: 2.979 ± 0.544
2.553AsnLys: 2.553 ± 1.021
3.404AsnLeu: 3.404 ± 0.597
0.851AsnMet: 0.851 ± 0.249
0.851AsnAsn: 0.851 ± 0.644
2.553AsnPro: 2.553 ± 1.41
1.277AsnGln: 1.277 ± 1.045
3.404AsnArg: 3.404 ± 1.938
2.553AsnSer: 2.553 ± 0.872
1.277AsnThr: 1.277 ± 0.51
4.255AsnVal: 4.255 ± 0.79
0.426AsnTrp: 0.426 ± 0.287
1.277AsnTyr: 1.277 ± 0.51
0.0AsnXaa: 0.0 ± 0.0
Pro
2.553ProAla: 2.553 ± 0.967
2.128ProCys: 2.128 ± 0.707
4.681ProAsp: 4.681 ± 0.671
2.553ProGlu: 2.553 ± 0.807
1.702ProPhe: 1.702 ± 0.845
2.553ProGly: 2.553 ± 0.382
1.702ProHis: 1.702 ± 0.448
2.979ProIle: 2.979 ± 1.362
5.957ProLys: 5.957 ± 1.089
4.255ProLeu: 4.255 ± 0.328
0.426ProMet: 0.426 ± 0.348
5.106ProAsn: 5.106 ± 2.458
1.702ProPro: 1.702 ± 1.057
1.277ProGln: 1.277 ± 1.207
1.702ProArg: 1.702 ± 1.746
2.979ProSer: 2.979 ± 1.057
2.979ProThr: 2.979 ± 0.587
1.277ProVal: 1.277 ± 0.836
0.0ProTrp: 0.0 ± 0.0
0.851ProTyr: 0.851 ± 0.224
0.0ProXaa: 0.0 ± 0.0
Gln
2.553GlnAla: 2.553 ± 0.708
0.426GlnCys: 0.426 ± 0.287
0.0GlnAsp: 0.0 ± 0.0
1.277GlnGlu: 1.277 ± 0.51
2.128GlnPhe: 2.128 ± 0.383
1.277GlnGly: 1.277 ± 0.861
0.851GlnHis: 0.851 ± 0.574
2.979GlnIle: 2.979 ± 0.287
2.553GlnLys: 2.553 ± 1.367
4.255GlnLeu: 4.255 ± 1.915
0.426GlnMet: 0.426 ± 0.348
1.277GlnAsn: 1.277 ± 0.73
2.979GlnPro: 2.979 ± 1.759
2.128GlnGln: 2.128 ± 0.665
2.128GlnArg: 2.128 ± 0.383
0.426GlnSer: 0.426 ± 0.348
1.702GlnThr: 1.702 ± 1.041
0.851GlnVal: 0.851 ± 0.224
0.0GlnTrp: 0.0 ± 0.0
0.851GlnTyr: 0.851 ± 0.574
0.0GlnXaa: 0.0 ± 0.0
Arg
3.83ArgAla: 3.83 ± 1.223
1.702ArgCys: 1.702 ± 0.605
5.106ArgAsp: 5.106 ± 1.844
2.553ArgGlu: 2.553 ± 0.764
2.979ArgPhe: 2.979 ± 0.753
1.702ArgGly: 1.702 ± 1.136
0.851ArgHis: 0.851 ± 0.224
2.553ArgIle: 2.553 ± 1.075
2.128ArgLys: 2.128 ± 0.508
5.532ArgLeu: 5.532 ± 1.368
1.702ArgMet: 1.702 ± 0.76
4.255ArgAsn: 4.255 ± 0.766
2.553ArgPro: 2.553 ± 0.424
2.553ArgGln: 2.553 ± 0.708
2.979ArgArg: 2.979 ± 1.004
4.681ArgSer: 4.681 ± 1.496
3.83ArgThr: 3.83 ± 1.009
5.532ArgVal: 5.532 ± 1.553
1.277ArgTrp: 1.277 ± 0.286
1.277ArgTyr: 1.277 ± 0.379
0.0ArgXaa: 0.0 ± 0.0
Ser
4.255SerAla: 4.255 ± 0.662
2.128SerCys: 2.128 ± 0.887
8.085SerAsp: 8.085 ± 0.929
2.979SerGlu: 2.979 ± 0.487
2.128SerPhe: 2.128 ± 0.552
4.681SerGly: 4.681 ± 0.977
2.979SerHis: 2.979 ± 1.351
4.681SerIle: 4.681 ± 0.671
5.532SerLys: 5.532 ± 2.039
5.957SerLeu: 5.957 ± 1.141
2.979SerMet: 2.979 ± 0.802
2.979SerAsn: 2.979 ± 0.802
0.426SerPro: 0.426 ± 0.348
2.553SerGln: 2.553 ± 0.382
5.106SerArg: 5.106 ± 1.613
6.383SerSer: 6.383 ± 1.359
4.681SerThr: 4.681 ± 0.864
6.383SerVal: 6.383 ± 1.068
1.277SerTrp: 1.277 ± 0.379
1.277SerTyr: 1.277 ± 0.379
0.0SerXaa: 0.0 ± 0.0
Thr
2.553ThrAla: 2.553 ± 1.188
0.851ThrCys: 0.851 ± 0.696
2.553ThrAsp: 2.553 ± 1.078
3.404ThrGlu: 3.404 ± 1.08
1.277ThrPhe: 1.277 ± 0.836
2.979ThrGly: 2.979 ± 0.487
1.702ThrHis: 1.702 ± 1.136
2.979ThrIle: 2.979 ± 0.918
4.255ThrLys: 4.255 ± 1.622
4.681ThrLeu: 4.681 ± 1.294
1.702ThrMet: 1.702 ± 0.448
1.702ThrAsn: 1.702 ± 0.634
0.426ThrPro: 0.426 ± 0.348
2.979ThrGln: 2.979 ± 0.916
2.979ThrArg: 2.979 ± 1.004
5.106ThrSer: 5.106 ± 1.191
5.957ThrThr: 5.957 ± 2.756
8.085ThrVal: 8.085 ± 0.321
0.426ThrTrp: 0.426 ± 0.612
2.979ThrTyr: 2.979 ± 0.487
0.0ThrXaa: 0.0 ± 0.0
Val
4.681ValAla: 4.681 ± 2.121
1.702ValCys: 1.702 ± 0.583
4.681ValAsp: 4.681 ± 0.668
6.383ValGlu: 6.383 ± 0.965
3.404ValPhe: 3.404 ± 0.756
4.681ValGly: 4.681 ± 1.694
1.277ValHis: 1.277 ± 0.861
3.83ValIle: 3.83 ± 0.837
5.532ValLys: 5.532 ± 1.495
7.234ValLeu: 7.234 ± 0.989
1.277ValMet: 1.277 ± 0.379
1.702ValAsn: 1.702 ± 0.651
6.383ValPro: 6.383 ± 1.706
1.277ValGln: 1.277 ± 0.836
5.957ValArg: 5.957 ± 2.355
7.234ValSer: 7.234 ± 1.269
3.83ValThr: 3.83 ± 0.993
6.809ValVal: 6.809 ± 1.873
0.0ValTrp: 0.0 ± 0.0
3.404ValTyr: 3.404 ± 0.533
0.0ValXaa: 0.0 ± 0.0
Trp
0.426TrpAla: 0.426 ± 0.287
0.0TrpCys: 0.0 ± 0.0
0.426TrpAsp: 0.426 ± 0.393
0.426TrpGlu: 0.426 ± 0.287
0.851TrpPhe: 0.851 ± 0.224
0.426TrpGly: 0.426 ± 0.393
0.0TrpHis: 0.0 ± 0.0
0.851TrpIle: 0.851 ± 0.568
0.426TrpLys: 0.426 ± 0.348
0.851TrpLeu: 0.851 ± 0.224
0.0TrpMet: 0.0 ± 0.0
0.0TrpAsn: 0.0 ± 0.0
0.0TrpPro: 0.0 ± 0.0
0.426TrpGln: 0.426 ± 0.612
0.426TrpArg: 0.426 ± 0.348
2.128TrpSer: 2.128 ± 0.908
1.277TrpThr: 1.277 ± 0.458
0.0TrpVal: 0.0 ± 0.0
0.426TrpTrp: 0.426 ± 0.287
1.277TrpTyr: 1.277 ± 0.51
0.0TrpXaa: 0.0 ± 0.0
Tyr
0.851TyrAla: 0.851 ± 0.574
0.851TyrCys: 0.851 ± 0.574
2.553TyrAsp: 2.553 ± 0.672
1.702TyrGlu: 1.702 ± 0.634
1.277TyrPhe: 1.277 ± 0.51
0.851TyrGly: 0.851 ± 0.574
0.851TyrHis: 0.851 ± 0.696
2.128TyrIle: 2.128 ± 0.552
1.277TyrLys: 1.277 ± 0.458
4.255TyrLeu: 4.255 ± 0.716
1.277TyrMet: 1.277 ± 0.51
0.851TyrAsn: 0.851 ± 0.224
0.426TyrPro: 0.426 ± 0.287
1.702TyrGln: 1.702 ± 0.845
3.404TyrArg: 3.404 ± 0.533
0.851TyrSer: 0.851 ± 0.471
0.851TyrThr: 0.851 ± 0.224
4.681TyrVal: 4.681 ± 0.706
0.0TyrTrp: 0.0 ± 0.0
0.426TyrTyr: 0.426 ± 0.287
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4 proteins (2351 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski