Motilibacter peucedani
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3836 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A420XPH0|A0A420XPH0_9ACTN CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase OS=Motilibacter peucedani OX=598650 GN=CLV35_2587 PE=4 SV=1
MM1 pKa = 7.3 SHH3 pKa = 7.55 DD4 pKa = 4.5 PVQAWASVEE13 pKa = 3.85 AWLGAHH19 pKa = 7.35 APRR22 pKa = 11.84 SAGVLSPPADD32 pKa = 3.75 PASIAHH38 pKa = 6.61 AEE40 pKa = 4.31 GVTGVPWPDD49 pKa = 3.07 ALKK52 pKa = 9.7 TLLLAHH58 pKa = 7.17 DD59 pKa = 5.03 GVDD62 pKa = 4.83 DD63 pKa = 5.09 PYY65 pKa = 11.74 DD66 pKa = 3.93 DD67 pKa = 4.36 ASGIFFGAPLLSVQGILIALEE88 pKa = 3.84 QLVVVAGVMDD98 pKa = 4.74 DD99 pKa = 4.08 FGIPAAMDD107 pKa = 3.99 PAAGGPVGHH116 pKa = 5.87 YY117 pKa = 9.99 VPWLLPFAGLEE128 pKa = 4.06 GSFWVLDD135 pKa = 3.85 LRR137 pKa = 11.84 PGEE140 pKa = 4.06 QSGCVCYY147 pKa = 10.06 YY148 pKa = 10.89 SPVEE152 pKa = 4.03 QLVAPAAAASRR163 pKa = 11.84 VEE165 pKa = 4.47 FTAQLALCLSTSAPWNGVLRR185 pKa = 11.84 PFVTDD190 pKa = 4.62 GALDD194 pKa = 3.6 WAEE197 pKa = 3.84 LDD199 pKa = 3.44 AAEE202 pKa = 4.87 VVDD205 pKa = 5.6 LRR207 pKa = 4.71
Molecular weight: 21.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.961
IPC_protein 3.948
Toseland 3.732
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.884
Rodwell 3.77
Grimsley 3.643
Solomon 3.935
Lehninger 3.884
Nozaki 4.062
DTASelect 4.291
Thurlkill 3.795
EMBOSS 3.884
Sillero 4.075
Patrickios 1.926
IPC_peptide 3.923
IPC2_peptide 4.05
IPC2.peptide.svr19 3.951
Protein with the highest isoelectric point:
>tr|A0A420XPQ3|A0A420XPQ3_9ACTN ADP-ribose pyrophosphatase YjhB (NUDIX family) OS=Motilibacter peucedani OX=598650 GN=CLV35_1699 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.7 LLRR22 pKa = 11.84 KK23 pKa = 7.97 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.81 KK32 pKa = 9.63
Molecular weight: 4.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3836
0
3836
1277826
29
1848
333.1
35.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.518 ± 0.073
0.677 ± 0.01
5.955 ± 0.031
5.317 ± 0.044
2.513 ± 0.024
9.454 ± 0.035
2.032 ± 0.015
2.418 ± 0.026
1.639 ± 0.029
10.789 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.459 ± 0.013
1.472 ± 0.025
5.876 ± 0.033
2.719 ± 0.022
7.952 ± 0.046
5.769 ± 0.035
6.013 ± 0.045
10.038 ± 0.039
1.514 ± 0.017
1.874 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here