[Ruminococcus] torques L2-14

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Mediterraneibacter; [Ruminococcus] torques

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2797 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4M174|D4M174_9FIRM Membrane proteins related to metalloendopeptidases OS=[Ruminococcus] torques L2-14 OX=657313 GN=RTO_01980 PE=4 SV=1
MM1 pKa = 7.43NKK3 pKa = 9.65NEE5 pKa = 4.28RR6 pKa = 11.84PAFLKK11 pKa = 10.98GKK13 pKa = 8.82GAAVGAVLCFVAVITMVGSYY33 pKa = 9.43TYY35 pKa = 11.07NHH37 pKa = 6.28YY38 pKa = 10.66KK39 pKa = 10.34KK40 pKa = 10.79DD41 pKa = 3.53MEE43 pKa = 4.2QQIKK47 pKa = 10.17DD48 pKa = 3.52AQTTAEE54 pKa = 4.79QLTEE58 pKa = 4.63DD59 pKa = 4.84DD60 pKa = 3.87STLANTDD67 pKa = 4.52DD68 pKa = 4.63ILPTDD73 pKa = 3.74TDD75 pKa = 3.87SEE77 pKa = 4.16EE78 pKa = 4.9DD79 pKa = 3.28NAEE82 pKa = 4.05NQEE85 pKa = 4.16SQKK88 pKa = 11.04DD89 pKa = 3.89VQQEE93 pKa = 4.29NEE95 pKa = 3.62NSMEE99 pKa = 4.24NGNTSTEE106 pKa = 4.46EE107 pKa = 3.97IQSTASSNTQNVWFTQEE124 pKa = 4.04STLAWPASGATLLSFSMDD142 pKa = 2.91HH143 pKa = 5.45TVYY146 pKa = 10.58FPTLEE151 pKa = 3.96QYY153 pKa = 10.61KK154 pKa = 10.05YY155 pKa = 10.88NPALIIGGTQGEE167 pKa = 4.73VIHH170 pKa = 6.62AAAAGVVEE178 pKa = 5.32SIEE181 pKa = 4.02QTAQTGTTVTLDD193 pKa = 3.12MGNGYY198 pKa = 7.38TAVYY202 pKa = 9.45GQLDD206 pKa = 3.93EE207 pKa = 4.38VAAAVGDD214 pKa = 3.93YY215 pKa = 10.14IAAGEE220 pKa = 4.15EE221 pKa = 4.35VGTLNSPTKK230 pKa = 9.17YY231 pKa = 10.6YY232 pKa = 10.77SVEE235 pKa = 3.89GPNLYY240 pKa = 10.48FEE242 pKa = 4.39IMKK245 pKa = 10.56DD246 pKa = 3.68GAPVDD251 pKa = 3.54PMNFMEE257 pKa = 4.9

Molecular weight: 27.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4M1C3|D4M1C3_9FIRM Predicted RNA-binding protein OS=[Ruminococcus] torques L2-14 OX=657313 GN=RTO_02520 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2797

0

2797

851199

14

2957

304.3

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.267 ± 0.043

1.482 ± 0.018

5.597 ± 0.04

8.055 ± 0.057

3.993 ± 0.038

7.02 ± 0.041

1.775 ± 0.022

7.427 ± 0.049

7.342 ± 0.041

8.846 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.172 ± 0.027

4.374 ± 0.031

3.195 ± 0.021

3.377 ± 0.028

4.276 ± 0.043

5.524 ± 0.039

5.373 ± 0.037

6.961 ± 0.04

0.889 ± 0.018

4.055 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski