Microbacterium ketosireducens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia;

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2GVJ6|A0A0M2GVJ6_9MICO Major Facilitator Superfamily protein OS=Microbacterium ketosireducens OX=92835 GN=RS81_03299 PE=4 SV=1
MM1 pKa = 7.33FRR3 pKa = 11.84RR4 pKa = 11.84SLIAVTAAATALVLASCSAGGNPEE28 pKa = 3.97PTDD31 pKa = 3.88DD32 pKa = 3.4EE33 pKa = 4.4AARR36 pKa = 11.84GDD38 pKa = 4.04RR39 pKa = 11.84LTLISISSPTNYY51 pKa = 10.4DD52 pKa = 3.33LGVGAEE58 pKa = 4.07AGTRR62 pKa = 11.84SQFFEE67 pKa = 4.36AVFDD71 pKa = 3.86TLLRR75 pKa = 11.84ADD77 pKa = 3.76SAGEE81 pKa = 3.64ISPRR85 pKa = 11.84LATDD89 pKa = 3.62WSYY92 pKa = 12.27DD93 pKa = 3.54DD94 pKa = 4.34TLTEE98 pKa = 3.85LTLTLRR104 pKa = 11.84EE105 pKa = 4.48DD106 pKa = 3.58VTFSDD111 pKa = 4.29GTALDD116 pKa = 3.58AAAVVASLEE125 pKa = 4.15HH126 pKa = 5.99TRR128 pKa = 11.84DD129 pKa = 3.44GSSQAAGAAVGQTYY143 pKa = 9.98EE144 pKa = 4.35AVDD147 pKa = 4.26DD148 pKa = 4.02YY149 pKa = 10.72TVKK152 pKa = 9.4ITLPAPNPSYY162 pKa = 10.68LGSLAVTSGLIAAPSSFDD180 pKa = 3.94NDD182 pKa = 4.13DD183 pKa = 3.22VDD185 pKa = 4.31TNPVGSGPFILDD197 pKa = 3.38TEE199 pKa = 4.59STVIGTTYY207 pKa = 10.97NYY209 pKa = 7.65TANPDD214 pKa = 3.22YY215 pKa = 10.27WDD217 pKa = 4.47PEE219 pKa = 4.52SIRR222 pKa = 11.84YY223 pKa = 8.34DD224 pKa = 3.24HH225 pKa = 6.47LTINTIADD233 pKa = 3.39PTATLNAILAGEE245 pKa = 4.4TNGAVADD252 pKa = 4.01NNALEE257 pKa = 4.67EE258 pKa = 4.23IEE260 pKa = 4.17GAGWDD265 pKa = 3.78FTEE268 pKa = 6.35SEE270 pKa = 4.59GAWEE274 pKa = 4.55GLMLYY279 pKa = 10.59DD280 pKa = 4.92RR281 pKa = 11.84DD282 pKa = 3.81GAMAPALADD291 pKa = 3.21VRR293 pKa = 11.84VRR295 pKa = 11.84QAINMAFDD303 pKa = 3.82RR304 pKa = 11.84EE305 pKa = 4.37SLLEE309 pKa = 4.03AVQGGHH315 pKa = 5.38GTVTTQIFMPNTSTYY330 pKa = 11.0DD331 pKa = 3.49PEE333 pKa = 4.74LDD335 pKa = 3.86KK336 pKa = 10.88MYY338 pKa = 10.61AYY340 pKa = 10.4DD341 pKa = 3.8PEE343 pKa = 4.65GAKK346 pKa = 10.33EE347 pKa = 4.0LLTEE351 pKa = 4.66AGYY354 pKa = 9.92PDD356 pKa = 4.96GFTMTMPLIAAFQTSLDD373 pKa = 3.84LLKK376 pKa = 10.7QQLADD381 pKa = 3.32VGITVEE387 pKa = 4.55YY388 pKa = 10.35VDD390 pKa = 4.86PGQGQYY396 pKa = 9.68IASILTPKK404 pKa = 10.4HH405 pKa = 4.87PAAWMNMAGIGDD417 pKa = 4.07WDD419 pKa = 4.59AIGYY423 pKa = 7.07MVSPAAVFNPFRR435 pKa = 11.84TQQDD439 pKa = 3.47QVDD442 pKa = 3.54DD443 pKa = 3.93WLYY446 pKa = 10.59EE447 pKa = 3.97IQNATDD453 pKa = 3.37EE454 pKa = 4.97ADD456 pKa = 4.46ASMTDD461 pKa = 3.37LKK463 pKa = 11.24AYY465 pKa = 10.08LVEE468 pKa = 4.41EE469 pKa = 4.01AWFAPFYY476 pKa = 10.39RR477 pKa = 11.84VNGNYY482 pKa = 7.36TTDD485 pKa = 3.39AEE487 pKa = 4.27TDD489 pKa = 3.4YY490 pKa = 11.57DD491 pKa = 3.76ASTWVSGAYY500 pKa = 9.46PSIFGFSPASS510 pKa = 3.29

Molecular weight: 54.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2H233|A0A0M2H233_9MICO Putative inactive lipase OS=Microbacterium ketosireducens OX=92835 GN=RS81_03385 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3448

0

3448

1191655

29

3088

345.6

36.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.201 ± 0.063

0.467 ± 0.01

6.586 ± 0.038

5.493 ± 0.041

3.145 ± 0.027

8.985 ± 0.032

1.966 ± 0.022

4.539 ± 0.034

1.711 ± 0.025

9.831 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.759 ± 0.019

1.789 ± 0.024

5.43 ± 0.028

2.578 ± 0.018

7.219 ± 0.05

5.426 ± 0.028

6.124 ± 0.042

9.156 ± 0.042

1.603 ± 0.019

1.992 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski