Amino acid dipepetide frequency for Microbacterium ketosireducens

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.009AlaAla: 23.009 ± 0.23	0.661AlaCys: 0.661 ± 0.029	9.355AlaAsp: 9.355 ± 0.11	8.276AlaGlu: 8.276 ± 0.108	4.131AlaPhe: 4.131 ± 0.057	12.879AlaGly: 12.879 ± 0.128	2.492AlaHis: 2.492 ± 0.053	6.144AlaIle: 6.144 ± 0.085	2.461AlaLys: 2.461 ± 0.052	14.474AlaLeu: 14.474 ± 0.145	2.723AlaMet: 2.723 ± 0.046	2.184AlaAsn: 2.184 ± 0.048	7.076AlaPro: 7.076 ± 0.094	3.686AlaGln: 3.686 ± 0.066	9.57AlaArg: 9.57 ± 0.117	7.636AlaSer: 7.636 ± 0.077	7.847AlaThr: 7.847 ± 0.097	12.773AlaVal: 12.773 ± 0.14	2.111AlaTrp: 2.111 ± 0.047	2.523AlaTyr: 2.523 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.677CysAla: 0.677 ± 0.026	0.046CysCys: 0.046 ± 0.007	0.303CysAsp: 0.303 ± 0.017	0.242CysGlu: 0.242 ± 0.015	0.156CysPhe: 0.156 ± 0.012	0.493CysGly: 0.493 ± 0.024	0.108CysHis: 0.108 ± 0.009	0.185CysIle: 0.185 ± 0.012	0.052CysLys: 0.052 ± 0.006	0.399CysLeu: 0.399 ± 0.019	0.064CysMet: 0.064 ± 0.007	0.088CysAsn: 0.088 ± 0.01	0.261CysPro: 0.261 ± 0.015	0.11CysGln: 0.11 ± 0.011	0.31CysArg: 0.31 ± 0.018	0.3CysSer: 0.3 ± 0.016	0.321CysThr: 0.321 ± 0.026	0.397CysVal: 0.397 ± 0.017	0.062CysTrp: 0.062 ± 0.007	0.097CysTyr: 0.097 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	10.549AspAla: 10.549 ± 0.105	0.235AspCys: 0.235 ± 0.014	4.95AspAsp: 4.95 ± 0.083	4.58AspGlu: 4.58 ± 0.07	1.737AspPhe: 1.737 ± 0.039	6.61AspGly: 6.61 ± 0.099	1.201AspHis: 1.201 ± 0.037	2.528AspIle: 2.528 ± 0.046	0.907AspLys: 0.907 ± 0.03	6.453AspLeu: 6.453 ± 0.071	0.925AspMet: 0.925 ± 0.023	0.908AspAsn: 0.908 ± 0.033	4.367AspPro: 4.367 ± 0.073	1.434AspGln: 1.434 ± 0.034	4.67AspArg: 4.67 ± 0.081	2.547AspSer: 2.547 ± 0.051	3.138AspThr: 3.138 ± 0.058	5.758AspVal: 5.758 ± 0.081	1.051AspTrp: 1.051 ± 0.032	1.315AspTyr: 1.315 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	7.23GluAla: 7.23 ± 0.093	0.228GluCys: 0.228 ± 0.016	2.63GluAsp: 2.63 ± 0.061	2.692GluGlu: 2.692 ± 0.053	1.707GluPhe: 1.707 ± 0.033	3.866GluGly: 3.866 ± 0.067	1.423GluHis: 1.423 ± 0.037	2.705GluIle: 2.705 ± 0.053	1.221GluLys: 1.221 ± 0.038	6.08GluLeu: 6.08 ± 0.083	0.886GluMet: 0.886 ± 0.028	1.17GluAsn: 1.17 ± 0.028	3.175GluPro: 3.175 ± 0.061	2.04GluGln: 2.04 ± 0.045	5.091GluArg: 5.091 ± 0.08	2.924GluSer: 2.924 ± 0.049	3.09GluThr: 3.09 ± 0.054	4.653GluVal: 4.653 ± 0.065	0.952GluTrp: 0.952 ± 0.029	1.17GluTyr: 1.17 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.62PheAla: 4.62 ± 0.059	0.145PheCys: 0.145 ± 0.011	2.539PheAsp: 2.539 ± 0.05	1.73PheGlu: 1.73 ± 0.038	1.09PhePhe: 1.09 ± 0.033	3.374PheGly: 3.374 ± 0.055	0.556PheHis: 0.556 ± 0.023	1.205PheIle: 1.205 ± 0.035	0.382PheLys: 0.382 ± 0.016	2.81PheLeu: 2.81 ± 0.059	0.433PheMet: 0.433 ± 0.021	0.64PheAsn: 0.64 ± 0.025	1.379PhePro: 1.379 ± 0.033	0.783PheGln: 0.783 ± 0.025	1.795PheArg: 1.795 ± 0.035	1.672PheSer: 1.672 ± 0.04	2.18PheThr: 2.18 ± 0.043	2.918PheVal: 2.918 ± 0.051	0.545PheTrp: 0.545 ± 0.024	0.646PheTyr: 0.646 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	11.591GlyAla: 11.591 ± 0.111	0.542GlyCys: 0.542 ± 0.022	5.785GlyAsp: 5.785 ± 0.082	5.049GlyGlu: 5.049 ± 0.068	3.343GlyPhe: 3.343 ± 0.057	7.905GlyGly: 7.905 ± 0.108	1.742GlyHis: 1.742 ± 0.036	4.902GlyIle: 4.902 ± 0.072	1.822GlyLys: 1.822 ± 0.046	8.518GlyLeu: 8.518 ± 0.094	1.938GlyMet: 1.938 ± 0.047	1.511GlyAsn: 1.511 ± 0.045	3.739GlyPro: 3.739 ± 0.058	2.193GlyGln: 2.193 ± 0.045	6.321GlyArg: 6.321 ± 0.087	5.2GlySer: 5.2 ± 0.076	5.574GlyThr: 5.574 ± 0.094	8.217GlyVal: 8.217 ± 0.092	1.713GlyTrp: 1.713 ± 0.044	2.243GlyTyr: 2.243 ± 0.039	0.0GlyXaa: 0.0 ± 0.0
His	2.453HisAla: 2.453 ± 0.047	0.093HisCys: 0.093 ± 0.008	1.34HisAsp: 1.34 ± 0.033	1.078HisGlu: 1.078 ± 0.035	0.588HisPhe: 0.588 ± 0.023	1.996HisGly: 1.996 ± 0.039	0.569HisHis: 0.569 ± 0.023	0.742HisIle: 0.742 ± 0.023	0.232HisLys: 0.232 ± 0.013	1.927HisLeu: 1.927 ± 0.041	0.299HisMet: 0.299 ± 0.016	0.352HisAsn: 0.352 ± 0.018	1.601HisPro: 1.601 ± 0.04	0.46HisGln: 0.46 ± 0.02	1.588HisArg: 1.588 ± 0.035	0.971HisSer: 0.971 ± 0.03	0.973HisThr: 0.973 ± 0.025	1.698HisVal: 1.698 ± 0.038	0.299HisTrp: 0.299 ± 0.017	0.397HisTyr: 0.397 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.645IleAla: 7.645 ± 0.088	0.227IleCys: 0.227 ± 0.013	3.638IleAsp: 3.638 ± 0.056	2.806IleGlu: 2.806 ± 0.052	1.186IlePhe: 1.186 ± 0.033	4.714IleGly: 4.714 ± 0.075	0.708IleHis: 0.708 ± 0.025	1.76IleIle: 1.76 ± 0.054	0.628IleLys: 0.628 ± 0.027	3.696IleLeu: 3.696 ± 0.069	0.6IleMet: 0.6 ± 0.023	0.806IleAsn: 0.806 ± 0.031	2.502IlePro: 2.502 ± 0.048	0.896IleGln: 0.896 ± 0.028	2.557IleArg: 2.557 ± 0.046	2.18IleSer: 2.18 ± 0.044	2.797IleThr: 2.797 ± 0.059	4.816IleVal: 4.816 ± 0.078	0.502IleTrp: 0.502 ± 0.021	0.723IleTyr: 0.723 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	2.058LysAla: 2.058 ± 0.056	0.059LysCys: 0.059 ± 0.007	0.969LysAsp: 0.969 ± 0.029	0.78LysGlu: 0.78 ± 0.03	0.41LysPhe: 0.41 ± 0.019	1.332LysGly: 1.332 ± 0.042	0.383LysHis: 0.383 ± 0.016	0.779LysIle: 0.779 ± 0.03	0.609LysLys: 0.609 ± 0.031	1.61LysLeu: 1.61 ± 0.039	0.308LysMet: 0.308 ± 0.017	0.409LysAsn: 0.409 ± 0.019	1.065LysPro: 1.065 ± 0.032	0.604LysGln: 0.604 ± 0.025	1.417LysArg: 1.417 ± 0.042	0.967LysSer: 0.967 ± 0.033	1.238LysThr: 1.238 ± 0.035	1.486LysVal: 1.486 ± 0.042	0.239LysTrp: 0.239 ± 0.015	0.39LysTyr: 0.39 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	14.785LeuAla: 14.785 ± 0.145	0.482LeuCys: 0.482 ± 0.02	6.764LeuAsp: 6.764 ± 0.082	5.041LeuGlu: 5.041 ± 0.066	2.897LeuPhe: 2.897 ± 0.053	8.878LeuGly: 8.878 ± 0.097	1.819LeuHis: 1.819 ± 0.046	4.312LeuIle: 4.312 ± 0.077	1.469LeuLys: 1.469 ± 0.037	9.526LeuLeu: 9.526 ± 0.149	1.549LeuMet: 1.549 ± 0.04	1.55LeuAsn: 1.55 ± 0.038	5.429LeuPro: 5.429 ± 0.072	2.348LeuGln: 2.348 ± 0.046	7.285LeuArg: 7.285 ± 0.092	5.413LeuSer: 5.413 ± 0.074	6.269LeuThr: 6.269 ± 0.087	9.393LeuVal: 9.393 ± 0.118	1.408LeuTrp: 1.408 ± 0.034	1.696LeuTyr: 1.696 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.029MetAla: 2.029 ± 0.044	0.093MetCys: 0.093 ± 0.009	0.811MetAsp: 0.811 ± 0.027	0.549MetGlu: 0.549 ± 0.023	0.556MetPhe: 0.556 ± 0.024	1.229MetGly: 1.229 ± 0.039	0.335MetHis: 0.335 ± 0.016	0.829MetIle: 0.829 ± 0.034	0.397MetLys: 0.397 ± 0.019	2.021MetLeu: 2.021 ± 0.044	0.3MetMet: 0.3 ± 0.017	0.449MetAsn: 0.449 ± 0.019	1.144MetPro: 1.144 ± 0.033	0.523MetGln: 0.523 ± 0.022	1.398MetArg: 1.398 ± 0.034	1.389MetSer: 1.389 ± 0.032	1.735MetThr: 1.735 ± 0.039	1.276MetVal: 1.276 ± 0.033	0.235MetTrp: 0.235 ± 0.015	0.295MetTyr: 0.295 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.31AsnAla: 2.31 ± 0.052	0.097AsnCys: 0.097 ± 0.01	1.166AsnAsp: 1.166 ± 0.03	0.858AsnGlu: 0.858 ± 0.027	0.59AsnPhe: 0.59 ± 0.026	1.705AsnGly: 1.705 ± 0.05	0.373AsnHis: 0.373 ± 0.016	0.786AsnIle: 0.786 ± 0.024	0.314AsnLys: 0.314 ± 0.017	1.749AsnLeu: 1.749 ± 0.036	0.286AsnMet: 0.286 ± 0.018	0.397AsnAsn: 0.397 ± 0.022	1.46AsnPro: 1.46 ± 0.038	0.46AsnGln: 0.46 ± 0.022	1.142AsnArg: 1.142 ± 0.03	0.884AsnSer: 0.884 ± 0.03	1.092AsnThr: 1.092 ± 0.035	1.54AsnVal: 1.54 ± 0.037	0.259AsnTrp: 0.259 ± 0.013	0.418AsnTyr: 0.418 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	7.806ProAla: 7.806 ± 0.103	0.162ProCys: 0.162 ± 0.012	4.061ProAsp: 4.061 ± 0.072	3.63ProGlu: 3.63 ± 0.061	1.757ProPhe: 1.757 ± 0.034	5.074ProGly: 5.074 ± 0.077	1.182ProHis: 1.182 ± 0.033	2.184ProIle: 2.184 ± 0.049	0.924ProLys: 0.924 ± 0.031	4.804ProLeu: 4.804 ± 0.075	0.868ProMet: 0.868 ± 0.03	0.926ProAsn: 0.926 ± 0.031	2.397ProPro: 2.397 ± 0.059	1.484ProGln: 1.484 ± 0.035	3.53ProArg: 3.53 ± 0.07	2.931ProSer: 2.931 ± 0.054	3.576ProThr: 3.576 ± 0.075	5.006ProVal: 5.006 ± 0.06	0.911ProTrp: 0.911 ± 0.033	1.09ProTyr: 1.09 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.239GlnAla: 3.239 ± 0.055	0.099GlnCys: 0.099 ± 0.01	1.16GlnAsp: 1.16 ± 0.03	1.155GlnGlu: 1.155 ± 0.031	0.83GlnPhe: 0.83 ± 0.028	1.895GlnGly: 1.895 ± 0.047	0.607GlnHis: 0.607 ± 0.023	1.303GlnIle: 1.303 ± 0.036	0.546GlnLys: 0.546 ± 0.025	2.788GlnLeu: 2.788 ± 0.051	0.498GlnMet: 0.498 ± 0.023	0.594GlnAsn: 0.594 ± 0.025	1.477GlnPro: 1.477 ± 0.038	1.021GlnGln: 1.021 ± 0.032	2.256GlnArg: 2.256 ± 0.044	1.442GlnSer: 1.442 ± 0.033	1.599GlnThr: 1.599 ± 0.04	2.215GlnVal: 2.215 ± 0.036	0.472GlnTrp: 0.472 ± 0.02	0.582GlnTyr: 0.582 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	9.309ArgAla: 9.309 ± 0.133	0.29ArgCys: 0.29 ± 0.018	4.417ArgAsp: 4.417 ± 0.072	4.171ArgGlu: 4.171 ± 0.065	2.377ArgPhe: 2.377 ± 0.048	5.72ArgGly: 5.72 ± 0.077	1.635ArgHis: 1.635 ± 0.044	3.619ArgIle: 3.619 ± 0.058	1.162ArgLys: 1.162 ± 0.037	7.195ArgLeu: 7.195 ± 0.1	1.724ArgMet: 1.724 ± 0.039	1.211ArgAsn: 1.211 ± 0.031	3.592ArgPro: 3.592 ± 0.061	1.794ArgGln: 1.794 ± 0.045	6.699ArgArg: 6.699 ± 0.113	4.094ArgSer: 4.094 ± 0.06	4.239ArgThr: 4.239 ± 0.073	6.141ArgVal: 6.141 ± 0.079	1.232ArgTrp: 1.232 ± 0.032	1.569ArgTyr: 1.569 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	7.249SerAla: 7.249 ± 0.091	0.237SerCys: 0.237 ± 0.016	3.261SerAsp: 3.261 ± 0.058	2.52SerGlu: 2.52 ± 0.049	1.85SerPhe: 1.85 ± 0.042	5.619SerGly: 5.619 ± 0.089	0.994SerHis: 0.994 ± 0.031	2.617SerIle: 2.617 ± 0.05	0.892SerLys: 0.892 ± 0.031	5.236SerLeu: 5.236 ± 0.073	1.117SerMet: 1.117 ± 0.028	0.978SerAsn: 0.978 ± 0.033	3.099SerPro: 3.099 ± 0.05	1.242SerGln: 1.242 ± 0.034	3.618SerArg: 3.618 ± 0.056	3.211SerSer: 3.211 ± 0.06	3.624SerThr: 3.624 ± 0.065	4.822SerVal: 4.822 ± 0.079	0.963SerTrp: 0.963 ± 0.026	1.112SerTyr: 1.112 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	8.555ThrAla: 8.555 ± 0.119	0.281ThrCys: 0.281 ± 0.019	4.026ThrAsp: 4.026 ± 0.07	2.924ThrGlu: 2.924 ± 0.057	1.909ThrPhe: 1.909 ± 0.049	5.977ThrGly: 5.977 ± 0.085	1.142ThrHis: 1.142 ± 0.033	2.87ThrIle: 2.87 ± 0.063	1.028ThrLys: 1.028 ± 0.034	5.911ThrLeu: 5.911 ± 0.089	0.965ThrMet: 0.965 ± 0.028	1.08ThrAsn: 1.08 ± 0.036	3.947ThrPro: 3.947 ± 0.067	1.417ThrGln: 1.417 ± 0.035	3.765ThrArg: 3.765 ± 0.054	3.25ThrSer: 3.25 ± 0.066	3.943ThrThr: 3.943 ± 0.079	6.008ThrVal: 6.008 ± 0.096	0.991ThrTrp: 0.991 ± 0.028	1.244ThrTyr: 1.244 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	12.645ValAla: 12.645 ± 0.13	0.49ValCys: 0.49 ± 0.022	6.355ValAsp: 6.355 ± 0.08	5.032ValGlu: 5.032 ± 0.066	3.011ValPhe: 3.011 ± 0.048	7.438ValGly: 7.438 ± 0.091	1.714ValHis: 1.714 ± 0.035	4.422ValIle: 4.422 ± 0.068	1.482ValLys: 1.482 ± 0.038	9.17ValLeu: 9.17 ± 0.104	1.465ValMet: 1.465 ± 0.037	1.72ValAsn: 1.72 ± 0.044	4.782ValPro: 4.782 ± 0.066	2.187ValGln: 2.187 ± 0.047	6.227ValArg: 6.227 ± 0.08	5.126ValSer: 5.126 ± 0.08	5.831ValThr: 5.831 ± 0.103	9.53ValVal: 9.53 ± 0.111	1.338ValTrp: 1.338 ± 0.028	1.596ValTyr: 1.596 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.724TrpAla: 1.724 ± 0.039	0.102TrpCys: 0.102 ± 0.01	0.892TrpAsp: 0.892 ± 0.028	0.697TrpGlu: 0.697 ± 0.025	0.613TrpPhe: 0.613 ± 0.023	1.217TrpGly: 1.217 ± 0.032	0.368TrpHis: 0.368 ± 0.019	0.764TrpIle: 0.764 ± 0.026	0.268TrpLys: 0.268 ± 0.018	1.827TrpLeu: 1.827 ± 0.045	0.386TrpMet: 0.386 ± 0.018	0.451TrpAsn: 0.451 ± 0.019	0.78TrpPro: 0.78 ± 0.024	0.577TrpGln: 0.577 ± 0.023	1.305TrpArg: 1.305 ± 0.035	1.031TrpSer: 1.031 ± 0.033	1.007TrpThr: 1.007 ± 0.032	1.286TrpVal: 1.286 ± 0.035	0.413TrpTrp: 0.413 ± 0.018	0.326TrpTyr: 0.326 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.528TyrAla: 2.528 ± 0.044	0.102TyrCys: 0.102 ± 0.01	1.442TyrAsp: 1.442 ± 0.036	1.124TyrGlu: 1.124 ± 0.029	0.707TyrPhe: 0.707 ± 0.024	1.927TyrGly: 1.927 ± 0.042	0.304TyrHis: 0.304 ± 0.015	0.728TyrIle: 0.728 ± 0.027	0.316TyrLys: 0.316 ± 0.016	2.118TyrLeu: 2.118 ± 0.043	0.258TyrMet: 0.258 ± 0.015	0.46TyrAsn: 0.46 ± 0.021	1.033TyrPro: 1.033 ± 0.033	0.52TyrGln: 0.52 ± 0.025	1.646TyrArg: 1.646 ± 0.043	1.095TyrSer: 1.095 ± 0.032	1.162TyrThr: 1.162 ± 0.038	1.629TyrVal: 1.629 ± 0.043	0.336TyrTrp: 0.336 ± 0.018	0.483TyrTyr: 0.483 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3448 proteins (1191655 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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