Pseudogymnoascus verrucosus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10683 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B8GDJ5|A0A1B8GDJ5_9PEZI Zn(2)-C6 fungal-type domain-containing protein OS=Pseudogymnoascus verrucosus OX=342668 GN=VE01_08199 PE=4 SV=1
MM1 pKa = 7.54SGYY4 pKa = 11.32GNDD7 pKa = 3.77NNDD10 pKa = 3.5SYY12 pKa = 11.89GSSNTRR18 pKa = 11.84GGDD21 pKa = 3.47DD22 pKa = 4.52SYY24 pKa = 12.11GSSNNNSSNNDD35 pKa = 3.08SYY37 pKa = 11.9GSSNTRR43 pKa = 11.84SSDD46 pKa = 3.38NNDD49 pKa = 3.1SYY51 pKa = 11.93GSSNTRR57 pKa = 11.84SSDD60 pKa = 3.38NNDD63 pKa = 3.12SYY65 pKa = 12.05GSSNKK70 pKa = 9.68NSNDD74 pKa = 3.2DD75 pKa = 3.71DD76 pKa = 5.31SYY78 pKa = 11.7GSSNKK83 pKa = 9.9SSGNTDD89 pKa = 2.93SYY91 pKa = 11.57GSSGNSRR98 pKa = 11.84GDD100 pKa = 3.37NNDD103 pKa = 3.19SYY105 pKa = 11.95GSSNKK110 pKa = 9.82SSSNDD115 pKa = 2.74NDD117 pKa = 4.17TYY119 pKa = 11.56GSSNKK124 pKa = 9.56NSSDD128 pKa = 3.53TYY130 pKa = 11.12GSSNKK135 pKa = 9.55NSSDD139 pKa = 3.67SYY141 pKa = 10.85GSSGNTDD148 pKa = 2.99SYY150 pKa = 11.83GSSNKK155 pKa = 9.68NSNDD159 pKa = 3.04NDD161 pKa = 3.66DD162 pKa = 4.86SYY164 pKa = 12.21GSSNKK169 pKa = 9.79SSSNTDD175 pKa = 2.7SYY177 pKa = 11.94GSSNKK182 pKa = 9.58NSSDD186 pKa = 3.67SYY188 pKa = 10.85GSSGNTDD195 pKa = 2.99SYY197 pKa = 11.83GSSNKK202 pKa = 9.68NSNDD206 pKa = 3.2DD207 pKa = 3.71DD208 pKa = 5.25SYY210 pKa = 11.7GSSNTRR216 pKa = 11.84SSGNNNNSSDD226 pKa = 4.12SYY228 pKa = 11.61GSSNNNNNDD237 pKa = 3.11SYY239 pKa = 12.1GSGNNNSGSSTVDD252 pKa = 3.16KK253 pKa = 11.16LVDD256 pKa = 3.35TATGFLGRR264 pKa = 11.84SGGNNNNNDD273 pKa = 3.15SNYY276 pKa = 10.97

Molecular weight: 28.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B8GU12|A0A1B8GU12_9PEZI WD_REPEATS_REGION domain-containing protein OS=Pseudogymnoascus verrucosus OX=342668 GN=VE01_02574 PE=4 SV=1
MM1 pKa = 7.48SSSSPPRR8 pKa = 11.84DD9 pKa = 3.46PPPSQGGPPSIISSRR24 pKa = 11.84MTDD27 pKa = 2.8IASDD31 pKa = 4.04DD32 pKa = 3.74GHH34 pKa = 8.03DD35 pKa = 5.21LDD37 pKa = 4.67TTSHH41 pKa = 6.4ATPAHH46 pKa = 5.17RR47 pKa = 11.84TSTNRR52 pKa = 11.84QSIQSSRR59 pKa = 11.84PGTGSAAMSTRR70 pKa = 11.84SPWSASPPSRR80 pKa = 11.84RR81 pKa = 11.84GPKK84 pKa = 9.98SGGTPSILGGAAGARR99 pKa = 11.84PPSSTSRR106 pKa = 11.84THH108 pKa = 6.18VPSLTSHH115 pKa = 6.72AFFRR119 pKa = 11.84PMSSQRR125 pKa = 11.84LQAQRR130 pKa = 11.84GGPRR134 pKa = 11.84TPVAGQNQRR143 pKa = 11.84GSGDD147 pKa = 3.53FGDD150 pKa = 4.63APAVPSIASGYY161 pKa = 7.92TGNPIQSRR169 pKa = 11.84DD170 pKa = 3.52EE171 pKa = 4.39SGPPPPSRR179 pKa = 11.84GTEE182 pKa = 3.49ISVQEE187 pKa = 4.06YY188 pKa = 10.18VQTTTANTSPTHH200 pKa = 5.59GHH202 pKa = 5.87FQTTSLSSSVQPLQVNNASSNPNGLTVNTGLTTFRR237 pKa = 11.84NANLPTPSKK246 pKa = 10.14SPHH249 pKa = 5.26SFRR252 pKa = 11.84SSFLLPSRR260 pKa = 11.84GGDD263 pKa = 3.57MPPHH267 pKa = 6.11SPNRR271 pKa = 11.84SNHH274 pKa = 4.34GHH276 pKa = 6.06SKK278 pKa = 10.3LSSGATSPARR288 pKa = 11.84PSDD291 pKa = 3.83VTPLPSPLTPATGAMGEE308 pKa = 4.09KK309 pKa = 9.88HH310 pKa = 6.38GPGGNGGGGAGGGGKK325 pKa = 10.36GGVKK329 pKa = 10.25NYY331 pKa = 9.32VHH333 pKa = 6.97FPGNTRR339 pKa = 11.84FFLGGRR345 pKa = 11.84FQNARR350 pKa = 11.84DD351 pKa = 3.72RR352 pKa = 11.84PVNIATGILVVVPAVLFFVFQASWLWHH379 pKa = 5.36RR380 pKa = 11.84VSPAVPVVFAYY391 pKa = 10.78LSFICFSSFIHH402 pKa = 6.87ASVSDD407 pKa = 4.03PGILPRR413 pKa = 11.84DD414 pKa = 3.55LHH416 pKa = 6.56KK417 pKa = 10.79FPPPPATEE425 pKa = 4.85DD426 pKa = 4.11PLTLAPPTTAWLIVKK441 pKa = 9.43SHH443 pKa = 6.63LPASTAMEE451 pKa = 4.36VPVKK455 pKa = 10.49YY456 pKa = 10.6CKK458 pKa = 9.46TCHH461 pKa = 5.24IWRR464 pKa = 11.84PPRR467 pKa = 11.84GHH469 pKa = 7.0HH470 pKa = 7.04CRR472 pKa = 11.84ICDD475 pKa = 3.6NCIEE479 pKa = 4.44THH481 pKa = 6.28DD482 pKa = 4.18HH483 pKa = 5.9HH484 pKa = 7.48CVWLNNCVGRR494 pKa = 11.84RR495 pKa = 11.84NYY497 pKa = 10.45RR498 pKa = 11.84YY499 pKa = 9.85FFTFVAAGTGMAIFCIVTSVVQLSTVGRR527 pKa = 11.84DD528 pKa = 3.18NNSDD532 pKa = 3.56FGSAIPRR539 pKa = 11.84EE540 pKa = 4.06RR541 pKa = 11.84GVFALLIYY549 pKa = 10.38AALALPYY556 pKa = 9.73PAALLFYY563 pKa = 10.37HH564 pKa = 7.01IFLSGRR570 pKa = 11.84GEE572 pKa = 4.18TTRR575 pKa = 11.84EE576 pKa = 3.81LLNGRR581 pKa = 11.84KK582 pKa = 8.9FKK584 pKa = 10.62RR585 pKa = 11.84GEE587 pKa = 3.63RR588 pKa = 11.84HH589 pKa = 5.95RR590 pKa = 11.84PFTLGSVVKK599 pKa = 10.55NWIAVLGRR607 pKa = 11.84PRR609 pKa = 11.84GGGYY613 pKa = 9.66IGFKK617 pKa = 10.12RR618 pKa = 11.84VGGVWLGEE626 pKa = 4.01KK627 pKa = 10.41GVVEE631 pKa = 4.52GRR633 pKa = 11.84QGGSGGGNGEE643 pKa = 4.14MLEE646 pKa = 4.12MRR648 pKa = 11.84GMSQGAA654 pKa = 3.21

Molecular weight: 69.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10682

1

10683

5291759

51

5681

495.3

54.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.92 ± 0.023

1.194 ± 0.009

5.561 ± 0.02

6.269 ± 0.026

3.681 ± 0.015

7.342 ± 0.026

2.211 ± 0.01

5.061 ± 0.017

4.956 ± 0.023

8.74 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.205 ± 0.009

3.716 ± 0.012

5.957 ± 0.026

3.796 ± 0.02

5.81 ± 0.019

8.08 ± 0.024

6.081 ± 0.019

6.205 ± 0.017

1.433 ± 0.008

2.78 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski