Streptococcus pluranimalium
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1930 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L0D301|A0A2L0D301_9STRE Glycosyltransferase family 2 protein OS=Streptococcus pluranimalium OX=82348 GN=C0J00_03250 PE=4 SV=1
MM1 pKa = 7.57 ALAKK5 pKa = 10.1 VVYY8 pKa = 10.77 ASMTGNNEE16 pKa = 3.56 EE17 pKa = 4.35 LADD20 pKa = 4.26 IITNKK25 pKa = 10.33 LEE27 pKa = 4.31 EE28 pKa = 4.69 LGHH31 pKa = 4.63 TVEE34 pKa = 4.65 MEE36 pKa = 3.85 EE37 pKa = 4.26 CTMVEE42 pKa = 3.74 ASDD45 pKa = 5.57 FEE47 pKa = 4.68 DD48 pKa = 4.46 ADD50 pKa = 3.26 ICIVVTYY57 pKa = 9.46 TYY59 pKa = 11.24 DD60 pKa = 3.91 DD61 pKa = 4.35 GEE63 pKa = 4.85 LPDD66 pKa = 5.06 EE67 pKa = 4.34 IVDD70 pKa = 4.54 FYY72 pKa = 11.87 EE73 pKa = 4.22 EE74 pKa = 4.73 LADD77 pKa = 4.64 LDD79 pKa = 4.54 LSGKK83 pKa = 10.45 VYY85 pKa = 10.91 GCAGSGDD92 pKa = 3.63 TFYY95 pKa = 11.5 DD96 pKa = 4.01 DD97 pKa = 5.12 FCTCVDD103 pKa = 4.51 DD104 pKa = 4.96 FDD106 pKa = 4.66 KK107 pKa = 11.37 QFALTGATKK116 pKa = 10.2 GAEE119 pKa = 4.2 SVKK122 pKa = 10.36 VDD124 pKa = 4.67 LAADD128 pKa = 4.02 DD129 pKa = 4.43 EE130 pKa = 5.24 DD131 pKa = 4.23 IEE133 pKa = 4.2 NLEE136 pKa = 4.08 RR137 pKa = 11.84 FAEE140 pKa = 4.11 EE141 pKa = 4.62 LSACVSS147 pKa = 3.46
Molecular weight: 16.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.866
IPC2_protein 3.516
IPC_protein 3.516
Toseland 3.3
ProMoST 3.681
Dawson 3.503
Bjellqvist 3.656
Wikipedia 3.427
Rodwell 3.338
Grimsley 3.21
Solomon 3.49
Lehninger 3.439
Nozaki 3.617
DTASelect 3.821
Thurlkill 3.35
EMBOSS 3.439
Sillero 3.63
Patrickios 0.35
IPC_peptide 3.478
IPC2_peptide 3.605
IPC2.peptide.svr19 3.638
Protein with the highest isoelectric point:
>tr|A0A2L0D626|A0A2L0D626_9STRE Argininosuccinate synthase OS=Streptococcus pluranimalium OX=82348 GN=argG PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.2 GFRR19 pKa = 11.84 HH20 pKa = 6.39 RR21 pKa = 11.84 MSTKK25 pKa = 9.02 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1930
0
1930
580106
38
4378
300.6
33.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.582 ± 0.068
0.481 ± 0.014
6.017 ± 0.054
6.86 ± 0.056
4.54 ± 0.055
6.521 ± 0.055
1.911 ± 0.025
7.325 ± 0.049
6.793 ± 0.057
9.941 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.568 ± 0.029
4.46 ± 0.041
3.303 ± 0.037
4.041 ± 0.042
3.879 ± 0.043
6.386 ± 0.056
5.815 ± 0.064
6.977 ± 0.049
0.891 ± 0.023
3.71 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here