Amino acid dipepetide frequency for Streptococcus pluranimalium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.735AlaAla: 5.735 ± 0.156
0.457AlaCys: 0.457 ± 0.028
4.303AlaAsp: 4.303 ± 0.098
5.013AlaGlu: 5.013 ± 0.117
3.442AlaPhe: 3.442 ± 0.092
5.297AlaGly: 5.297 ± 0.12
1.281AlaHis: 1.281 ± 0.04
6.232AlaIle: 6.232 ± 0.129
5.589AlaLys: 5.589 ± 0.126
7.561AlaLeu: 7.561 ± 0.131
2.007AlaMet: 2.007 ± 0.062
3.21AlaAsn: 3.21 ± 0.081
2.032AlaPro: 2.032 ± 0.069
2.98AlaGln: 2.98 ± 0.077
2.834AlaArg: 2.834 ± 0.068
4.796AlaSer: 4.796 ± 0.106
4.308AlaThr: 4.308 ± 0.109
5.249AlaVal: 5.249 ± 0.118
0.615AlaTrp: 0.615 ± 0.032
2.879AlaTyr: 2.879 ± 0.081
0.0AlaXaa: 0.0 ± 0.0
Cys
0.265CysAla: 0.265 ± 0.023
0.055CysCys: 0.055 ± 0.011
0.309CysAsp: 0.309 ± 0.024
0.229CysGlu: 0.229 ± 0.02
0.291CysPhe: 0.291 ± 0.023
0.433CysGly: 0.433 ± 0.028
0.19CysHis: 0.19 ± 0.02
0.307CysIle: 0.307 ± 0.025
0.159CysLys: 0.159 ± 0.019
0.524CysLeu: 0.524 ± 0.03
0.112CysMet: 0.112 ± 0.012
0.133CysAsn: 0.133 ± 0.015
0.217CysPro: 0.217 ± 0.019
0.314CysGln: 0.314 ± 0.026
0.243CysArg: 0.243 ± 0.02
0.303CysSer: 0.303 ± 0.023
0.209CysThr: 0.209 ± 0.018
0.269CysVal: 0.269 ± 0.023
0.043CysTrp: 0.043 ± 0.01
0.207CysTyr: 0.207 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.858AspAla: 3.858 ± 0.104
0.279AspCys: 0.279 ± 0.025
3.48AspAsp: 3.48 ± 0.088
4.051AspGlu: 4.051 ± 0.092
3.487AspPhe: 3.487 ± 0.082
4.091AspGly: 4.091 ± 0.12
1.079AspHis: 1.079 ± 0.052
4.915AspIle: 4.915 ± 0.105
4.339AspLys: 4.339 ± 0.102
6.09AspLeu: 6.09 ± 0.109
1.72AspMet: 1.72 ± 0.048
2.82AspAsn: 2.82 ± 0.074
1.624AspPro: 1.624 ± 0.055
2.186AspGln: 2.186 ± 0.065
2.191AspArg: 2.191 ± 0.063
3.232AspSer: 3.232 ± 0.075
2.946AspThr: 2.946 ± 0.086
4.041AspVal: 4.041 ± 0.091
0.686AspTrp: 0.686 ± 0.037
3.058AspTyr: 3.058 ± 0.078
0.0AspXaa: 0.0 ± 0.0
Glu
5.871GluAla: 5.871 ± 0.125
0.269GluCys: 0.269 ± 0.023
4.042GluAsp: 4.042 ± 0.091
5.883GluGlu: 5.883 ± 0.13
2.463GluPhe: 2.463 ± 0.074
3.703GluGly: 3.703 ± 0.083
1.277GluHis: 1.277 ± 0.045
4.735GluIle: 4.735 ± 0.109
5.654GluLys: 5.654 ± 0.107
6.744GluLeu: 6.744 ± 0.128
1.808GluMet: 1.808 ± 0.062
3.741GluAsn: 3.741 ± 0.086
1.732GluPro: 1.732 ± 0.056
2.662GluGln: 2.662 ± 0.068
3.065GluArg: 3.065 ± 0.079
3.496GluSer: 3.496 ± 0.093
4.173GluThr: 4.173 ± 0.094
4.82GluVal: 4.82 ± 0.094
0.584GluTrp: 0.584 ± 0.028
1.874GluTyr: 1.874 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
3.168PheAla: 3.168 ± 0.082
0.212PheCys: 0.212 ± 0.02
3.299PheAsp: 3.299 ± 0.082
3.039PheGlu: 3.039 ± 0.083
2.143PhePhe: 2.143 ± 0.075
3.408PheGly: 3.408 ± 0.089
0.777PheHis: 0.777 ± 0.039
3.261PheIle: 3.261 ± 0.095
2.734PheLys: 2.734 ± 0.067
4.544PheLeu: 4.544 ± 0.121
1.146PheMet: 1.146 ± 0.046
1.934PheAsn: 1.934 ± 0.059
1.56PhePro: 1.56 ± 0.061
1.641PheGln: 1.641 ± 0.063
1.396PheArg: 1.396 ± 0.054
3.346PheSer: 3.346 ± 0.087
2.477PheThr: 2.477 ± 0.069
3.043PheVal: 3.043 ± 0.073
0.467PheTrp: 0.467 ± 0.028
1.801PheTyr: 1.801 ± 0.056
0.0PheXaa: 0.0 ± 0.0
Gly
4.589GlyAla: 4.589 ± 0.113
0.336GlyCys: 0.336 ± 0.022
3.575GlyAsp: 3.575 ± 0.09
3.551GlyGlu: 3.551 ± 0.072
3.399GlyPhe: 3.399 ± 0.085
4.208GlyGly: 4.208 ± 0.12
1.419GlyHis: 1.419 ± 0.054
5.523GlyIle: 5.523 ± 0.109
4.691GlyLys: 4.691 ± 0.098
6.692GlyLeu: 6.692 ± 0.129
1.776GlyMet: 1.776 ± 0.059
2.849GlyAsn: 2.849 ± 0.07
1.35GlyPro: 1.35 ± 0.051
2.996GlyGln: 2.996 ± 0.083
2.691GlyArg: 2.691 ± 0.066
3.81GlySer: 3.81 ± 0.086
3.684GlyThr: 3.684 ± 0.084
4.768GlyVal: 4.768 ± 0.102
0.676GlyTrp: 0.676 ± 0.038
2.624GlyTyr: 2.624 ± 0.074
0.0GlyXaa: 0.0 ± 0.0
His
1.155HisAla: 1.155 ± 0.048
0.115HisCys: 0.115 ± 0.014
1.12HisAsp: 1.12 ± 0.043
1.07HisGlu: 1.07 ± 0.042
1.146HisPhe: 1.146 ± 0.043
1.172HisGly: 1.172 ± 0.05
0.567HisHis: 0.567 ± 0.037
1.431HisIle: 1.431 ± 0.051
0.971HisLys: 0.971 ± 0.045
2.255HisLeu: 2.255 ± 0.066
0.453HisMet: 0.453 ± 0.027
0.707HisAsn: 0.707 ± 0.039
0.884HisPro: 0.884 ± 0.043
1.0HisGln: 1.0 ± 0.044
0.86HisArg: 0.86 ± 0.046
1.062HisSer: 1.062 ± 0.04
0.886HisThr: 0.886 ± 0.04
1.112HisVal: 1.112 ± 0.041
0.184HisTrp: 0.184 ± 0.019
0.953HisTyr: 0.953 ± 0.043
0.0HisXaa: 0.0 ± 0.0
Ile
6.106IleAla: 6.106 ± 0.114
0.503IleCys: 0.503 ± 0.028
4.672IleAsp: 4.672 ± 0.087
4.768IleGlu: 4.768 ± 0.11
3.299IlePhe: 3.299 ± 0.09
4.947IleGly: 4.947 ± 0.114
1.215IleHis: 1.215 ± 0.049
5.352IleIle: 5.352 ± 0.115
4.646IleLys: 4.646 ± 0.094
7.225IleLeu: 7.225 ± 0.156
1.798IleMet: 1.798 ± 0.063
3.291IleAsn: 3.291 ± 0.08
2.851IlePro: 2.851 ± 0.073
2.36IleGln: 2.36 ± 0.064
2.768IleArg: 2.768 ± 0.069
5.244IleSer: 5.244 ± 0.124
4.161IleThr: 4.161 ± 0.113
4.953IleVal: 4.953 ± 0.102
0.574IleTrp: 0.574 ± 0.033
2.513IleTyr: 2.513 ± 0.061
0.0IleXaa: 0.0 ± 0.0
Lys
5.513LysAla: 5.513 ± 0.123
0.212LysCys: 0.212 ± 0.018
4.291LysAsp: 4.291 ± 0.096
5.932LysGlu: 5.932 ± 0.106
1.884LysPhe: 1.884 ± 0.059
3.977LysGly: 3.977 ± 0.086
1.264LysHis: 1.264 ± 0.055
4.422LysIle: 4.422 ± 0.101
5.218LysLys: 5.218 ± 0.122
5.666LysLeu: 5.666 ± 0.095
2.031LysMet: 2.031 ± 0.057
3.396LysAsn: 3.396 ± 0.083
2.196LysPro: 2.196 ± 0.077
2.75LysGln: 2.75 ± 0.063
3.12LysArg: 3.12 ± 0.081
4.042LysSer: 4.042 ± 0.085
4.382LysThr: 4.382 ± 0.099
4.858LysVal: 4.858 ± 0.097
0.6LysTrp: 0.6 ± 0.035
2.175LysTyr: 2.175 ± 0.073
0.0LysXaa: 0.0 ± 0.0
Leu
8.769LeuAla: 8.769 ± 0.137
0.433LeuCys: 0.433 ± 0.03
5.904LeuAsp: 5.904 ± 0.124
7.107LeuGlu: 7.107 ± 0.144
4.246LeuPhe: 4.246 ± 0.119
6.556LeuGly: 6.556 ± 0.121
1.491LeuHis: 1.491 ± 0.053
6.69LeuIle: 6.69 ± 0.153
6.637LeuLys: 6.637 ± 0.113
9.765LeuLeu: 9.765 ± 0.198
2.558LeuMet: 2.558 ± 0.069
4.068LeuAsn: 4.068 ± 0.088
3.956LeuPro: 3.956 ± 0.077
3.275LeuGln: 3.275 ± 0.075
3.451LeuArg: 3.451 ± 0.093
7.585LeuSer: 7.585 ± 0.153
6.08LeuThr: 6.08 ± 0.106
7.056LeuVal: 7.056 ± 0.106
0.714LeuTrp: 0.714 ± 0.038
3.074LeuTyr: 3.074 ± 0.075
0.0LeuXaa: 0.0 ± 0.0
Met
2.219MetAla: 2.219 ± 0.067
0.1MetCys: 0.1 ± 0.013
1.364MetAsp: 1.364 ± 0.048
1.479MetGlu: 1.479 ± 0.05
0.891MetPhe: 0.891 ± 0.038
1.715MetGly: 1.715 ± 0.069
0.36MetHis: 0.36 ± 0.025
2.098MetIle: 2.098 ± 0.077
1.812MetLys: 1.812 ± 0.06
2.296MetLeu: 2.296 ± 0.07
0.793MetMet: 0.793 ± 0.042
1.102MetAsn: 1.102 ± 0.049
0.939MetPro: 0.939 ± 0.042
0.889MetGln: 0.889 ± 0.041
0.945MetArg: 0.945 ± 0.044
1.926MetSer: 1.926 ± 0.068
2.188MetThr: 2.188 ± 0.065
1.813MetVal: 1.813 ± 0.057
0.16MetTrp: 0.16 ± 0.015
0.591MetTyr: 0.591 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
3.056AsnAla: 3.056 ± 0.068
0.221AsnCys: 0.221 ± 0.02
2.679AsnAsp: 2.679 ± 0.068
2.432AsnGlu: 2.432 ± 0.07
1.863AsnPhe: 1.863 ± 0.057
3.239AsnGly: 3.239 ± 0.094
1.238AsnHis: 1.238 ± 0.05
3.258AsnIle: 3.258 ± 0.082
2.777AsnLys: 2.777 ± 0.095
4.403AsnLeu: 4.403 ± 0.086
1.11AsnMet: 1.11 ± 0.046
2.053AsnAsn: 2.053 ± 0.072
2.35AsnPro: 2.35 ± 0.067
2.429AsnGln: 2.429 ± 0.074
1.927AsnArg: 1.927 ± 0.059
2.312AsnSer: 2.312 ± 0.07
2.132AsnThr: 2.132 ± 0.069
2.774AsnVal: 2.774 ± 0.073
0.502AsnTrp: 0.502 ± 0.029
1.841AsnTyr: 1.841 ± 0.069
0.0AsnXaa: 0.0 ± 0.0
Pro
2.232ProAla: 2.232 ± 0.078
0.114ProCys: 0.114 ± 0.014
2.108ProAsp: 2.108 ± 0.055
2.948ProGlu: 2.948 ± 0.079
1.651ProPhe: 1.651 ± 0.054
1.838ProGly: 1.838 ± 0.06
0.653ProHis: 0.653 ± 0.034
2.427ProIle: 2.427 ± 0.066
2.363ProLys: 2.363 ± 0.066
3.074ProLeu: 3.074 ± 0.078
0.765ProMet: 0.765 ± 0.039
1.605ProAsn: 1.605 ± 0.057
0.646ProPro: 0.646 ± 0.096
1.277ProGln: 1.277 ± 0.044
1.027ProArg: 1.027 ± 0.043
2.053ProSer: 2.053 ± 0.06
2.096ProThr: 2.096 ± 0.084
2.608ProVal: 2.608 ± 0.082
0.281ProTrp: 0.281 ± 0.023
1.262ProTyr: 1.262 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
3.534GlnAla: 3.534 ± 0.101
0.124GlnCys: 0.124 ± 0.016
2.251GlnAsp: 2.251 ± 0.076
3.382GlnGlu: 3.382 ± 0.099
1.781GlnPhe: 1.781 ± 0.053
2.275GlnGly: 2.275 ± 0.07
0.698GlnHis: 0.698 ± 0.035
2.567GlnIle: 2.567 ± 0.061
3.041GlnLys: 3.041 ± 0.078
4.277GlnLeu: 4.277 ± 0.091
1.05GlnMet: 1.05 ± 0.044
1.855GlnAsn: 1.855 ± 0.044
1.146GlnPro: 1.146 ± 0.045
1.331GlnGln: 1.331 ± 0.054
1.374GlnArg: 1.374 ± 0.055
2.458GlnSer: 2.458 ± 0.067
2.351GlnThr: 2.351 ± 0.07
3.268GlnVal: 3.268 ± 0.079
0.305GlnTrp: 0.305 ± 0.024
1.339GlnTyr: 1.339 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
2.469ArgAla: 2.469 ± 0.071
0.176ArgCys: 0.176 ± 0.016
2.219ArgAsp: 2.219 ± 0.062
2.837ArgGlu: 2.837 ± 0.077
1.988ArgPhe: 1.988 ± 0.06
2.212ArgGly: 2.212 ± 0.07
0.936ArgHis: 0.936 ± 0.045
2.774ArgIle: 2.774 ± 0.062
2.608ArgLys: 2.608 ± 0.067
4.042ArgLeu: 4.042 ± 0.098
1.074ArgMet: 1.074 ± 0.048
1.579ArgAsn: 1.579 ± 0.044
1.255ArgPro: 1.255 ± 0.047
2.208ArgGln: 2.208 ± 0.06
1.922ArgArg: 1.922 ± 0.066
2.057ArgSer: 2.057 ± 0.063
1.879ArgThr: 1.879 ± 0.056
2.698ArgVal: 2.698 ± 0.076
0.3ArgTrp: 0.3 ± 0.025
1.558ArgTyr: 1.558 ± 0.056
0.0ArgXaa: 0.0 ± 0.0
Ser
4.189SerAla: 4.189 ± 0.107
0.262SerCys: 0.262 ± 0.023
3.761SerAsp: 3.761 ± 0.088
4.01SerGlu: 4.01 ± 0.11
3.186SerPhe: 3.186 ± 0.085
4.585SerGly: 4.585 ± 0.098
1.322SerHis: 1.322 ± 0.043
4.434SerIle: 4.434 ± 0.094
4.148SerLys: 4.148 ± 0.087
6.804SerLeu: 6.804 ± 0.139
1.489SerMet: 1.489 ± 0.055
2.555SerAsn: 2.555 ± 0.071
2.0SerPro: 2.0 ± 0.061
3.18SerGln: 3.18 ± 0.072
2.546SerArg: 2.546 ± 0.068
4.553SerSer: 4.553 ± 0.141
3.396SerThr: 3.396 ± 0.096
4.206SerVal: 4.206 ± 0.083
0.638SerTrp: 0.638 ± 0.032
2.596SerTyr: 2.596 ± 0.063
0.0SerXaa: 0.0 ± 0.0
Thr
4.263ThrAla: 4.263 ± 0.109
0.309ThrCys: 0.309 ± 0.026
3.406ThrAsp: 3.406 ± 0.085
3.589ThrGlu: 3.589 ± 0.088
2.825ThrPhe: 2.825 ± 0.072
4.127ThrGly: 4.127 ± 0.086
1.017ThrHis: 1.017 ± 0.039
4.701ThrIle: 4.701 ± 0.089
3.732ThrLys: 3.732 ± 0.075
5.609ThrLeu: 5.609 ± 0.093
1.195ThrMet: 1.195 ± 0.05
2.534ThrAsn: 2.534 ± 0.069
2.381ThrPro: 2.381 ± 0.129
1.903ThrGln: 1.903 ± 0.067
1.929ThrArg: 1.929 ± 0.064
3.817ThrSer: 3.817 ± 0.109
3.367ThrThr: 3.367 ± 0.114
4.463ThrVal: 4.463 ± 0.139
0.505ThrTrp: 0.505 ± 0.032
2.482ThrTyr: 2.482 ± 0.079
0.0ThrXaa: 0.0 ± 0.0
Val
5.861ValAla: 5.861 ± 0.138
0.365ValCys: 0.365 ± 0.025
4.437ValAsp: 4.437 ± 0.114
4.499ValGlu: 4.499 ± 0.106
3.018ValPhe: 3.018 ± 0.084
4.468ValGly: 4.468 ± 0.096
1.098ValHis: 1.098 ± 0.044
5.271ValIle: 5.271 ± 0.11
4.353ValLys: 4.353 ± 0.101
6.613ValLeu: 6.613 ± 0.127
1.763ValMet: 1.763 ± 0.057
3.087ValAsn: 3.087 ± 0.08
2.36ValPro: 2.36 ± 0.071
2.065ValGln: 2.065 ± 0.059
2.513ValArg: 2.513 ± 0.065
5.013ValSer: 5.013 ± 0.107
4.996ValThr: 4.996 ± 0.147
5.113ValVal: 5.113 ± 0.122
0.55ValTrp: 0.55 ± 0.028
2.324ValTyr: 2.324 ± 0.069
0.0ValXaa: 0.0 ± 0.0
Trp
0.545TrpAla: 0.545 ± 0.034
0.043TrpCys: 0.043 ± 0.008
0.465TrpAsp: 0.465 ± 0.026
0.59TrpGlu: 0.59 ± 0.03
0.453TrpPhe: 0.453 ± 0.029
0.584TrpGly: 0.584 ± 0.034
0.191TrpHis: 0.191 ± 0.02
0.596TrpIle: 0.596 ± 0.039
0.527TrpLys: 0.527 ± 0.032
1.112TrpLeu: 1.112 ± 0.049
0.257TrpMet: 0.257 ± 0.021
0.467TrpAsn: 0.467 ± 0.034
0.152TrpPro: 0.152 ± 0.015
0.546TrpGln: 0.546 ± 0.03
0.338TrpArg: 0.338 ± 0.027
0.619TrpSer: 0.619 ± 0.037
0.474TrpThr: 0.474 ± 0.028
0.51TrpVal: 0.51 ± 0.035
0.119TrpTrp: 0.119 ± 0.014
0.324TrpTyr: 0.324 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.422TyrAla: 2.422 ± 0.071
0.226TyrCys: 0.226 ± 0.019
2.487TyrAsp: 2.487 ± 0.067
2.188TyrGlu: 2.188 ± 0.067
1.939TyrPhe: 1.939 ± 0.07
2.37TyrGly: 2.37 ± 0.067
1.033TyrHis: 1.033 ± 0.045
2.253TyrIle: 2.253 ± 0.069
1.931TyrLys: 1.931 ± 0.062
4.12TyrLeu: 4.12 ± 0.105
0.772TyrMet: 0.772 ± 0.035
1.61TyrAsn: 1.61 ± 0.062
1.398TyrPro: 1.398 ± 0.052
2.415TyrGln: 2.415 ± 0.061
1.648TyrArg: 1.648 ± 0.062
2.138TyrSer: 2.138 ± 0.06
1.969TyrThr: 1.969 ± 0.061
2.148TyrVal: 2.148 ± 0.061
0.41TyrTrp: 0.41 ± 0.027
1.626TyrTyr: 1.626 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1930 proteins (580106 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski