Marinilabilia rubra
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4010 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U2BBP0|A0A2U2BBP0_9BACT Site-specific DNA-methyltransferase (adenine-specific) OS=Marinilabilia rubra OX=2162893 GN=DDZ16_06080 PE=3 SV=1
MM1 pKa = 7.12 QLSKK5 pKa = 9.41 TWWYY9 pKa = 11.01 FLMATIFVFTACDD22 pKa = 4.14 DD23 pKa = 4.59 DD24 pKa = 7.72 DD25 pKa = 7.24 DD26 pKa = 7.44 DD27 pKa = 6.2 EE28 pKa = 5.73 DD29 pKa = 6.27 LNGNWVEE36 pKa = 4.03 LGAFEE41 pKa = 5.67 GIPRR45 pKa = 11.84 TDD47 pKa = 2.65 AVAFVIGDD55 pKa = 3.55 YY56 pKa = 10.73 AYY58 pKa = 11.22 VGTGYY63 pKa = 10.65 NGEE66 pKa = 3.91 EE67 pKa = 4.23 DD68 pKa = 3.34 EE69 pKa = 4.75 RR70 pKa = 11.84 LRR72 pKa = 11.84 DD73 pKa = 3.91 FWRR76 pKa = 11.84 YY77 pKa = 9.89 DD78 pKa = 3.23 AVNDD82 pKa = 3.38 TWRR85 pKa = 11.84 EE86 pKa = 4.03 VASLPAEE93 pKa = 3.86 AAARR97 pKa = 11.84 NGAVAFSSNGKK108 pKa = 9.81 GYY110 pKa = 10.93 VGTGYY115 pKa = 10.79 DD116 pKa = 4.27 GEE118 pKa = 4.68 NKK120 pKa = 10.51 LKK122 pKa = 10.76 DD123 pKa = 3.56 FWEE126 pKa = 4.62 FNPAEE131 pKa = 4.6 GGDD134 pKa = 4.16 GQWTRR139 pKa = 11.84 IDD141 pKa = 4.68 DD142 pKa = 4.14 FPGTARR148 pKa = 11.84 YY149 pKa = 9.0 SAIAFGLEE157 pKa = 3.5 QNGYY161 pKa = 10.21 VGTGYY166 pKa = 10.82 DD167 pKa = 3.74 GNRR170 pKa = 11.84 LKK172 pKa = 11.0 DD173 pKa = 3.96 FYY175 pKa = 11.17 KK176 pKa = 10.69 YY177 pKa = 10.59 DD178 pKa = 3.78 AASGSWSQIMSIGGSKK194 pKa = 10.21 RR195 pKa = 11.84 RR196 pKa = 11.84 DD197 pKa = 3.09 AAVFVIDD204 pKa = 3.87 GKK206 pKa = 11.09 AYY208 pKa = 10.81 VFTGVDD214 pKa = 2.97 NSEE217 pKa = 4.36 YY218 pKa = 10.05 VTDD221 pKa = 3.06 AWMYY225 pKa = 11.01 DD226 pKa = 3.57 PQADD230 pKa = 3.12 TWTEE234 pKa = 3.59 KK235 pKa = 11.07 RR236 pKa = 11.84 EE237 pKa = 4.18 ITSGTNDD244 pKa = 3.75 DD245 pKa = 4.1 EE246 pKa = 6.43 SYY248 pKa = 11.78 DD249 pKa = 4.11 DD250 pKa = 5.36 DD251 pKa = 4.9 YY252 pKa = 12.16 SLAGTNVVAFSMGGLGYY269 pKa = 10.08 ISTGGQGYY277 pKa = 10.47 AGNTTWEE284 pKa = 4.35 YY285 pKa = 11.77 NPITDD290 pKa = 3.79 FWEE293 pKa = 4.36 EE294 pKa = 3.6 KK295 pKa = 10.82 SNFEE299 pKa = 4.03 GSSRR303 pKa = 11.84 SDD305 pKa = 2.86 AVAFAINNTPYY316 pKa = 10.67 VATGNSSGYY325 pKa = 10.63 YY326 pKa = 10.02 FDD328 pKa = 5.98 DD329 pKa = 2.86 VWTFYY334 pKa = 10.57 PDD336 pKa = 3.81 EE337 pKa = 4.31 EE338 pKa = 5.08 QNDD341 pKa = 3.38 NDD343 pKa = 3.73
Molecular weight: 38.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.605
ProMoST 3.961
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.503
Solomon 3.821
Lehninger 3.77
Nozaki 3.923
DTASelect 4.19
Thurlkill 3.656
EMBOSS 3.77
Sillero 3.948
Patrickios 1.341
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.835
Protein with the highest isoelectric point:
>tr|A0A2U2B8G7|A0A2U2B8G7_9BACT Uncharacterized protein OS=Marinilabilia rubra OX=2162893 GN=DDZ16_10130 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 KK11 pKa = 8.89 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.98 HH16 pKa = 3.99 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MTTVNGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.54 GRR39 pKa = 11.84 KK40 pKa = 8.81 RR41 pKa = 11.84 LTVSNEE47 pKa = 3.61 KK48 pKa = 9.93 RR49 pKa = 11.84 HH50 pKa = 5.47 KK51 pKa = 10.53 AA52 pKa = 3.32
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.459
IPC2_protein 10.994
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.457
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.179
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4010
0
4010
1511230
27
7413
376.9
42.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.309 ± 0.032
0.76 ± 0.012
5.755 ± 0.031
7.062 ± 0.036
5.159 ± 0.033
7.042 ± 0.043
1.946 ± 0.019
6.976 ± 0.036
6.746 ± 0.054
9.24 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.468 ± 0.022
5.457 ± 0.036
3.919 ± 0.021
3.41 ± 0.021
4.304 ± 0.029
6.684 ± 0.04
5.146 ± 0.049
6.462 ± 0.032
1.286 ± 0.015
3.868 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here