Streptococcus phage Javan336

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AZ32|A0A4D6AZ32_9CAUD Uncharacterized protein OS=Streptococcus phage Javan336 OX=2548112 GN=Javan336_0031 PE=4 SV=1
MM1 pKa = 8.04SYY3 pKa = 10.47EE4 pKa = 4.17QISEE8 pKa = 3.97STYY11 pKa = 9.4FQNMSYY17 pKa = 9.91WNQVAQNYY25 pKa = 7.71RR26 pKa = 11.84ALGGLGICDD35 pKa = 4.23DD36 pKa = 3.94EE37 pKa = 4.7TGEE40 pKa = 4.01EE41 pKa = 5.39LYY43 pKa = 10.59TII45 pKa = 4.75

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AZ11|A0A4D6AZ11_9CAUD Uncharacterized protein OS=Streptococcus phage Javan336 OX=2548112 GN=Javan336_0011 PE=4 SV=1
MM1 pKa = 7.59SKK3 pKa = 8.46ITLDD7 pKa = 3.27QAKK10 pKa = 9.37IDD12 pKa = 3.74MYY14 pKa = 11.33INLLKK19 pKa = 10.63RR20 pKa = 11.84GAIDD24 pKa = 3.68FSFVNKK30 pKa = 10.08RR31 pKa = 11.84FRR33 pKa = 11.84DD34 pKa = 3.6RR35 pKa = 11.84VRR37 pKa = 11.84KK38 pKa = 8.83EE39 pKa = 3.64LEE41 pKa = 3.72RR42 pKa = 11.84LGLSNLANN50 pKa = 3.69

Molecular weight: 5.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

12538

37

705

174.1

19.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.373 ± 0.45

0.646 ± 0.097

6.325 ± 0.279

8.048 ± 0.443

3.9 ± 0.197

6.452 ± 0.392

1.595 ± 0.166

6.771 ± 0.235

8.821 ± 0.396

8.359 ± 0.352

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.252

5.176 ± 0.242

2.632 ± 0.236

4.028 ± 0.182

4.602 ± 0.253

5.615 ± 0.27

5.982 ± 0.275

6.835 ± 0.294

1.3 ± 0.155

3.948 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski