Peptococcaceae bacterium CEB3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; unclassified Peptococcaceae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5055 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J1FI42|A0A0J1FI42_9FIRM 3'-5' exoribonuclease YhaM OS=Peptococcaceae bacterium CEB3 OX=999898 GN=yhaM_2 PE=4 SV=1
MM1 pKa = 7.64DD2 pKa = 4.41TLAFSDD8 pKa = 3.97EE9 pKa = 4.47DD10 pKa = 4.92EE11 pKa = 4.34IALDD15 pKa = 3.58YY16 pKa = 11.41KK17 pKa = 10.8LIQDD21 pKa = 4.51TYY23 pKa = 11.48DD24 pKa = 3.32TDD26 pKa = 3.68CYY28 pKa = 11.06NIYY31 pKa = 10.24FRR33 pKa = 11.84QEE35 pKa = 3.69VFNKK39 pKa = 9.5FLKK42 pKa = 10.76NISLAA47 pKa = 3.66

Molecular weight: 5.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J1FHQ7|A0A0J1FHQ7_9FIRM Transposase OS=Peptococcaceae bacterium CEB3 OX=999898 GN=CEB3_c06270 PE=4 SV=1
MM1 pKa = 7.05SRR3 pKa = 11.84RR4 pKa = 11.84WVSGLVAGGVAGAALGLLVLPRR26 pKa = 11.84RR27 pKa = 11.84TRR29 pKa = 11.84NSWTRR34 pKa = 11.84QGRR37 pKa = 11.84RR38 pKa = 11.84AWTRR42 pKa = 11.84GRR44 pKa = 11.84KK45 pKa = 8.49IWGG48 pKa = 3.36

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5055

0

5055

1468052

29

3009

290.4

32.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.167 ± 0.036

1.046 ± 0.017

4.627 ± 0.025

6.692 ± 0.047

3.916 ± 0.029

8.358 ± 0.032

1.892 ± 0.013

5.891 ± 0.029

5.016 ± 0.032

10.707 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.017

3.331 ± 0.024

4.58 ± 0.023

3.591 ± 0.02

6.007 ± 0.038

5.655 ± 0.025

5.087 ± 0.027

7.685 ± 0.028

1.237 ± 0.015

3.044 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski