Amino acid dipepetide frequency for Peptococcaceae bacterium CEB3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.846AlaAla: 9.846 ± 0.114	1.019AlaCys: 1.019 ± 0.029	4.244AlaAsp: 4.244 ± 0.06	6.569AlaGlu: 6.569 ± 0.074	3.31AlaPhe: 3.31 ± 0.046	8.821AlaGly: 8.821 ± 0.095	1.638AlaHis: 1.638 ± 0.035	4.824AlaIle: 4.824 ± 0.067	4.759AlaLys: 4.759 ± 0.066	10.338AlaLeu: 10.338 ± 0.098	2.321AlaMet: 2.321 ± 0.04	2.69AlaAsn: 2.69 ± 0.049	3.12AlaPro: 3.12 ± 0.054	3.629AlaGln: 3.629 ± 0.06	5.182AlaArg: 5.182 ± 0.065	4.474AlaSer: 4.474 ± 0.061	3.885AlaThr: 3.885 ± 0.056	7.308AlaVal: 7.308 ± 0.078	1.119AlaTrp: 1.119 ± 0.028	2.574AlaTyr: 2.574 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.847CysAla: 0.847 ± 0.028	0.161CysCys: 0.161 ± 0.012	0.492CysAsp: 0.492 ± 0.02	0.514CysGlu: 0.514 ± 0.019	0.351CysPhe: 0.351 ± 0.016	1.229CysGly: 1.229 ± 0.034	0.262CysHis: 0.262 ± 0.016	0.455CysIle: 0.455 ± 0.019	0.343CysLys: 0.343 ± 0.016	1.115CysLeu: 1.115 ± 0.029	0.21CysMet: 0.21 ± 0.012	0.315CysAsn: 0.315 ± 0.016	0.683CysPro: 0.683 ± 0.026	0.447CysGln: 0.447 ± 0.019	0.764CysArg: 0.764 ± 0.023	0.661CysSer: 0.661 ± 0.022	0.527CysThr: 0.527 ± 0.023	0.653CysVal: 0.653 ± 0.024	0.13CysTrp: 0.13 ± 0.011	0.303CysTyr: 0.303 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.543AspAla: 3.543 ± 0.056	0.484AspCys: 0.484 ± 0.019	1.803AspAsp: 1.803 ± 0.034	3.271AspGlu: 3.271 ± 0.058	2.195AspPhe: 2.195 ± 0.039	3.539AspGly: 3.539 ± 0.056	0.854AspHis: 0.854 ± 0.03	3.112AspIle: 3.112 ± 0.051	2.287AspLys: 2.287 ± 0.043	5.421AspLeu: 5.421 ± 0.066	1.243AspMet: 1.243 ± 0.031	1.353AspAsn: 1.353 ± 0.035	2.531AspPro: 2.531 ± 0.044	1.369AspGln: 1.369 ± 0.032	2.767AspArg: 2.767 ± 0.041	2.432AspSer: 2.432 ± 0.049	2.114AspThr: 2.114 ± 0.039	3.507AspVal: 3.507 ± 0.057	0.704AspTrp: 0.704 ± 0.023	1.738AspTyr: 1.738 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	6.03GluAla: 6.03 ± 0.081	0.55GluCys: 0.55 ± 0.021	2.943GluAsp: 2.943 ± 0.055	5.519GluGlu: 5.519 ± 0.088	2.313GluPhe: 2.313 ± 0.043	4.723GluGly: 4.723 ± 0.062	1.294GluHis: 1.294 ± 0.035	4.678GluIle: 4.678 ± 0.063	4.37GluLys: 4.37 ± 0.061	6.446GluLeu: 6.446 ± 0.084	1.828GluMet: 1.828 ± 0.04	2.509GluAsn: 2.509 ± 0.048	2.315GluPro: 2.315 ± 0.042	2.534GluGln: 2.534 ± 0.045	4.776GluArg: 4.776 ± 0.069	3.189GluSer: 3.189 ± 0.055	3.302GluThr: 3.302 ± 0.052	4.968GluVal: 4.968 ± 0.065	0.753GluTrp: 0.753 ± 0.024	1.877GluTyr: 1.877 ± 0.042	0.0GluXaa: 0.0 ± 0.0
Phe	3.579PheAla: 3.579 ± 0.047	0.492PheCys: 0.492 ± 0.019	1.862PheAsp: 1.862 ± 0.037	1.928PheGlu: 1.928 ± 0.035	1.815PhePhe: 1.815 ± 0.04	3.322PheGly: 3.322 ± 0.051	0.76PheHis: 0.76 ± 0.022	2.167PheIle: 2.167 ± 0.041	1.549PheLys: 1.549 ± 0.035	4.414PheLeu: 4.414 ± 0.071	0.94PheMet: 0.94 ± 0.029	1.362PheAsn: 1.362 ± 0.031	1.857PhePro: 1.857 ± 0.037	1.283PheGln: 1.283 ± 0.025	2.257PheArg: 2.257 ± 0.039	2.65PheSer: 2.65 ± 0.042	2.071PheThr: 2.071 ± 0.044	2.94PheVal: 2.94 ± 0.051	0.591PheTrp: 0.591 ± 0.022	1.326PheTyr: 1.326 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	6.675GlyAla: 6.675 ± 0.088	1.072GlyCys: 1.072 ± 0.033	3.428GlyAsp: 3.428 ± 0.043	5.303GlyGlu: 5.303 ± 0.067	3.417GlyPhe: 3.417 ± 0.055	6.708GlyGly: 6.708 ± 0.085	1.548GlyHis: 1.548 ± 0.033	5.843GlyIle: 5.843 ± 0.067	4.868GlyLys: 4.868 ± 0.061	8.919GlyLeu: 8.919 ± 0.078	2.405GlyMet: 2.405 ± 0.041	2.699GlyAsn: 2.699 ± 0.04	2.76GlyPro: 2.76 ± 0.053	3.201GlyGln: 3.201 ± 0.051	4.951GlyArg: 4.951 ± 0.072	4.774GlySer: 4.774 ± 0.068	4.605GlyThr: 4.605 ± 0.076	6.434GlyVal: 6.434 ± 0.066	1.134GlyTrp: 1.134 ± 0.029	2.84GlyTyr: 2.84 ± 0.047	0.001GlyXaa: 0.001 ± 0.001
His	1.287HisAla: 1.287 ± 0.03	0.298HisCys: 0.298 ± 0.013	0.91HisAsp: 0.91 ± 0.03	0.973HisGlu: 0.973 ± 0.024	0.842HisPhe: 0.842 ± 0.026	1.539HisGly: 1.539 ± 0.03	0.44HisHis: 0.44 ± 0.019	1.061HisIle: 1.061 ± 0.03	0.789HisLys: 0.789 ± 0.021	2.175HisLeu: 2.175 ± 0.039	0.463HisMet: 0.463 ± 0.017	0.653HisAsn: 0.653 ± 0.021	1.361HisPro: 1.361 ± 0.028	0.604HisGln: 0.604 ± 0.022	1.136HisArg: 1.136 ± 0.031	1.067HisSer: 1.067 ± 0.03	0.991HisThr: 0.991 ± 0.03	1.279HisVal: 1.279 ± 0.034	0.311HisTrp: 0.311 ± 0.015	0.743HisTyr: 0.743 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	5.323IleAla: 5.323 ± 0.058	0.645IleCys: 0.645 ± 0.021	2.835IleAsp: 2.835 ± 0.049	3.6IleGlu: 3.6 ± 0.055	2.341IlePhe: 2.341 ± 0.045	4.678IleGly: 4.678 ± 0.064	1.17IleHis: 1.17 ± 0.028	3.427IleIle: 3.427 ± 0.056	2.755IleLys: 2.755 ± 0.048	6.691IleLeu: 6.691 ± 0.076	1.418IleMet: 1.418 ± 0.033	1.992IleAsn: 1.992 ± 0.038	3.222IlePro: 3.222 ± 0.046	1.902IleGln: 1.902 ± 0.034	3.445IleArg: 3.445 ± 0.046	3.667IleSer: 3.667 ± 0.059	2.996IleThr: 2.996 ± 0.051	4.469IleVal: 4.469 ± 0.063	0.608IleTrp: 0.608 ± 0.021	1.727IleTyr: 1.727 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.714LysAla: 4.714 ± 0.063	0.403LysCys: 0.403 ± 0.018	2.484LysAsp: 2.484 ± 0.044	3.986LysGlu: 3.986 ± 0.055	1.506LysPhe: 1.506 ± 0.035	3.809LysGly: 3.809 ± 0.057	0.864LysHis: 0.864 ± 0.025	3.249LysIle: 3.249 ± 0.041	3.071LysLys: 3.071 ± 0.059	4.443LysLeu: 4.443 ± 0.062	1.341LysMet: 1.341 ± 0.033	1.898LysAsn: 1.898 ± 0.038	2.106LysPro: 2.106 ± 0.038	1.661LysGln: 1.661 ± 0.031	2.937LysArg: 2.937 ± 0.044	2.648LysSer: 2.648 ± 0.043	2.827LysThr: 2.827 ± 0.043	4.057LysVal: 4.057 ± 0.059	0.546LysTrp: 0.546 ± 0.022	1.612LysTyr: 1.612 ± 0.033	0.001LysXaa: 0.001 ± 0.001
Leu	11.394LeuAla: 11.394 ± 0.12	1.029LeuCys: 1.029 ± 0.03	5.063LeuAsp: 5.063 ± 0.063	6.699LeuGlu: 6.699 ± 0.096	3.961LeuPhe: 3.961 ± 0.07	8.782LeuGly: 8.782 ± 0.082	1.89LeuHis: 1.89 ± 0.038	5.722LeuIle: 5.722 ± 0.079	5.367LeuLys: 5.367 ± 0.07	10.956LeuLeu: 10.956 ± 0.111	2.42LeuMet: 2.42 ± 0.04	3.734LeuAsn: 3.734 ± 0.05	5.339LeuPro: 5.339 ± 0.065	3.516LeuGln: 3.516 ± 0.05	6.603LeuArg: 6.603 ± 0.08	6.945LeuSer: 6.945 ± 0.072	6.039LeuThr: 6.039 ± 0.067	7.629LeuVal: 7.629 ± 0.071	1.236LeuTrp: 1.236 ± 0.032	2.743LeuTyr: 2.743 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.629MetAla: 2.629 ± 0.043	0.188MetCys: 0.188 ± 0.01	1.261MetAsp: 1.261 ± 0.029	1.568MetGlu: 1.568 ± 0.033	0.89MetPhe: 0.89 ± 0.028	2.071MetGly: 2.071 ± 0.037	0.407MetHis: 0.407 ± 0.017	1.475MetIle: 1.475 ± 0.03	1.401MetLys: 1.401 ± 0.035	2.285MetLeu: 2.285 ± 0.042	0.609MetMet: 0.609 ± 0.02	0.964MetAsn: 0.964 ± 0.024	1.161MetPro: 1.161 ± 0.027	0.806MetGln: 0.806 ± 0.024	1.429MetArg: 1.429 ± 0.032	1.629MetSer: 1.629 ± 0.032	1.341MetThr: 1.341 ± 0.026	1.813MetVal: 1.813 ± 0.037	0.201MetTrp: 0.201 ± 0.011	0.574MetTyr: 0.574 ± 0.021	0.001MetXaa: 0.001 ± 0.001
Asn	2.59AsnAla: 2.59 ± 0.05	0.402AsnCys: 0.402 ± 0.018	1.334AsnAsp: 1.334 ± 0.03	1.715AsnGlu: 1.715 ± 0.037	1.339AsnPhe: 1.339 ± 0.031	2.727AsnGly: 2.727 ± 0.049	0.679AsnHis: 0.679 ± 0.023	2.223AsnIle: 2.223 ± 0.04	1.501AsnLys: 1.501 ± 0.032	3.661AsnLeu: 3.661 ± 0.048	0.859AsnMet: 0.859 ± 0.023	1.151AsnAsn: 1.151 ± 0.035	2.287AsnPro: 2.287 ± 0.041	1.175AsnGln: 1.175 ± 0.029	2.005AsnArg: 2.005 ± 0.038	1.881AsnSer: 1.881 ± 0.036	1.704AsnThr: 1.704 ± 0.038	2.461AsnVal: 2.461 ± 0.046	0.446AsnTrp: 0.446 ± 0.019	1.176AsnTyr: 1.176 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	4.32ProAla: 4.32 ± 0.065	0.388ProCys: 0.388 ± 0.018	2.618ProAsp: 2.618 ± 0.044	3.892ProGlu: 3.892 ± 0.057	1.871ProPhe: 1.871 ± 0.039	4.215ProGly: 4.215 ± 0.056	0.842ProHis: 0.842 ± 0.024	2.173ProIle: 2.173 ± 0.038	1.976ProLys: 1.976 ± 0.037	4.666ProLeu: 4.666 ± 0.061	0.929ProMet: 0.929 ± 0.024	1.42ProAsn: 1.42 ± 0.032	1.86ProPro: 1.86 ± 0.047	1.77ProGln: 1.77 ± 0.036	2.185ProArg: 2.185 ± 0.044	2.573ProSer: 2.573 ± 0.049	2.08ProThr: 2.08 ± 0.038	3.877ProVal: 3.877 ± 0.052	0.687ProTrp: 0.687 ± 0.027	1.454ProTyr: 1.454 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	4.028GlnAla: 4.028 ± 0.069	0.302GlnCys: 0.302 ± 0.015	1.632GlnAsp: 1.632 ± 0.031	2.697GlnGlu: 2.697 ± 0.049	1.142GlnPhe: 1.142 ± 0.03	3.103GlnGly: 3.103 ± 0.049	0.646GlnHis: 0.646 ± 0.02	2.18GlnIle: 2.18 ± 0.036	1.979GlnLys: 1.979 ± 0.036	2.989GlnLeu: 2.989 ± 0.048	0.936GlnMet: 0.936 ± 0.024	1.266GlnAsn: 1.266 ± 0.03	1.454GlnPro: 1.454 ± 0.031	1.27GlnGln: 1.27 ± 0.037	2.056GlnArg: 2.056 ± 0.041	1.834GlnSer: 1.834 ± 0.042	2.017GlnThr: 2.017 ± 0.041	2.975GlnVal: 2.975 ± 0.048	0.422GlnTrp: 0.422 ± 0.017	0.98GlnTyr: 0.98 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	4.729ArgAla: 4.729 ± 0.067	0.608ArgCys: 0.608 ± 0.022	2.825ArgAsp: 2.825 ± 0.049	5.112ArgGlu: 5.112 ± 0.071	2.375ArgPhe: 2.375 ± 0.044	4.286ArgGly: 4.286 ± 0.068	1.194ArgHis: 1.194 ± 0.032	3.533ArgIle: 3.533 ± 0.054	3.145ArgLys: 3.145 ± 0.051	6.673ArgLeu: 6.673 ± 0.08	1.5ArgMet: 1.5 ± 0.029	1.866ArgAsn: 1.866 ± 0.036	2.482ArgPro: 2.482 ± 0.038	2.638ArgGln: 2.638 ± 0.044	4.209ArgArg: 4.209 ± 0.062	2.916ArgSer: 2.916 ± 0.045	2.8ArgThr: 2.8 ± 0.047	4.571ArgVal: 4.571 ± 0.061	0.738ArgTrp: 0.738 ± 0.027	1.87ArgTyr: 1.87 ± 0.036	0.001ArgXaa: 0.001 ± 0.001
Ser	5.028SerAla: 5.028 ± 0.069	0.597SerCys: 0.597 ± 0.024	2.436SerAsp: 2.436 ± 0.045	3.284SerGlu: 3.284 ± 0.052	2.427SerPhe: 2.427 ± 0.049	5.691SerGly: 5.691 ± 0.076	1.119SerHis: 1.119 ± 0.03	2.979SerIle: 2.979 ± 0.051	2.364SerLys: 2.364 ± 0.042	6.532SerLeu: 6.532 ± 0.073	1.362SerMet: 1.362 ± 0.032	1.61SerAsn: 1.61 ± 0.035	2.797SerPro: 2.797 ± 0.046	2.114SerGln: 2.114 ± 0.045	3.293SerArg: 3.293 ± 0.042	3.405SerSer: 3.405 ± 0.057	2.743SerThr: 2.743 ± 0.05	4.285SerVal: 4.285 ± 0.055	0.745SerTrp: 0.745 ± 0.023	1.74SerTyr: 1.74 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	4.81ThrAla: 4.81 ± 0.078	0.512ThrCys: 0.512 ± 0.02	2.382ThrAsp: 2.382 ± 0.042	3.043ThrGlu: 3.043 ± 0.044	1.953ThrPhe: 1.953 ± 0.036	4.987ThrGly: 4.987 ± 0.06	0.982ThrHis: 0.982 ± 0.027	2.819ThrIle: 2.819 ± 0.046	2.071ThrLys: 2.071 ± 0.04	5.699ThrLeu: 5.699 ± 0.066	1.196ThrMet: 1.196 ± 0.029	1.593ThrAsn: 1.593 ± 0.036	2.695ThrPro: 2.695 ± 0.048	1.623ThrGln: 1.623 ± 0.032	2.47ThrArg: 2.47 ± 0.038	2.67ThrSer: 2.67 ± 0.05	2.626ThrThr: 2.626 ± 0.047	4.63ThrVal: 4.63 ± 0.068	0.601ThrTrp: 0.601 ± 0.024	1.506ThrTyr: 1.506 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	6.62ValAla: 6.62 ± 0.068	0.87ValCys: 0.87 ± 0.023	3.645ValAsp: 3.645 ± 0.057	4.74ValGlu: 4.74 ± 0.067	3.221ValPhe: 3.221 ± 0.047	5.837ValGly: 5.837 ± 0.071	1.382ValHis: 1.382 ± 0.029	4.751ValIle: 4.751 ± 0.068	3.778ValLys: 3.778 ± 0.053	8.628ValLeu: 8.628 ± 0.09	1.873ValMet: 1.873 ± 0.037	2.779ValAsn: 2.779 ± 0.045	3.609ValPro: 3.609 ± 0.052	2.528ValGln: 2.528 ± 0.042	4.723ValArg: 4.723 ± 0.065	4.728ValSer: 4.728 ± 0.061	4.122ValThr: 4.122 ± 0.056	6.021ValVal: 6.021 ± 0.071	0.83ValTrp: 0.83 ± 0.029	2.164ValTyr: 2.164 ± 0.038	0.001ValXaa: 0.001 ± 0.001
Trp	1.03TrpAla: 1.03 ± 0.028	0.088TrpCys: 0.088 ± 0.008	0.563TrpAsp: 0.563 ± 0.018	0.775TrpGlu: 0.775 ± 0.022	0.467TrpPhe: 0.467 ± 0.021	0.958TrpGly: 0.958 ± 0.03	0.274TrpHis: 0.274 ± 0.015	0.655TrpIle: 0.655 ± 0.02	0.536TrpLys: 0.536 ± 0.019	1.544TrpLeu: 1.544 ± 0.037	0.302TrpMet: 0.302 ± 0.016	0.464TrpAsn: 0.464 ± 0.016	0.546TrpPro: 0.546 ± 0.019	0.715TrpGln: 0.715 ± 0.023	0.811TrpArg: 0.811 ± 0.029	0.71TrpSer: 0.71 ± 0.023	0.554TrpThr: 0.554 ± 0.02	0.879TrpVal: 0.879 ± 0.025	0.166TrpTrp: 0.166 ± 0.01	0.337TrpTyr: 0.337 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.647TyrAla: 2.647 ± 0.05	0.354TyrCys: 0.354 ± 0.018	1.507TyrAsp: 1.507 ± 0.036	1.727TyrGlu: 1.727 ± 0.038	1.428TyrPhe: 1.428 ± 0.031	2.561TyrGly: 2.561 ± 0.04	0.676TyrHis: 0.676 ± 0.024	1.586TyrIle: 1.586 ± 0.034	1.251TyrLys: 1.251 ± 0.034	3.473TyrLeu: 3.473 ± 0.052	0.547TyrMet: 0.547 ± 0.018	0.997TyrAsn: 0.997 ± 0.028	1.612TyrPro: 1.612 ± 0.036	1.124TyrGln: 1.124 ± 0.026	2.071TyrArg: 2.071 ± 0.039	1.7TyrSer: 1.7 ± 0.033	1.525TyrThr: 1.525 ± 0.035	2.095TyrVal: 2.095 ± 0.042	0.404TyrTrp: 0.404 ± 0.016	1.152TyrTyr: 1.152 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5055 proteins (1468052 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---