Streptomyces sp. TverLS-915
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6283 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1C4NDN9|A0A1C4NDN9_9ACTN Transcriptional regulator TetR family OS=Streptomyces sp. TverLS-915 OX=1839763 GN=GA0115251_131118 PE=4 SV=1
MM1 pKa = 7.62 SNPTNLRR8 pKa = 11.84 YY9 pKa = 10.16 SKK11 pKa = 9.51 EE12 pKa = 4.21 HH13 pKa = 5.94 EE14 pKa = 4.35 WLSAAADD21 pKa = 3.98 GVSTVGITAYY31 pKa = 10.47 AAGQLGDD38 pKa = 3.59 VVYY41 pKa = 10.63 AQLPEE46 pKa = 4.24 VGDD49 pKa = 3.85 SVEE52 pKa = 4.29 AGEE55 pKa = 4.55 TCGEE59 pKa = 4.12 LEE61 pKa = 4.31 STKK64 pKa = 10.61 SVSDD68 pKa = 4.01 LYY70 pKa = 11.51 SPVNGEE76 pKa = 3.99 VTEE79 pKa = 4.31 VNSDD83 pKa = 3.63 VVEE86 pKa = 4.72 DD87 pKa = 4.11 PSLVNSAPFEE97 pKa = 4.42 GGWLFKK103 pKa = 11.02 VRR105 pKa = 11.84 VSEE108 pKa = 4.21 EE109 pKa = 3.99 PGDD112 pKa = 3.81 LLSAEE117 pKa = 4.62 EE118 pKa = 4.07 YY119 pKa = 10.95 AAFAGSDD126 pKa = 3.58 DD127 pKa = 3.64
Molecular weight: 13.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.783
IPC_protein 3.706
Toseland 3.528
ProMoST 3.795
Dawson 3.681
Bjellqvist 3.884
Wikipedia 3.579
Rodwell 3.541
Grimsley 3.439
Solomon 3.656
Lehninger 3.617
Nozaki 3.795
DTASelect 3.935
Thurlkill 3.567
EMBOSS 3.592
Sillero 3.821
Patrickios 1.825
IPC_peptide 3.656
IPC2_peptide 3.795
IPC2.peptide.svr19 3.777
Protein with the highest isoelectric point:
>tr|A0A1C4K5M3|A0A1C4K5M3_9ACTN Putative efflux protein MATE family OS=Streptomyces sp. TverLS-915 OX=1839763 GN=GA0115251_112542 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILASRR35 pKa = 11.84 RR36 pKa = 11.84 NKK38 pKa = 9.46 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSAA45 pKa = 3.91
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6283
0
6283
2005380
24
6338
319.2
34.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.231 ± 0.05
0.729 ± 0.008
5.703 ± 0.026
5.862 ± 0.037
2.641 ± 0.015
9.909 ± 0.034
2.321 ± 0.017
2.664 ± 0.019
2.11 ± 0.03
10.547 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.532 ± 0.012
1.614 ± 0.017
6.568 ± 0.032
2.407 ± 0.018
8.364 ± 0.04
4.862 ± 0.022
6.097 ± 0.029
8.306 ± 0.032
1.487 ± 0.012
2.045 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here