Pseudomonas jinjuensis
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5169 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H0BER0|A0A1H0BER0_9PSED Uncharacterized protein OS=Pseudomonas jinjuensis OX=198616 GN=SAMN05216193_10342 PE=4 SV=1
MM1 pKa = 7.48 FKK3 pKa = 10.77 LKK5 pKa = 10.14 PLAIAILTLASAQVMAAGNTATQIQLSGDD34 pKa = 4.12 GNDD37 pKa = 3.41 STAMQTGISDD47 pKa = 4.27 GNTSTQVQAGDD58 pKa = 3.48 NNTANVRR65 pKa = 11.84 QLDD68 pKa = 3.77 VSTGNVAGQAQAGNGNTATIEE89 pKa = 4.35 QKK91 pKa = 9.63 WGAYY95 pKa = 8.76 YY96 pKa = 10.94 NEE98 pKa = 4.33 AAQAQTGNGNTATVDD113 pKa = 3.34 QHH115 pKa = 8.43 DD116 pKa = 4.42 EE117 pKa = 3.73 ATANHH122 pKa = 6.56 VYY124 pKa = 9.94 QVQDD128 pKa = 3.26 GSANKK133 pKa = 10.14 AFAEE137 pKa = 4.02 NAYY140 pKa = 10.6 VSTGNYY146 pKa = 7.38 ATQLQSGTSHH156 pKa = 6.4 EE157 pKa = 4.11 AEE159 pKa = 4.34 IWQQWGEE166 pKa = 4.21 GNQALQVQDD175 pKa = 4.63 GDD177 pKa = 3.54 DD178 pKa = 4.24 HH179 pKa = 5.83 YY180 pKa = 12.14 AKK182 pKa = 10.41 VYY184 pKa = 10.42 QGADD188 pKa = 3.15 ANSNISVQTQTGTSNTSTVNQLFSADD214 pKa = 3.49 GNYY217 pKa = 10.3 SEE219 pKa = 4.63 VTQNGVGAGNSALVEE234 pKa = 4.24 QNWGGGNVSVLDD246 pKa = 3.71 QDD248 pKa = 4.59 GDD250 pKa = 3.97 GNDD253 pKa = 3.11 AHH255 pKa = 6.48 VTQQHH260 pKa = 6.12 GDD262 pKa = 3.63 GNGSALTQAGNGNLASVSQHH282 pKa = 6.96 DD283 pKa = 3.85 GDD285 pKa = 4.72 GNQSALTQTGNYY297 pKa = 9.26 NSASVTQADD306 pKa = 4.04 GGWNVSSLSQSGNNHH321 pKa = 5.5 QATVTQGGGNSNEE334 pKa = 4.63 LYY336 pKa = 10.67 FEE338 pKa = 4.07 QDD340 pKa = 2.71 DD341 pKa = 4.39 DD342 pKa = 5.7 GNVLTADD349 pKa = 3.85 QSGTDD354 pKa = 3.68 NLITGYY360 pKa = 9.97 SHH362 pKa = 7.57 GDD364 pKa = 3.55 DD365 pKa = 3.3 NVVDD369 pKa = 3.92 VDD371 pKa = 4.38 QVGSLNTAHH380 pKa = 6.42 VEE382 pKa = 4.17 QYY384 pKa = 10.74 SGSSEE389 pKa = 3.87 NLADD393 pKa = 3.83 ISQTGSDD400 pKa = 3.35 HH401 pKa = 6.35 VATVLQGGMGNEE413 pKa = 3.84 AFVTQTSTGNTAYY426 pKa = 10.8 VLQAGTNGTATIIQNN441 pKa = 3.44
Molecular weight: 45.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.745
IPC_protein 3.783
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.91
Patrickios 0.477
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.809
Protein with the highest isoelectric point:
>tr|A0A1H0JA05|A0A1H0JA05_9PSED Uncharacterized protein YydD contains DUF2326 domain OS=Pseudomonas jinjuensis OX=198616 GN=SAMN05216193_1114 PE=4 SV=1
MM1 pKa = 7.55 PRR3 pKa = 11.84 YY4 pKa = 10.08 ALITGASSGIGLALAEE20 pKa = 4.06 ALARR24 pKa = 11.84 RR25 pKa = 11.84 GQALVLVARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 DD37 pKa = 3.63 ALEE40 pKa = 4.64 SIACEE45 pKa = 3.49 LAQRR49 pKa = 11.84 FGVDD53 pKa = 3.18 VLFRR57 pKa = 11.84 TCDD60 pKa = 3.28 LSEE63 pKa = 4.27 PLQISGLLHH72 pKa = 6.66 EE73 pKa = 5.77 LDD75 pKa = 3.99 QNGLDD80 pKa = 4.14 IEE82 pKa = 4.63 LLVNSAGIGSAGAFLEE98 pKa = 4.56 HH99 pKa = 6.95 DD100 pKa = 3.45 WSRR103 pKa = 11.84 EE104 pKa = 3.81 RR105 pKa = 11.84 EE106 pKa = 4.18 LVEE109 pKa = 4.84 LNVLALTRR117 pKa = 11.84 LCHH120 pKa = 7.24 AIGNRR125 pKa = 11.84 MAQNGGGRR133 pKa = 11.84 ILNVASLAALHH144 pKa = 6.49 PGPWMSSYY152 pKa = 10.19 HH153 pKa = 5.95 ASKK156 pKa = 10.72 AYY158 pKa = 10.08 VLSFSEE164 pKa = 4.4 GLRR167 pKa = 11.84 EE168 pKa = 3.89 EE169 pKa = 4.23 LKK171 pKa = 10.79 GRR173 pKa = 11.84 GVKK176 pKa = 10.31 VSLLCPGPTRR186 pKa = 11.84 SAFFRR191 pKa = 11.84 HH192 pKa = 6.13 AGLDD196 pKa = 3.55 GEE198 pKa = 4.37 RR199 pKa = 11.84 FEE201 pKa = 4.77 GKK203 pKa = 10.28 RR204 pKa = 11.84 MLSPEE209 pKa = 3.64 QVAFHH214 pKa = 5.94 TVRR217 pKa = 11.84 ALQRR221 pKa = 11.84 NPAIIIPGWRR231 pKa = 11.84 NRR233 pKa = 11.84 LLAWCPRR240 pKa = 11.84 LAPRR244 pKa = 11.84 WLVRR248 pKa = 11.84 KK249 pKa = 9.21 LAGRR253 pKa = 11.84 RR254 pKa = 11.84 NRR256 pKa = 11.84 LAVQRR261 pKa = 4.27
Molecular weight: 28.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.326
IPC_protein 10.262
Toseland 10.467
ProMoST 10.496
Dawson 10.57
Bjellqvist 10.35
Wikipedia 10.818
Rodwell 10.584
Grimsley 10.628
Solomon 10.73
Lehninger 10.687
Nozaki 10.511
DTASelect 10.335
Thurlkill 10.482
EMBOSS 10.877
Sillero 10.526
Patrickios 10.292
IPC_peptide 10.73
IPC2_peptide 9.736
IPC2.peptide.svr19 8.694
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5169
0
5169
1739259
26
4699
336.5
36.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.35 ± 0.039
1.042 ± 0.013
5.468 ± 0.027
6.092 ± 0.033
3.618 ± 0.021
8.444 ± 0.036
2.251 ± 0.016
4.54 ± 0.024
3.024 ± 0.029
11.756 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.272 ± 0.019
2.796 ± 0.026
5.031 ± 0.027
4.229 ± 0.023
7.206 ± 0.037
5.471 ± 0.021
4.464 ± 0.03
6.985 ± 0.032
1.447 ± 0.016
2.516 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here