Kocuria coralli
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2933 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J5L0G3|A0A5J5L0G3_9MICC Protein translocase subunit SecF OS=Kocuria coralli OX=1461025 GN=secF PE=4 SV=1
MM1 pKa = 6.1 TTTRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 CAAAALAAAFVLAGCSGGDD26 pKa = 3.45 DD27 pKa = 4.12 GEE29 pKa = 4.42 GAEE32 pKa = 4.64 ADD34 pKa = 3.78 GGSGSFPSQPINLIVPYY51 pKa = 10.1 AAGGSADD58 pKa = 3.29 STARR62 pKa = 11.84 QLAAVAQDD70 pKa = 3.34 TCGAPIIIRR79 pKa = 11.84 NATGGSGAVGFQATLSSPADD99 pKa = 3.99 GYY101 pKa = 9.26 TVGSASIEE109 pKa = 4.1 LTMLDD114 pKa = 3.34 HH115 pKa = 6.45 MGISEE120 pKa = 4.22 IHH122 pKa = 6.51 PDD124 pKa = 3.76 DD125 pKa = 4.03 YY126 pKa = 11.71 EE127 pKa = 6.64 GILQYY132 pKa = 10.59 SEE134 pKa = 3.85 QPIVFGVPADD144 pKa = 3.65 SPYY147 pKa = 11.04 QSLQDD152 pKa = 4.51 VIDD155 pKa = 3.8 SDD157 pKa = 4.88 DD158 pKa = 3.77 RR159 pKa = 11.84 VTVATSGTGGIYY171 pKa = 10.18 HH172 pKa = 7.46 LGYY175 pKa = 11.01 EE176 pKa = 4.51 SMALAAGIDD185 pKa = 3.73 DD186 pKa = 3.7 RR187 pKa = 11.84 TVNVPFDD194 pKa = 3.72 GAASALQAALGDD206 pKa = 3.73 QTAMVSVGAAEE217 pKa = 4.05 MRR219 pKa = 11.84 PHH221 pKa = 6.95 IEE223 pKa = 3.81 SGAIRR228 pKa = 11.84 PLAIAGDD235 pKa = 3.9 PVDD238 pKa = 4.77 YY239 pKa = 10.82 LPEE242 pKa = 4.43 DD243 pKa = 3.76 VPTLHH248 pKa = 6.97 EE249 pKa = 4.74 LGIDD253 pKa = 3.79 SEE255 pKa = 4.84 TTAILGLYY263 pKa = 9.55 APAGVPQDD271 pKa = 3.78 RR272 pKa = 11.84 IEE274 pKa = 4.54 LLNEE278 pKa = 3.99 CFNEE282 pKa = 4.1 ARR284 pKa = 11.84 EE285 pKa = 4.08 SDD287 pKa = 3.69 QFSDD291 pKa = 3.72 YY292 pKa = 11.06 MEE294 pKa = 4.37 TTGLNQRR301 pKa = 11.84 YY302 pKa = 9.18 RR303 pKa = 11.84 DD304 pKa = 3.82 AQEE307 pKa = 4.29 FDD309 pKa = 2.92 QFMGEE314 pKa = 3.96 EE315 pKa = 4.02 YY316 pKa = 10.87 DD317 pKa = 4.53 RR318 pKa = 11.84 FGEE321 pKa = 4.45 LIDD324 pKa = 4.44 EE325 pKa = 4.62 IGMGVHH331 pKa = 7.08
Molecular weight: 34.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.884
IPC_protein 3.897
Toseland 3.681
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.215
Thurlkill 3.719
EMBOSS 3.808
Sillero 4.012
Patrickios 1.291
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.898
Protein with the highest isoelectric point:
>tr|A0A5J5KZH5|A0A5J5KZH5_9MICC Acetamidase OS=Kocuria coralli OX=1461025 GN=FCK90_06135 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 10.04 KK16 pKa = 8.86 HH17 pKa = 4.36 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSARR35 pKa = 11.84 RR36 pKa = 11.84 SKK38 pKa = 10.82 GRR40 pKa = 11.84 ASLSAA45 pKa = 3.83
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2933
0
2933
998786
24
1646
340.5
36.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.452 ± 0.052
0.627 ± 0.01
6.056 ± 0.036
6.268 ± 0.041
2.965 ± 0.026
9.184 ± 0.04
2.191 ± 0.021
4.217 ± 0.034
2.078 ± 0.032
9.978 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.061 ± 0.02
2.023 ± 0.022
5.576 ± 0.037
3.208 ± 0.023
7.353 ± 0.047
5.764 ± 0.027
5.882 ± 0.03
8.645 ± 0.039
1.519 ± 0.02
1.954 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here