Pneumocystis murina (strain B123) (Mouse pneumocystis pneumonia agent) (Pneumocystis carinii f. sp. muris)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Pneumocystidomycetes; Pneumocystidales; Pneumocystidaceae; Pneumocystis; Pneumocystis murina

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3635 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M7NRW1|M7NRW1_PNEMU Uncharacterized protein OS=Pneumocystis murina (strain B123) OX=1069680 GN=PNEG_01772 PE=4 SV=1
MM1 pKa = 7.52GFLFVTFLFAILTLINAYY19 pKa = 9.34KK20 pKa = 10.65VLLPKK25 pKa = 10.54PGDD28 pKa = 3.01KK29 pKa = 10.07WEE31 pKa = 4.98PGLHH35 pKa = 4.55QVTWEE40 pKa = 4.09AVDD43 pKa = 3.62DD44 pKa = 4.14TSTVNVYY51 pKa = 8.07LHH53 pKa = 7.09RR54 pKa = 11.84NNDD57 pKa = 3.29NPPFYY62 pKa = 10.87LLLAAKK68 pKa = 10.31VPLKK72 pKa = 10.57DD73 pKa = 3.53GKK75 pKa = 10.9ADD77 pKa = 3.45VPIPYY82 pKa = 9.95NVIPGSEE89 pKa = 4.12YY90 pKa = 10.69TILLTSLNPYY100 pKa = 10.12DD101 pKa = 4.43VYY103 pKa = 10.78TSSGGFEE110 pKa = 3.54ITEE113 pKa = 4.4AKK115 pKa = 10.5DD116 pKa = 3.39GLKK119 pKa = 10.36GVCVDD124 pKa = 3.64YY125 pKa = 11.47LKK127 pKa = 11.01DD128 pKa = 4.43DD129 pKa = 4.12GSSTIGPIPGDD140 pKa = 3.63TRR142 pKa = 11.84PVTTSLLPPSPTPPSPAPPAPTPAPPAPTPPSPEE176 pKa = 4.04PPAPAPPEE184 pKa = 4.34GEE186 pKa = 4.01VEE188 pKa = 4.15NCDD191 pKa = 3.34EE192 pKa = 4.5CDD194 pKa = 3.18EE195 pKa = 4.69CEE197 pKa = 4.11EE198 pKa = 4.75CGEE201 pKa = 4.32CEE203 pKa = 4.04PGEE206 pKa = 4.65GCDD209 pKa = 5.54CDD211 pKa = 5.89ADD213 pKa = 4.04DD214 pKa = 6.09HH215 pKa = 8.3GDD217 pKa = 3.56DD218 pKa = 6.06DD219 pKa = 4.57YY220 pKa = 12.16DD221 pKa = 4.13KK222 pKa = 11.67DD223 pKa = 3.7EE224 pKa = 4.7DD225 pKa = 4.34HH226 pKa = 6.7GHH228 pKa = 5.79SHH230 pKa = 8.01DD231 pKa = 6.22DD232 pKa = 5.39DD233 pKa = 7.24DD234 pKa = 7.67DD235 pKa = 7.27DD236 pKa = 7.59DD237 pKa = 6.37DD238 pKa = 5.42DD239 pKa = 6.07HH240 pKa = 7.82EE241 pKa = 5.24NDD243 pKa = 5.25HH244 pKa = 7.68DD245 pKa = 6.71DD246 pKa = 5.89DD247 pKa = 7.59DD248 pKa = 7.37DD249 pKa = 7.3DD250 pKa = 6.42DD251 pKa = 4.86DD252 pKa = 5.67HH253 pKa = 7.29GHH255 pKa = 6.04SHH257 pKa = 8.03DD258 pKa = 5.63DD259 pKa = 3.93DD260 pKa = 7.02DD261 pKa = 6.22DD262 pKa = 4.41GHH264 pKa = 7.62DD265 pKa = 4.22HH266 pKa = 7.45GEE268 pKa = 4.48DD269 pKa = 3.35KK270 pKa = 11.38NKK272 pKa = 10.03LANIGSTNIVTRR284 pKa = 11.84FWLTMMATISTILFLL299 pKa = 5.42

Molecular weight: 32.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M7P8Q4|M7P8Q4_PNEMU Protein kinase domain-containing protein OS=Pneumocystis murina (strain B123) OX=1069680 GN=PNEG_01497 PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.19KK5 pKa = 8.98TFRR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.78IILARR15 pKa = 11.84AQRR18 pKa = 11.84QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TNNRR34 pKa = 11.84IRR36 pKa = 11.84YY37 pKa = 5.66NAKK40 pKa = 8.4RR41 pKa = 11.84KK42 pKa = 8.21HH43 pKa = 4.15WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 11.1LNII51 pKa = 3.83

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3635

0

3635

1664453

45

5058

457.9

52.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.177 ± 0.031

1.62 ± 0.024

5.419 ± 0.025

7.017 ± 0.04

4.914 ± 0.029

4.272 ± 0.038

2.157 ± 0.017

8.665 ± 0.045

8.532 ± 0.047

9.921 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.076 ± 0.014

6.239 ± 0.038

3.632 ± 0.027

3.593 ± 0.022

4.413 ± 0.026

8.713 ± 0.047

5.046 ± 0.028

4.741 ± 0.029

1.067 ± 0.012

3.787 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski