Sunxiuqinia dokdonensis
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4693 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0L8VCW9|A0A0L8VCW9_9BACT Dihydroorotate dehydrogenase OS=Sunxiuqinia dokdonensis OX=1409788 GN=pyrD PE=3 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.16 KK3 pKa = 9.98 IIPIAVSVLMIGLVACEE20 pKa = 4.1 PEE22 pKa = 4.44 EE23 pKa = 4.45 VAFDD27 pKa = 3.65 PAADD31 pKa = 3.46 VFVITKK37 pKa = 8.49 TVATEE42 pKa = 4.06 NEE44 pKa = 3.66 VDD46 pKa = 3.7 TVYY49 pKa = 11.42 GLALHH54 pKa = 7.0 AFANKK59 pKa = 9.21 PMQSVKK65 pKa = 8.98 VTSVDD70 pKa = 2.85 NTTYY74 pKa = 11.05 DD75 pKa = 3.36 LEE77 pKa = 4.67 SYY79 pKa = 10.19 EE80 pKa = 4.29 GYY82 pKa = 9.9 PYY84 pKa = 11.09 DD85 pKa = 5.78 FYY87 pKa = 11.75 AQTEE91 pKa = 4.14 DD92 pKa = 4.16 DD93 pKa = 4.67 DD94 pKa = 5.72 FSAEE98 pKa = 4.09 MPEE101 pKa = 3.9 SGAYY105 pKa = 9.65 SFNIVAQSGEE115 pKa = 4.2 TSTLSDD121 pKa = 3.78 NLSDD125 pKa = 4.27 DD126 pKa = 4.03 VIYY129 pKa = 8.77 PTDD132 pKa = 3.71 TIKK135 pKa = 11.16 YY136 pKa = 10.15 AFDD139 pKa = 4.1 DD140 pKa = 4.32 AQNKK144 pKa = 7.78 MKK146 pKa = 9.68 LTWTEE151 pKa = 3.95 IEE153 pKa = 4.2 DD154 pKa = 3.59 ADD156 pKa = 3.98 YY157 pKa = 11.3 LIVKK161 pKa = 8.7 MFEE164 pKa = 3.8 QDD166 pKa = 3.36 DD167 pKa = 4.12 DD168 pKa = 4.13 QVFQSSSLLGDD179 pKa = 3.42 KK180 pKa = 10.81 EE181 pKa = 4.58 EE182 pKa = 4.28 YY183 pKa = 9.72 TISASGSGWASDD195 pKa = 3.58 FQPADD200 pKa = 3.02 GATYY204 pKa = 10.07 IIQLDD209 pKa = 3.64 AFKK212 pKa = 11.24 YY213 pKa = 9.95 EE214 pKa = 4.2 SGQNGVNLQAKK225 pKa = 9.64 SISLQEE231 pKa = 4.12 IVWGEE236 pKa = 3.86 EE237 pKa = 3.67
Molecular weight: 26.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.706
IPC_protein 3.706
Toseland 3.49
ProMoST 3.846
Dawson 3.694
Bjellqvist 3.872
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.643
Nozaki 3.808
DTASelect 4.024
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.834
Patrickios 1.125
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A0L8V9D6|A0A0L8V9D6_9BACT Dehydrogenase OS=Sunxiuqinia dokdonensis OX=1409788 GN=NC99_21860 PE=4 SV=1
MM1 pKa = 7.53 SLLLLTSNTQPRR13 pKa = 11.84 FRR15 pKa = 11.84 GPINVGNRR23 pKa = 11.84 SHH25 pKa = 7.1 PRR27 pKa = 11.84 RR28 pKa = 11.84 PKK30 pKa = 9.95 FSFRR34 pKa = 11.84 TLLRR38 pKa = 11.84 KK39 pKa = 9.25 AVHH42 pKa = 6.56 RR43 pKa = 11.84 KK44 pKa = 8.9
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.096
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.501
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.237
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.129
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4693
0
4693
1442061
37
2867
307.3
34.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.985 ± 0.037
0.816 ± 0.015
5.604 ± 0.028
6.827 ± 0.033
5.158 ± 0.027
6.793 ± 0.036
1.973 ± 0.014
6.896 ± 0.036
6.534 ± 0.037
9.642 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.475 ± 0.017
5.089 ± 0.028
3.809 ± 0.021
4.004 ± 0.026
4.363 ± 0.025
6.255 ± 0.028
5.163 ± 0.037
6.456 ± 0.023
1.285 ± 0.015
3.874 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here