Penicillium brasilianum
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11935 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F7VJ14|A0A0F7VJ14_PENBI Aspartate aminotransferase OS=Penicillium brasilianum OX=104259 GN=PMG11_04320 PE=3 SV=1
MM1 pKa = 7.59 GFLKK5 pKa = 10.74 KK6 pKa = 9.62 LTLFAAFASLAAALPYY22 pKa = 10.57 GEE24 pKa = 5.19 RR25 pKa = 11.84 PPTLVNLAAVAPTTTTAAAASTSSTSVTTSATSISTSISTSTSTSTSTSTSTSTSTSNGIKK86 pKa = 9.83 IVNNLDD92 pKa = 3.1 QTVYY96 pKa = 10.51 LWSTSDD102 pKa = 3.32 TSSDD106 pKa = 3.48 MQTLTSGSTYY116 pKa = 10.16 TEE118 pKa = 3.78 TWRR121 pKa = 11.84 TNSDD125 pKa = 2.95 GGGISIKK132 pKa = 9.88 MATSTSEE139 pKa = 4.03 SSVLQFEE146 pKa = 4.63 YY147 pKa = 10.45 TKK149 pKa = 10.85 SDD151 pKa = 3.29 EE152 pKa = 4.34 TLWWDD157 pKa = 4.12 LSSINLDD164 pKa = 3.34 SDD166 pKa = 4.13 SEE168 pKa = 4.72 FITSGFAVTTDD179 pKa = 3.93 DD180 pKa = 4.78 SSCTTASCAAGDD192 pKa = 3.94 TDD194 pKa = 5.88 CADD197 pKa = 3.88 SYY199 pKa = 11.7 QNPDD203 pKa = 3.45 DD204 pKa = 4.23 VDD206 pKa = 3.66 TRR208 pKa = 11.84 SCSASAAFTMTIGG221 pKa = 3.51
Molecular weight: 22.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.77
IPC_protein 3.77
Toseland 3.541
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.164
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.884
Patrickios 1.901
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A0F7TRT7|A0A0F7TRT7_PENBI Acyl_transf_3 domain-containing protein OS=Penicillium brasilianum OX=104259 GN=PMG11_06420 PE=4 SV=1
MM1 pKa = 7.56 LCLRR5 pKa = 11.84 CRR7 pKa = 11.84 ALPSFQATVSAMRR20 pKa = 11.84 TPMTRR25 pKa = 11.84 ITQFTPQTSLTSTARR40 pKa = 11.84 LSSRR44 pKa = 11.84 PFSTTLLSTPTRR56 pKa = 11.84 LQPSQTLSVSPRR68 pKa = 11.84 TPSTSALSTPSISSLLPPQSRR89 pKa = 11.84 SFSATASLGVKK100 pKa = 9.98 RR101 pKa = 11.84 NTFRR105 pKa = 11.84 PSRR108 pKa = 11.84 RR109 pKa = 11.84 VQKK112 pKa = 10.38 RR113 pKa = 11.84 RR114 pKa = 11.84 SGFLARR120 pKa = 11.84 NTDD123 pKa = 2.92 RR124 pKa = 11.84 KK125 pKa = 10.79 GRR127 pKa = 11.84 LTLIRR132 pKa = 11.84 RR133 pKa = 11.84 RR134 pKa = 11.84 LKK136 pKa = 10.46 GRR138 pKa = 11.84 KK139 pKa = 8.86 AMSWW143 pKa = 3.05
Molecular weight: 15.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.417
IPC2_protein 10.891
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.266
Grimsley 12.691
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 11.974
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11426
509
11935
5855176
47
6077
490.6
54.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.575 ± 0.019
1.245 ± 0.009
5.618 ± 0.015
6.11 ± 0.022
3.8 ± 0.013
6.776 ± 0.022
2.425 ± 0.01
5.048 ± 0.016
4.543 ± 0.018
9.052 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.196 ± 0.008
3.645 ± 0.011
6.02 ± 0.024
4.087 ± 0.016
6.068 ± 0.02
8.44 ± 0.024
5.965 ± 0.016
6.115 ± 0.014
1.49 ± 0.009
2.784 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here