Selenomonas ruminantium
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2579 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1K1PXL5|A0A1K1PXL5_SELRU 30S ribosomal protein S6 OS=Selenomonas ruminantium OX=971 GN=rpsF PE=3 SV=1
MM1 pKa = 7.01 GTIALIGIGVFLGWLMFRR19 pKa = 11.84 PKK21 pKa = 10.53 NDD23 pKa = 3.19 NRR25 pKa = 11.84 RR26 pKa = 11.84 MSSADD31 pKa = 3.63 LPQKK35 pKa = 10.21 QQLTKK40 pKa = 10.35 QQQTKK45 pKa = 7.86 QQVTTGQRR53 pKa = 11.84 PQVSSSLQHH62 pKa = 6.71 RR63 pKa = 11.84 EE64 pKa = 4.05 NNSGNHH70 pKa = 4.76 TLRR73 pKa = 11.84 NVAGGMVAGAVLGHH87 pKa = 6.25 MLSGDD92 pKa = 3.82 HH93 pKa = 6.67 KK94 pKa = 10.94 AEE96 pKa = 3.78 AHH98 pKa = 5.0 EE99 pKa = 4.36 TVNNYY104 pKa = 8.73 NVYY107 pKa = 10.63 NDD109 pKa = 4.28 YY110 pKa = 11.17 YY111 pKa = 10.91 DD112 pKa = 4.57 HH113 pKa = 6.96 EE114 pKa = 5.35 HH115 pKa = 6.84 LAQDD119 pKa = 4.72 EE120 pKa = 4.24 DD121 pKa = 4.32 VDD123 pKa = 3.83 WDD125 pKa = 4.16 DD126 pKa = 5.54 DD127 pKa = 4.7 YY128 pKa = 12.33 DD129 pKa = 3.73 MLAYY133 pKa = 10.46 DD134 pKa = 4.82 EE135 pKa = 6.22 DD136 pKa = 6.48 DD137 pKa = 4.3 DD138 pKa = 6.28 DD139 pKa = 5.97 SYY141 pKa = 12.31 NSYY144 pKa = 11.58 DD145 pKa = 3.79 DD146 pKa = 5.53 DD147 pKa = 6.75 DD148 pKa = 4.14 YY149 pKa = 11.91 TGYY152 pKa = 11.1 EE153 pKa = 4.12 DD154 pKa = 5.69 DD155 pKa = 5.5 SYY157 pKa = 11.93 DD158 pKa = 4.3 SSSDD162 pKa = 3.35 WGSDD166 pKa = 3.28 SYY168 pKa = 12.11 DD169 pKa = 5.09 DD170 pKa = 4.83 GGDD173 pKa = 3.09 WW174 pKa = 4.09
Molecular weight: 19.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.935
IPC_protein 3.961
Toseland 3.732
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.948
Rodwell 3.795
Grimsley 3.643
Solomon 3.973
Lehninger 3.935
Nozaki 4.088
DTASelect 4.406
Thurlkill 3.795
EMBOSS 3.948
Sillero 4.101
Patrickios 1.952
IPC_peptide 3.961
IPC2_peptide 4.075
IPC2.peptide.svr19 3.968
Protein with the highest isoelectric point:
>tr|A0A1K1NKK7|A0A1K1NKK7_SELRU Uncharacterized protein OS=Selenomonas ruminantium OX=971 GN=SAMN02910323_1388 PE=4 SV=1
MM1 pKa = 7.73 KK2 pKa = 8.67 MTFQPNNHH10 pKa = 5.38 WRR12 pKa = 11.84 KK13 pKa = 7.47 KK14 pKa = 6.28 THH16 pKa = 5.76 GFRR19 pKa = 11.84 EE20 pKa = 3.92 RR21 pKa = 11.84 MKK23 pKa = 10.1 TKK25 pKa = 10.35 GGRR28 pKa = 11.84 LVLKK32 pKa = 10.33 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.87 KK41 pKa = 10.62 LSAA44 pKa = 4.03
Molecular weight: 5.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.382
IPC2_protein 10.833
IPC_protein 12.384
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.369
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.036
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2579
0
2579
824202
37
2894
319.6
35.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.31 ± 0.053
1.259 ± 0.018
5.552 ± 0.044
7.007 ± 0.053
3.929 ± 0.031
7.452 ± 0.052
2.007 ± 0.024
6.425 ± 0.045
6.252 ± 0.042
9.487 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.224 ± 0.026
3.891 ± 0.04
3.682 ± 0.026
3.748 ± 0.037
4.744 ± 0.046
5.203 ± 0.035
5.136 ± 0.043
7.173 ± 0.045
1.044 ± 0.021
3.475 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here