Gluconacetobacter liquefaciens (Acetobacter liquefaciens)
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3696 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A370G705|A0A370G705_GLULI L-serine dehydratase OS=Gluconacetobacter liquefaciens OX=89584 GN=C7453_102369 PE=3 SV=1
MM1 pKa = 7.41 SVFNALTTAVSGINAQSTAFTNLSNNIANSQTVGYY36 pKa = 9.73 KK37 pKa = 9.3 ATTTAFQDD45 pKa = 4.32 FVAGSLGKK53 pKa = 9.75 NASSADD59 pKa = 3.27 ISDD62 pKa = 3.47 SVAAVTVQHH71 pKa = 6.58 VDD73 pKa = 3.32 NQGTAATSTDD83 pKa = 3.4 SLATAISGSGLFVVSKK99 pKa = 10.61 EE100 pKa = 4.29 SGASTSTNTQFQSQNYY116 pKa = 4.93 YY117 pKa = 8.09 TRR119 pKa = 11.84 NGEE122 pKa = 4.18 VYY124 pKa = 10.28 KK125 pKa = 11.11 NNSGYY130 pKa = 10.79 LVNTSGYY137 pKa = 9.17 YY138 pKa = 10.07 LDD140 pKa = 5.48 GYY142 pKa = 9.95 MVDD145 pKa = 3.85 PTTGSLNASLTQLNVANVAFRR166 pKa = 11.84 PTQTTTLTLSATVGTMPSSNASYY189 pKa = 8.96 TAADD193 pKa = 3.57 AQSYY197 pKa = 6.01 TTAPVTTYY205 pKa = 10.67 DD206 pKa = 4.74 ASATPHH212 pKa = 6.76 KK213 pKa = 10.27 IALSWTQSGTNPLVWNVSAYY233 pKa = 10.49 DD234 pKa = 3.66 ADD236 pKa = 4.06 GTGTIASNSYY246 pKa = 10.25 QVTFDD251 pKa = 3.58 SSGSLASVADD261 pKa = 3.63 STTGKK266 pKa = 9.05 TIGSTLTGASASIPITADD284 pKa = 3.28 YY285 pKa = 11.22 NGVAQNITLDD295 pKa = 3.52 LGTIGGTSGTVMAASTGTASTNQANALTASGTTLTMSKK333 pKa = 10.43 SDD335 pKa = 3.97 VIGTTTGSNQTSMTSPTVANGTSVAAKK362 pKa = 6.92 WTQTAASPPTWSLSLVDD379 pKa = 5.33 PYY381 pKa = 10.57 DD382 pKa = 3.78 TSSSSPVSSDD392 pKa = 2.84 TYY394 pKa = 10.81 NVVFNTDD401 pKa = 2.96 GSVQSVTDD409 pKa = 3.34 STTGVTTSLSALSATIGGTSYY430 pKa = 10.41 TVDD433 pKa = 3.72 LSQTTLSTASSLATDD448 pKa = 4.18 TTALQSDD455 pKa = 4.88 SIASGTYY462 pKa = 8.82 QGVEE466 pKa = 3.91 IEE468 pKa = 4.4 SDD470 pKa = 3.75 GSVMAQFDD478 pKa = 3.92 TGTQLIGKK486 pKa = 8.8 IALANFANVDD496 pKa = 3.46 GLNAVDD502 pKa = 3.98 GQAYY506 pKa = 6.85 TATASSGDD514 pKa = 3.69 AKK516 pKa = 10.67 IGLAGANGTGTLSVGSVEE534 pKa = 5.47 SSTTDD539 pKa = 3.27 LTSDD543 pKa = 3.78 LSALIVAQEE552 pKa = 4.23 AYY554 pKa = 10.14 SANTKK559 pKa = 9.96 IVTTADD565 pKa = 3.08 QLLQTTIAMKK575 pKa = 10.31 QQ576 pKa = 3.01
Molecular weight: 58.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.77
IPC_protein 3.808
Toseland 3.567
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.808
Rodwell 3.63
Grimsley 3.465
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.266
Thurlkill 3.63
EMBOSS 3.808
Sillero 3.935
Patrickios 0.528
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A370G1M2|A0A370G1M2_GLULI Sodium/proton antiporter (CPA1 family) OS=Gluconacetobacter liquefaciens OX=89584 GN=C7453_10940 PE=4 SV=1
MM1 pKa = 7.84 DD2 pKa = 5.7 RR3 pKa = 11.84 DD4 pKa = 4.04 GPVARR9 pKa = 11.84 QGDD12 pKa = 4.01 RR13 pKa = 11.84 RR14 pKa = 11.84 VHH16 pKa = 6.73 LLLQRR21 pKa = 11.84 DD22 pKa = 3.81 GWAVNPKK29 pKa = 10.03 RR30 pKa = 11.84 IYY32 pKa = 10.41 RR33 pKa = 11.84 LYY35 pKa = 10.4 KK36 pKa = 9.91 ALGMQLRR43 pKa = 11.84 NKK45 pKa = 9.35 VPRR48 pKa = 11.84 HH49 pKa = 4.93 RR50 pKa = 11.84 VTAKK54 pKa = 9.96 VRR56 pKa = 11.84 EE57 pKa = 4.2 DD58 pKa = 3.35 RR59 pKa = 11.84 CPATHH64 pKa = 6.1 THH66 pKa = 7.11 ANDD69 pKa = 2.91 SWAKK73 pKa = 10.69 DD74 pKa = 3.65 CVHH77 pKa = 7.18 DD78 pKa = 5.46 RR79 pKa = 11.84 RR80 pKa = 11.84 ATDD83 pKa = 3.43 RR84 pKa = 11.84 RR85 pKa = 11.84 ILTVIDD91 pKa = 3.11 IFSRR95 pKa = 11.84 FSLATDD101 pKa = 3.15 PRR103 pKa = 11.84 FSYY106 pKa = 10.35 RR107 pKa = 11.84 GEE109 pKa = 3.97 NVAQTLEE116 pKa = 4.52 RR117 pKa = 11.84 ICEE120 pKa = 3.89
Molecular weight: 14.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.458
IPC_protein 10.335
Toseland 10.526
ProMoST 10.262
Dawson 10.628
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.716
Grimsley 10.687
Solomon 10.76
Lehninger 10.73
Nozaki 10.54
DTASelect 10.365
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.57
Patrickios 10.452
IPC_peptide 10.774
IPC2_peptide 9.604
IPC2.peptide.svr19 8.588
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3696
0
3696
1239589
29
2368
335.4
36.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.724 ± 0.059
0.932 ± 0.015
5.693 ± 0.031
4.693 ± 0.039
3.443 ± 0.026
8.914 ± 0.04
2.406 ± 0.018
4.791 ± 0.026
2.126 ± 0.031
10.32 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.575 ± 0.018
2.453 ± 0.03
5.745 ± 0.033
3.253 ± 0.026
7.752 ± 0.044
5.386 ± 0.036
5.702 ± 0.035
7.378 ± 0.034
1.432 ± 0.016
2.282 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here