Arthrobacter phage Daob

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Coralvirus; unclassified Coralvirus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KFC8|A0A3G2KFC8_9CAUD Head-to-tail stopper OS=Arthrobacter phage Daob OX=2419954 GN=11 PE=4 SV=1
MM1 pKa = 7.48GNVSDD6 pKa = 4.32WEE8 pKa = 4.43VNGDD12 pKa = 3.8GFATGPVIEE21 pKa = 5.05VEE23 pKa = 4.19NGDD26 pKa = 3.71NPVTVSAVWTDD37 pKa = 3.14EE38 pKa = 5.03DD39 pKa = 4.99GVCVSVDD46 pKa = 3.62AGDD49 pKa = 4.21EE50 pKa = 3.99PLKK53 pKa = 11.13ARR55 pKa = 11.84VAYY58 pKa = 8.99RR59 pKa = 11.84VCAAIMEE66 pKa = 4.72LAAVSAPEE74 pKa = 4.29LSSSS78 pKa = 3.39

Molecular weight: 8.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KFI0|A0A3G2KFI0_9CAUD MerR-like helix-turn-helix DNA binding domain protein OS=Arthrobacter phage Daob OX=2419954 GN=70 PE=4 SV=1
MM1 pKa = 7.52GRR3 pKa = 11.84PPLPVGTWGAVRR15 pKa = 11.84VEE17 pKa = 4.99RR18 pKa = 11.84IAGGYY23 pKa = 7.67RR24 pKa = 11.84ARR26 pKa = 11.84ARR28 pKa = 11.84FRR30 pKa = 11.84DD31 pKa = 3.49FDD33 pKa = 3.39GRR35 pKa = 11.84TRR37 pKa = 11.84DD38 pKa = 3.38VEE40 pKa = 4.21RR41 pKa = 11.84SGKK44 pKa = 8.31TKK46 pKa = 10.24GAARR50 pKa = 11.84AALTADD56 pKa = 3.91LNDD59 pKa = 3.45RR60 pKa = 11.84TAPAGEE66 pKa = 4.98EE67 pKa = 3.86ITASTRR73 pKa = 11.84LQLVAEE79 pKa = 4.79IWRR82 pKa = 11.84AEE84 pKa = 3.78KK85 pKa = 10.03WPNLAEE91 pKa = 4.22NSRR94 pKa = 11.84KK95 pKa = 9.81RR96 pKa = 11.84YY97 pKa = 9.47RR98 pKa = 11.84DD99 pKa = 3.27ALEE102 pKa = 4.26DD103 pKa = 4.42HH104 pKa = 6.97ILPGLGALTVSEE116 pKa = 4.59CSVTRR121 pKa = 11.84IDD123 pKa = 4.51RR124 pKa = 11.84FLKK127 pKa = 9.45ATAAGTGAPSAKK139 pKa = 9.43VCRR142 pKa = 11.84SVLSGILGLAVRR154 pKa = 11.84HH155 pKa = 5.79GAAATNPVRR164 pKa = 11.84DD165 pKa = 3.64VAGITVTPKK174 pKa = 10.38EE175 pKa = 4.0IRR177 pKa = 11.84ALTLEE182 pKa = 4.69EE183 pKa = 3.5IRR185 pKa = 11.84AARR188 pKa = 11.84SAVRR192 pKa = 11.84SWQLGEE198 pKa = 4.0PLAEE202 pKa = 4.04GRR204 pKa = 11.84PRR206 pKa = 11.84RR207 pKa = 11.84GRR209 pKa = 11.84PPTQDD214 pKa = 3.83LLDD217 pKa = 4.06ILDD220 pKa = 4.63LLLATGARR228 pKa = 11.84IGEE231 pKa = 4.23LLAIRR236 pKa = 11.84WSDD239 pKa = 3.03VDD241 pKa = 5.53LEE243 pKa = 5.04DD244 pKa = 3.69GTLTISGTIVSTEE257 pKa = 4.01DD258 pKa = 2.83KK259 pKa = 9.71PARR262 pKa = 11.84LIRR265 pKa = 11.84QAHH268 pKa = 6.06PKK270 pKa = 10.08SSTSRR275 pKa = 11.84RR276 pKa = 11.84RR277 pKa = 11.84LALPPFAIDD286 pKa = 2.93ALMRR290 pKa = 11.84RR291 pKa = 11.84RR292 pKa = 11.84LAVTVANVHH301 pKa = 6.45DD302 pKa = 4.25VVFPSTEE309 pKa = 3.7GTLRR313 pKa = 11.84DD314 pKa = 3.73PGSVRR319 pKa = 11.84KK320 pKa = 9.26QLAKK324 pKa = 10.57VLAPAGLGWVTPHH337 pKa = 6.16VFRR340 pKa = 11.84KK341 pKa = 7.9TVATALDD348 pKa = 3.57AAEE351 pKa = 4.78DD352 pKa = 3.77LRR354 pKa = 11.84TAADD358 pKa = 3.66QLGHH362 pKa = 7.2AGTDD366 pKa = 3.2VTRR369 pKa = 11.84RR370 pKa = 11.84HH371 pKa = 5.53YY372 pKa = 9.64VQKK375 pKa = 9.08THH377 pKa = 6.32QGPDD381 pKa = 2.84ARR383 pKa = 11.84ATLEE387 pKa = 3.9QLVRR391 pKa = 11.84PAGPP395 pKa = 3.2

Molecular weight: 42.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

12102

38

711

170.5

18.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.659 ± 0.47

0.835 ± 0.108

5.47 ± 0.172

6.619 ± 0.278

2.628 ± 0.206

9.808 ± 0.459

1.396 ± 0.138

3.586 ± 0.191

3.603 ± 0.23

8.982 ± 0.318

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.083

2.363 ± 0.152

5.594 ± 0.334

3.057 ± 0.17

7.784 ± 0.419

5.404 ± 0.242

5.371 ± 0.294

8.651 ± 0.394

2.115 ± 0.155

1.95 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski