Amino acid dipepetide frequency for Arthrobacter phage Daob

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.651AlaAla: 21.651 ± 2.123
0.744AlaCys: 0.744 ± 0.275
7.851AlaAsp: 7.851 ± 0.875
7.355AlaGlu: 7.355 ± 0.867
2.975AlaPhe: 2.975 ± 0.584
15.04AlaGly: 15.04 ± 1.071
1.405AlaHis: 1.405 ± 0.275
4.297AlaIle: 4.297 ± 0.669
2.231AlaLys: 2.231 ± 0.523
10.826AlaLeu: 10.826 ± 0.844
2.81AlaMet: 2.81 ± 0.444
2.81AlaAsn: 2.81 ± 0.496
5.289AlaPro: 5.289 ± 0.829
3.223AlaGln: 3.223 ± 0.56
9.586AlaArg: 9.586 ± 1.117
6.859AlaSer: 6.859 ± 0.95
5.702AlaThr: 5.702 ± 0.721
10.991AlaVal: 10.991 ± 0.781
2.975AlaTrp: 2.975 ± 0.429
1.983AlaTyr: 1.983 ± 0.394
0.0AlaXaa: 0.0 ± 0.0
Cys
0.248CysAla: 0.248 ± 0.119
0.0CysCys: 0.0 ± 0.0
0.413CysAsp: 0.413 ± 0.17
0.578CysGlu: 0.578 ± 0.214
0.0CysPhe: 0.0 ± 0.0
1.735CysGly: 1.735 ± 0.401
0.165CysHis: 0.165 ± 0.118
0.331CysIle: 0.331 ± 0.148
0.496CysLys: 0.496 ± 0.205
0.413CysLeu: 0.413 ± 0.21
0.083CysMet: 0.083 ± 0.081
0.083CysAsn: 0.083 ± 0.088
0.083CysPro: 0.083 ± 0.094
0.496CysGln: 0.496 ± 0.193
1.157CysArg: 1.157 ± 0.317
0.909CysSer: 0.909 ± 0.274
0.248CysThr: 0.248 ± 0.139
0.496CysVal: 0.496 ± 0.2
0.331CysTrp: 0.331 ± 0.15
0.083CysTyr: 0.083 ± 0.088
0.0CysXaa: 0.0 ± 0.0
Asp
5.371AspAla: 5.371 ± 0.594
0.496AspCys: 0.496 ± 0.205
2.562AspAsp: 2.562 ± 0.401
3.306AspGlu: 3.306 ± 0.616
1.57AspPhe: 1.57 ± 0.316
7.189AspGly: 7.189 ± 0.842
1.24AspHis: 1.24 ± 0.303
1.074AspIle: 1.074 ± 0.305
1.818AspLys: 1.818 ± 0.383
6.198AspLeu: 6.198 ± 0.632
0.661AspMet: 0.661 ± 0.231
0.992AspAsn: 0.992 ± 0.263
4.38AspPro: 4.38 ± 0.591
1.487AspGln: 1.487 ± 0.354
3.801AspArg: 3.801 ± 0.706
2.727AspSer: 2.727 ± 0.447
2.231AspThr: 2.231 ± 0.426
4.793AspVal: 4.793 ± 0.84
1.405AspTrp: 1.405 ± 0.303
1.405AspTyr: 1.405 ± 0.298
0.0AspXaa: 0.0 ± 0.0
Glu
6.528GluAla: 6.528 ± 0.816
0.744GluCys: 0.744 ± 0.218
3.553GluAsp: 3.553 ± 0.575
4.793GluGlu: 4.793 ± 0.794
1.735GluPhe: 1.735 ± 0.355
5.206GluGly: 5.206 ± 0.551
0.826GluHis: 0.826 ± 0.276
4.049GluIle: 4.049 ± 0.653
2.149GluLys: 2.149 ± 0.467
6.694GluLeu: 6.694 ± 0.71
0.909GluMet: 0.909 ± 0.318
1.735GluAsn: 1.735 ± 0.306
4.71GluPro: 4.71 ± 0.68
3.306GluGln: 3.306 ± 0.601
4.049GluArg: 4.049 ± 0.548
2.727GluSer: 2.727 ± 0.512
3.388GluThr: 3.388 ± 0.565
6.694GluVal: 6.694 ± 0.674
0.992GluTrp: 0.992 ± 0.269
1.405GluTyr: 1.405 ± 0.306
0.0GluXaa: 0.0 ± 0.0
Phe
2.479PheAla: 2.479 ± 0.408
0.083PheCys: 0.083 ± 0.081
1.901PheAsp: 1.901 ± 0.338
2.562PheGlu: 2.562 ± 0.501
0.578PhePhe: 0.578 ± 0.173
2.562PheGly: 2.562 ± 0.398
0.413PheHis: 0.413 ± 0.214
1.157PheIle: 1.157 ± 0.24
1.653PheLys: 1.653 ± 0.296
2.231PheLeu: 2.231 ± 0.364
0.826PheMet: 0.826 ± 0.215
1.24PheAsn: 1.24 ± 0.33
0.992PhePro: 0.992 ± 0.325
0.331PheGln: 0.331 ± 0.151
2.314PheArg: 2.314 ± 0.434
1.57PheSer: 1.57 ± 0.368
0.992PheThr: 0.992 ± 0.282
1.405PheVal: 1.405 ± 0.339
0.413PheTrp: 0.413 ± 0.164
0.578PheTyr: 0.578 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
10.908GlyAla: 10.908 ± 1.019
0.744GlyCys: 0.744 ± 0.264
5.206GlyAsp: 5.206 ± 0.834
5.785GlyGlu: 5.785 ± 0.667
2.81GlyPhe: 2.81 ± 0.52
9.008GlyGly: 9.008 ± 1.038
1.322GlyHis: 1.322 ± 0.367
3.719GlyIle: 3.719 ± 0.753
2.975GlyLys: 2.975 ± 0.604
7.851GlyLeu: 7.851 ± 1.098
3.058GlyMet: 3.058 ± 0.509
1.24GlyAsn: 1.24 ± 0.259
6.198GlyPro: 6.198 ± 0.732
2.644GlyGln: 2.644 ± 0.379
10.164GlyArg: 10.164 ± 0.767
5.537GlySer: 5.537 ± 0.811
5.454GlyThr: 5.454 ± 0.539
7.933GlyVal: 7.933 ± 1.208
2.975GlyTrp: 2.975 ± 0.481
2.562GlyTyr: 2.562 ± 0.447
0.0GlyXaa: 0.0 ± 0.0
His
1.653HisAla: 1.653 ± 0.304
0.248HisCys: 0.248 ± 0.157
0.826HisAsp: 0.826 ± 0.282
0.661HisGlu: 0.661 ± 0.22
0.496HisPhe: 0.496 ± 0.167
1.24HisGly: 1.24 ± 0.304
0.331HisHis: 0.331 ± 0.173
0.165HisIle: 0.165 ± 0.105
0.661HisLys: 0.661 ± 0.189
1.322HisLeu: 1.322 ± 0.361
0.248HisMet: 0.248 ± 0.124
0.248HisAsn: 0.248 ± 0.128
1.157HisPro: 1.157 ± 0.307
0.826HisGln: 0.826 ± 0.242
0.992HisArg: 0.992 ± 0.259
0.413HisSer: 0.413 ± 0.158
0.496HisThr: 0.496 ± 0.217
1.24HisVal: 1.24 ± 0.347
0.083HisTrp: 0.083 ± 0.073
0.661HisTyr: 0.661 ± 0.204
0.0HisXaa: 0.0 ± 0.0
Ile
4.38IleAla: 4.38 ± 0.642
0.248IleCys: 0.248 ± 0.172
2.231IleAsp: 2.231 ± 0.397
2.479IleGlu: 2.479 ± 0.471
0.578IlePhe: 0.578 ± 0.179
3.388IleGly: 3.388 ± 0.449
0.496IleHis: 0.496 ± 0.189
0.909IleIle: 0.909 ± 0.296
0.909IleLys: 0.909 ± 0.302
3.14IleLeu: 3.14 ± 0.581
0.992IleMet: 0.992 ± 0.279
0.992IleAsn: 0.992 ± 0.32
1.405IlePro: 1.405 ± 0.311
0.992IleGln: 0.992 ± 0.277
3.967IleArg: 3.967 ± 0.687
2.644IleSer: 2.644 ± 0.52
2.479IleThr: 2.479 ± 0.426
2.892IleVal: 2.892 ± 0.426
0.661IleTrp: 0.661 ± 0.187
0.083IleTyr: 0.083 ± 0.083
0.0IleXaa: 0.0 ± 0.0
Lys
4.876LysAla: 4.876 ± 0.719
0.248LysCys: 0.248 ± 0.14
1.322LysAsp: 1.322 ± 0.361
1.901LysGlu: 1.901 ± 0.507
1.074LysPhe: 1.074 ± 0.28
2.396LysGly: 2.396 ± 0.389
0.578LysHis: 0.578 ± 0.235
0.826LysIle: 0.826 ± 0.292
2.231LysLys: 2.231 ± 0.476
2.81LysLeu: 2.81 ± 0.45
1.24LysMet: 1.24 ± 0.427
1.24LysAsn: 1.24 ± 0.286
2.396LysPro: 2.396 ± 0.562
1.983LysGln: 1.983 ± 0.352
2.231LysArg: 2.231 ± 0.437
2.479LysSer: 2.479 ± 0.476
2.644LysThr: 2.644 ± 0.428
2.231LysVal: 2.231 ± 0.403
0.661LysTrp: 0.661 ± 0.273
0.578LysTyr: 0.578 ± 0.248
0.0LysXaa: 0.0 ± 0.0
Leu
11.239LeuAla: 11.239 ± 0.978
0.909LeuCys: 0.909 ± 0.276
5.041LeuAsp: 5.041 ± 0.65
9.751LeuGlu: 9.751 ± 0.967
1.983LeuPhe: 1.983 ± 0.341
9.09LeuGly: 9.09 ± 1.014
0.496LeuHis: 0.496 ± 0.223
3.14LeuIle: 3.14 ± 0.444
3.14LeuLys: 3.14 ± 0.512
7.189LeuLeu: 7.189 ± 0.965
1.901LeuMet: 1.901 ± 0.454
2.396LeuAsn: 2.396 ± 0.433
5.537LeuPro: 5.537 ± 0.567
2.644LeuGln: 2.644 ± 0.465
6.694LeuArg: 6.694 ± 0.748
4.215LeuSer: 4.215 ± 0.691
4.793LeuThr: 4.793 ± 0.767
6.859LeuVal: 6.859 ± 1.031
1.405LeuTrp: 1.405 ± 0.357
1.405LeuTyr: 1.405 ± 0.313
0.0LeuXaa: 0.0 ± 0.0
Met
2.562MetAla: 2.562 ± 0.446
0.248MetCys: 0.248 ± 0.138
1.487MetAsp: 1.487 ± 0.304
0.992MetGlu: 0.992 ± 0.257
0.083MetPhe: 0.083 ± 0.084
2.562MetGly: 2.562 ± 0.446
0.248MetHis: 0.248 ± 0.141
0.496MetIle: 0.496 ± 0.169
1.074MetLys: 1.074 ± 0.271
1.901MetLeu: 1.901 ± 0.348
0.413MetMet: 0.413 ± 0.165
0.413MetAsn: 0.413 ± 0.173
0.826MetPro: 0.826 ± 0.203
0.248MetGln: 0.248 ± 0.132
1.735MetArg: 1.735 ± 0.397
2.149MetSer: 2.149 ± 0.352
1.735MetThr: 1.735 ± 0.347
1.653MetVal: 1.653 ± 0.349
0.248MetTrp: 0.248 ± 0.119
0.165MetTyr: 0.165 ± 0.104
0.0MetXaa: 0.0 ± 0.0
Asn
2.479AsnAla: 2.479 ± 0.687
0.0AsnCys: 0.0 ± 0.0
1.157AsnAsp: 1.157 ± 0.273
1.487AsnGlu: 1.487 ± 0.309
0.744AsnPhe: 0.744 ± 0.305
2.81AsnGly: 2.81 ± 0.554
0.413AsnHis: 0.413 ± 0.221
0.909AsnIle: 0.909 ± 0.301
1.322AsnLys: 1.322 ± 0.427
2.066AsnLeu: 2.066 ± 0.331
0.826AsnMet: 0.826 ± 0.262
0.413AsnAsn: 0.413 ± 0.184
1.405AsnPro: 1.405 ± 0.295
0.909AsnGln: 0.909 ± 0.23
1.818AsnArg: 1.818 ± 0.418
0.744AsnSer: 0.744 ± 0.26
0.909AsnThr: 0.909 ± 0.242
2.149AsnVal: 2.149 ± 0.318
0.496AsnTrp: 0.496 ± 0.194
0.578AsnTyr: 0.578 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
9.338ProAla: 9.338 ± 0.976
0.331ProCys: 0.331 ± 0.165
3.636ProAsp: 3.636 ± 0.611
5.454ProGlu: 5.454 ± 0.784
1.074ProPhe: 1.074 ± 0.336
5.371ProGly: 5.371 ± 0.73
0.909ProHis: 0.909 ± 0.296
1.653ProIle: 1.653 ± 0.376
1.983ProLys: 1.983 ± 0.452
3.801ProLeu: 3.801 ± 0.521
1.074ProMet: 1.074 ± 0.269
0.992ProAsn: 0.992 ± 0.265
3.223ProPro: 3.223 ± 0.584
1.074ProGln: 1.074 ± 0.372
3.058ProArg: 3.058 ± 0.432
2.892ProSer: 2.892 ± 0.517
2.066ProThr: 2.066 ± 0.364
5.371ProVal: 5.371 ± 0.7
1.735ProTrp: 1.735 ± 0.301
0.909ProTyr: 0.909 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
3.636GlnAla: 3.636 ± 0.597
0.165GlnCys: 0.165 ± 0.108
1.322GlnAsp: 1.322 ± 0.32
1.818GlnGlu: 1.818 ± 0.422
0.992GlnPhe: 0.992 ± 0.289
1.983GlnGly: 1.983 ± 0.357
0.909GlnHis: 0.909 ± 0.227
1.735GlnIle: 1.735 ± 0.311
0.992GlnLys: 0.992 ± 0.248
4.215GlnLeu: 4.215 ± 0.72
0.661GlnMet: 0.661 ± 0.233
1.074GlnAsn: 1.074 ± 0.295
1.983GlnPro: 1.983 ± 0.406
0.909GlnGln: 0.909 ± 0.248
1.818GlnArg: 1.818 ± 0.417
0.992GlnSer: 0.992 ± 0.224
1.487GlnThr: 1.487 ± 0.418
2.644GlnVal: 2.644 ± 0.615
0.496GlnTrp: 0.496 ± 0.186
0.744GlnTyr: 0.744 ± 0.28
0.0GlnXaa: 0.0 ± 0.0
Arg
10.743ArgAla: 10.743 ± 1.362
0.992ArgCys: 0.992 ± 0.305
4.049ArgAsp: 4.049 ± 0.56
3.967ArgGlu: 3.967 ± 0.7
2.479ArgPhe: 2.479 ± 0.447
6.694ArgGly: 6.694 ± 0.888
1.24ArgHis: 1.24 ± 0.357
2.81ArgIle: 2.81 ± 0.494
3.306ArgLys: 3.306 ± 0.47
6.363ArgLeu: 6.363 ± 0.697
0.992ArgMet: 0.992 ± 0.242
1.818ArgAsn: 1.818 ± 0.488
4.628ArgPro: 4.628 ± 0.76
3.306ArgGln: 3.306 ± 0.541
6.28ArgArg: 6.28 ± 0.774
4.215ArgSer: 4.215 ± 0.555
5.124ArgThr: 5.124 ± 0.694
6.611ArgVal: 6.611 ± 0.54
0.826ArgTrp: 0.826 ± 0.241
1.405ArgTyr: 1.405 ± 0.316
0.0ArgXaa: 0.0 ± 0.0
Ser
6.859SerAla: 6.859 ± 0.724
0.331SerCys: 0.331 ± 0.174
2.149SerAsp: 2.149 ± 0.415
1.983SerGlu: 1.983 ± 0.297
1.653SerPhe: 1.653 ± 0.392
7.107SerGly: 7.107 ± 0.961
0.909SerHis: 0.909 ± 0.275
2.149SerIle: 2.149 ± 0.418
2.314SerLys: 2.314 ± 0.465
5.289SerLeu: 5.289 ± 0.59
1.57SerMet: 1.57 ± 0.366
1.405SerAsn: 1.405 ± 0.342
2.314SerPro: 2.314 ± 0.512
1.24SerGln: 1.24 ± 0.293
3.14SerArg: 3.14 ± 0.581
3.388SerSer: 3.388 ± 0.714
2.892SerThr: 2.892 ± 0.512
4.793SerVal: 4.793 ± 0.606
1.653SerTrp: 1.653 ± 0.287
0.909SerTyr: 0.909 ± 0.272
0.0SerXaa: 0.0 ± 0.0
Thr
7.355ThrAla: 7.355 ± 0.877
0.496ThrCys: 0.496 ± 0.253
2.81ThrAsp: 2.81 ± 0.577
2.479ThrGlu: 2.479 ± 0.455
1.735ThrPhe: 1.735 ± 0.485
5.124ThrGly: 5.124 ± 0.91
0.413ThrHis: 0.413 ± 0.208
3.058ThrIle: 3.058 ± 0.566
1.983ThrLys: 1.983 ± 0.481
4.958ThrLeu: 4.958 ± 0.547
0.826ThrMet: 0.826 ± 0.255
1.901ThrAsn: 1.901 ± 0.372
2.066ThrPro: 2.066 ± 0.393
1.157ThrGln: 1.157 ± 0.263
3.14ThrArg: 3.14 ± 0.588
2.231ThrSer: 2.231 ± 0.413
3.306ThrThr: 3.306 ± 0.558
5.702ThrVal: 5.702 ± 0.617
1.405ThrTrp: 1.405 ± 0.315
1.57ThrTyr: 1.57 ± 0.43
0.0ThrXaa: 0.0 ± 0.0
Val
9.999ValAla: 9.999 ± 0.965
0.826ValCys: 0.826 ± 0.32
4.215ValAsp: 4.215 ± 0.614
6.859ValGlu: 6.859 ± 0.617
2.066ValPhe: 2.066 ± 0.404
6.694ValGly: 6.694 ± 1.028
0.992ValHis: 0.992 ± 0.353
2.562ValIle: 2.562 ± 0.432
3.636ValLys: 3.636 ± 0.593
7.851ValLeu: 7.851 ± 0.772
1.322ValMet: 1.322 ± 0.333
1.405ValAsn: 1.405 ± 0.264
5.702ValPro: 5.702 ± 0.693
2.644ValGln: 2.644 ± 0.522
7.603ValArg: 7.603 ± 0.716
4.38ValSer: 4.38 ± 0.764
5.867ValThr: 5.867 ± 0.624
8.264ValVal: 8.264 ± 1.042
1.653ValTrp: 1.653 ± 0.358
1.983ValTyr: 1.983 ± 0.373
0.0ValXaa: 0.0 ± 0.0
Trp
2.314TrpAla: 2.314 ± 0.424
0.165TrpCys: 0.165 ± 0.116
1.322TrpAsp: 1.322 ± 0.334
0.661TrpGlu: 0.661 ± 0.221
1.074TrpPhe: 1.074 ± 0.325
1.24TrpGly: 1.24 ± 0.363
0.496TrpHis: 0.496 ± 0.182
0.661TrpIle: 0.661 ± 0.309
0.661TrpLys: 0.661 ± 0.226
1.818TrpLeu: 1.818 ± 0.401
0.248TrpMet: 0.248 ± 0.12
0.744TrpAsn: 0.744 ± 0.252
1.074TrpPro: 1.074 ± 0.298
0.992TrpGln: 0.992 ± 0.325
1.818TrpArg: 1.818 ± 0.389
1.322TrpSer: 1.322 ± 0.255
1.405TrpThr: 1.405 ± 0.28
2.231TrpVal: 2.231 ± 0.424
0.578TrpTrp: 0.578 ± 0.231
0.331TrpTyr: 0.331 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.983TyrAla: 1.983 ± 0.432
0.331TyrCys: 0.331 ± 0.159
1.653TyrAsp: 1.653 ± 0.242
1.322TyrGlu: 1.322 ± 0.32
0.578TyrPhe: 0.578 ± 0.198
1.653TyrGly: 1.653 ± 0.446
0.165TyrHis: 0.165 ± 0.103
0.165TyrIle: 0.165 ± 0.104
0.496TyrLys: 0.496 ± 0.206
2.892TyrLeu: 2.892 ± 0.446
0.496TyrMet: 0.496 ± 0.238
0.496TyrAsn: 0.496 ± 0.238
0.578TyrPro: 0.578 ± 0.224
0.165TyrGln: 0.165 ± 0.112
2.479TyrArg: 2.479 ± 0.407
1.653TyrSer: 1.653 ± 0.44
0.496TyrThr: 0.496 ± 0.251
1.57TyrVal: 1.57 ± 0.273
0.165TyrTrp: 0.165 ± 0.106
0.165TyrTyr: 0.165 ± 0.109
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (12102 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski