Sutterella sp. AM11-39
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1912 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A415CFK7|A0A415CFK7_9BURK TetR/AcrR family transcriptional regulator OS=Sutterella sp. AM11-39 OX=2292075 GN=DW133_05565 PE=4 SV=1
MM1 pKa = 7.79 IYY3 pKa = 10.13 FYY5 pKa = 10.52 WLTGLGMAILLSVVVSTTLDD25 pKa = 3.25 NRR27 pKa = 11.84 EE28 pKa = 4.21 AADD31 pKa = 3.58 EE32 pKa = 4.11
Molecular weight: 3.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.871
IPC2_protein 4.113
IPC_protein 3.694
Toseland 3.579
ProMoST 3.821
Dawson 3.732
Bjellqvist 4.151
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.528
Solomon 3.63
Lehninger 3.592
Nozaki 3.935
DTASelect 3.986
Thurlkill 3.706
EMBOSS 3.732
Sillero 3.846
Patrickios 1.901
IPC_peptide 3.63
IPC2_peptide 3.795
IPC2.peptide.svr19 3.921
Protein with the highest isoelectric point:
>tr|A0A415CFR0|A0A415CFR0_9BURK 16S rRNA m5C967 methyltransferase OS=Sutterella sp. AM11-39 OX=2292075 GN=DW133_05960 PE=3 SV=1
MM1 pKa = 6.85 ATKK4 pKa = 9.51 RR5 pKa = 11.84 TYY7 pKa = 10.41 QPSKK11 pKa = 7.96 TKK13 pKa = 10.33 RR14 pKa = 11.84 ARR16 pKa = 11.84 THH18 pKa = 5.82 GFLVRR23 pKa = 11.84 SRR25 pKa = 11.84 TKK27 pKa = 10.32 GGRR30 pKa = 11.84 AVLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.37 GRR41 pKa = 11.84 HH42 pKa = 3.98 VLALL46 pKa = 3.77
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1912
0
1912
646839
21
5244
338.3
37.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.629 ± 0.067
1.373 ± 0.029
5.35 ± 0.042
6.29 ± 0.056
4.07 ± 0.04
7.593 ± 0.059
1.869 ± 0.023
5.457 ± 0.037
5.009 ± 0.045
9.687 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.651 ± 0.027
3.316 ± 0.042
4.315 ± 0.049
3.307 ± 0.035
6.005 ± 0.058
5.875 ± 0.056
5.591 ± 0.05
7.853 ± 0.041
1.241 ± 0.021
2.52 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here