Sphingobium fluviale

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2932 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q1KID1|A0A4Q1KID1_9SPHN NAD(P)H-quinone oxidoreductase OS=Sphingobium fluviale OX=2506423 GN=EQG66_08315 PE=4 SV=1
MM1 pKa = 7.66AIEE4 pKa = 4.35PNSPLFQAILAMDD17 pKa = 4.27SYY19 pKa = 11.86SRR21 pKa = 11.84GYY23 pKa = 10.82LSGVDD28 pKa = 3.31LRR30 pKa = 11.84LRR32 pKa = 11.84EE33 pKa = 4.21EE34 pKa = 4.17NGNLVVDD41 pKa = 4.46EE42 pKa = 4.88NGAPIPSDD50 pKa = 3.46TIGNTLGHH58 pKa = 6.23LTIIANSSEE67 pKa = 4.21AFPSGDD73 pKa = 3.4DD74 pKa = 3.51QKK76 pKa = 11.87AGFYY80 pKa = 10.47AIAYY84 pKa = 8.62QYY86 pKa = 10.99TDD88 pKa = 3.62ANSQIKK94 pKa = 9.1TIISYY99 pKa = 10.61RR100 pKa = 11.84GTDD103 pKa = 3.42QFFTTYY109 pKa = 10.74DD110 pKa = 3.33ANNNPLVGGDD120 pKa = 3.17IANAYY125 pKa = 9.91LVGSGAAEE133 pKa = 3.68QTQARR138 pKa = 11.84LAIEE142 pKa = 5.02FYY144 pKa = 10.76KK145 pKa = 10.9SVAAAINPQNPDD157 pKa = 3.02PYY159 pKa = 10.49AANIVVTGHH168 pKa = 5.98SSGGGLAGLVGAIYY182 pKa = 10.14HH183 pKa = 6.19KK184 pKa = 10.71EE185 pKa = 3.71GVVFDD190 pKa = 3.78NMAFVEE196 pKa = 4.3AARR199 pKa = 11.84NLSFYY204 pKa = 10.44NGSPSSPNDD213 pKa = 3.28PSDD216 pKa = 3.79LLRR219 pKa = 11.84PLYY222 pKa = 10.94SEE224 pKa = 6.07DD225 pKa = 4.07FYY227 pKa = 11.9QLINGTNGPVLIDD240 pKa = 3.67GNGQRR245 pKa = 11.84SLDD248 pKa = 3.85PRR250 pKa = 11.84WQSGGDD256 pKa = 3.64FYY258 pKa = 11.97AKK260 pKa = 10.57FEE262 pKa = 4.08GLKK265 pKa = 10.14AYY267 pKa = 8.7TIAGEE272 pKa = 3.94ILAEE276 pKa = 4.09DD277 pKa = 5.36FIPGGNAHH285 pKa = 6.59QDD287 pKa = 3.2PVKK290 pKa = 10.33PVEE293 pKa = 4.28LSLAADD299 pKa = 3.54VGWFNLIGDD308 pKa = 3.98NGIDD312 pKa = 3.7PFDD315 pKa = 3.63AHH317 pKa = 6.52NVATLVIRR325 pKa = 11.84MFAGITPAEE334 pKa = 4.05EE335 pKa = 3.87AAFGHH340 pKa = 6.3AWKK343 pKa = 10.2IAAPHH348 pKa = 6.38FWPVMYY354 pKa = 10.32DD355 pKa = 3.38NQFSQSIGVGVADD368 pKa = 4.34GGRR371 pKa = 11.84YY372 pKa = 8.43IAEE375 pKa = 4.61NGPHH379 pKa = 7.03GYY381 pKa = 10.42DD382 pKa = 2.78KK383 pKa = 11.32TMRR386 pKa = 11.84TYY388 pKa = 10.47IAYY391 pKa = 10.18SAIDD395 pKa = 3.64NGADD399 pKa = 3.47DD400 pKa = 5.39LSARR404 pKa = 11.84PYY406 pKa = 10.82GDD408 pKa = 3.11TAIRR412 pKa = 11.84ALYY415 pKa = 10.53DD416 pKa = 3.63DD417 pKa = 5.32ASDD420 pKa = 4.12LGVAMGVASKK430 pKa = 10.72GAAVLRR436 pKa = 11.84YY437 pKa = 9.64AEE439 pKa = 4.1QLSKK443 pKa = 11.41VFVEE447 pKa = 5.87FAAKK451 pKa = 10.14LAMEE455 pKa = 4.79KK456 pKa = 10.36VLQSSDD462 pKa = 3.16YY463 pKa = 9.46ATEE466 pKa = 4.06HH467 pKa = 6.57NALQGILSLTEE478 pKa = 4.09GNTGITADD486 pKa = 4.13FSSAKK491 pKa = 9.1WQFDD495 pKa = 3.26NEE497 pKa = 4.22APSNIISKK505 pKa = 10.1QLLLGNLRR513 pKa = 11.84IDD515 pKa = 4.65DD516 pKa = 4.15LQKK519 pKa = 11.01QYY521 pKa = 11.33ILDD524 pKa = 3.79QFEE527 pKa = 5.21HH528 pKa = 5.82IQKK531 pKa = 8.38ITISTTRR538 pKa = 11.84DD539 pKa = 2.8AFNYY543 pKa = 8.35VIPASGGNSTQMFIEE558 pKa = 4.33NSTSEE563 pKa = 4.46STSGAQIVVGGAGNDD578 pKa = 3.1AVYY581 pKa = 11.04ARR583 pKa = 11.84IGGDD587 pKa = 3.44FVIGGHH593 pKa = 6.99GIDD596 pKa = 3.38TVDD599 pKa = 3.16YY600 pKa = 9.59SGIYY604 pKa = 8.84IAPPEE609 pKa = 4.31GEE611 pKa = 4.73IYY613 pKa = 9.37PAPNYY618 pKa = 10.41DD619 pKa = 3.61PYY621 pKa = 11.6SYY623 pKa = 8.08TTGLEE628 pKa = 3.97ADD630 pKa = 5.17FSAAAEE636 pKa = 4.37SGFGTVKK643 pKa = 10.76LMGSMHH649 pKa = 5.5TVKK652 pKa = 10.59DD653 pKa = 3.55YY654 pKa = 11.37LFSVEE659 pKa = 5.26HH660 pKa = 6.08LTLTDD665 pKa = 3.61YY666 pKa = 11.76DD667 pKa = 4.14DD668 pKa = 5.29GLWLSTPVAGVEE680 pKa = 3.74RR681 pKa = 11.84WFDD684 pKa = 3.26GGAGGDD690 pKa = 3.52TVFYY694 pKa = 8.89DD695 pKa = 3.72TSNLIRR701 pKa = 11.84DD702 pKa = 4.07DD703 pKa = 4.12VNSVVQNINGTGKK716 pKa = 10.16DD717 pKa = 3.14HH718 pKa = 7.07LLNVDD723 pKa = 4.71FFGFSPSQYY732 pKa = 9.99YY733 pKa = 10.66DD734 pKa = 2.93IVNGNLPGPVLNMAAKK750 pKa = 10.26YY751 pKa = 9.69IIPDD755 pKa = 2.94VRR757 pKa = 11.84YY758 pKa = 7.74TYY760 pKa = 10.99DD761 pKa = 3.24VNASSASTLDD771 pKa = 3.62YY772 pKa = 10.23STSAANILFTLGVQYY787 pKa = 9.46NTAEE791 pKa = 4.16MFDD794 pKa = 3.35ASGVHH799 pKa = 4.98THH801 pKa = 7.43LISEE805 pKa = 4.5TPSMVIGSNAVGNTISFYY823 pKa = 10.42GTGAFTGGIQDD834 pKa = 3.79DD835 pKa = 4.64TIVIQTGGFEE845 pKa = 4.23TMGRR849 pKa = 11.84ITIGYY854 pKa = 8.42QGGYY858 pKa = 9.04DD859 pKa = 3.42QIVSYY864 pKa = 10.8GSEE867 pKa = 4.18LAPGLNPVNAPLLILMANGILPDD890 pKa = 3.56QVSVEE895 pKa = 4.09FFNQSEE901 pKa = 4.21QDD903 pKa = 3.28GDD905 pKa = 4.24PLVGTYY911 pKa = 9.98FPVTYY916 pKa = 10.4ADD918 pKa = 3.85ALITVDD924 pKa = 4.06GYY926 pKa = 10.26GTISIEE932 pKa = 4.52GLKK935 pKa = 10.44LYY937 pKa = 10.9NNGSFVPLQIAFGDD951 pKa = 3.87GTTWEE956 pKa = 4.25WGEE959 pKa = 4.21TGQPASLFDD968 pKa = 3.98YY969 pKa = 7.92DD970 pKa = 3.66TSFFGKK976 pKa = 10.64GNWDD980 pKa = 3.52NNVLTGYY987 pKa = 9.79EE988 pKa = 4.23GQDD991 pKa = 3.25NTLHH995 pKa = 6.66GFGGDD1000 pKa = 3.49DD1001 pKa = 4.26TIIASYY1007 pKa = 11.36GNDD1010 pKa = 3.17LVYY1013 pKa = 10.97GGDD1016 pKa = 3.69GEE1018 pKa = 5.13DD1019 pKa = 5.12FIDD1022 pKa = 5.02AGMGVNTVYY1031 pKa = 10.95GGFGADD1037 pKa = 3.02TVVMSPYY1044 pKa = 10.63SVTNFVDD1051 pKa = 3.43YY1052 pKa = 9.15TAAQKK1057 pKa = 10.67DD1058 pKa = 4.34IIILPYY1064 pKa = 8.81TVSDD1068 pKa = 3.59ISQLVTSWWTSPHH1081 pKa = 6.06GAQGLEE1087 pKa = 4.12LYY1089 pKa = 10.83ADD1091 pKa = 3.92NTSMLLGIEE1100 pKa = 4.03RR1101 pKa = 11.84PEE1103 pKa = 5.33AEE1105 pKa = 5.16DD1106 pKa = 3.08EE1107 pKa = 4.34DD1108 pKa = 4.41AVVRR1112 pKa = 11.84VQLNSGTKK1120 pKa = 10.26LSLAVDD1126 pKa = 3.82RR1127 pKa = 11.84DD1128 pKa = 3.8GLITSVTSIGSGGDD1142 pKa = 3.14DD1143 pKa = 3.58DD1144 pKa = 6.29GIYY1147 pKa = 8.25GTPDD1151 pKa = 3.18NDD1153 pKa = 4.32TLHH1156 pKa = 5.38GTAWRR1161 pKa = 11.84DD1162 pKa = 3.67FIYY1165 pKa = 10.82GLAGDD1170 pKa = 4.13DD1171 pKa = 4.5VIWGYY1176 pKa = 11.78ADD1178 pKa = 4.65DD1179 pKa = 4.62DD1180 pKa = 5.21VIVGGAGDD1188 pKa = 3.63DD1189 pKa = 3.63TLYY1192 pKa = 11.4GGVGNDD1198 pKa = 3.34NYY1200 pKa = 10.49WFNLGDD1206 pKa = 3.92GSDD1209 pKa = 3.96TIVDD1213 pKa = 3.62EE1214 pKa = 4.92GGSLDD1219 pKa = 4.09RR1220 pKa = 11.84IRR1222 pKa = 11.84FAASVEE1228 pKa = 4.3SSSVSYY1234 pKa = 10.79YY1235 pKa = 10.59QDD1237 pKa = 3.27GDD1239 pKa = 3.99DD1240 pKa = 4.3LFIRR1244 pKa = 11.84YY1245 pKa = 8.09GAGSDD1250 pKa = 3.84EE1251 pKa = 4.05ITVTGFFASDD1261 pKa = 3.11EE1262 pKa = 4.27TRR1264 pKa = 11.84IEE1266 pKa = 3.87EE1267 pKa = 4.19VAFMDD1272 pKa = 4.83GTIHH1276 pKa = 7.2DD1277 pKa = 4.06AAYY1280 pKa = 9.86ILAAVSTGTGTITGTSGADD1299 pKa = 3.27LLEE1302 pKa = 4.49GTEE1305 pKa = 4.06AADD1308 pKa = 3.43ILRR1311 pKa = 11.84GLGGADD1317 pKa = 2.98TLYY1320 pKa = 10.91GYY1322 pKa = 10.78DD1323 pKa = 4.53GDD1325 pKa = 5.84DD1326 pKa = 3.51ILIGGNGHH1334 pKa = 7.05DD1335 pKa = 4.2SLYY1338 pKa = 11.25GGIGNDD1344 pKa = 3.08ILRR1347 pKa = 11.84GGSGNDD1353 pKa = 3.6LLIGGAGNDD1362 pKa = 3.47ILKK1365 pKa = 10.74GSAGNDD1371 pKa = 2.98ILRR1374 pKa = 11.84GGSGDD1379 pKa = 4.15DD1380 pKa = 3.27ILRR1383 pKa = 11.84GGDD1386 pKa = 3.33GDD1388 pKa = 4.0DD1389 pKa = 3.39RR1390 pKa = 11.84LVGGRR1395 pKa = 11.84GSDD1398 pKa = 3.4TLDD1401 pKa = 3.49GGNGVDD1407 pKa = 3.23TFVFRR1412 pKa = 11.84ANHH1415 pKa = 6.3IGTGVDD1421 pKa = 3.61TIKK1424 pKa = 11.02DD1425 pKa = 3.46FSLAQNDD1432 pKa = 4.6KK1433 pKa = 10.86IDD1435 pKa = 3.84ISDD1438 pKa = 4.31LLSAYY1443 pKa = 10.27DD1444 pKa = 4.13PLTDD1448 pKa = 3.76ALADD1452 pKa = 3.95FVQFTRR1458 pKa = 11.84SGKK1461 pKa = 8.99KK1462 pKa = 9.86SNMFVDD1468 pKa = 4.23LDD1470 pKa = 4.04GAGAEE1475 pKa = 4.3HH1476 pKa = 7.29DD1477 pKa = 3.87WVHH1480 pKa = 5.63VATLQGVTALPDD1492 pKa = 3.25VDD1494 pKa = 3.67TLVANGHH1501 pKa = 6.54LLAAA1505 pKa = 5.26

Molecular weight: 159.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q1KGR2|A0A4Q1KGR2_9SPHN UvrABC system protein C OS=Sphingobium fluviale OX=2506423 GN=uvrC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84NILRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2932

0

2932

915520

23

6626

312.3

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.108 ± 0.066

0.873 ± 0.017

5.874 ± 0.055

5.559 ± 0.048

3.405 ± 0.032

8.755 ± 0.104

2.073 ± 0.026

5.364 ± 0.028

3.296 ± 0.041

9.917 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.026

2.659 ± 0.035

5.074 ± 0.044

3.206 ± 0.027

7.082 ± 0.063

5.527 ± 0.047

5.114 ± 0.051

6.908 ± 0.037

1.366 ± 0.019

2.223 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski