Edaphobacter modestus
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6023 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q7YXZ5|A0A4Q7YXZ5_9BACT LytTR family two component transcriptional regulator OS=Edaphobacter modestus OX=388466 GN=BDD14_3899 PE=4 SV=1
MM1 pKa = 7.47 ANIIEE6 pKa = 4.96 GYY8 pKa = 7.76 WLSVVTSDD16 pKa = 4.31 FFVINFTTDD25 pKa = 3.46 PFPPGTSLYY34 pKa = 11.15 ANISLSEE41 pKa = 4.03 INTLFSGNNPNDD53 pKa = 3.41 PTFAATAFIDD63 pKa = 3.16 SWTVYY68 pKa = 10.32 LADD71 pKa = 3.99 GTEE74 pKa = 4.01 STPIQGQGFAQNAIGLDD91 pKa = 3.22 NCARR95 pKa = 11.84 IHH97 pKa = 6.04 FVLVGDD103 pKa = 4.41 RR104 pKa = 11.84 VAAIAQVNIFRR115 pKa = 4.8
Molecular weight: 12.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.961
IPC_protein 3.859
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.795
Nozaki 3.999
DTASelect 4.24
Thurlkill 3.732
EMBOSS 3.846
Sillero 3.986
Patrickios 1.926
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.871
Protein with the highest isoelectric point:
>tr|A0A4Q7YVV8|A0A4Q7YVV8_9BACT Uncharacterized protein DUF4339 OS=Edaphobacter modestus OX=388466 GN=BDD14_2812 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.02 RR4 pKa = 11.84 TFQPNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SKK15 pKa = 8.65 THH17 pKa = 5.87 GFLTRR22 pKa = 11.84 MKK24 pKa = 9.03 TKK26 pKa = 10.72 AGAAVLNRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.03 GRR40 pKa = 11.84 HH41 pKa = 5.72 KK42 pKa = 10.29 IAVSAGFRR50 pKa = 11.84 DD51 pKa = 3.66
Molecular weight: 5.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.369
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.087
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6023
0
6023
2031366
28
2469
337.3
36.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.316 ± 0.034
0.9 ± 0.009
5.054 ± 0.022
5.385 ± 0.032
3.976 ± 0.025
7.923 ± 0.027
2.269 ± 0.014
5.177 ± 0.022
3.713 ± 0.025
9.889 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.248 ± 0.017
3.518 ± 0.035
5.275 ± 0.021
3.857 ± 0.022
6.421 ± 0.032
6.724 ± 0.029
6.096 ± 0.032
7.126 ± 0.023
1.397 ± 0.012
2.734 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here