Hyphomonas neptunium (strain ATCC 15444)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomonadales; Hyphomonadaceae; Hyphomonas; Hyphomonas neptunium

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3499 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0C3X7|Q0C3X7_HYPNA Fumarylacetoacetate hydrolase family protein OS=Hyphomonas neptunium (strain ATCC 15444) OX=228405 GN=HNE_0839 PE=4 SV=1
MM1 pKa = 7.32TVLTLLGAAVLPAHH15 pKa = 7.06ADD17 pKa = 3.48DD18 pKa = 5.21SRR20 pKa = 11.84LLTSATLADD29 pKa = 3.84LQSIVVEE36 pKa = 4.11EE37 pKa = 5.56GYY39 pKa = 9.44TILSTGNDD47 pKa = 3.6GEE49 pKa = 4.37VSVRR53 pKa = 11.84AKK55 pKa = 9.54TAEE58 pKa = 3.72GLVFNVIGTVCDD70 pKa = 3.57SEE72 pKa = 4.86NADD75 pKa = 3.35GCLGINMQVRR85 pKa = 11.84YY86 pKa = 9.72DD87 pKa = 3.74ADD89 pKa = 3.49GKK91 pKa = 9.33EE92 pKa = 4.05TLEE95 pKa = 5.23RR96 pKa = 11.84INDD99 pKa = 3.77VNLMWAATSAWYY111 pKa = 10.3SVGGTDD117 pKa = 4.73GKK119 pKa = 9.24TPTVGITRR127 pKa = 11.84YY128 pKa = 10.29VILDD132 pKa = 3.81RR133 pKa = 11.84GATIGNIKK141 pKa = 10.71DD142 pKa = 3.4NLLNVLAIAPNAANYY157 pKa = 8.51IWQAGEE163 pKa = 3.75YY164 pKa = 9.7APGYY168 pKa = 7.81EE169 pKa = 5.36AEE171 pKa = 4.37YY172 pKa = 10.81EE173 pKa = 3.91DD174 pKa = 5.76DD175 pKa = 3.6EE176 pKa = 5.91DD177 pKa = 5.03YY178 pKa = 10.89FYY180 pKa = 11.41EE181 pKa = 4.32DD182 pKa = 2.78WW183 pKa = 5.13

Molecular weight: 19.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0C5A9|Q0C5A9_HYPNA S-methyl-5'-thioadenosine phosphorylase OS=Hyphomonas neptunium (strain ATCC 15444) OX=228405 GN=mtaP PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84EE20 pKa = 4.01RR21 pKa = 11.84MSTKK25 pKa = 10.23NGRR28 pKa = 11.84KK29 pKa = 8.58VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84KK40 pKa = 6.8TLTAA44 pKa = 4.25

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3499

0

3499

1112553

30

2085

318.0

34.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.732 ± 0.06

0.831 ± 0.013

5.716 ± 0.032

6.198 ± 0.036

3.764 ± 0.029

8.749 ± 0.034

1.837 ± 0.02

5.192 ± 0.03

3.398 ± 0.034

9.797 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.57 ± 0.021

2.613 ± 0.024

5.34 ± 0.03

3.059 ± 0.022

6.662 ± 0.037

5.591 ± 0.031

5.385 ± 0.025

6.864 ± 0.036

1.379 ± 0.017

2.323 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski