Moorea producens 3L
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7382 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F4XWZ3|F4XWZ3_9CYAN Uncharacterized protein OS=Moorea producens 3L OX=489825 GN=LYNGBM3L_46340 PE=4 SV=1
MM1 pKa = 7.72 IDD3 pKa = 4.41 IKK5 pKa = 9.88 TSWVSYY11 pKa = 10.45 QLAFLFNSFRR21 pKa = 11.84 PVMKK25 pKa = 10.65 SLFNYY30 pKa = 9.58 LKK32 pKa = 10.38 KK33 pKa = 10.43 IPNHH37 pKa = 6.35 SSDD40 pKa = 3.38 SPRR43 pKa = 11.84 ASEE46 pKa = 5.45 VNSDD50 pKa = 3.39 WQPLGFNLMGITNLAKK66 pKa = 9.62 ATFPILVTGSALLVSTPTEE85 pKa = 3.67 VAGQVGIDD93 pKa = 3.43 ASLATGPRR101 pKa = 11.84 PFSLRR106 pKa = 11.84 YY107 pKa = 8.75 SAHH110 pKa = 5.7 VPGNMVAIGNASLICDD126 pKa = 3.7 RR127 pKa = 11.84 TNASCLNGLATGNVGNNSGGLAMQMLDD154 pKa = 3.65 SDD156 pKa = 4.65 SDD158 pKa = 3.75 PSTFNSSSADD168 pKa = 3.19 LTLPVGANVLFAGLYY183 pKa = 8.9 WGGTSDD189 pKa = 4.99 GATTPAPDD197 pKa = 3.18 ASKK200 pKa = 10.96 RR201 pKa = 11.84 NEE203 pKa = 4.02 ALLATPEE210 pKa = 4.23 GGYY213 pKa = 7.73 QTVTADD219 pKa = 3.32 TFTSIDD225 pKa = 3.41 NSATSGWDD233 pKa = 3.39 VYY235 pKa = 11.72 SSFADD240 pKa = 3.51 VTSLVQAAGSGTYY253 pKa = 9.39 TLANVQASTGTGFTYY268 pKa = 10.64 PNAGWSLIVVYY279 pKa = 9.83 EE280 pKa = 4.42 DD281 pKa = 3.3 PSEE284 pKa = 3.92 PRR286 pKa = 11.84 RR287 pKa = 11.84 NMTVFDD293 pKa = 4.97 GYY295 pKa = 11.3 DD296 pKa = 3.16 FSGFNTGNTQTLTGLRR312 pKa = 11.84 TPPTPGFSVFMGAFAGDD329 pKa = 4.07 GEE331 pKa = 4.83 PDD333 pKa = 3.31 VSGDD337 pKa = 3.39 TLSINNTPVSDD348 pKa = 4.0 PVNPLNNFYY357 pKa = 11.1 NSTISQYY364 pKa = 10.68 GSHH367 pKa = 5.23 VTSRR371 pKa = 11.84 TPNDD375 pKa = 4.03 PYY377 pKa = 11.91 NMVVDD382 pKa = 4.72 IDD384 pKa = 4.92 LLDD387 pKa = 3.51 LTAWNEE393 pKa = 3.94 ANNVIPTNATSIDD406 pKa = 4.26 LNLSTSGDD414 pKa = 4.32 GIWPMVYY421 pKa = 10.14 FFGVEE426 pKa = 3.91 VFEE429 pKa = 5.06 PNLVTEE435 pKa = 4.82 FEE437 pKa = 4.38 KK438 pKa = 8.75 TTPQTTYY445 pKa = 11.24 QNGDD449 pKa = 3.65 TIGYY453 pKa = 6.55 TISVTNTGNDD463 pKa = 3.17 NAVNTVITDD472 pKa = 4.23 IIPTGTTFVPGSLKK486 pKa = 10.63 INGVTKK492 pKa = 10.45 TDD494 pKa = 3.36 GATDD498 pKa = 3.38 EE499 pKa = 5.37 AEE501 pKa = 3.95 FDD503 pKa = 3.57 GTNVIFRR510 pKa = 11.84 VGTGANTTQGGQLNVNEE527 pKa = 4.33 TVTMSFEE534 pKa = 4.39 VTVTAAPGEE543 pKa = 4.46 KK544 pKa = 9.9 VCNQASIDD552 pKa = 3.96 YY553 pKa = 9.95 EE554 pKa = 4.62 GEE556 pKa = 3.79 ASGNQASGTSDD567 pKa = 3.86 DD568 pKa = 4.73 PNTATFGDD576 pKa = 4.26 CTEE579 pKa = 4.27 ITVTPPTTTVDD590 pKa = 3.44 YY591 pKa = 11.31 GDD593 pKa = 5.06 APDD596 pKa = 3.98 TTASTGNGDD605 pKa = 4.08 YY606 pKa = 8.84 QTLSANSGPTHH617 pKa = 6.94 TIDD620 pKa = 2.97 NSTYY624 pKa = 10.41 LGAGVTADD632 pKa = 3.42 TDD634 pKa = 3.93 GFGDD638 pKa = 4.05 GTDD641 pKa = 3.7 NNNNATDD648 pKa = 3.78 DD649 pKa = 3.95 TDD651 pKa = 5.86 DD652 pKa = 3.85 GVQINSASLQGQTLPISDD670 pKa = 4.2 NVRR673 pKa = 11.84 LDD675 pKa = 2.93 ITTAGSGVLNAWIDD689 pKa = 3.48 WNADD693 pKa = 2.73 GDD695 pKa = 4.09 FDD697 pKa = 6.37 DD698 pKa = 5.72 SGEE701 pKa = 4.15 QIATNASPSGNAIALNLTVPAGATVGNTYY730 pKa = 11.09 ARR732 pKa = 11.84 FRR734 pKa = 11.84 YY735 pKa = 10.26 SSDD738 pKa = 2.95 TGLTPTGAASDD749 pKa = 4.58 GEE751 pKa = 4.38 VEE753 pKa = 4.81 DD754 pKa = 4.05 YY755 pKa = 10.98 QIAIANSTPTTASVGDD771 pKa = 4.1 QVWEE775 pKa = 4.36 DD776 pKa = 3.63 LDD778 pKa = 5.03 GDD780 pKa = 4.87 GIQDD784 pKa = 3.73 PGEE787 pKa = 4.15 PGIPNALVNIYY798 pKa = 10.5 KK799 pKa = 10.13 SDD801 pKa = 3.65 NTLVDD806 pKa = 3.31 ATFTDD811 pKa = 3.26 SSGNYY816 pKa = 9.44 SFSDD820 pKa = 4.57 LDD822 pKa = 3.96 PGDD825 pKa = 4.27 YY826 pKa = 9.96 IIEE829 pKa = 4.32 FTSPAVGYY837 pKa = 10.7 SFTRR841 pKa = 11.84 QDD843 pKa = 4.02 AGDD846 pKa = 3.94 DD847 pKa = 4.02 TLDD850 pKa = 3.66 SDD852 pKa = 4.71 ADD854 pKa = 3.86 PVTGRR859 pKa = 11.84 TDD861 pKa = 3.38 TFTLAAGDD869 pKa = 4.03 NNTSIDD875 pKa = 3.43 AGLTLKK881 pKa = 11.03 ANTIVACDD889 pKa = 4.66 IIPLQATNWTEE900 pKa = 3.81 TLNVEE905 pKa = 4.61 KK906 pKa = 10.27 FDD908 pKa = 4.09 SALGSLARR916 pKa = 11.84 VDD918 pKa = 4.06 LTLSTLIAQEE928 pKa = 4.62 LIYY931 pKa = 10.68 EE932 pKa = 4.28 NTADD936 pKa = 3.65 QPKK939 pKa = 9.65 TITVNQEE946 pKa = 3.74 GEE948 pKa = 4.47 VSVSLPDD955 pKa = 3.37 TNANITRR962 pKa = 11.84 NYY964 pKa = 9.1 NQSTDD969 pKa = 3.77 FNLPAFDD976 pKa = 5.29 GSDD979 pKa = 3.43 PPDD982 pKa = 3.59 FSGTSGGRR990 pKa = 11.84 TPTILAYY997 pKa = 7.55 EE998 pKa = 4.31 THH1000 pKa = 5.92 TEE1002 pKa = 4.19 EE1003 pKa = 4.38 YY1004 pKa = 10.12 TNLADD1009 pKa = 5.89 FISSSSPVEE1018 pKa = 4.1 TVSIPVNTTSAATFINSQNASTGSSSEE1045 pKa = 3.97 ATAGLCVTYY1054 pKa = 9.52 TYY1056 pKa = 11.42 SLVPKK1061 pKa = 9.1 EE1062 pKa = 4.26 PSASIVPEE1070 pKa = 3.94 EE1071 pKa = 4.41 ASPEE1075 pKa = 3.91 AGNIVINEE1083 pKa = 3.79 VLYY1086 pKa = 10.92 AQTGSSAEE1094 pKa = 4.07 ANDD1097 pKa = 4.05 EE1098 pKa = 4.56 FIEE1101 pKa = 5.29 LYY1103 pKa = 10.57 NSSDD1107 pKa = 3.54 NAVDD1111 pKa = 3.46 ISNWKK1116 pKa = 9.64 LADD1119 pKa = 3.47 SNLIFNSTDD1128 pKa = 2.82 NTGNITGDD1136 pKa = 3.64 AANPAYY1142 pKa = 9.76 IFPNNTTLQPGEE1154 pKa = 4.32 YY1155 pKa = 10.02 AVIWIGNNTSDD1166 pKa = 4.49 HH1167 pKa = 5.81 QAPDD1171 pKa = 3.19 AAFQDD1176 pKa = 4.13 WLGKK1180 pKa = 9.48 AAKK1183 pKa = 9.84 LNNAGEE1189 pKa = 4.87 DD1190 pKa = 2.55 IWLYY1194 pKa = 11.06 DD1195 pKa = 3.9 EE1196 pKa = 4.43 EE1197 pKa = 4.79 FKK1199 pKa = 11.0 IVDD1202 pKa = 3.7 YY1203 pKa = 10.78 IAYY1206 pKa = 9.9 GSNNAINTPPSAEE1219 pKa = 4.01 LNLWDD1224 pKa = 4.63 DD1225 pKa = 4.34 TYY1227 pKa = 11.71 QRR1229 pKa = 11.84 DD1230 pKa = 3.78 LDD1232 pKa = 4.11 GASTGQSISLTPNGEE1247 pKa = 4.32 DD1248 pKa = 4.54 GNTSACWEE1256 pKa = 4.23 HH1257 pKa = 6.26 TLSNDD1262 pKa = 3.09 ANGRR1266 pKa = 11.84 CPEE1269 pKa = 3.97 FLEE1272 pKa = 4.56 TRR1274 pKa = 11.84 DD1275 pKa = 3.37 TDD1277 pKa = 4.08 TVSVRR1282 pKa = 11.84 QTSVGVYY1289 pKa = 10.77 NNILPNVILVKK1300 pKa = 10.42 RR1301 pKa = 11.84 ITAIKK1306 pKa = 10.17 PKK1308 pKa = 9.52 DD1309 pKa = 3.5 TNQWIEE1315 pKa = 4.13 LPQSGSPFIDD1325 pKa = 5.18 GIDD1328 pKa = 3.78 GGSSEE1333 pKa = 4.89 NNVGSDD1339 pKa = 4.01 RR1340 pKa = 11.84 AADD1343 pKa = 4.0 DD1344 pKa = 5.33 NDD1346 pKa = 4.52 PNWPNPDD1353 pKa = 2.87 VYY1355 pKa = 11.19 LRR1357 pKa = 11.84 GLINSGQVMPGDD1369 pKa = 3.64 EE1370 pKa = 4.71 LEE1372 pKa = 4.23 YY1373 pKa = 10.4 TVYY1376 pKa = 10.58 FLSNGSNDD1384 pKa = 3.3 AKK1386 pKa = 10.8 NLRR1389 pKa = 11.84 ICDD1392 pKa = 3.85 RR1393 pKa = 11.84 VPGEE1397 pKa = 4.17 TTFITDD1403 pKa = 3.8 SFNATAGFPSSDD1415 pKa = 2.96 VGIAMFQSTTALASGGPAEE1434 pKa = 4.12 PNIYY1438 pKa = 8.7 LTNIPDD1444 pKa = 3.62 SDD1446 pKa = 3.26 RR1447 pKa = 11.84 GRR1449 pKa = 11.84 YY1450 pKa = 8.22 YY1451 pKa = 11.18 GPGTSVPSGCNVTFNQNGVVVVEE1474 pKa = 4.49 VGDD1477 pKa = 3.82 VPNADD1482 pKa = 3.78 SPGNPPNSYY1491 pKa = 10.41 GFIRR1495 pKa = 11.84 FRR1497 pKa = 11.84 ALVKK1501 pKa = 10.72
Molecular weight: 158.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.656
IPC_protein 3.719
Toseland 3.478
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.528
Grimsley 3.389
Solomon 3.719
Lehninger 3.668
Nozaki 3.821
DTASelect 4.101
Thurlkill 3.528
EMBOSS 3.668
Sillero 3.834
Patrickios 1.443
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.773
Protein with the highest isoelectric point:
>tr|F4XIZ2|F4XIZ2_9CYAN Uncharacterized protein OS=Moorea producens 3L OX=489825 GN=LYNGBM3L_04520 PE=4 SV=1
MM1 pKa = 7.84 SIQQSAVSGQRR12 pKa = 11.84 SAVSGQHH19 pKa = 5.67 SAVSGQRR26 pKa = 11.84 SAFSGQRR33 pKa = 11.84 SAVSIQRR40 pKa = 11.84 SAVSGQHH47 pKa = 5.67 SAVSGQRR54 pKa = 11.84 SAFSGQRR61 pKa = 11.84 SAVSIQRR68 pKa = 11.84 SAVSGQHH75 pKa = 5.67 SAVSGQRR82 pKa = 11.84 SAFSGQRR89 pKa = 11.84 SAVSIQRR96 pKa = 11.84 SAVSGQHH103 pKa = 5.67 SAVSGQRR110 pKa = 11.84 SAFSGQRR117 pKa = 11.84 SAVSIQRR124 pKa = 11.84 SAVSGQQTT132 pKa = 3.04
Molecular weight: 13.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.31
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.257
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7382
0
7382
2118520
15
4862
287.0
32.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.434 ± 0.028
1.038 ± 0.012
5.111 ± 0.032
6.201 ± 0.03
3.823 ± 0.022
6.801 ± 0.035
1.861 ± 0.014
6.497 ± 0.028
5.208 ± 0.034
10.921 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.837 ± 0.015
4.4 ± 0.027
4.818 ± 0.025
5.437 ± 0.031
5.123 ± 0.026
6.675 ± 0.024
5.588 ± 0.027
6.491 ± 0.024
1.515 ± 0.013
3.127 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here