Scytonema tolypothrichoides VB-61278
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5627 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C2M242|A0A0C2M242_9CYAN Ankyrin OS=Scytonema tolypothrichoides VB-61278 OX=1233231 GN=SD80_26705 PE=4 SV=1
MM1 pKa = 7.26 ATYY4 pKa = 10.56 KK5 pKa = 9.5 VTLINDD11 pKa = 3.46 AEE13 pKa = 4.92 GINKK17 pKa = 8.82 TIEE20 pKa = 4.13 VPDD23 pKa = 4.14 DD24 pKa = 3.89 EE25 pKa = 5.48 YY26 pKa = 11.12 ILDD29 pKa = 3.65 IAEE32 pKa = 4.68 EE33 pKa = 4.2 EE34 pKa = 4.59 DD35 pKa = 3.87 LDD37 pKa = 4.68 LPYY40 pKa = 10.71 SCRR43 pKa = 11.84 SGSCSDD49 pKa = 3.57 CAGLIKK55 pKa = 10.65 SGTVDD60 pKa = 3.17 QSDD63 pKa = 4.01 GNFLDD68 pKa = 5.78 DD69 pKa = 4.28 DD70 pKa = 4.72 QIDD73 pKa = 3.3 QGYY76 pKa = 9.37 VLTCIAKK83 pKa = 7.64 PTSDD87 pKa = 3.45 VTIEE91 pKa = 3.91 THH93 pKa = 6.75 KK94 pKa = 11.01 KK95 pKa = 10.58 DD96 pKa = 5.07 DD97 pKa = 3.62 IEE99 pKa = 4.06
Molecular weight: 10.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.745
IPC_protein 3.732
Toseland 3.516
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.427
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.101
Thurlkill 3.579
EMBOSS 3.694
Sillero 3.859
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.804
Protein with the highest isoelectric point:
>tr|A0A0C2M4I7|A0A0C2M4I7_9CYAN CopG family transcriptional regulator OS=Scytonema tolypothrichoides VB-61278 OX=1233231 GN=SD80_23470 PE=4 SV=1
MM1 pKa = 6.86 QASLALRR8 pKa = 11.84 NRR10 pKa = 11.84 PSTKK14 pKa = 10.22 LLCIFNAQQQGRR26 pKa = 11.84 RR27 pKa = 11.84 QTAEE31 pKa = 3.59 GRR33 pKa = 11.84 RR34 pKa = 11.84 KK35 pKa = 9.99 EE36 pKa = 4.16 SFNDD40 pKa = 3.62 GLNQNNMQVKK50 pKa = 9.93 SATAYY55 pKa = 9.64 DD56 pKa = 4.07 QGWLAYY62 pKa = 9.49 IRR64 pKa = 11.84 TGG66 pKa = 3.39
Molecular weight: 7.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.341
IPC2_protein 9.736
IPC_protein 10.599
Toseland 10.672
ProMoST 10.394
Dawson 10.774
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.965
Grimsley 10.833
Solomon 10.906
Lehninger 10.877
Nozaki 10.657
DTASelect 10.496
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.701
Patrickios 10.774
IPC_peptide 10.906
IPC2_peptide 9.589
IPC2.peptide.svr19 8.473
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5625
2
5627
1720987
29
2331
305.8
34.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.105 ± 0.034
1.012 ± 0.011
4.728 ± 0.022
6.256 ± 0.03
3.998 ± 0.022
6.752 ± 0.036
1.911 ± 0.019
6.427 ± 0.025
4.928 ± 0.031
10.906 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.87 ± 0.014
4.18 ± 0.026
4.718 ± 0.025
5.358 ± 0.031
5.296 ± 0.028
6.4 ± 0.029
5.722 ± 0.025
7.045 ± 0.027
1.393 ± 0.016
2.994 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here