Candidatus Burkholderia verschuerenii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4492 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0MEF8|A0A0L0MEF8_9BURK Acetyltransferase OS=Candidatus Burkholderia verschuerenii OX=242163 GN=BVER_05466 PE=4 SV=1
MM1 pKa = 8.43SMEE4 pKa = 3.94YY5 pKa = 10.12RR6 pKa = 11.84SWMCLICGWIYY17 pKa = 11.01DD18 pKa = 4.28EE19 pKa = 4.8EE20 pKa = 6.26AGLPDD25 pKa = 3.89EE26 pKa = 5.81GIAPGTRR33 pKa = 11.84WEE35 pKa = 4.49DD36 pKa = 3.51VPINWTCPEE45 pKa = 3.97CGARR49 pKa = 11.84KK50 pKa = 9.54EE51 pKa = 4.07DD52 pKa = 3.87FEE54 pKa = 4.52MVQII58 pKa = 4.59

Molecular weight: 6.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0M9D3|A0A0L0M9D3_9BURK Non-specific DNA-binding protein Dps / Iron-binding ferritin-like antioxidant protein / Ferroxidase OS=Candidatus Burkholderia verschuerenii OX=242163 GN=BVER_05616c PE=3 SV=1
MM1 pKa = 7.33GWLGLIMLGAVIGAAGWWLHH21 pKa = 4.85PLRR24 pKa = 11.84RR25 pKa = 11.84AGRR28 pKa = 11.84VGRR31 pKa = 11.84GAAGRR36 pKa = 11.84IWTAIAAGILATIVALGGRR55 pKa = 11.84RR56 pKa = 3.33

Molecular weight: 5.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4492

0

4492

1394682

38

1971

310.5

33.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.197 ± 0.045

0.947 ± 0.012

5.78 ± 0.03

5.305 ± 0.038

3.778 ± 0.022

7.857 ± 0.033

2.314 ± 0.018

5.082 ± 0.027

3.586 ± 0.032

9.899 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.538 ± 0.017

2.929 ± 0.021

4.878 ± 0.024

3.532 ± 0.026

6.952 ± 0.035

5.763 ± 0.026

5.371 ± 0.024

7.571 ± 0.027

1.305 ± 0.013

2.414 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski