Subtercola sp. Z020

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Subtercola; unclassified Subtercola

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S5W0F2|A0A2S5W0F2_9MICO Sugar ABC transporter substrate-binding protein OS=Subtercola sp. Z020 OX=2080582 GN=C5B96_12865 PE=4 SV=1
MM1 pKa = 7.23NAFGEE6 pKa = 4.62SASMPVTSAAPAPSVTAASVTTGSLAGGTAVSLAGQGLGSVVSVSVGGVPATSVAIDD63 pKa = 3.68GEE65 pKa = 4.77GTVTFVTPPAADD77 pKa = 3.75YY78 pKa = 11.01QPAAAALSLANADD91 pKa = 3.55GTVVDD96 pKa = 4.13TGQTFTYY103 pKa = 9.69TAVTPVDD110 pKa = 3.33QQMQYY115 pKa = 11.66AFAHH119 pKa = 5.44WKK121 pKa = 10.27DD122 pKa = 3.63YY123 pKa = 11.49NLADD127 pKa = 3.18WSVFSDD133 pKa = 3.41NDD135 pKa = 3.38CGNFVNQTLVARR147 pKa = 11.84GWEE150 pKa = 4.19QNDD153 pKa = 3.07DD154 pKa = 3.28WYY156 pKa = 11.78SNYY159 pKa = 10.29GSGGDD164 pKa = 4.15YY165 pKa = 10.07SYY167 pKa = 11.7SWIRR171 pKa = 11.84GNEE174 pKa = 3.57MDD176 pKa = 5.58DD177 pKa = 3.96YY178 pKa = 11.35LDD180 pKa = 4.0SRR182 pKa = 11.84PEE184 pKa = 3.9TTRR187 pKa = 11.84LEE189 pKa = 4.31LSDD192 pKa = 3.8RR193 pKa = 11.84ASLKK197 pKa = 10.64IGDD200 pKa = 3.78VVMFDD205 pKa = 4.2WDD207 pKa = 3.77PQNDD211 pKa = 3.6NGVDD215 pKa = 3.22HH216 pKa = 6.9TMLVSRR222 pKa = 11.84VDD224 pKa = 3.66RR225 pKa = 11.84NADD228 pKa = 3.11GSVSVKK234 pKa = 9.91MVGHH238 pKa = 6.1TVDD241 pKa = 2.99AQYY244 pKa = 11.32RR245 pKa = 11.84DD246 pKa = 4.37LDD248 pKa = 3.8TAITVEE254 pKa = 4.29NPGGTAHH261 pKa = 7.19FYY263 pKa = 11.05SIAA266 pKa = 3.48

Molecular weight: 28.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S5W207|A0A2S5W207_9MICO ABC transporter permease OS=Subtercola sp. Z020 OX=2080582 GN=C5B96_11175 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3250

0

3250

1047158

29

1637

322.2

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.566 ± 0.07

0.472 ± 0.01

5.852 ± 0.031

5.176 ± 0.044

3.263 ± 0.028

9.122 ± 0.04

1.878 ± 0.022

4.547 ± 0.034

2.041 ± 0.029

10.267 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.632 ± 0.014

2.223 ± 0.024

5.479 ± 0.032

2.839 ± 0.024

6.691 ± 0.047

6.389 ± 0.033

6.351 ± 0.045

8.869 ± 0.034

1.325 ± 0.018

2.017 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski