Marinobacter santoriniensis NKSG1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter; Marinobacter santoriniensis

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3692 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|M7CKV5|M7CKV5_9ALTE Histidine kinase OS=Marinobacter santoriniensis NKSG1 OX=1288826 GN=MSNKSG1_16161 PE=4 SV=1
MM1 pKa = 7.15KK2 pKa = 10.33RR3 pKa = 11.84SMQMFAVSALAAGVAACGGGGDD25 pKa = 4.2GSATGSTGTVSFNMTDD41 pKa = 3.23APAMEE46 pKa = 4.46FTDD49 pKa = 3.34VTVAFTGIRR58 pKa = 11.84LKK60 pKa = 11.04HH61 pKa = 6.39EE62 pKa = 4.79DD63 pKa = 3.79GDD65 pKa = 4.2WIEE68 pKa = 4.24FTFDD72 pKa = 4.87QPKK75 pKa = 8.18TWNLLDD81 pKa = 3.93LQGGMHH87 pKa = 6.81EE88 pKa = 4.31PLITDD93 pKa = 3.75EE94 pKa = 4.48EE95 pKa = 5.05VPAGDD100 pKa = 3.7YY101 pKa = 10.69TEE103 pKa = 4.47LRR105 pKa = 11.84LMVDD109 pKa = 3.14TADD112 pKa = 3.67SYY114 pKa = 11.83VIEE117 pKa = 4.47SSQPDD122 pKa = 3.75VEE124 pKa = 4.43KK125 pKa = 10.32TLAVPSGEE133 pKa = 4.13QSGLKK138 pKa = 10.35LKK140 pKa = 11.14GNFVVAADD148 pKa = 3.81TSTDD152 pKa = 3.65FTIDD156 pKa = 3.36FNVAKK161 pKa = 10.52SIVNPQGKK169 pKa = 9.05SLADD173 pKa = 3.58YY174 pKa = 10.53LLKK177 pKa = 10.66PSLRR181 pKa = 11.84LTNTLEE187 pKa = 4.2TGSITGEE194 pKa = 3.48VDD196 pKa = 3.31YY197 pKa = 8.81PTINSTRR204 pKa = 11.84SADD207 pKa = 3.59ADD209 pKa = 3.94LANCSDD215 pKa = 4.07GYY217 pKa = 10.68YY218 pKa = 10.88GSIYY222 pKa = 9.85VYY224 pKa = 10.1QGAGVTPTDD233 pKa = 3.47VNVNTEE239 pKa = 4.76DD240 pKa = 4.09GPLMTIPVKK249 pKa = 10.78DD250 pKa = 4.09DD251 pKa = 4.01NNDD254 pKa = 2.89GMYY257 pKa = 10.16SYY259 pKa = 9.69TAAYY263 pKa = 8.26LTEE266 pKa = 4.31GDD268 pKa = 3.68YY269 pKa = 10.68TLSYY273 pKa = 10.14TCQLDD278 pKa = 4.23DD279 pKa = 5.77NEE281 pKa = 4.67TTDD284 pKa = 3.7DD285 pKa = 3.63TVEE288 pKa = 4.12FEE290 pKa = 4.3GTQTVSVVAGEE301 pKa = 4.07EE302 pKa = 4.15TQADD306 pKa = 4.73SIPLTPP312 pKa = 4.83

Molecular weight:
33.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|M7CRC4|M7CRC4_9ALTE Proton/sodium-glutamate symport protein OS=Marinobacter santoriniensis NKSG1 OX=1288826 GN=MSNKSG1_09968 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.91VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.44GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight:
5.11 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3692

0

3692

1219654

16

2517

330.4

36.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.665 ± 0.042

0.937 ± 0.013

5.994 ± 0.031

6.471 ± 0.04

3.8 ± 0.027

7.853 ± 0.039

2.243 ± 0.023

5.228 ± 0.029

3.675 ± 0.033

10.67 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.548 ± 0.018

3.193 ± 0.024

4.744 ± 0.021

3.94 ± 0.031

6.723 ± 0.039

5.933 ± 0.03

5.196 ± 0.027

7.25 ± 0.036

1.377 ± 0.017

2.561 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski