Marinobacter santoriniensis NKSG1
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3692 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M7CKV5|M7CKV5_9ALTE Histidine kinase OS=Marinobacter santoriniensis NKSG1 OX=1288826 GN=MSNKSG1_16161 PE=4 SV=1
MM1 pKa = 7.15 KK2 pKa = 10.33 RR3 pKa = 11.84 SMQMFAVSALAAGVAACGGGGDD25 pKa = 4.2 GSATGSTGTVSFNMTDD41 pKa = 3.23 APAMEE46 pKa = 4.46 FTDD49 pKa = 3.34 VTVAFTGIRR58 pKa = 11.84 LKK60 pKa = 11.04 HH61 pKa = 6.39 EE62 pKa = 4.79 DD63 pKa = 3.79 GDD65 pKa = 4.2 WIEE68 pKa = 4.24 FTFDD72 pKa = 4.87 QPKK75 pKa = 8.18 TWNLLDD81 pKa = 3.93 LQGGMHH87 pKa = 6.81 EE88 pKa = 4.31 PLITDD93 pKa = 3.75 EE94 pKa = 4.48 EE95 pKa = 5.05 VPAGDD100 pKa = 3.7 YY101 pKa = 10.69 TEE103 pKa = 4.47 LRR105 pKa = 11.84 LMVDD109 pKa = 3.14 TADD112 pKa = 3.67 SYY114 pKa = 11.83 VIEE117 pKa = 4.47 SSQPDD122 pKa = 3.75 VEE124 pKa = 4.43 KK125 pKa = 10.32 TLAVPSGEE133 pKa = 4.13 QSGLKK138 pKa = 10.35 LKK140 pKa = 11.14 GNFVVAADD148 pKa = 3.81 TSTDD152 pKa = 3.65 FTIDD156 pKa = 3.36 FNVAKK161 pKa = 10.52 SIVNPQGKK169 pKa = 9.05 SLADD173 pKa = 3.58 YY174 pKa = 10.53 LLKK177 pKa = 10.66 PSLRR181 pKa = 11.84 LTNTLEE187 pKa = 4.2 TGSITGEE194 pKa = 3.48 VDD196 pKa = 3.31 YY197 pKa = 8.81 PTINSTRR204 pKa = 11.84 SADD207 pKa = 3.59 ADD209 pKa = 3.94 LANCSDD215 pKa = 4.07 GYY217 pKa = 10.68 YY218 pKa = 10.88 GSIYY222 pKa = 9.85 VYY224 pKa = 10.1 QGAGVTPTDD233 pKa = 3.47 VNVNTEE239 pKa = 4.76 DD240 pKa = 4.09 GPLMTIPVKK249 pKa = 10.78 DD250 pKa = 4.09 DD251 pKa = 4.01 NNDD254 pKa = 2.89 GMYY257 pKa = 10.16 SYY259 pKa = 9.69 TAAYY263 pKa = 8.26 LTEE266 pKa = 4.31 GDD268 pKa = 3.68 YY269 pKa = 10.68 TLSYY273 pKa = 10.14 TCQLDD278 pKa = 4.23 DD279 pKa = 5.77 NEE281 pKa = 4.67 TTDD284 pKa = 3.7 DD285 pKa = 3.63 TVEE288 pKa = 4.12 FEE290 pKa = 4.3 GTQTVSVVAGEE301 pKa = 4.07 EE302 pKa = 4.15 TQADD306 pKa = 4.73 SIPLTPP312 pKa = 4.83
Molecular weight: 33.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.726
IPC2_protein 3.745
IPC_protein 3.77
Toseland 3.541
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|M7CRC4|M7CRC4_9ALTE Proton/sodium-glutamate symport protein OS=Marinobacter santoriniensis NKSG1 OX=1288826 GN=MSNKSG1_09968 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.34 RR12 pKa = 11.84 KK13 pKa = 9.1 RR14 pKa = 11.84 VHH16 pKa = 6.26 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.91 VLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.44 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3692
0
3692
1219654
16
2517
330.4
36.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.665 ± 0.042
0.937 ± 0.013
5.994 ± 0.031
6.471 ± 0.04
3.8 ± 0.027
7.853 ± 0.039
2.243 ± 0.023
5.228 ± 0.029
3.675 ± 0.033
10.67 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.548 ± 0.018
3.193 ± 0.024
4.744 ± 0.021
3.94 ± 0.031
6.723 ± 0.039
5.933 ± 0.03
5.196 ± 0.027
7.25 ± 0.036
1.377 ± 0.017
2.561 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here