beta proteobacterium AAP99

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; unclassified Betaproteobacteria

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N1LBV2|A0A0N1LBV2_9PROT Bifunctional protein GlmU OS=beta proteobacterium AAP99 OX=1523428 GN=glmU PE=3 SV=1
MM1 pKa = 7.69ALLITDD7 pKa = 3.2QCINCDD13 pKa = 3.35VCEE16 pKa = 4.18PEE18 pKa = 4.85CPNEE22 pKa = 4.32AIYY25 pKa = 9.97MGTEE29 pKa = 3.53IYY31 pKa = 10.33EE32 pKa = 4.11IEE34 pKa = 4.31PGKK37 pKa = 9.34CTEE40 pKa = 4.55CVGHH44 pKa = 6.76FNTPQCVEE52 pKa = 4.12VCPVEE57 pKa = 4.91CILVNPEE64 pKa = 3.58WQEE67 pKa = 4.23SKK69 pKa = 11.1DD70 pKa = 3.71DD71 pKa = 3.98LMAKK75 pKa = 9.64YY76 pKa = 9.97RR77 pKa = 11.84QLTGKK82 pKa = 9.95FDD84 pKa = 3.76AAIAAAAASAAAEE97 pKa = 4.18PDD99 pKa = 3.28QSNAKK104 pKa = 9.63AASPVV109 pKa = 3.19

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N1B8Y2|A0A0N1B8Y2_9PROT Bacteriochlorophyll/chlorophyll a synthase OS=beta proteobacterium AAP99 OX=1523428 GN=IP84_04810 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.58RR14 pKa = 11.84THH16 pKa = 6.08GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.8TKK25 pKa = 10.34GGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.6RR41 pKa = 11.84LAVAA45 pKa = 4.53

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3159

0

3159

1010340

37

3384

319.8

34.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.357 ± 0.075

0.871 ± 0.015

5.198 ± 0.03

5.261 ± 0.042

3.553 ± 0.028

8.228 ± 0.045

2.054 ± 0.027

4.467 ± 0.029

3.369 ± 0.043

10.435 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.417 ± 0.019

2.704 ± 0.035

5.256 ± 0.03

3.968 ± 0.03

7.054 ± 0.051

5.343 ± 0.036

5.311 ± 0.045

7.528 ± 0.033

1.484 ± 0.02

2.141 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski