Amino acid dipepetide frequency for beta proteobacterium AAP99

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.062AlaAla: 20.062 ± 0.25
1.233AlaCys: 1.233 ± 0.038
6.638AlaAsp: 6.638 ± 0.089
6.947AlaGlu: 6.947 ± 0.096
4.188AlaPhe: 4.188 ± 0.077
11.254AlaGly: 11.254 ± 0.135
2.518AlaHis: 2.518 ± 0.058
5.119AlaIle: 5.119 ± 0.072
4.129AlaLys: 4.129 ± 0.081
14.875AlaLeu: 14.875 ± 0.157
3.303AlaMet: 3.303 ± 0.048
3.153AlaAsn: 3.153 ± 0.07
6.919AlaPro: 6.919 ± 0.131
6.689AlaGln: 6.689 ± 0.096
9.583AlaArg: 9.583 ± 0.137
6.985AlaSer: 6.985 ± 0.1
6.278AlaThr: 6.278 ± 0.086
9.306AlaVal: 9.306 ± 0.102
1.934AlaTrp: 1.934 ± 0.047
2.453AlaTyr: 2.453 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
1.227CysAla: 1.227 ± 0.036
0.098CysCys: 0.098 ± 0.01
0.441CysAsp: 0.441 ± 0.019
0.566CysGlu: 0.566 ± 0.026
0.272CysPhe: 0.272 ± 0.014
0.944CysGly: 0.944 ± 0.031
0.223CysHis: 0.223 ± 0.015
0.373CysIle: 0.373 ± 0.02
0.241CysLys: 0.241 ± 0.018
0.7CysLeu: 0.7 ± 0.026
0.191CysMet: 0.191 ± 0.012
0.242CysAsn: 0.242 ± 0.014
0.472CysPro: 0.472 ± 0.022
0.217CysGln: 0.217 ± 0.015
0.483CysArg: 0.483 ± 0.025
0.479CysSer: 0.479 ± 0.022
0.526CysThr: 0.526 ± 0.044
0.717CysVal: 0.717 ± 0.031
0.111CysTrp: 0.111 ± 0.011
0.193CysTyr: 0.193 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.733AspAla: 7.733 ± 0.091
0.479AspCys: 0.479 ± 0.026
2.567AspAsp: 2.567 ± 0.052
3.189AspGlu: 3.189 ± 0.067
1.998AspPhe: 1.998 ± 0.052
4.328AspGly: 4.328 ± 0.072
0.977AspHis: 0.977 ± 0.031
2.202AspIle: 2.202 ± 0.047
1.719AspLys: 1.719 ± 0.052
5.287AspLeu: 5.287 ± 0.072
1.122AspMet: 1.122 ± 0.039
1.066AspAsn: 1.066 ± 0.033
3.039AspPro: 3.039 ± 0.059
1.635AspGln: 1.635 ± 0.038
3.476AspArg: 3.476 ± 0.066
2.313AspSer: 2.313 ± 0.044
2.646AspThr: 2.646 ± 0.05
3.886AspVal: 3.886 ± 0.062
0.982AspTrp: 0.982 ± 0.033
1.336AspTyr: 1.336 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
6.768GluAla: 6.768 ± 0.099
0.391GluCys: 0.391 ± 0.021
2.332GluAsp: 2.332 ± 0.055
2.234GluGlu: 2.234 ± 0.058
1.816GluPhe: 1.816 ± 0.041
3.706GluGly: 3.706 ± 0.062
1.359GluHis: 1.359 ± 0.038
2.725GluIle: 2.725 ± 0.052
1.775GluLys: 1.775 ± 0.051
5.888GluLeu: 5.888 ± 0.082
1.228GluMet: 1.228 ± 0.037
1.236GluAsn: 1.236 ± 0.035
2.616GluPro: 2.616 ± 0.053
2.651GluGln: 2.651 ± 0.059
5.06GluArg: 5.06 ± 0.092
2.577GluSer: 2.577 ± 0.055
2.479GluThr: 2.479 ± 0.047
4.058GluVal: 4.058 ± 0.069
0.663GluTrp: 0.663 ± 0.025
1.048GluTyr: 1.048 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
4.774PheAla: 4.774 ± 0.061
0.395PheCys: 0.395 ± 0.022
2.377PheAsp: 2.377 ± 0.047
2.134PheGlu: 2.134 ± 0.047
1.331PhePhe: 1.331 ± 0.04
3.403PheGly: 3.403 ± 0.065
0.694PheHis: 0.694 ± 0.032
1.587PheIle: 1.587 ± 0.045
1.327PheLys: 1.327 ± 0.036
2.841PheLeu: 2.841 ± 0.052
0.879PheMet: 0.879 ± 0.031
1.224PheAsn: 1.224 ± 0.038
1.401PhePro: 1.401 ± 0.032
0.937PheGln: 0.937 ± 0.029
1.919PheArg: 1.919 ± 0.045
2.155PheSer: 2.155 ± 0.049
2.026PheThr: 2.026 ± 0.044
2.753PheVal: 2.753 ± 0.051
0.536PheTrp: 0.536 ± 0.023
0.835PheTyr: 0.835 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
9.585GlyAla: 9.585 ± 0.133
0.871GlyCys: 0.871 ± 0.031
3.747GlyAsp: 3.747 ± 0.075
4.466GlyGlu: 4.466 ± 0.062
3.416GlyPhe: 3.416 ± 0.06
6.812GlyGly: 6.812 ± 0.133
1.678GlyHis: 1.678 ± 0.043
3.789GlyIle: 3.789 ± 0.066
3.497GlyLys: 3.497 ± 0.075
8.512GlyLeu: 8.512 ± 0.106
2.193GlyMet: 2.193 ± 0.05
2.199GlyAsn: 2.199 ± 0.061
3.033GlyPro: 3.033 ± 0.058
3.368GlyGln: 3.368 ± 0.067
5.455GlyArg: 5.455 ± 0.08
4.858GlySer: 4.858 ± 0.087
4.658GlyThr: 4.658 ± 0.084
6.616GlyVal: 6.616 ± 0.087
1.467GlyTrp: 1.467 ± 0.037
2.062GlyTyr: 2.062 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
2.729HisAla: 2.729 ± 0.056
0.244HisCys: 0.244 ± 0.016
1.049HisAsp: 1.049 ± 0.033
1.049HisGlu: 1.049 ± 0.034
0.878HisPhe: 0.878 ± 0.032
1.94HisGly: 1.94 ± 0.043
0.564HisHis: 0.564 ± 0.026
0.917HisIle: 0.917 ± 0.031
0.514HisLys: 0.514 ± 0.023
2.09HisLeu: 2.09 ± 0.047
0.458HisMet: 0.458 ± 0.024
0.435HisAsn: 0.435 ± 0.02
1.351HisPro: 1.351 ± 0.037
0.587HisGln: 0.587 ± 0.027
1.388HisArg: 1.388 ± 0.044
0.913HisSer: 0.913 ± 0.03
1.012HisThr: 1.012 ± 0.032
1.467HisVal: 1.467 ± 0.036
0.413HisTrp: 0.413 ± 0.021
0.539HisTyr: 0.539 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.05IleAla: 6.05 ± 0.079
0.396IleCys: 0.396 ± 0.019
3.232IleAsp: 3.232 ± 0.051
3.488IleGlu: 3.488 ± 0.06
1.238IlePhe: 1.238 ± 0.038
4.164IleGly: 4.164 ± 0.082
0.822IleHis: 0.822 ± 0.033
1.77IleIle: 1.77 ± 0.05
1.631IleLys: 1.631 ± 0.044
3.341IleLeu: 3.341 ± 0.059
0.854IleMet: 0.854 ± 0.028
1.547IleAsn: 1.547 ± 0.046
2.023IlePro: 2.023 ± 0.053
1.327IleGln: 1.327 ± 0.035
2.847IleArg: 2.847 ± 0.051
2.414IleSer: 2.414 ± 0.052
2.678IleThr: 2.678 ± 0.06
3.374IleVal: 3.374 ± 0.058
0.492IleTrp: 0.492 ± 0.025
0.986IleTyr: 0.986 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
4.111LysAla: 4.111 ± 0.086
0.165LysCys: 0.165 ± 0.012
1.892LysAsp: 1.892 ± 0.057
1.526LysGlu: 1.526 ± 0.054
0.996LysPhe: 0.996 ± 0.032
2.361LysGly: 2.361 ± 0.051
0.743LysHis: 0.743 ± 0.029
1.47LysIle: 1.47 ± 0.04
1.405LysLys: 1.405 ± 0.057
3.832LysLeu: 3.832 ± 0.068
0.844LysMet: 0.844 ± 0.034
0.893LysAsn: 0.893 ± 0.033
2.245LysPro: 2.245 ± 0.056
1.429LysGln: 1.429 ± 0.044
2.557LysArg: 2.557 ± 0.05
1.693LysSer: 1.693 ± 0.044
1.971LysThr: 1.971 ± 0.047
2.525LysVal: 2.525 ± 0.061
0.365LysTrp: 0.365 ± 0.019
0.665LysTyr: 0.665 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
15.248LeuAla: 15.248 ± 0.167
0.797LeuCys: 0.797 ± 0.029
5.653LeuAsp: 5.653 ± 0.083
4.83LeuGlu: 4.83 ± 0.076
3.27LeuPhe: 3.27 ± 0.066
8.2LeuGly: 8.2 ± 0.096
2.166LeuHis: 2.166 ± 0.043
4.676LeuIle: 4.676 ± 0.082
3.871LeuLys: 3.871 ± 0.077
10.718LeuLeu: 10.718 ± 0.151
2.458LeuMet: 2.458 ± 0.046
3.02LeuAsn: 3.02 ± 0.056
6.099LeuPro: 6.099 ± 0.09
3.752LeuGln: 3.752 ± 0.062
7.515LeuArg: 7.515 ± 0.108
6.087LeuSer: 6.087 ± 0.089
5.579LeuThr: 5.579 ± 0.079
7.154LeuVal: 7.154 ± 0.085
1.293LeuTrp: 1.293 ± 0.043
1.961LeuTyr: 1.961 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
2.918MetAla: 2.918 ± 0.053
0.143MetCys: 0.143 ± 0.013
1.2MetAsp: 1.2 ± 0.036
0.999MetGlu: 0.999 ± 0.032
0.69MetPhe: 0.69 ± 0.027
1.834MetGly: 1.834 ± 0.047
0.52MetHis: 0.52 ± 0.022
1.061MetIle: 1.061 ± 0.036
1.075MetLys: 1.075 ± 0.035
2.637MetLeu: 2.637 ± 0.053
0.571MetMet: 0.571 ± 0.023
0.919MetAsn: 0.919 ± 0.028
1.468MetPro: 1.468 ± 0.041
1.163MetGln: 1.163 ± 0.032
1.845MetArg: 1.845 ± 0.049
1.661MetSer: 1.661 ± 0.046
1.299MetThr: 1.299 ± 0.036
1.557MetVal: 1.557 ± 0.037
0.232MetTrp: 0.232 ± 0.018
0.381MetTyr: 0.381 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.577AsnAla: 3.577 ± 0.076
0.277AsnCys: 0.277 ± 0.02
1.287AsnAsp: 1.287 ± 0.036
1.287AsnGlu: 1.287 ± 0.039
1.024AsnPhe: 1.024 ± 0.035
2.169AsnGly: 2.169 ± 0.066
0.502AsnHis: 0.502 ± 0.021
1.234AsnIle: 1.234 ± 0.039
0.874AsnLys: 0.874 ± 0.029
2.753AsnLeu: 2.753 ± 0.062
0.585AsnMet: 0.585 ± 0.029
0.843AsnAsn: 0.843 ± 0.038
1.982AsnPro: 1.982 ± 0.053
0.951AsnGln: 0.951 ± 0.035
1.776AsnArg: 1.776 ± 0.046
1.245AsnSer: 1.245 ± 0.037
1.545AsnThr: 1.545 ± 0.046
2.023AsnVal: 2.023 ± 0.049
0.4AsnTrp: 0.4 ± 0.021
0.704AsnTyr: 0.704 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
7.866ProAla: 7.866 ± 0.132
0.348ProCys: 0.348 ± 0.018
3.106ProAsp: 3.106 ± 0.055
3.214ProGlu: 3.214 ± 0.062
1.743ProPhe: 1.743 ± 0.048
4.555ProGly: 4.555 ± 0.072
1.031ProHis: 1.031 ± 0.029
2.155ProIle: 2.155 ± 0.045
1.746ProLys: 1.746 ± 0.052
4.849ProLeu: 4.849 ± 0.074
1.313ProMet: 1.313 ± 0.037
1.45ProAsn: 1.45 ± 0.044
2.631ProPro: 2.631 ± 0.059
2.168ProGln: 2.168 ± 0.048
2.993ProArg: 2.993 ± 0.059
2.608ProSer: 2.608 ± 0.06
2.665ProThr: 2.665 ± 0.053
4.29ProVal: 4.29 ± 0.076
0.771ProTrp: 0.771 ± 0.028
1.062ProTyr: 1.062 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
5.721GlnAla: 5.721 ± 0.09
0.295GlnCys: 0.295 ± 0.017
1.645GlnAsp: 1.645 ± 0.042
1.333GlnGlu: 1.333 ± 0.039
1.422GlnPhe: 1.422 ± 0.042
3.003GlnGly: 3.003 ± 0.047
0.858GlnHis: 0.858 ± 0.031
1.961GlnIle: 1.961 ± 0.042
0.998GlnLys: 0.998 ± 0.033
4.206GlnLeu: 4.206 ± 0.062
1.043GlnMet: 1.043 ± 0.035
0.875GlnAsn: 0.875 ± 0.032
2.316GlnPro: 2.316 ± 0.043
1.878GlnGln: 1.878 ± 0.061
3.61GlnArg: 3.61 ± 0.064
1.947GlnSer: 1.947 ± 0.051
2.153GlnThr: 2.153 ± 0.055
2.956GlnVal: 2.956 ± 0.056
0.677GlnTrp: 0.677 ± 0.027
0.787GlnTyr: 0.787 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
8.525ArgAla: 8.525 ± 0.122
0.602ArgCys: 0.602 ± 0.023
3.627ArgAsp: 3.627 ± 0.064
4.385ArgGlu: 4.385 ± 0.079
3.061ArgPhe: 3.061 ± 0.061
4.962ArgGly: 4.962 ± 0.073
1.418ArgHis: 1.418 ± 0.04
3.752ArgIle: 3.752 ± 0.06
2.292ArgLys: 2.292 ± 0.048
7.645ArgLeu: 7.645 ± 0.102
2.037ArgMet: 2.037 ± 0.05
1.94ArgAsn: 1.94 ± 0.045
3.126ArgPro: 3.126 ± 0.06
2.506ArgGln: 2.506 ± 0.045
4.864ArgArg: 4.864 ± 0.09
3.691ArgSer: 3.691 ± 0.062
3.601ArgThr: 3.601 ± 0.057
5.478ArgVal: 5.478 ± 0.084
1.273ArgTrp: 1.273 ± 0.037
1.76ArgTyr: 1.76 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
6.866SerAla: 6.866 ± 0.101
0.432SerCys: 0.432 ± 0.029
2.596SerAsp: 2.596 ± 0.058
2.671SerGlu: 2.671 ± 0.043
2.135SerPhe: 2.135 ± 0.046
5.207SerGly: 5.207 ± 0.085
1.04SerHis: 1.04 ± 0.034
2.516SerIle: 2.516 ± 0.059
1.65SerLys: 1.65 ± 0.046
5.409SerLeu: 5.409 ± 0.08
1.321SerMet: 1.321 ± 0.032
1.441SerAsn: 1.441 ± 0.041
2.711SerPro: 2.711 ± 0.053
1.778SerGln: 1.778 ± 0.048
3.587SerArg: 3.587 ± 0.061
2.95SerSer: 2.95 ± 0.068
3.124SerThr: 3.124 ± 0.068
4.061SerVal: 4.061 ± 0.07
0.781SerTrp: 0.781 ± 0.028
1.157SerTyr: 1.157 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
6.454ThrAla: 6.454 ± 0.096
0.449ThrCys: 0.449 ± 0.049
2.829ThrAsp: 2.829 ± 0.054
2.7ThrGlu: 2.7 ± 0.056
1.675ThrPhe: 1.675 ± 0.043
4.805ThrGly: 4.805 ± 0.09
1.167ThrHis: 1.167 ± 0.03
2.16ThrIle: 2.16 ± 0.057
1.332ThrLys: 1.332 ± 0.035
6.248ThrLeu: 6.248 ± 0.085
1.014ThrMet: 1.014 ± 0.036
1.332ThrAsn: 1.332 ± 0.045
3.572ThrPro: 3.572 ± 0.065
2.267ThrGln: 2.267 ± 0.054
3.521ThrArg: 3.521 ± 0.066
2.576ThrSer: 2.576 ± 0.06
2.809ThrThr: 2.809 ± 0.064
4.301ThrVal: 4.301 ± 0.075
0.759ThrTrp: 0.759 ± 0.027
1.144ThrTyr: 1.144 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
9.247ValAla: 9.247 ± 0.123
0.685ValCys: 0.685 ± 0.027
4.032ValAsp: 4.032 ± 0.072
3.896ValGlu: 3.896 ± 0.068
2.853ValPhe: 2.853 ± 0.05
5.679ValGly: 5.679 ± 0.073
1.524ValHis: 1.524 ± 0.037
3.707ValIle: 3.707 ± 0.06
2.491ValLys: 2.491 ± 0.061
8.249ValLeu: 8.249 ± 0.097
1.863ValMet: 1.863 ± 0.045
2.253ValAsn: 2.253 ± 0.05
3.868ValPro: 3.868 ± 0.063
2.918ValGln: 2.918 ± 0.053
5.214ValArg: 5.214 ± 0.077
4.336ValSer: 4.336 ± 0.076
4.055ValThr: 4.055 ± 0.088
5.901ValVal: 5.901 ± 0.096
1.034ValTrp: 1.034 ± 0.032
1.476ValTyr: 1.476 ± 0.039
0.0ValXaa: 0.0 ± 0.0
Trp
1.545TrpAla: 1.545 ± 0.043
0.151TrpCys: 0.151 ± 0.013
0.63TrpAsp: 0.63 ± 0.025
0.535TrpGlu: 0.535 ± 0.023
0.599TrpPhe: 0.599 ± 0.028
0.999TrpGly: 0.999 ± 0.035
0.339TrpHis: 0.339 ± 0.018
0.71TrpIle: 0.71 ± 0.028
0.467TrpLys: 0.467 ± 0.024
2.082TrpLeu: 2.082 ± 0.051
0.443TrpMet: 0.443 ± 0.022
0.415TrpAsn: 0.415 ± 0.021
0.696TrpPro: 0.696 ± 0.031
0.745TrpGln: 0.745 ± 0.027
1.217TrpArg: 1.217 ± 0.041
0.747TrpSer: 0.747 ± 0.025
0.773TrpThr: 0.773 ± 0.028
1.14TrpVal: 1.14 ± 0.039
0.282TrpTrp: 0.282 ± 0.019
0.322TrpTyr: 0.322 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.561TyrAla: 2.561 ± 0.052
0.263TyrCys: 0.263 ± 0.016
1.1TyrAsp: 1.1 ± 0.035
1.161TyrGlu: 1.161 ± 0.034
0.922TyrPhe: 0.922 ± 0.031
1.956TyrGly: 1.956 ± 0.05
0.394TyrHis: 0.394 ± 0.02
0.793TyrIle: 0.793 ± 0.029
0.654TyrLys: 0.654 ± 0.028
2.183TyrLeu: 2.183 ± 0.049
0.446TyrMet: 0.446 ± 0.019
0.554TyrAsn: 0.554 ± 0.023
0.998TyrPro: 0.998 ± 0.031
0.718TyrGln: 0.718 ± 0.027
1.637TyrArg: 1.637 ± 0.041
1.198TyrSer: 1.198 ± 0.039
1.238TyrThr: 1.238 ± 0.041
1.719TyrVal: 1.719 ± 0.043
0.375TyrTrp: 0.375 ± 0.022
0.541TyrTyr: 0.541 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3159 proteins (1010340 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski