Streptococcus phage phiZJ20091101-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9I5B1|A0A1X9I5B1_9CAUD Uncharacterized protein OS=Streptococcus phage phiZJ20091101-1 OX=1860195 PE=4 SV=1
MM1 pKa = 8.16SEE3 pKa = 4.2IKK5 pKa = 10.77NNPHH9 pKa = 6.22SLATKK14 pKa = 9.82AGLVEE19 pKa = 4.87EE20 pKa = 4.25IRR22 pKa = 11.84GALSLPYY29 pKa = 9.87EE30 pKa = 4.45YY31 pKa = 10.92YY32 pKa = 10.8LKK34 pKa = 10.48QEE36 pKa = 4.25SEE38 pKa = 4.0IKK40 pKa = 10.58ALAKK44 pKa = 10.31IHH46 pKa = 6.66DD47 pKa = 4.22CEE49 pKa = 5.97IILVDD54 pKa = 4.28AEE56 pKa = 4.41YY57 pKa = 10.52TLTYY61 pKa = 10.35PNGEE65 pKa = 4.3EE66 pKa = 3.95VSKK69 pKa = 10.75IIPKK73 pKa = 10.0EE74 pKa = 4.06SNLFDD79 pKa = 5.09DD80 pKa = 5.07LMEE83 pKa = 4.86AFKK86 pKa = 10.88QLL88 pKa = 3.34

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9I5H2|A0A1X9I5H2_9CAUD Putative transcriptional regulator of pyridoxine OS=Streptococcus phage phiZJ20091101-1 OX=1860195 PE=3 SV=1
MM1 pKa = 7.56KK2 pKa = 10.29KK3 pKa = 10.0CLCQAAFAIKK13 pKa = 9.43KK14 pKa = 9.81QKK16 pKa = 10.44GSPLAEE22 pKa = 3.81RR23 pKa = 11.84YY24 pKa = 9.39YY25 pKa = 10.44QIQSRR30 pKa = 11.84RR31 pKa = 11.84GSQKK35 pKa = 9.27ATIALAHH42 pKa = 6.08QLLKK46 pKa = 10.22IAYY49 pKa = 8.34ILLKK53 pKa = 10.44EE54 pKa = 4.13QITYY58 pKa = 10.54PEE60 pKa = 4.02FLAQKK65 pKa = 8.92KK66 pKa = 4.49TTRR69 pKa = 11.84DD70 pKa = 3.26EE71 pKa = 4.2LVAA74 pKa = 5.25

Molecular weight: 8.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

9527

46

822

207.1

23.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.599 ± 0.549

0.756 ± 0.101

6.445 ± 0.269

7.022 ± 0.244

3.947 ± 0.238

5.952 ± 0.313

1.889 ± 0.223

6.697 ± 0.257

7.872 ± 0.424

9.342 ± 0.495

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.477 ± 0.142

4.839 ± 0.242

2.95 ± 0.222

4.608 ± 0.349

4.545 ± 0.256

6.088 ± 0.266

5.658 ± 0.324

6.508 ± 0.312

0.913 ± 0.115

3.894 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski