Avrilella dinanensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Avrilella

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2179 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M9R2S8|A0A2M9R2S8_9FLAO 3-deoxy-D-manno-octulosonic acid transferase OS=Avrilella dinanensis OX=2008672 GN=CDL10_11310 PE=3 SV=1
MM1 pKa = 7.23VFSCSEE7 pKa = 4.29DD8 pKa = 3.37YY9 pKa = 10.67TGNSDD14 pKa = 3.53GSSDD18 pKa = 3.31NSEE21 pKa = 4.08YY22 pKa = 11.27NSLKK26 pKa = 10.63NVNTPVTDD34 pKa = 3.61YY35 pKa = 11.16AFEE38 pKa = 4.57DD39 pKa = 3.45MRR41 pKa = 11.84IDD43 pKa = 4.35IGIEE47 pKa = 3.66LLKK50 pKa = 10.24VPKK53 pKa = 10.15RR54 pKa = 11.84IRR56 pKa = 11.84AMTNTSDD63 pKa = 3.93LTNKK67 pKa = 10.43ALDD70 pKa = 3.54CSFGFGFCITISIDD84 pKa = 2.56WDD86 pKa = 4.37AIQAPHH92 pKa = 6.94LGGVDD97 pKa = 4.59PITTGEE103 pKa = 4.0NQVIAVYY110 pKa = 10.3KK111 pKa = 9.71PDD113 pKa = 4.63EE114 pKa = 4.07ITEE117 pKa = 4.28TVTFYY122 pKa = 11.03FPNDD126 pKa = 3.58LTSLDD131 pKa = 3.7QFSEE135 pKa = 3.93EE136 pKa = 4.78DD137 pKa = 3.24IQEE140 pKa = 4.03FTVFEE145 pKa = 4.51DD146 pKa = 3.88LEE148 pKa = 4.27IAEE151 pKa = 4.5GMVLKK156 pKa = 10.76PGDD159 pKa = 3.74YY160 pKa = 10.07PLQYY164 pKa = 11.04DD165 pKa = 3.75SLGNLAYY172 pKa = 10.63VVDD175 pKa = 4.87MYY177 pKa = 11.86

Molecular weight: 19.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M9R7B6|A0A2M9R7B6_9FLAO Enoyl-CoA hydratase OS=Avrilella dinanensis OX=2008672 GN=CDL10_09080 PE=4 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGFDD22 pKa = 5.49AITTDD27 pKa = 3.1KK28 pKa = 11.09PEE30 pKa = 4.48RR31 pKa = 11.84SLLAPKK37 pKa = 10.11KK38 pKa = 10.57SSGGRR43 pKa = 11.84NSQGKK48 pKa = 6.14MTVRR52 pKa = 11.84YY53 pKa = 9.78KK54 pKa = 11.13GGGHH58 pKa = 6.43KK59 pKa = 9.83KK60 pKa = 9.97RR61 pKa = 11.84YY62 pKa = 9.36RR63 pKa = 11.84LIDD66 pKa = 3.69FKK68 pKa = 11.1RR69 pKa = 11.84NKK71 pKa = 9.85FGVPATVKK79 pKa = 10.59SIEE82 pKa = 4.0YY83 pKa = 10.15DD84 pKa = 3.43PNRR87 pKa = 11.84TAFIALLAYY96 pKa = 10.3ADD98 pKa = 3.73GEE100 pKa = 4.14KK101 pKa = 10.18RR102 pKa = 11.84YY103 pKa = 10.15IIAPSGLQVGQTVVSGPDD121 pKa = 3.12AAPEE125 pKa = 4.12VGNTMPLANIPLGTVISCIEE145 pKa = 3.87LRR147 pKa = 11.84PGEE150 pKa = 4.24GALIARR156 pKa = 11.84SAGTFAQLVARR167 pKa = 11.84DD168 pKa = 3.8GKK170 pKa = 10.13YY171 pKa = 9.11ATVKK175 pKa = 9.8MPSGEE180 pKa = 3.81IRR182 pKa = 11.84LVLLTCVATIGSVSNHH198 pKa = 5.29DD199 pKa = 3.62HH200 pKa = 5.97QLLVSGKK207 pKa = 9.49AGRR210 pKa = 11.84SRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPIDD229 pKa = 3.81HH230 pKa = 7.13PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.7PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.25GYY255 pKa = 6.97RR256 pKa = 11.84TRR258 pKa = 11.84SRR260 pKa = 11.84VKK262 pKa = 10.04YY263 pKa = 7.59SNKK266 pKa = 10.16YY267 pKa = 8.25IVEE270 pKa = 4.08RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2179

0

2179

700026

26

3715

321.3

36.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.247 ± 0.054

0.727 ± 0.018

5.551 ± 0.038

7.071 ± 0.057

5.188 ± 0.038

6.227 ± 0.046

1.817 ± 0.023

8.03 ± 0.054

7.444 ± 0.071

9.077 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.342 ± 0.024

6.031 ± 0.049

3.314 ± 0.03

3.939 ± 0.038

3.573 ± 0.037

6.161 ± 0.043

5.656 ± 0.055

6.345 ± 0.04

1.002 ± 0.021

4.255 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski