Capsicum annuum amalgavirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2V0A0C7|A0A2V0A0C7_9VIRU ORF1+2p OS=Capsicum annuum amalgavirus 1 OX=2069320 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.58SGTSGPRR8 pKa = 11.84TPGSSGVKK16 pKa = 8.81TVPLTARR23 pKa = 11.84EE24 pKa = 4.02KK25 pKa = 9.13EE26 pKa = 3.64IAMIEE31 pKa = 4.18EE32 pKa = 3.92IDD34 pKa = 4.04EE35 pKa = 4.34IGVTFIEE42 pKa = 4.81LGVSAAFFDD51 pKa = 4.05SRR53 pKa = 11.84VYY55 pKa = 10.01TKK57 pKa = 11.11NLLLPAQYY65 pKa = 10.86LRR67 pKa = 11.84LLRR70 pKa = 11.84QFKK73 pKa = 10.69GKK75 pKa = 10.03DD76 pKa = 3.02AGEE79 pKa = 4.15VEE81 pKa = 5.45LIFSAAIAKK90 pKa = 9.32KK91 pKa = 7.93QVRR94 pKa = 11.84ASRR97 pKa = 11.84GIGWNKK103 pKa = 9.39WIQFLNWAKK112 pKa = 10.82SPAGHH117 pKa = 7.03KK118 pKa = 10.27AIQEE122 pKa = 3.9VMNIEE127 pKa = 3.92KK128 pKa = 10.66LEE130 pKa = 3.93RR131 pKa = 11.84RR132 pKa = 11.84GGGDD136 pKa = 3.28FTVDD140 pKa = 3.08EE141 pKa = 4.57VAALNLFDD149 pKa = 3.95VQRR152 pKa = 11.84NDD154 pKa = 3.44WIGHH158 pKa = 4.43QKK160 pKa = 8.49EE161 pKa = 3.74ARR163 pKa = 11.84AIAEE167 pKa = 4.07HH168 pKa = 6.99EE169 pKa = 4.01IAEE172 pKa = 4.28LQRR175 pKa = 11.84KK176 pKa = 8.79INLRR180 pKa = 11.84RR181 pKa = 11.84DD182 pKa = 3.39KK183 pKa = 10.99LDD185 pKa = 3.37EE186 pKa = 4.49DD187 pKa = 3.69LRR189 pKa = 11.84EE190 pKa = 4.07IADD193 pKa = 3.2QHH195 pKa = 6.63RR196 pKa = 11.84PVSGYY201 pKa = 10.67VPLTDD206 pKa = 4.4AEE208 pKa = 4.51LNLRR212 pKa = 11.84CWNFFRR218 pKa = 11.84QAHH221 pKa = 5.36EE222 pKa = 4.28GVRR225 pKa = 11.84GVGVHH230 pKa = 5.48PTMSQMKK237 pKa = 9.2EE238 pKa = 3.76AFDD241 pKa = 3.96TYY243 pKa = 10.75SVHH246 pKa = 4.62VAKK249 pKa = 10.51RR250 pKa = 11.84ARR252 pKa = 11.84VEE254 pKa = 3.86YY255 pKa = 9.09MRR257 pKa = 11.84HH258 pKa = 5.65GDD260 pKa = 4.02HH261 pKa = 7.27VPLLQEE267 pKa = 4.64YY268 pKa = 9.06INQKK272 pKa = 9.15IAHH275 pKa = 6.71FDD277 pKa = 3.25AVGDD281 pKa = 3.81KK282 pKa = 10.62RR283 pKa = 11.84QVRR286 pKa = 11.84HH287 pKa = 6.49LSLQLAGMGYY297 pKa = 10.16EE298 pKa = 4.29VDD300 pKa = 3.77GKK302 pKa = 9.94MGRR305 pKa = 11.84ADD307 pKa = 3.44EE308 pKa = 4.85GEE310 pKa = 4.07ADD312 pKa = 3.72EE313 pKa = 4.57GHH315 pKa = 7.1PGGDD319 pKa = 3.38VEE321 pKa = 5.81PEE323 pKa = 4.24GEE325 pKa = 4.54PDD327 pKa = 3.38PLLATFGTNPDD338 pKa = 3.38EE339 pKa = 4.21QVAGEE344 pKa = 4.49EE345 pKa = 4.7GDD347 pKa = 4.75LIPPGTSTQQDD358 pKa = 3.46VGVQGRR364 pKa = 11.84VTGPVTRR371 pKa = 11.84QRR373 pKa = 11.84ARR375 pKa = 3.12

Molecular weight:
41.75 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2V0A0C7|A0A2V0A0C7_9VIRU ORF1+2p OS=Capsicum annuum amalgavirus 1 OX=2069320 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.58SGTSGPRR8 pKa = 11.84TPGSSGVKK16 pKa = 8.81TVPLTARR23 pKa = 11.84EE24 pKa = 4.02KK25 pKa = 9.13EE26 pKa = 3.64IAMIEE31 pKa = 4.18EE32 pKa = 3.92IDD34 pKa = 4.04EE35 pKa = 4.34IGVTFIEE42 pKa = 4.81LGVSAAFFDD51 pKa = 4.05SRR53 pKa = 11.84VYY55 pKa = 10.01TKK57 pKa = 11.11NLLLPAQYY65 pKa = 10.86LRR67 pKa = 11.84LLRR70 pKa = 11.84QFKK73 pKa = 10.69GKK75 pKa = 10.03DD76 pKa = 3.02AGEE79 pKa = 4.15VEE81 pKa = 5.45LIFSAAIAKK90 pKa = 9.32KK91 pKa = 7.93QVRR94 pKa = 11.84ASRR97 pKa = 11.84GIGWNKK103 pKa = 9.39WIQFLNWAKK112 pKa = 10.82SPAGHH117 pKa = 7.03KK118 pKa = 10.27AIQEE122 pKa = 3.9VMNIEE127 pKa = 3.92KK128 pKa = 10.66LEE130 pKa = 3.93RR131 pKa = 11.84RR132 pKa = 11.84GGGDD136 pKa = 3.28FTVDD140 pKa = 3.08EE141 pKa = 4.57VAALNLFDD149 pKa = 3.95VQRR152 pKa = 11.84NDD154 pKa = 3.44WIGHH158 pKa = 4.43QKK160 pKa = 8.49EE161 pKa = 3.74ARR163 pKa = 11.84AIAEE167 pKa = 4.07HH168 pKa = 6.99EE169 pKa = 4.01IAEE172 pKa = 4.28LQRR175 pKa = 11.84KK176 pKa = 8.79INLRR180 pKa = 11.84RR181 pKa = 11.84DD182 pKa = 3.39KK183 pKa = 10.99LDD185 pKa = 3.37EE186 pKa = 4.49DD187 pKa = 3.69LRR189 pKa = 11.84EE190 pKa = 4.07IADD193 pKa = 3.2QHH195 pKa = 6.63RR196 pKa = 11.84PVSGYY201 pKa = 10.67VPLTDD206 pKa = 4.4AEE208 pKa = 4.51LNLRR212 pKa = 11.84CWNFFRR218 pKa = 11.84QAHH221 pKa = 5.36EE222 pKa = 4.28GVRR225 pKa = 11.84GVGVHH230 pKa = 5.48PTMSQMKK237 pKa = 9.2EE238 pKa = 3.76AFDD241 pKa = 3.96TYY243 pKa = 10.75SVHH246 pKa = 4.62VAKK249 pKa = 10.51RR250 pKa = 11.84ARR252 pKa = 11.84VEE254 pKa = 3.86YY255 pKa = 9.09MRR257 pKa = 11.84HH258 pKa = 5.65GDD260 pKa = 4.02HH261 pKa = 7.27VPLLQEE267 pKa = 4.64YY268 pKa = 9.06INQKK272 pKa = 9.15IAHH275 pKa = 6.71FDD277 pKa = 3.25AVGDD281 pKa = 3.81KK282 pKa = 10.58RR283 pKa = 11.84QVRR286 pKa = 11.84HH287 pKa = 7.0LIFNWQEE294 pKa = 3.61WVMKK298 pKa = 10.1WMVKK302 pKa = 9.05WDD304 pKa = 3.26VQTRR308 pKa = 11.84VKK310 pKa = 10.66LMRR313 pKa = 11.84DD314 pKa = 3.12IPVGTLNPRR323 pKa = 11.84GNQTLCSRR331 pKa = 11.84LSEE334 pKa = 4.33QIPMNRR340 pKa = 11.84LLEE343 pKa = 4.11KK344 pKa = 10.37RR345 pKa = 11.84VISYY349 pKa = 10.51RR350 pKa = 11.84PAPQLSKK357 pKa = 10.43MLEE360 pKa = 4.19CKK362 pKa = 10.59AEE364 pKa = 4.04SLALLRR370 pKa = 11.84ANGRR374 pKa = 11.84VEE376 pKa = 4.25IIIMPGGCDD385 pKa = 4.09LRR387 pKa = 11.84AIPCARR393 pKa = 11.84SRR395 pKa = 11.84WEE397 pKa = 3.43KK398 pKa = 10.62GIRR401 pKa = 11.84KK402 pKa = 9.41IIGGGEE408 pKa = 3.81MLNWKK413 pKa = 9.47VAGNMYY419 pKa = 10.31RR420 pKa = 11.84GGGCYY425 pKa = 9.92SDD427 pKa = 5.08AIKK430 pKa = 10.69LLSSASTIPPGRR442 pKa = 11.84LLHH445 pKa = 6.0QCFSLPGARR454 pKa = 11.84AALGLPSNLSVPVGKK469 pKa = 10.62GSCRR473 pKa = 11.84VKK475 pKa = 10.79NYY477 pKa = 10.18NDD479 pKa = 3.49EE480 pKa = 4.26ATTGPFLYY488 pKa = 10.81SFGIKK493 pKa = 9.6KK494 pKa = 10.39KK495 pKa = 10.57YY496 pKa = 10.6GLDD499 pKa = 3.54NEE501 pKa = 4.47LQEE504 pKa = 4.31IMEE507 pKa = 4.37NAYY510 pKa = 10.31HH511 pKa = 6.97HH512 pKa = 6.39FAEE515 pKa = 5.25CKK517 pKa = 8.57TSSSALPFFTARR529 pKa = 11.84VGFRR533 pKa = 11.84SKK535 pKa = 10.69LLPMGEE541 pKa = 4.35ALRR544 pKa = 11.84KK545 pKa = 9.16FADD548 pKa = 3.68NQPMGRR554 pKa = 11.84CVMMMDD560 pKa = 4.39ALEE563 pKa = 4.24QFASSPLYY571 pKa = 10.57NVLSKK576 pKa = 10.17YY577 pKa = 9.55TSDD580 pKa = 3.38RR581 pKa = 11.84SKK583 pKa = 11.09GATSFRR589 pKa = 11.84NSVVRR594 pKa = 11.84ASSDD598 pKa = 2.51WMYY601 pKa = 11.47LWDD604 pKa = 3.95EE605 pKa = 4.2VKK607 pKa = 10.4EE608 pKa = 4.02ASVCVEE614 pKa = 4.19LDD616 pKa = 2.69WSKK619 pKa = 10.97FDD621 pKa = 4.67RR622 pKa = 11.84EE623 pKa = 4.23RR624 pKa = 11.84PSADD628 pKa = 3.71LLFMIDD634 pKa = 3.7VIISCFEE641 pKa = 3.66PSNRR645 pKa = 11.84YY646 pKa = 6.72EE647 pKa = 3.88VRR649 pKa = 11.84MLEE652 pKa = 4.14AYY654 pKa = 8.38GICMRR659 pKa = 11.84RR660 pKa = 11.84ALVEE664 pKa = 4.01RR665 pKa = 11.84VLITDD670 pKa = 3.72DD671 pKa = 3.8GGVFEE676 pKa = 5.08IEE678 pKa = 4.09GMVPSGSLWTGWLDD692 pKa = 3.31TALNILYY699 pKa = 10.47LNAALRR705 pKa = 11.84HH706 pKa = 5.69LDD708 pKa = 3.43IAPSSASPKK717 pKa = 10.37CAGDD721 pKa = 3.87DD722 pKa = 3.72NLTLFYY728 pKa = 10.22RR729 pKa = 11.84DD730 pKa = 3.92PGDD733 pKa = 3.63EE734 pKa = 3.89VLLRR738 pKa = 11.84LKK740 pKa = 10.72VVLNEE745 pKa = 3.73WFRR748 pKa = 11.84AGIKK752 pKa = 10.67DD753 pKa = 3.54EE754 pKa = 4.96DD755 pKa = 4.21FVITRR760 pKa = 11.84PPYY763 pKa = 9.99HH764 pKa = 6.36VRR766 pKa = 11.84TYY768 pKa = 10.78QAVFPSGLDD777 pKa = 3.46LSKK780 pKa = 10.26GTSKK784 pKa = 10.42IIHH787 pKa = 5.75KK788 pKa = 10.25AYY790 pKa = 8.8WRR792 pKa = 11.84EE793 pKa = 3.77FEE795 pKa = 4.72DD796 pKa = 3.99EE797 pKa = 4.16VRR799 pKa = 11.84VDD801 pKa = 3.35MEE803 pKa = 4.48RR804 pKa = 11.84GLSHH808 pKa = 6.59RR809 pKa = 11.84WEE811 pKa = 4.18YY812 pKa = 10.85RR813 pKa = 11.84FKK815 pKa = 10.97GCPKK819 pKa = 10.18FLSCYY824 pKa = 7.7WLEE827 pKa = 4.37DD828 pKa = 3.27GRR830 pKa = 11.84PIRR833 pKa = 11.84PSSEE837 pKa = 3.84NLEE840 pKa = 3.88KK841 pKa = 11.0LLFPEE846 pKa = 5.71GVHH849 pKa = 6.99SSIDD853 pKa = 3.58DD854 pKa = 4.04YY855 pKa = 11.26IASVLSMVVDD865 pKa = 4.17NPFNDD870 pKa = 3.55HH871 pKa = 6.95NINHH875 pKa = 6.88LKK877 pKa = 10.03HH878 pKa = 6.7RR879 pKa = 11.84YY880 pKa = 8.73LIAMQIKK887 pKa = 9.52RR888 pKa = 11.84LSAAGGRR895 pKa = 11.84CGDD898 pKa = 3.29IMDD901 pKa = 5.3LARR904 pKa = 11.84IRR906 pKa = 11.84PRR908 pKa = 11.84HH909 pKa = 5.77DD910 pKa = 3.26EE911 pKa = 4.2EE912 pKa = 5.2VPVPQIAVWRR922 pKa = 11.84RR923 pKa = 11.84VKK925 pKa = 10.54EE926 pKa = 4.0YY927 pKa = 10.49IDD929 pKa = 3.93LDD931 pKa = 3.74EE932 pKa = 4.78YY933 pKa = 11.55GPTKK937 pKa = 10.53HH938 pKa = 6.83YY939 pKa = 10.41IDD941 pKa = 4.53EE942 pKa = 4.73FNSFVTGVTSLYY954 pKa = 11.07ARR956 pKa = 11.84ATTGGLDD963 pKa = 3.17AYY965 pKa = 10.18KK966 pKa = 11.12VMDD969 pKa = 5.29LIKK972 pKa = 10.3GNSPIGRR979 pKa = 11.84GQWGNDD985 pKa = 2.59VMAWIRR991 pKa = 11.84FVRR994 pKa = 11.84DD995 pKa = 3.55HH996 pKa = 6.95PATRR1000 pKa = 11.84YY1001 pKa = 9.62LKK1003 pKa = 8.66EE1004 pKa = 3.66TRR1006 pKa = 11.84RR1007 pKa = 11.84YY1008 pKa = 7.92KK1009 pKa = 11.11DD1010 pKa = 3.28EE1011 pKa = 4.99MIHH1014 pKa = 7.31IDD1016 pKa = 3.39QQTPLRR1022 pKa = 11.84PEE1024 pKa = 3.75VTGALAILSNALFNYY1039 pKa = 9.52VYY1041 pKa = 10.22EE1042 pKa = 4.51DD1043 pKa = 3.2SRR1045 pKa = 11.84EE1046 pKa = 3.93FSLSIANRR1054 pKa = 11.84IRR1056 pKa = 11.84TKK1058 pKa = 10.7RR1059 pKa = 11.84SVNN1062 pKa = 3.33

Molecular weight:
121.05 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1437

375

1062

718.5

81.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.724 ± 0.541

1.253 ± 0.496

5.985 ± 0.209

7.168 ± 0.82

3.758 ± 0.146

7.655 ± 1.112

2.644 ± 0.279

5.776 ± 0.356

5.358 ± 0.147

8.699 ± 0.619

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.784 ± 0.327

3.688 ± 0.379

4.523 ± 0.005

3.41 ± 0.967

8.142 ± 0.205

5.846 ± 1.062

3.688 ± 0.291

6.959 ± 0.389

1.949 ± 0.309

2.992 ± 0.566

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski