Capsicum annuum amalgavirus 1
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V0A0C7|A0A2V0A0C7_9VIRU ORF1+2p OS=Capsicum annuum amalgavirus 1 OX=2069320 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.58 SGTSGPRR8 pKa = 11.84 TPGSSGVKK16 pKa = 8.81 TVPLTARR23 pKa = 11.84 EE24 pKa = 4.02 KK25 pKa = 9.13 EE26 pKa = 3.64 IAMIEE31 pKa = 4.18 EE32 pKa = 3.92 IDD34 pKa = 4.04 EE35 pKa = 4.34 IGVTFIEE42 pKa = 4.81 LGVSAAFFDD51 pKa = 4.05 SRR53 pKa = 11.84 VYY55 pKa = 10.01 TKK57 pKa = 11.11 NLLLPAQYY65 pKa = 10.86 LRR67 pKa = 11.84 LLRR70 pKa = 11.84 QFKK73 pKa = 10.69 GKK75 pKa = 10.03 DD76 pKa = 3.02 AGEE79 pKa = 4.15 VEE81 pKa = 5.45 LIFSAAIAKK90 pKa = 9.32 KK91 pKa = 7.93 QVRR94 pKa = 11.84 ASRR97 pKa = 11.84 GIGWNKK103 pKa = 9.39 WIQFLNWAKK112 pKa = 10.82 SPAGHH117 pKa = 7.03 KK118 pKa = 10.27 AIQEE122 pKa = 3.9 VMNIEE127 pKa = 3.92 KK128 pKa = 10.66 LEE130 pKa = 3.93 RR131 pKa = 11.84 RR132 pKa = 11.84 GGGDD136 pKa = 3.28 FTVDD140 pKa = 3.08 EE141 pKa = 4.57 VAALNLFDD149 pKa = 3.95 VQRR152 pKa = 11.84 NDD154 pKa = 3.44 WIGHH158 pKa = 4.43 QKK160 pKa = 8.49 EE161 pKa = 3.74 ARR163 pKa = 11.84 AIAEE167 pKa = 4.07 HH168 pKa = 6.99 EE169 pKa = 4.01 IAEE172 pKa = 4.28 LQRR175 pKa = 11.84 KK176 pKa = 8.79 INLRR180 pKa = 11.84 RR181 pKa = 11.84 DD182 pKa = 3.39 KK183 pKa = 10.99 LDD185 pKa = 3.37 EE186 pKa = 4.49 DD187 pKa = 3.69 LRR189 pKa = 11.84 EE190 pKa = 4.07 IADD193 pKa = 3.2 QHH195 pKa = 6.63 RR196 pKa = 11.84 PVSGYY201 pKa = 10.67 VPLTDD206 pKa = 4.4 AEE208 pKa = 4.51 LNLRR212 pKa = 11.84 CWNFFRR218 pKa = 11.84 QAHH221 pKa = 5.36 EE222 pKa = 4.28 GVRR225 pKa = 11.84 GVGVHH230 pKa = 5.48 PTMSQMKK237 pKa = 9.2 EE238 pKa = 3.76 AFDD241 pKa = 3.96 TYY243 pKa = 10.75 SVHH246 pKa = 4.62 VAKK249 pKa = 10.51 RR250 pKa = 11.84 ARR252 pKa = 11.84 VEE254 pKa = 3.86 YY255 pKa = 9.09 MRR257 pKa = 11.84 HH258 pKa = 5.65 GDD260 pKa = 4.02 HH261 pKa = 7.27 VPLLQEE267 pKa = 4.64 YY268 pKa = 9.06 INQKK272 pKa = 9.15 IAHH275 pKa = 6.71 FDD277 pKa = 3.25 AVGDD281 pKa = 3.81 KK282 pKa = 10.62 RR283 pKa = 11.84 QVRR286 pKa = 11.84 HH287 pKa = 6.49 LSLQLAGMGYY297 pKa = 10.16 EE298 pKa = 4.29 VDD300 pKa = 3.77 GKK302 pKa = 9.94 MGRR305 pKa = 11.84 ADD307 pKa = 3.44 EE308 pKa = 4.85 GEE310 pKa = 4.07 ADD312 pKa = 3.72 EE313 pKa = 4.57 GHH315 pKa = 7.1 PGGDD319 pKa = 3.38 VEE321 pKa = 5.81 PEE323 pKa = 4.24 GEE325 pKa = 4.54 PDD327 pKa = 3.38 PLLATFGTNPDD338 pKa = 3.38 EE339 pKa = 4.21 QVAGEE344 pKa = 4.49 EE345 pKa = 4.7 GDD347 pKa = 4.75 LIPPGTSTQQDD358 pKa = 3.46 VGVQGRR364 pKa = 11.84 VTGPVTRR371 pKa = 11.84 QRR373 pKa = 11.84 ARR375 pKa = 3.12
Molecular weight: 41.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.702
IPC2_protein 5.55
IPC_protein 5.575
Toseland 5.97
ProMoST 5.868
Dawson 5.766
Bjellqvist 5.804
Wikipedia 5.741
Rodwell 5.741
Grimsley 6.16
Solomon 5.766
Lehninger 5.753
Nozaki 6.008
DTASelect 6.186
Thurlkill 6.148
EMBOSS 6.109
Sillero 6.097
Patrickios 4.584
IPC_peptide 5.779
IPC2_peptide 6.109
IPC2.peptide.svr19 6.031
Protein with the highest isoelectric point:
>tr|A0A2V0A0C7|A0A2V0A0C7_9VIRU ORF1+2p OS=Capsicum annuum amalgavirus 1 OX=2069320 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.58 SGTSGPRR8 pKa = 11.84 TPGSSGVKK16 pKa = 8.81 TVPLTARR23 pKa = 11.84 EE24 pKa = 4.02 KK25 pKa = 9.13 EE26 pKa = 3.64 IAMIEE31 pKa = 4.18 EE32 pKa = 3.92 IDD34 pKa = 4.04 EE35 pKa = 4.34 IGVTFIEE42 pKa = 4.81 LGVSAAFFDD51 pKa = 4.05 SRR53 pKa = 11.84 VYY55 pKa = 10.01 TKK57 pKa = 11.11 NLLLPAQYY65 pKa = 10.86 LRR67 pKa = 11.84 LLRR70 pKa = 11.84 QFKK73 pKa = 10.69 GKK75 pKa = 10.03 DD76 pKa = 3.02 AGEE79 pKa = 4.15 VEE81 pKa = 5.45 LIFSAAIAKK90 pKa = 9.32 KK91 pKa = 7.93 QVRR94 pKa = 11.84 ASRR97 pKa = 11.84 GIGWNKK103 pKa = 9.39 WIQFLNWAKK112 pKa = 10.82 SPAGHH117 pKa = 7.03 KK118 pKa = 10.27 AIQEE122 pKa = 3.9 VMNIEE127 pKa = 3.92 KK128 pKa = 10.66 LEE130 pKa = 3.93 RR131 pKa = 11.84 RR132 pKa = 11.84 GGGDD136 pKa = 3.28 FTVDD140 pKa = 3.08 EE141 pKa = 4.57 VAALNLFDD149 pKa = 3.95 VQRR152 pKa = 11.84 NDD154 pKa = 3.44 WIGHH158 pKa = 4.43 QKK160 pKa = 8.49 EE161 pKa = 3.74 ARR163 pKa = 11.84 AIAEE167 pKa = 4.07 HH168 pKa = 6.99 EE169 pKa = 4.01 IAEE172 pKa = 4.28 LQRR175 pKa = 11.84 KK176 pKa = 8.79 INLRR180 pKa = 11.84 RR181 pKa = 11.84 DD182 pKa = 3.39 KK183 pKa = 10.99 LDD185 pKa = 3.37 EE186 pKa = 4.49 DD187 pKa = 3.69 LRR189 pKa = 11.84 EE190 pKa = 4.07 IADD193 pKa = 3.2 QHH195 pKa = 6.63 RR196 pKa = 11.84 PVSGYY201 pKa = 10.67 VPLTDD206 pKa = 4.4 AEE208 pKa = 4.51 LNLRR212 pKa = 11.84 CWNFFRR218 pKa = 11.84 QAHH221 pKa = 5.36 EE222 pKa = 4.28 GVRR225 pKa = 11.84 GVGVHH230 pKa = 5.48 PTMSQMKK237 pKa = 9.2 EE238 pKa = 3.76 AFDD241 pKa = 3.96 TYY243 pKa = 10.75 SVHH246 pKa = 4.62 VAKK249 pKa = 10.51 RR250 pKa = 11.84 ARR252 pKa = 11.84 VEE254 pKa = 3.86 YY255 pKa = 9.09 MRR257 pKa = 11.84 HH258 pKa = 5.65 GDD260 pKa = 4.02 HH261 pKa = 7.27 VPLLQEE267 pKa = 4.64 YY268 pKa = 9.06 INQKK272 pKa = 9.15 IAHH275 pKa = 6.71 FDD277 pKa = 3.25 AVGDD281 pKa = 3.81 KK282 pKa = 10.58 RR283 pKa = 11.84 QVRR286 pKa = 11.84 HH287 pKa = 7.0 LIFNWQEE294 pKa = 3.61 WVMKK298 pKa = 10.1 WMVKK302 pKa = 9.05 WDD304 pKa = 3.26 VQTRR308 pKa = 11.84 VKK310 pKa = 10.66 LMRR313 pKa = 11.84 DD314 pKa = 3.12 IPVGTLNPRR323 pKa = 11.84 GNQTLCSRR331 pKa = 11.84 LSEE334 pKa = 4.33 QIPMNRR340 pKa = 11.84 LLEE343 pKa = 4.11 KK344 pKa = 10.37 RR345 pKa = 11.84 VISYY349 pKa = 10.51 RR350 pKa = 11.84 PAPQLSKK357 pKa = 10.43 MLEE360 pKa = 4.19 CKK362 pKa = 10.59 AEE364 pKa = 4.04 SLALLRR370 pKa = 11.84 ANGRR374 pKa = 11.84 VEE376 pKa = 4.25 IIIMPGGCDD385 pKa = 4.09 LRR387 pKa = 11.84 AIPCARR393 pKa = 11.84 SRR395 pKa = 11.84 WEE397 pKa = 3.43 KK398 pKa = 10.62 GIRR401 pKa = 11.84 KK402 pKa = 9.41 IIGGGEE408 pKa = 3.81 MLNWKK413 pKa = 9.47 VAGNMYY419 pKa = 10.31 RR420 pKa = 11.84 GGGCYY425 pKa = 9.92 SDD427 pKa = 5.08 AIKK430 pKa = 10.69 LLSSASTIPPGRR442 pKa = 11.84 LLHH445 pKa = 6.0 QCFSLPGARR454 pKa = 11.84 AALGLPSNLSVPVGKK469 pKa = 10.62 GSCRR473 pKa = 11.84 VKK475 pKa = 10.79 NYY477 pKa = 10.18 NDD479 pKa = 3.49 EE480 pKa = 4.26 ATTGPFLYY488 pKa = 10.81 SFGIKK493 pKa = 9.6 KK494 pKa = 10.39 KK495 pKa = 10.57 YY496 pKa = 10.6 GLDD499 pKa = 3.54 NEE501 pKa = 4.47 LQEE504 pKa = 4.31 IMEE507 pKa = 4.37 NAYY510 pKa = 10.31 HH511 pKa = 6.97 HH512 pKa = 6.39 FAEE515 pKa = 5.25 CKK517 pKa = 8.57 TSSSALPFFTARR529 pKa = 11.84 VGFRR533 pKa = 11.84 SKK535 pKa = 10.69 LLPMGEE541 pKa = 4.35 ALRR544 pKa = 11.84 KK545 pKa = 9.16 FADD548 pKa = 3.68 NQPMGRR554 pKa = 11.84 CVMMMDD560 pKa = 4.39 ALEE563 pKa = 4.24 QFASSPLYY571 pKa = 10.57 NVLSKK576 pKa = 10.17 YY577 pKa = 9.55 TSDD580 pKa = 3.38 RR581 pKa = 11.84 SKK583 pKa = 11.09 GATSFRR589 pKa = 11.84 NSVVRR594 pKa = 11.84 ASSDD598 pKa = 2.51 WMYY601 pKa = 11.47 LWDD604 pKa = 3.95 EE605 pKa = 4.2 VKK607 pKa = 10.4 EE608 pKa = 4.02 ASVCVEE614 pKa = 4.19 LDD616 pKa = 2.69 WSKK619 pKa = 10.97 FDD621 pKa = 4.67 RR622 pKa = 11.84 EE623 pKa = 4.23 RR624 pKa = 11.84 PSADD628 pKa = 3.71 LLFMIDD634 pKa = 3.7 VIISCFEE641 pKa = 3.66 PSNRR645 pKa = 11.84 YY646 pKa = 6.72 EE647 pKa = 3.88 VRR649 pKa = 11.84 MLEE652 pKa = 4.14 AYY654 pKa = 8.38 GICMRR659 pKa = 11.84 RR660 pKa = 11.84 ALVEE664 pKa = 4.01 RR665 pKa = 11.84 VLITDD670 pKa = 3.72 DD671 pKa = 3.8 GGVFEE676 pKa = 5.08 IEE678 pKa = 4.09 GMVPSGSLWTGWLDD692 pKa = 3.31 TALNILYY699 pKa = 10.47 LNAALRR705 pKa = 11.84 HH706 pKa = 5.69 LDD708 pKa = 3.43 IAPSSASPKK717 pKa = 10.37 CAGDD721 pKa = 3.87 DD722 pKa = 3.72 NLTLFYY728 pKa = 10.22 RR729 pKa = 11.84 DD730 pKa = 3.92 PGDD733 pKa = 3.63 EE734 pKa = 3.89 VLLRR738 pKa = 11.84 LKK740 pKa = 10.72 VVLNEE745 pKa = 3.73 WFRR748 pKa = 11.84 AGIKK752 pKa = 10.67 DD753 pKa = 3.54 EE754 pKa = 4.96 DD755 pKa = 4.21 FVITRR760 pKa = 11.84 PPYY763 pKa = 9.99 HH764 pKa = 6.36 VRR766 pKa = 11.84 TYY768 pKa = 10.78 QAVFPSGLDD777 pKa = 3.46 LSKK780 pKa = 10.26 GTSKK784 pKa = 10.42 IIHH787 pKa = 5.75 KK788 pKa = 10.25 AYY790 pKa = 8.8 WRR792 pKa = 11.84 EE793 pKa = 3.77 FEE795 pKa = 4.72 DD796 pKa = 3.99 EE797 pKa = 4.16 VRR799 pKa = 11.84 VDD801 pKa = 3.35 MEE803 pKa = 4.48 RR804 pKa = 11.84 GLSHH808 pKa = 6.59 RR809 pKa = 11.84 WEE811 pKa = 4.18 YY812 pKa = 10.85 RR813 pKa = 11.84 FKK815 pKa = 10.97 GCPKK819 pKa = 10.18 FLSCYY824 pKa = 7.7 WLEE827 pKa = 4.37 DD828 pKa = 3.27 GRR830 pKa = 11.84 PIRR833 pKa = 11.84 PSSEE837 pKa = 3.84 NLEE840 pKa = 3.88 KK841 pKa = 11.0 LLFPEE846 pKa = 5.71 GVHH849 pKa = 6.99 SSIDD853 pKa = 3.58 DD854 pKa = 4.04 YY855 pKa = 11.26 IASVLSMVVDD865 pKa = 4.17 NPFNDD870 pKa = 3.55 HH871 pKa = 6.95 NINHH875 pKa = 6.88 LKK877 pKa = 10.03 HH878 pKa = 6.7 RR879 pKa = 11.84 YY880 pKa = 8.73 LIAMQIKK887 pKa = 9.52 RR888 pKa = 11.84 LSAAGGRR895 pKa = 11.84 CGDD898 pKa = 3.29 IMDD901 pKa = 5.3 LARR904 pKa = 11.84 IRR906 pKa = 11.84 PRR908 pKa = 11.84 HH909 pKa = 5.77 DD910 pKa = 3.26 EE911 pKa = 4.2 EE912 pKa = 5.2 VPVPQIAVWRR922 pKa = 11.84 RR923 pKa = 11.84 VKK925 pKa = 10.54 EE926 pKa = 4.0 YY927 pKa = 10.49 IDD929 pKa = 3.93 LDD931 pKa = 3.74 EE932 pKa = 4.78 YY933 pKa = 11.55 GPTKK937 pKa = 10.53 HH938 pKa = 6.83 YY939 pKa = 10.41 IDD941 pKa = 4.53 EE942 pKa = 4.73 FNSFVTGVTSLYY954 pKa = 11.07 ARR956 pKa = 11.84 ATTGGLDD963 pKa = 3.17 AYY965 pKa = 10.18 KK966 pKa = 11.12 VMDD969 pKa = 5.29 LIKK972 pKa = 10.3 GNSPIGRR979 pKa = 11.84 GQWGNDD985 pKa = 2.59 VMAWIRR991 pKa = 11.84 FVRR994 pKa = 11.84 DD995 pKa = 3.55 HH996 pKa = 6.95 PATRR1000 pKa = 11.84 YY1001 pKa = 9.62 LKK1003 pKa = 8.66 EE1004 pKa = 3.66 TRR1006 pKa = 11.84 RR1007 pKa = 11.84 YY1008 pKa = 7.92 KK1009 pKa = 11.11 DD1010 pKa = 3.28 EE1011 pKa = 4.99 MIHH1014 pKa = 7.31 IDD1016 pKa = 3.39 QQTPLRR1022 pKa = 11.84 PEE1024 pKa = 3.75 VTGALAILSNALFNYY1039 pKa = 9.52 VYY1041 pKa = 10.22 EE1042 pKa = 4.51 DD1043 pKa = 3.2 SRR1045 pKa = 11.84 EE1046 pKa = 3.93 FSLSIANRR1054 pKa = 11.84 IRR1056 pKa = 11.84 TKK1058 pKa = 10.7 RR1059 pKa = 11.84 SVNN1062 pKa = 3.33
Molecular weight: 121.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.691
IPC2_protein 7.995
IPC_protein 7.819
Toseland 7.658
ProMoST 8.404
Dawson 8.609
Bjellqvist 8.843
Wikipedia 8.595
Rodwell 8.668
Grimsley 7.717
Solomon 8.668
Lehninger 8.653
Nozaki 8.975
DTASelect 8.609
Thurlkill 8.682
EMBOSS 8.799
Sillero 8.96
Patrickios 4.584
IPC_peptide 8.653
IPC2_peptide 7.658
IPC2.peptide.svr19 7.855
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1437
375
1062
718.5
81.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.724 ± 0.541
1.253 ± 0.496
5.985 ± 0.209
7.168 ± 0.82
3.758 ± 0.146
7.655 ± 1.112
2.644 ± 0.279
5.776 ± 0.356
5.358 ± 0.147
8.699 ± 0.619
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.784 ± 0.327
3.688 ± 0.379
4.523 ± 0.005
3.41 ± 0.967
8.142 ± 0.205
5.846 ± 1.062
3.688 ± 0.291
6.959 ± 0.389
1.949 ± 0.309
2.992 ± 0.566
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here