Lutibacter sp. HS1-25
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3423 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1MWV7|A0A4V1MWV7_9FLAO Peptidase M23 OS=Lutibacter sp. HS1-25 OX=2485000 GN=EC396_00325 PE=4 SV=1
MM1 pKa = 7.44 IKK3 pKa = 10.21 SIKK6 pKa = 10.05 LAILSCFFILPVCAQTTIDD25 pKa = 3.75 SFDD28 pKa = 3.77 FEE30 pKa = 4.95 FGLDD34 pKa = 3.14 SWTQSAGDD42 pKa = 4.29 DD43 pKa = 3.62 FDD45 pKa = 3.95 LTRR48 pKa = 11.84 LSGGTPSAGTGPNIASSGNYY68 pKa = 9.92 YY69 pKa = 9.8 MFAEE73 pKa = 4.44 MSSPRR78 pKa = 11.84 LNGDD82 pKa = 3.22 VAIFTSPVIDD92 pKa = 3.66 LAGYY96 pKa = 6.92 MQPTFSFDD104 pKa = 3.19 YY105 pKa = 10.91 HH106 pKa = 6.27 MYY108 pKa = 9.96 SSNSISDD115 pKa = 3.55 VGTLALEE122 pKa = 4.13 INEE125 pKa = 4.93 NNTGWQSTSFSLTGSQNVDD144 pKa = 2.94 QNTWLSGLVDD154 pKa = 4.2 LLAYY158 pKa = 10.18 QGSLVQFRR166 pKa = 11.84 FVITRR171 pKa = 11.84 ANSWQGDD178 pKa = 3.57 IAFDD182 pKa = 4.05 NIAIEE187 pKa = 4.37 GTALVAGVEE196 pKa = 4.64 IYY198 pKa = 11.03 LSGNGISIPSGKK210 pKa = 7.99 TAFSVTDD217 pKa = 3.58 GTDD220 pKa = 3.5 FGSVNLASSSIAQTFTIEE238 pKa = 4.16 NWGLDD243 pKa = 3.38 NLVLNNSPYY252 pKa = 10.91 VVITGADD259 pKa = 3.21 AANFSVDD266 pKa = 3.69 LSSTTSPISSANSTTFQVTFSPLSLGSKK294 pKa = 8.18 TATIQISNNDD304 pKa = 3.66 SDD306 pKa = 3.78 EE307 pKa = 4.2 SLYY310 pKa = 10.63 TFNIAGFGVQNFFDD324 pKa = 4.08 SDD326 pKa = 3.42 GDD328 pKa = 4.37 GIFDD332 pKa = 4.95 NVDD335 pKa = 2.89 IDD337 pKa = 4.85 DD338 pKa = 5.23 DD339 pKa = 4.41 NDD341 pKa = 4.77 GILDD345 pKa = 4.54 AIEE348 pKa = 4.86 EE349 pKa = 4.4 NTCKK353 pKa = 10.39 NSSISIPANYY363 pKa = 9.81 KK364 pKa = 10.02 FLNEE368 pKa = 4.19 TFGTLTPGTRR378 pKa = 11.84 TTINTSYY385 pKa = 11.18 NATTTYY391 pKa = 10.17 CYY393 pKa = 10.69 EE394 pKa = 4.71 PGTGSGSSCGSTWILDD410 pKa = 3.56 DD411 pKa = 3.85 GEE413 pKa = 4.6 YY414 pKa = 11.04 VVVSKK419 pKa = 10.81 IAGTIASDD427 pKa = 3.57 PEE429 pKa = 4.56 NIHH432 pKa = 7.35 GDD434 pKa = 3.94 LAWTNVLDD442 pKa = 3.77 HH443 pKa = 6.87 TPNDD447 pKa = 3.82 GDD449 pKa = 3.75 TGGMAVFNAAWAPGVFYY466 pKa = 8.22 EE467 pKa = 4.4 TTITGILPNVPITYY481 pKa = 10.16 SFWVLNIMAQSTYY494 pKa = 10.78 SGSILPNVTVQFLDD508 pKa = 3.48 LLGNEE513 pKa = 4.18 ITSIDD518 pKa = 3.23 TGEE521 pKa = 4.41 FGRR524 pKa = 11.84 CAAGTGDD531 pKa = 3.55 NTCAEE536 pKa = 4.35 NEE538 pKa = 4.16 WQNFTRR544 pKa = 11.84 NDD546 pKa = 3.25 IVLGNVDD553 pKa = 3.38 KK554 pKa = 11.12 FIVRR558 pKa = 11.84 FINNAPGGAGNDD570 pKa = 3.6 LALDD574 pKa = 5.07 DD575 pKa = 4.62 IVISQTLCDD584 pKa = 3.73 TDD586 pKa = 3.75 SDD588 pKa = 4.29 GVADD592 pKa = 5.64 IFDD595 pKa = 5.05 LDD597 pKa = 4.12 SDD599 pKa = 3.99 NDD601 pKa = 4.68 GIPDD605 pKa = 3.57 VVEE608 pKa = 4.64 AGLGSLSAGTAKK620 pKa = 10.29 IPFPSGWVDD629 pKa = 3.55 ANANGMHH636 pKa = 7.21 DD637 pKa = 3.66 AAEE640 pKa = 4.41 GNIPLDD646 pKa = 3.52 SDD648 pKa = 4.21 GDD650 pKa = 3.94 GTPNYY655 pKa = 10.45 LDD657 pKa = 5.03 LDD659 pKa = 4.06 SDD661 pKa = 3.74 NDD663 pKa = 4.41 SIFDD667 pKa = 3.61 VDD669 pKa = 3.79 EE670 pKa = 4.8 SGAGNIANATFQNGDD685 pKa = 3.08 GDD687 pKa = 3.46 ISGNGVGQGPDD698 pKa = 2.89 TDD700 pKa = 4.1 YY701 pKa = 11.49 VRR703 pKa = 11.84 EE704 pKa = 3.97 TDD706 pKa = 3.81 YY707 pKa = 11.64 EE708 pKa = 4.38 SDD710 pKa = 3.24 GTLEE714 pKa = 4.05 YY715 pKa = 9.41 FTDD718 pKa = 5.98 GILDD722 pKa = 3.41 IYY724 pKa = 11.16 DD725 pKa = 3.77 YY726 pKa = 11.56 FNGNTFEE733 pKa = 4.09 TAYY736 pKa = 10.72 GNEE739 pKa = 3.98 NQGLGNTYY747 pKa = 10.65 YY748 pKa = 11.28 VLDD751 pKa = 4.37 SDD753 pKa = 5.12 ADD755 pKa = 4.41 GIPDD759 pKa = 3.75 YY760 pKa = 11.02 MDD762 pKa = 3.21 VTSDD766 pKa = 3.26 GVTFDD771 pKa = 4.11 ISHH774 pKa = 6.79 TLYY777 pKa = 11.37 ANLDD781 pKa = 3.77 ANNDD785 pKa = 3.56 GVIDD789 pKa = 3.99 DD790 pKa = 4.71 TNDD793 pKa = 3.3 SEE795 pKa = 5.17 GDD797 pKa = 3.93 GIVDD801 pKa = 5.49 LFDD804 pKa = 3.75 TSDD807 pKa = 3.23 ATFGSPRR814 pKa = 11.84 DD815 pKa = 3.7 LDD817 pKa = 4.11 RR818 pKa = 11.84 KK819 pKa = 9.12 LHH821 pKa = 6.43 LYY823 pKa = 10.35 FDD825 pKa = 3.98 GRR827 pKa = 11.84 NDD829 pKa = 3.51 YY830 pKa = 11.49 AEE832 pKa = 5.57 DD833 pKa = 3.46 IAVINAWGEE842 pKa = 3.82 VSMMCWVKK850 pKa = 10.35 IDD852 pKa = 3.96 PSSTGIQHH860 pKa = 7.41 ILGQNEE866 pKa = 3.83 FFIQLNADD874 pKa = 3.56 KK875 pKa = 9.96 TISAHH880 pKa = 6.97 ASGSNLNYY888 pKa = 8.69 STPLTLNQWVHH899 pKa = 5.92 IATTYY904 pKa = 10.78 SNSNNEE910 pKa = 3.74 FKK912 pKa = 11.14 LFINGQEE919 pKa = 3.99 IAVDD923 pKa = 3.53 TGISGSLSADD933 pKa = 2.98 TSLFTLGKK941 pKa = 10.15 IPGGNSNFYY950 pKa = 10.26 HH951 pKa = 7.08 GYY953 pKa = 8.77 LDD955 pKa = 3.6 EE956 pKa = 4.2 VRR958 pKa = 11.84 VFNKK962 pKa = 10.34 ALSTNEE968 pKa = 3.76 LQKK971 pKa = 10.41 IVYY974 pKa = 9.33 QEE976 pKa = 3.94 IEE978 pKa = 4.2 NNSGIVRR985 pKa = 11.84 GSIIPMDD992 pKa = 3.43 VTNYY996 pKa = 9.77 IDD998 pKa = 3.63 EE999 pKa = 4.53 ATVNPLDD1006 pKa = 3.29 WTYY1009 pKa = 10.73 LQRR1012 pKa = 11.84 YY1013 pKa = 8.32 FKK1015 pKa = 10.27 MDD1017 pKa = 3.17 VYY1019 pKa = 11.09 KK1020 pKa = 10.94 DD1021 pKa = 4.54 DD1022 pKa = 5.66 IIDD1025 pKa = 4.4 DD1026 pKa = 4.51 LSTATVDD1033 pKa = 2.75 IGTGAKK1039 pKa = 9.22 IYY1041 pKa = 10.51 NMKK1044 pKa = 10.44 IIDD1047 pKa = 3.94 VQNAPLPFVTNQSGTLPTAVNHH1069 pKa = 6.82 SINGVNGNDD1078 pKa = 3.34 VITYY1082 pKa = 8.81 DD1083 pKa = 3.27 WSIVKK1088 pKa = 9.81 ILHH1091 pKa = 7.1 DD1092 pKa = 4.36 DD1093 pKa = 3.87 VYY1095 pKa = 11.48 FNNNQKK1101 pKa = 10.33 HH1102 pKa = 5.96 LGLFVNQLDD1111 pKa = 3.83 ASLNPIEE1118 pKa = 6.2 FSIQNNSEE1126 pKa = 4.65 LNVSWYY1132 pKa = 10.84 LKK1134 pKa = 10.65 LDD1136 pKa = 3.44 GFIDD1140 pKa = 4.87 LEE1142 pKa = 4.64 GKK1144 pKa = 9.29 SQLVQGAEE1152 pKa = 4.12 SVLDD1156 pKa = 3.94 EE1157 pKa = 5.24 DD1158 pKa = 3.7 SAGYY1162 pKa = 9.83 IEE1164 pKa = 5.33 QDD1166 pKa = 3.32 QQGTQSSYY1174 pKa = 11.38 NYY1176 pKa = 10.26 NYY1178 pKa = 7.06 WTSSVSPIAAGAGTNNASNAIAEE1201 pKa = 4.29 VLLDD1205 pKa = 3.6 GTIEE1209 pKa = 4.28 SNPVSINFKK1218 pKa = 9.53 PAYY1221 pKa = 7.47 TAADD1225 pKa = 3.86 GAVTSPITLSSYY1237 pKa = 9.23 WLYY1240 pKa = 10.6 TFNGTNSDD1248 pKa = 3.39 YY1249 pKa = 11.46 DD1250 pKa = 3.41 SWVSINEE1257 pKa = 3.74 NSLLKK1262 pKa = 10.77 AGEE1265 pKa = 4.72 GYY1267 pKa = 8.83 TMKK1270 pKa = 9.84 GTSGVASITDD1280 pKa = 3.42 CQNYY1284 pKa = 8.29 VFKK1287 pKa = 10.91 GKK1289 pKa = 8.78 PHH1291 pKa = 6.57 NGDD1294 pKa = 2.56 ITLNIAINNDD1304 pKa = 3.35 RR1305 pKa = 11.84 LVGNPYY1311 pKa = 10.32 PSALDD1316 pKa = 3.44 ANAFILDD1323 pKa = 4.37 NINDD1327 pKa = 4.18 CGGNAATNIFNGALYY1342 pKa = 10.04 FWDD1345 pKa = 3.69 HH1346 pKa = 6.55 FSGKK1350 pKa = 6.89 THH1352 pKa = 6.62 ILKK1355 pKa = 10.36 EE1356 pKa = 4.22 YY1357 pKa = 8.51 VGGYY1361 pKa = 7.51 ATYY1364 pKa = 9.86 TIMGGAKK1371 pKa = 9.81 AYY1373 pKa = 10.75 ANDD1376 pKa = 3.37 EE1377 pKa = 4.47 RR1378 pKa = 11.84 INNNGSSGVKK1388 pKa = 9.86 VPEE1391 pKa = 4.03 RR1392 pKa = 11.84 YY1393 pKa = 9.38 IPANQGFFVVAAIDD1407 pKa = 3.83 TVSNSINTSVTGGTITFKK1425 pKa = 10.83 NSQRR1429 pKa = 11.84 KK1430 pKa = 9.28 CIIEE1434 pKa = 4.12 DD1435 pKa = 3.35 SGNSVFLKK1443 pKa = 9.51 STHH1446 pKa = 5.98 KK1447 pKa = 10.81 KK1448 pKa = 9.34 EE1449 pKa = 4.51 AIKK1452 pKa = 10.27 EE1453 pKa = 4.08 AIIEE1457 pKa = 3.99 EE1458 pKa = 4.11 DD1459 pKa = 3.44 TRR1461 pKa = 11.84 PKK1463 pKa = 10.23 IRR1465 pKa = 11.84 LLFKK1469 pKa = 10.93 SPTGYY1474 pKa = 10.22 EE1475 pKa = 3.86 RR1476 pKa = 11.84 QLLVGADD1483 pKa = 3.49 QNASNDD1489 pKa = 3.06 FDD1491 pKa = 4.48 LGYY1494 pKa = 10.85 DD1495 pKa = 3.51 GLLIEE1500 pKa = 6.21 DD1501 pKa = 4.02 YY1502 pKa = 9.79 TADD1505 pKa = 3.68 MFWTFSGAKK1514 pKa = 9.45 FVIQAIKK1521 pKa = 10.67 EE1522 pKa = 3.98 FDD1524 pKa = 3.41 TNQQLKK1530 pKa = 10.59 LGLVVEE1536 pKa = 4.71 ANGLTTISIDD1546 pKa = 3.39 SLEE1549 pKa = 4.37 NFDD1552 pKa = 5.11 TNTKK1556 pKa = 10.33 CYY1558 pKa = 10.21 IYY1560 pKa = 10.96 DD1561 pKa = 3.48 NLTNKK1566 pKa = 9.97 TYY1568 pKa = 11.3 DD1569 pKa = 3.44 LTGNTAQIYY1578 pKa = 10.22 LEE1580 pKa = 4.44 AGTYY1584 pKa = 10.26 LDD1586 pKa = 5.15 RR1587 pKa = 11.84 FDD1589 pKa = 6.38 LRR1591 pKa = 11.84 FKK1593 pKa = 10.67 AQNTLAIDD1601 pKa = 3.64 NAEE1604 pKa = 3.78 ILANKK1609 pKa = 9.13 IQVFVNNNSNEE1620 pKa = 3.94 LQINNNLNTDD1630 pKa = 3.13 IQSINLYY1637 pKa = 10.39 NSLGQKK1643 pKa = 7.26 MHH1645 pKa = 5.7 TWNKK1649 pKa = 9.78 NLQNTQLVFNLNIKK1663 pKa = 6.34 TTGVYY1668 pKa = 8.25 MAQVNTTEE1676 pKa = 4.18 GSFTKK1681 pKa = 10.41 KK1682 pKa = 10.71 VIIEE1686 pKa = 4.0
Molecular weight: 182.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.859
IPC_protein 3.91
Toseland 3.681
ProMoST 4.062
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.846
Rodwell 3.732
Grimsley 3.579
Solomon 3.91
Lehninger 3.859
Nozaki 4.012
DTASelect 4.291
Thurlkill 3.719
EMBOSS 3.859
Sillero 4.037
Patrickios 1.265
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.914
Protein with the highest isoelectric point:
>tr|A0A4Q1IQB9|A0A4Q1IQB9_9FLAO SusC/RagA family TonB-linked outer membrane protein OS=Lutibacter sp. HS1-25 OX=2485000 GN=EC396_02435 PE=3 SV=1
MM1 pKa = 7.54 SVRR4 pKa = 11.84 KK5 pKa = 9.68 LKK7 pKa = 10.5 PITPGQRR14 pKa = 11.84 FRR16 pKa = 11.84 VVNGFDD22 pKa = 3.92 TITTDD27 pKa = 3.73 KK28 pKa = 10.77 PEE30 pKa = 4.26 KK31 pKa = 10.61 SLLTSNKK38 pKa = 9.63 RR39 pKa = 11.84 SGGRR43 pKa = 11.84 NNKK46 pKa = 9.29 GKK48 pKa = 8.29 MTMRR52 pKa = 11.84 YY53 pKa = 9.26 IGGGHH58 pKa = 6.44 KK59 pKa = 9.77 KK60 pKa = 9.28 QYY62 pKa = 10.44 RR63 pKa = 11.84 IIDD66 pKa = 3.77 FKK68 pKa = 11.12 RR69 pKa = 11.84 NKK71 pKa = 10.0 NGIPATVKK79 pKa = 10.05 TIEE82 pKa = 4.0 YY83 pKa = 9.86 DD84 pKa = 3.47 PNRR87 pKa = 11.84 TAFIALLNYY96 pKa = 10.38 ADD98 pKa = 3.68 GEE100 pKa = 4.11 KK101 pKa = 10.25 RR102 pKa = 11.84 YY103 pKa = 10.21 IIAQNGLQVGQTVMSGEE120 pKa = 4.22 GATPDD125 pKa = 3.47 VGNTMLLSEE134 pKa = 4.99 IPLGTTISCIEE145 pKa = 3.88 LHH147 pKa = 6.66 PGQGAVMARR156 pKa = 11.84 SAGSFAQLMARR167 pKa = 11.84 DD168 pKa = 3.96 GKK170 pKa = 10.52 YY171 pKa = 9.12 ATVKK175 pKa = 10.42 LPSGEE180 pKa = 3.83 TRR182 pKa = 11.84 MILLTCSATIGVVSNSDD199 pKa = 3.21 HH200 pKa = 6.09 QLLVSGKK207 pKa = 9.49 AGRR210 pKa = 11.84 SRR212 pKa = 11.84 WLGRR216 pKa = 11.84 RR217 pKa = 11.84 PRR219 pKa = 11.84 TRR221 pKa = 11.84 PVVMNPVDD229 pKa = 3.47 HH230 pKa = 7.05 PMGGGEE236 pKa = 4.28 GKK238 pKa = 10.51 SSGGHH243 pKa = 4.59 PRR245 pKa = 11.84 SRR247 pKa = 11.84 NGIPAKK253 pKa = 10.41 GFKK256 pKa = 9.24 TRR258 pKa = 11.84 SKK260 pKa = 9.37 TKK262 pKa = 10.4 ASNKK266 pKa = 9.95 YY267 pKa = 9.56 IIEE270 pKa = 4.06 RR271 pKa = 11.84 RR272 pKa = 11.84 KK273 pKa = 10.1 KK274 pKa = 9.82
Molecular weight: 29.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.309
IPC2_protein 9.882
IPC_protein 10.584
Toseland 10.965
ProMoST 10.628
Dawson 11.023
Bjellqvist 10.701
Wikipedia 11.213
Rodwell 11.359
Grimsley 11.052
Solomon 11.14
Lehninger 11.125
Nozaki 10.935
DTASelect 10.701
Thurlkill 10.935
EMBOSS 11.359
Sillero 10.95
Patrickios 11.082
IPC_peptide 11.155
IPC2_peptide 9.502
IPC2.peptide.svr19 8.561
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3423
0
3423
1211269
23
2492
353.9
39.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.227 ± 0.036
0.774 ± 0.012
5.292 ± 0.031
6.473 ± 0.042
5.29 ± 0.031
6.338 ± 0.04
1.629 ± 0.019
8.462 ± 0.046
8.097 ± 0.052
9.086 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.088 ± 0.021
6.932 ± 0.047
3.319 ± 0.026
3.252 ± 0.024
2.915 ± 0.024
6.433 ± 0.034
5.89 ± 0.043
6.229 ± 0.033
1.093 ± 0.016
4.182 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here