Amino acid dipepetide frequency for Lutibacter sp. HS1-25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.135AlaAla: 4.135 ± 0.073	0.589AlaCys: 0.589 ± 0.023	3.098AlaAsp: 3.098 ± 0.057	3.852AlaGlu: 3.852 ± 0.064	3.167AlaPhe: 3.167 ± 0.047	4.073AlaGly: 4.073 ± 0.063	1.017AlaHis: 1.017 ± 0.035	5.767AlaIle: 5.767 ± 0.067	4.651AlaLys: 4.651 ± 0.062	5.71AlaLeu: 5.71 ± 0.073	1.433AlaMet: 1.433 ± 0.034	3.775AlaAsn: 3.775 ± 0.068	1.886AlaPro: 1.886 ± 0.049	2.27AlaGln: 2.27 ± 0.042	1.772AlaArg: 1.772 ± 0.044	4.165AlaSer: 4.165 ± 0.059	3.789AlaThr: 3.789 ± 0.061	4.073AlaVal: 4.073 ± 0.071	0.578AlaTrp: 0.578 ± 0.023	2.469AlaTyr: 2.469 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.449CysAla: 0.449 ± 0.018	0.122CysCys: 0.122 ± 0.009	0.458CysAsp: 0.458 ± 0.024	0.53CysGlu: 0.53 ± 0.023	0.422CysPhe: 0.422 ± 0.02	0.594CysGly: 0.594 ± 0.026	0.217CysHis: 0.217 ± 0.022	0.611CysIle: 0.611 ± 0.026	0.589CysLys: 0.589 ± 0.022	0.627CysLeu: 0.627 ± 0.023	0.139CysMet: 0.139 ± 0.01	0.501CysAsn: 0.501 ± 0.023	0.339CysPro: 0.339 ± 0.021	0.198CysGln: 0.198 ± 0.013	0.182CysArg: 0.182 ± 0.013	0.505CysSer: 0.505 ± 0.019	0.43CysThr: 0.43 ± 0.021	0.449CysVal: 0.449 ± 0.02	0.08CysTrp: 0.08 ± 0.007	0.301CysTyr: 0.301 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.566AspAla: 3.566 ± 0.055	0.419AspCys: 0.419 ± 0.021	2.654AspAsp: 2.654 ± 0.054	3.668AspGlu: 3.668 ± 0.066	3.614AspPhe: 3.614 ± 0.063	3.642AspGly: 3.642 ± 0.073	0.788AspHis: 0.788 ± 0.034	4.51AspIle: 4.51 ± 0.064	4.018AspLys: 4.018 ± 0.06	5.143AspLeu: 5.143 ± 0.069	1.004AspMet: 1.004 ± 0.03	3.185AspAsn: 3.185 ± 0.054	1.64AspPro: 1.64 ± 0.043	1.358AspGln: 1.358 ± 0.032	1.542AspArg: 1.542 ± 0.04	2.811AspSer: 2.811 ± 0.053	2.552AspThr: 2.552 ± 0.048	3.437AspVal: 3.437 ± 0.051	0.769AspTrp: 0.769 ± 0.027	2.597AspTyr: 2.597 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	4.138GluAla: 4.138 ± 0.073	0.357GluCys: 0.357 ± 0.017	3.237GluAsp: 3.237 ± 0.056	4.292GluGlu: 4.292 ± 0.077	3.077GluPhe: 3.077 ± 0.052	3.616GluGly: 3.616 ± 0.057	0.997GluHis: 0.997 ± 0.029	6.351GluIle: 6.351 ± 0.089	5.91GluLys: 5.91 ± 0.091	6.023GluLeu: 6.023 ± 0.083	1.559GluMet: 1.559 ± 0.041	5.395GluAsn: 5.395 ± 0.064	1.563GluPro: 1.563 ± 0.041	2.212GluGln: 2.212 ± 0.049	2.053GluArg: 2.053 ± 0.042	3.226GluSer: 3.226 ± 0.054	3.634GluThr: 3.634 ± 0.058	4.181GluVal: 4.181 ± 0.056	0.617GluTrp: 0.617 ± 0.023	2.289GluTyr: 2.289 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	2.819PheAla: 2.819 ± 0.052	0.434PheCys: 0.434 ± 0.021	3.28PheAsp: 3.28 ± 0.06	3.742PheGlu: 3.742 ± 0.06	2.636PhePhe: 2.636 ± 0.058	3.625PheGly: 3.625 ± 0.062	0.758PheHis: 0.758 ± 0.028	4.259PheIle: 4.259 ± 0.07	4.15PheLys: 4.15 ± 0.06	4.663PheLeu: 4.663 ± 0.081	1.09PheMet: 1.09 ± 0.032	3.789PheAsn: 3.789 ± 0.07	1.681PhePro: 1.681 ± 0.039	1.515PheGln: 1.515 ± 0.039	1.446PheArg: 1.446 ± 0.042	3.978PheSer: 3.978 ± 0.062	3.022PheThr: 3.022 ± 0.05	3.214PheVal: 3.214 ± 0.054	0.608PheTrp: 0.608 ± 0.02	2.193PheTyr: 2.193 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.167GlyAla: 4.167 ± 0.072	0.641GlyCys: 0.641 ± 0.026	3.259GlyAsp: 3.259 ± 0.056	3.651GlyGlu: 3.651 ± 0.059	3.68GlyPhe: 3.68 ± 0.053	4.454GlyGly: 4.454 ± 0.086	0.968GlyHis: 0.968 ± 0.032	5.653GlyIle: 5.653 ± 0.075	4.934GlyLys: 4.934 ± 0.069	5.559GlyLeu: 5.559 ± 0.076	1.527GlyMet: 1.527 ± 0.041	3.834GlyAsn: 3.834 ± 0.067	1.285GlyPro: 1.285 ± 0.038	1.693GlyGln: 1.693 ± 0.044	1.855GlyArg: 1.855 ± 0.043	3.94GlySer: 3.94 ± 0.069	3.994GlyThr: 3.994 ± 0.072	4.549GlyVal: 4.549 ± 0.069	0.797GlyTrp: 0.797 ± 0.029	2.937GlyTyr: 2.937 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	0.813HisAla: 0.813 ± 0.028	0.161HisCys: 0.161 ± 0.012	0.7HisAsp: 0.7 ± 0.031	0.799HisGlu: 0.799 ± 0.024	1.112HisPhe: 1.112 ± 0.027	0.969HisGly: 0.969 ± 0.032	0.421HisHis: 0.421 ± 0.022	1.366HisIle: 1.366 ± 0.037	1.262HisLys: 1.262 ± 0.037	1.654HisLeu: 1.654 ± 0.039	0.314HisMet: 0.314 ± 0.017	1.002HisAsn: 1.002 ± 0.03	0.857HisPro: 0.857 ± 0.037	0.66HisGln: 0.66 ± 0.022	0.543HisArg: 0.543 ± 0.021	1.034HisSer: 1.034 ± 0.036	0.854HisThr: 0.854 ± 0.029	0.79HisVal: 0.79 ± 0.026	0.224HisTrp: 0.224 ± 0.014	0.755HisTyr: 0.755 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.933IleAla: 5.933 ± 0.076	0.656IleCys: 0.656 ± 0.025	5.11IleAsp: 5.11 ± 0.07	6.256IleGlu: 6.256 ± 0.082	3.816IlePhe: 3.816 ± 0.07	5.195IleGly: 5.195 ± 0.08	1.394IleHis: 1.394 ± 0.038	6.519IleIle: 6.519 ± 0.096	6.484IleLys: 6.484 ± 0.078	7.33IleLeu: 7.33 ± 0.096	1.406IleMet: 1.406 ± 0.041	5.394IleAsn: 5.394 ± 0.071	3.308IlePro: 3.308 ± 0.056	2.707IleGln: 2.707 ± 0.048	2.359IleArg: 2.359 ± 0.043	6.337IleSer: 6.337 ± 0.079	5.156IleThr: 5.156 ± 0.077	5.314IleVal: 5.314 ± 0.073	0.733IleTrp: 0.733 ± 0.026	3.211IleTyr: 3.211 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	4.693LysAla: 4.693 ± 0.072	0.409LysCys: 0.409 ± 0.022	4.247LysAsp: 4.247 ± 0.06	5.955LysGlu: 5.955 ± 0.087	3.184LysPhe: 3.184 ± 0.05	4.676LysGly: 4.676 ± 0.066	1.339LysHis: 1.339 ± 0.038	7.225LysIle: 7.225 ± 0.088	7.033LysLys: 7.033 ± 0.094	6.964LysLeu: 6.964 ± 0.082	2.047LysMet: 2.047 ± 0.042	6.48LysAsn: 6.48 ± 0.079	2.524LysPro: 2.524 ± 0.044	2.835LysGln: 2.835 ± 0.049	2.505LysArg: 2.505 ± 0.049	4.804LysSer: 4.804 ± 0.072	5.008LysThr: 5.008 ± 0.067	4.785LysVal: 4.785 ± 0.066	0.831LysTrp: 0.831 ± 0.026	3.425LysTyr: 3.425 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	5.655LeuAla: 5.655 ± 0.066	0.646LeuCys: 0.646 ± 0.027	4.776LeuAsp: 4.776 ± 0.068	5.653LeuGlu: 5.653 ± 0.07	4.895LeuPhe: 4.895 ± 0.077	5.61LeuGly: 5.61 ± 0.078	1.318LeuHis: 1.318 ± 0.035	7.489LeuIle: 7.489 ± 0.102	8.33LeuLys: 8.33 ± 0.098	8.392LeuLeu: 8.392 ± 0.117	1.896LeuMet: 1.896 ± 0.045	6.606LeuAsn: 6.606 ± 0.088	3.335LeuPro: 3.335 ± 0.052	3.026LeuGln: 3.026 ± 0.059	2.634LeuArg: 2.634 ± 0.051	6.094LeuSer: 6.094 ± 0.079	5.299LeuThr: 5.299 ± 0.069	5.245LeuVal: 5.245 ± 0.067	0.78LeuTrp: 0.78 ± 0.032	3.179LeuTyr: 3.179 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	1.522MetAla: 1.522 ± 0.038	0.15MetCys: 0.15 ± 0.011	1.076MetAsp: 1.076 ± 0.029	1.186MetGlu: 1.186 ± 0.033	0.864MetPhe: 0.864 ± 0.031	1.377MetGly: 1.377 ± 0.036	0.405MetHis: 0.405 ± 0.018	1.459MetIle: 1.459 ± 0.037	2.101MetLys: 2.101 ± 0.041	1.916MetLeu: 1.916 ± 0.044	0.522MetMet: 0.522 ± 0.022	1.307MetAsn: 1.307 ± 0.033	0.835MetPro: 0.835 ± 0.027	0.748MetGln: 0.748 ± 0.027	0.78MetArg: 0.78 ± 0.027	1.351MetSer: 1.351 ± 0.036	1.032MetThr: 1.032 ± 0.031	1.323MetVal: 1.323 ± 0.038	0.178MetTrp: 0.178 ± 0.013	0.75MetTyr: 0.75 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	4.14AsnAla: 4.14 ± 0.056	0.61AsnCys: 0.61 ± 0.024	3.448AsnAsp: 3.448 ± 0.055	4.348AsnGlu: 4.348 ± 0.065	3.587AsnPhe: 3.587 ± 0.073	4.471AsnGly: 4.471 ± 0.079	1.062AsnHis: 1.062 ± 0.03	5.571AsnIle: 5.571 ± 0.074	5.175AsnLys: 5.175 ± 0.076	6.199AsnLeu: 6.199 ± 0.079	1.349AsnMet: 1.349 ± 0.035	5.244AsnAsn: 5.244 ± 0.09	2.904AsnPro: 2.904 ± 0.051	2.374AsnGln: 2.374 ± 0.047	1.942AsnArg: 1.942 ± 0.043	4.471AsnSer: 4.471 ± 0.074	4.164AsnThr: 4.164 ± 0.075	3.86AsnVal: 3.86 ± 0.073	0.872AsnTrp: 0.872 ± 0.032	3.529AsnTyr: 3.529 ± 0.063	0.0AsnXaa: 0.0 ± 0.0
Pro	1.865ProAla: 1.865 ± 0.048	0.193ProCys: 0.193 ± 0.013	1.747ProAsp: 1.747 ± 0.045	2.509ProGlu: 2.509 ± 0.05	1.891ProPhe: 1.891 ± 0.045	1.819ProGly: 1.819 ± 0.036	0.589ProHis: 0.589 ± 0.022	2.771ProIle: 2.771 ± 0.052	2.591ProLys: 2.591 ± 0.048	2.89ProLeu: 2.89 ± 0.054	0.663ProMet: 0.663 ± 0.024	2.44ProAsn: 2.44 ± 0.049	0.815ProPro: 0.815 ± 0.027	1.025ProGln: 1.025 ± 0.03	0.831ProArg: 0.831 ± 0.028	2.24ProSer: 2.24 ± 0.04	2.186ProThr: 2.186 ± 0.048	2.293ProVal: 2.293 ± 0.047	0.409ProTrp: 0.409 ± 0.018	1.42ProTyr: 1.42 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	1.825GlnAla: 1.825 ± 0.043	0.221GlnCys: 0.221 ± 0.017	1.405GlnAsp: 1.405 ± 0.035	2.0GlnGlu: 2.0 ± 0.05	1.725GlnPhe: 1.725 ± 0.042	1.713GlnGly: 1.713 ± 0.039	0.601GlnHis: 0.601 ± 0.02	2.737GlnIle: 2.737 ± 0.05	2.899GlnLys: 2.899 ± 0.056	3.508GlnLeu: 3.508 ± 0.057	0.76GlnMet: 0.76 ± 0.028	2.408GlnAsn: 2.408 ± 0.048	1.003GlnPro: 1.003 ± 0.03	1.555GlnGln: 1.555 ± 0.043	1.013GlnArg: 1.013 ± 0.029	1.804GlnSer: 1.804 ± 0.039	1.843GlnThr: 1.843 ± 0.047	1.84GlnVal: 1.84 ± 0.039	0.328GlnTrp: 0.328 ± 0.018	1.338GlnTyr: 1.338 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	1.829ArgAla: 1.829 ± 0.039	0.186ArgCys: 0.186 ± 0.011	1.526ArgAsp: 1.526 ± 0.04	1.782ArgGlu: 1.782 ± 0.045	1.67ArgPhe: 1.67 ± 0.034	1.699ArgGly: 1.699 ± 0.039	0.502ArgHis: 0.502 ± 0.023	2.559ArgIle: 2.559 ± 0.049	2.448ArgLys: 2.448 ± 0.052	2.761ArgLeu: 2.761 ± 0.055	0.743ArgMet: 0.743 ± 0.024	1.924ArgAsn: 1.924 ± 0.038	0.992ArgPro: 0.992 ± 0.034	0.84ArgGln: 0.84 ± 0.029	0.962ArgArg: 0.962 ± 0.029	1.626ArgSer: 1.626 ± 0.04	1.607ArgThr: 1.607 ± 0.039	1.844ArgVal: 1.844 ± 0.038	0.366ArgTrp: 0.366 ± 0.017	1.284ArgTyr: 1.284 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.706SerAla: 3.706 ± 0.056	0.606SerCys: 0.606 ± 0.026	3.354SerAsp: 3.354 ± 0.057	4.108SerGlu: 4.108 ± 0.062	3.731SerPhe: 3.731 ± 0.051	4.387SerGly: 4.387 ± 0.077	1.041SerHis: 1.041 ± 0.036	5.413SerIle: 5.413 ± 0.068	5.363SerLys: 5.363 ± 0.061	5.654SerLeu: 5.654 ± 0.076	1.226SerMet: 1.226 ± 0.029	4.385SerAsn: 4.385 ± 0.082	2.033SerPro: 2.033 ± 0.045	2.014SerGln: 2.014 ± 0.043	1.798SerArg: 1.798 ± 0.039	4.177SerSer: 4.177 ± 0.081	3.884SerThr: 3.884 ± 0.061	3.944SerVal: 3.944 ± 0.057	0.768SerTrp: 0.768 ± 0.024	2.74SerTyr: 2.74 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	3.883ThrAla: 3.883 ± 0.057	0.403ThrCys: 0.403 ± 0.02	3.061ThrAsp: 3.061 ± 0.058	3.214ThrGlu: 3.214 ± 0.052	3.166ThrPhe: 3.166 ± 0.048	4.119ThrGly: 4.119 ± 0.074	0.961ThrHis: 0.961 ± 0.032	5.538ThrIle: 5.538 ± 0.074	4.171ThrLys: 4.171 ± 0.064	5.053ThrLeu: 5.053 ± 0.065	0.979ThrMet: 0.979 ± 0.029	3.898ThrAsn: 3.898 ± 0.065	2.377ThrPro: 2.377 ± 0.04	1.881ThrGln: 1.881 ± 0.036	1.44ThrArg: 1.44 ± 0.039	4.049ThrSer: 4.049 ± 0.068	3.699ThrThr: 3.699 ± 0.07	3.917ThrVal: 3.917 ± 0.062	0.604ThrTrp: 0.604 ± 0.025	2.486ThrTyr: 2.486 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	4.035ValAla: 4.035 ± 0.077	0.518ValCys: 0.518 ± 0.021	3.604ValAsp: 3.604 ± 0.056	4.229ValGlu: 4.229 ± 0.064	3.478ValPhe: 3.478 ± 0.059	3.887ValGly: 3.887 ± 0.067	0.885ValHis: 0.885 ± 0.026	5.092ValIle: 5.092 ± 0.07	4.743ValLys: 4.743 ± 0.058	5.867ValLeu: 5.867 ± 0.073	1.15ValMet: 1.15 ± 0.033	3.929ValAsn: 3.929 ± 0.06	2.035ValPro: 2.035 ± 0.041	1.755ValGln: 1.755 ± 0.036	1.819ValArg: 1.819 ± 0.045	4.376ValSer: 4.376 ± 0.073	3.548ValThr: 3.548 ± 0.058	4.306ValVal: 4.306 ± 0.09	0.67ValTrp: 0.67 ± 0.025	2.364ValTyr: 2.364 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.66TrpAla: 0.66 ± 0.022	0.095TrpCys: 0.095 ± 0.009	0.665TrpAsp: 0.665 ± 0.026	0.683TrpGlu: 0.683 ± 0.026	0.574TrpPhe: 0.574 ± 0.022	0.759TrpGly: 0.759 ± 0.026	0.225TrpHis: 0.225 ± 0.013	0.767TrpIle: 0.767 ± 0.026	0.81TrpLys: 0.81 ± 0.028	0.96TrpLeu: 0.96 ± 0.03	0.306TrpMet: 0.306 ± 0.015	0.774TrpAsn: 0.774 ± 0.029	0.258TrpPro: 0.258 ± 0.015	0.386TrpGln: 0.386 ± 0.018	0.361TrpArg: 0.361 ± 0.016	0.703TrpSer: 0.703 ± 0.025	0.608TrpThr: 0.608 ± 0.028	0.66TrpVal: 0.66 ± 0.021	0.16TrpTrp: 0.16 ± 0.012	0.512TrpTyr: 0.512 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.434TyrAla: 2.434 ± 0.045	0.329TyrCys: 0.329 ± 0.017	2.215TyrAsp: 2.215 ± 0.052	2.272TyrGlu: 2.272 ± 0.045	2.615TyrPhe: 2.615 ± 0.053	2.692TyrGly: 2.692 ± 0.049	0.806TyrHis: 0.806 ± 0.026	2.959TyrIle: 2.959 ± 0.05	3.306TyrLys: 3.306 ± 0.061	3.982TyrLeu: 3.982 ± 0.071	0.77TyrMet: 0.77 ± 0.025	3.048TyrAsn: 3.048 ± 0.061	1.515TyrPro: 1.515 ± 0.035	1.472TyrGln: 1.472 ± 0.037	1.312TyrArg: 1.312 ± 0.033	2.64TyrSer: 2.64 ± 0.054	2.59TyrThr: 2.59 ± 0.048	2.269TyrVal: 2.269 ± 0.04	0.554TyrTrp: 0.554 ± 0.019	2.039TyrTyr: 2.039 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3423 proteins (1211269 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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