Phytohabitans rumicis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Phytohabitans

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10307 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6V8LJP0|A0A6V8LJP0_9ACTN Ribonuclease OS=Phytohabitans rumicis OX=1076125 GN=Prum_064770 PE=4 SV=1
MM1 pKa = 7.18SAVAVGRR8 pKa = 11.84PTGATEE14 pKa = 4.24GDD16 pKa = 3.74TMRR19 pKa = 11.84VRR21 pKa = 11.84ARR23 pKa = 11.84VGLWSAATLAAVGLAVPGGGPAHH46 pKa = 7.38AIDD49 pKa = 5.23DD50 pKa = 4.33CDD52 pKa = 3.77GHH54 pKa = 7.89IGAYY58 pKa = 9.41IEE60 pKa = 4.41GTSGDD65 pKa = 4.08DD66 pKa = 3.53VLEE69 pKa = 4.67GTSGPDD75 pKa = 3.2IIRR78 pKa = 11.84GHH80 pKa = 6.46GGDD83 pKa = 5.11DD84 pKa = 4.71LILGYY89 pKa = 10.32GGRR92 pKa = 11.84DD93 pKa = 3.58TINGGDD99 pKa = 4.54GDD101 pKa = 4.69DD102 pKa = 4.41LMIGGDD108 pKa = 4.25CADD111 pKa = 3.78VMHH114 pKa = 7.08GNQGNDD120 pKa = 4.19DD121 pKa = 3.79IAGLAGDD128 pKa = 4.59DD129 pKa = 3.68VVNGNVGDD137 pKa = 4.33DD138 pKa = 4.15LLIGGPGADD147 pKa = 3.55TVDD150 pKa = 3.81GGSGADD156 pKa = 3.4EE157 pKa = 5.14CSDD160 pKa = 3.66EE161 pKa = 4.23PTSDD165 pKa = 3.02EE166 pKa = 4.02QYY168 pKa = 10.55IVYY171 pKa = 10.26IIDD174 pKa = 3.41WVSTYY179 pKa = 11.47ACC181 pKa = 4.38

Molecular weight: 18.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6V8L7I3|A0A6V8L7I3_9ACTN Uncharacterized protein OS=Phytohabitans rumicis OX=1076125 GN=Prum_058760 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84GSTGRR7 pKa = 11.84VPTWSATNGPTVAASTTTAASPAAALAATRR37 pKa = 11.84TRR39 pKa = 11.84MAVRR43 pKa = 11.84TGAVISPGAAAVGGRR58 pKa = 11.84PVTARR63 pKa = 11.84PAAAIAANASVGHH76 pKa = 5.58TAGSALGPTTT86 pKa = 3.8

Molecular weight: 8.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10307

0

10307

3214334

39

3365

311.9

33.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.068 ± 0.035

0.81 ± 0.007

6.009 ± 0.02

4.809 ± 0.022

2.74 ± 0.015

9.235 ± 0.028

2.144 ± 0.014

3.405 ± 0.018

1.699 ± 0.017

10.242 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.679 ± 0.01

1.924 ± 0.019

6.178 ± 0.02

2.812 ± 0.013

8.253 ± 0.033

4.957 ± 0.019

6.248 ± 0.032

8.86 ± 0.026

1.685 ± 0.009

2.243 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski