Enterococcus sulfureus ATCC 49903
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2176 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S0P0H6|S0P0H6_9ENTE Glucose-1-phosphate adenylyltransferase GlgD subunit OS=Enterococcus sulfureus ATCC 49903 OX=1140003 GN=I573_00119 PE=4 SV=1
MM1 pKa = 7.78 AEE3 pKa = 4.06 HH4 pKa = 5.93 THH6 pKa = 6.26 DD7 pKa = 4.18 HH8 pKa = 5.82 NHH10 pKa = 6.13 DD11 pKa = 3.93 HH12 pKa = 6.86 DD13 pKa = 5.05 HH14 pKa = 7.0 EE15 pKa = 4.46 GHH17 pKa = 5.2 EE18 pKa = 5.19 HH19 pKa = 5.75 ITLVDD24 pKa = 3.5 NEE26 pKa = 4.5 GNEE29 pKa = 4.1 TLYY32 pKa = 10.99 EE33 pKa = 4.03 ILLTIDD39 pKa = 3.3 GQEE42 pKa = 3.92 EE43 pKa = 4.06 FGRR46 pKa = 11.84 NYY48 pKa = 10.54 VLLYY52 pKa = 9.37 PAGVPEE58 pKa = 4.94 DD59 pKa = 3.99 EE60 pKa = 5.07 DD61 pKa = 4.53 VEE63 pKa = 4.41 LLAYY67 pKa = 10.17 AYY69 pKa = 10.29 VEE71 pKa = 4.7 KK72 pKa = 10.43 EE73 pKa = 4.01 DD74 pKa = 4.07 GMEE77 pKa = 4.51 GDD79 pKa = 4.48 LEE81 pKa = 4.23 QIEE84 pKa = 4.77 TEE86 pKa = 4.57 KK87 pKa = 10.41 EE88 pKa = 3.02 WDD90 pKa = 3.5 MIEE93 pKa = 4.35 EE94 pKa = 4.2 VFNTFMAEE102 pKa = 4.02 EE103 pKa = 3.96 EE104 pKa = 4.43 AEE106 pKa = 4.09
Molecular weight: 12.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.777
IPC2_protein 3.973
IPC_protein 3.91
Toseland 3.745
ProMoST 3.999
Dawson 3.859
Bjellqvist 4.05
Wikipedia 3.719
Rodwell 3.745
Grimsley 3.656
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.088
Thurlkill 3.757
EMBOSS 3.745
Sillero 4.024
Patrickios 1.062
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.926
Protein with the highest isoelectric point:
>tr|S0P1D5|S0P1D5_9ENTE ABC transmembrane type-1 domain-containing protein OS=Enterococcus sulfureus ATCC 49903 OX=1140003 GN=I573_00689 PE=3 SV=1
MM1 pKa = 7.92 PKK3 pKa = 10.15 QKK5 pKa = 7.56 THH7 pKa = 6.75 RR8 pKa = 11.84 GLAKK12 pKa = 9.82 RR13 pKa = 11.84 VKK15 pKa = 8.84 RR16 pKa = 11.84 TGNGGLKK23 pKa = 10.07 RR24 pKa = 11.84 FRR26 pKa = 11.84 AFTSHH31 pKa = 6.94 RR32 pKa = 11.84 FHH34 pKa = 7.48 GKK36 pKa = 6.37 TKK38 pKa = 9.57 KK39 pKa = 9.43 QRR41 pKa = 11.84 RR42 pKa = 11.84 QLRR45 pKa = 11.84 KK46 pKa = 9.68 ASMVSKK52 pKa = 10.82 GDD54 pKa = 3.43 YY55 pKa = 10.18 KK56 pKa = 10.9 RR57 pKa = 11.84 IRR59 pKa = 11.84 QQLARR64 pKa = 11.84 MKK66 pKa = 10.67
Molecular weight: 7.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.363
IPC2_protein 10.935
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.062
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.009
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2176
0
2176
687799
29
3284
316.1
35.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.519 ± 0.053
0.606 ± 0.015
5.164 ± 0.043
7.041 ± 0.06
4.634 ± 0.051
6.332 ± 0.05
2.056 ± 0.027
7.447 ± 0.053
6.283 ± 0.048
9.976 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.574 ± 0.027
4.039 ± 0.042
3.553 ± 0.036
4.757 ± 0.048
3.786 ± 0.046
5.954 ± 0.162
6.678 ± 0.085
6.963 ± 0.043
0.929 ± 0.02
3.71 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here