Ohtaekwangia koreensis
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5828 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T5J050|A0A1T5J050_9BACT Small ribosomal subunit biogenesis GTPase RsgA OS=Ohtaekwangia koreensis OX=688867 GN=rsgA PE=3 SV=1
MM1 pKa = 7.67 KK2 pKa = 10.72 NNLLLSGAFCALTFFASCSDD22 pKa = 3.71 DD23 pKa = 4.87 DD24 pKa = 4.47 IPAIEE29 pKa = 4.27 YY30 pKa = 10.4 KK31 pKa = 10.56 EE32 pKa = 3.86 QGFVKK37 pKa = 10.6 GQITGTASDD46 pKa = 4.45 DD47 pKa = 3.25 ATAINEE53 pKa = 4.09 SFSYY57 pKa = 10.52 SKK59 pKa = 11.09 YY60 pKa = 9.68 NPGYY64 pKa = 10.09 FDD66 pKa = 4.76 GPYY69 pKa = 10.26 ASNYY73 pKa = 8.43 VVNDD77 pKa = 3.2 NGTIDD82 pKa = 3.61 INIVRR87 pKa = 11.84 QDD89 pKa = 3.41 VQGGGGVYY97 pKa = 10.2 INLLLDD103 pKa = 4.08 GPGDD107 pKa = 3.52 TSPAYY112 pKa = 9.49 EE113 pKa = 4.54 VEE115 pKa = 3.73 LTYY118 pKa = 10.57 IKK120 pKa = 10.66 EE121 pKa = 4.08 SDD123 pKa = 3.55 KK124 pKa = 10.88 ILYY127 pKa = 10.03 FSTEE131 pKa = 3.72 SDD133 pKa = 3.28 SDD135 pKa = 3.98 NEE137 pKa = 4.15 SDD139 pKa = 3.63 VTNFSFDD146 pKa = 3.32 ATSGRR151 pKa = 11.84 VKK153 pKa = 10.99 GEE155 pKa = 3.46 FSIEE159 pKa = 3.93 GSDD162 pKa = 3.81 NSTNNDD168 pKa = 2.86 ATVTGSFDD176 pKa = 3.56 VVVKK180 pKa = 8.96 QVIQQ184 pKa = 3.35
Molecular weight: 19.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.579
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.126
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.884
Patrickios 1.036
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.771
Protein with the highest isoelectric point:
>tr|A0A1T5LHQ2|A0A1T5LHQ2_9BACT Lipid A Biosynthesis N-terminal domain-containing protein OS=Ohtaekwangia koreensis OX=688867 GN=SAMN05660236_3259 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.41 KK6 pKa = 9.45 RR7 pKa = 11.84 KK8 pKa = 6.94 KK9 pKa = 10.11 HH10 pKa = 5.69 KK11 pKa = 9.8 MATHH15 pKa = 6.02 KK16 pKa = 10.33 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.26 NRR25 pKa = 11.84 HH26 pKa = 4.71 KK27 pKa = 10.87 KK28 pKa = 9.36 KK29 pKa = 10.77
Molecular weight: 3.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.41
IPC2_protein 10.965
IPC_protein 12.442
Toseland 12.647
ProMoST 13.1
Dawson 12.647
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.705
Grimsley 12.691
Solomon 13.115
Lehninger 13.027
Nozaki 12.647
DTASelect 12.618
Thurlkill 12.647
EMBOSS 13.13
Sillero 12.647
Patrickios 12.427
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.937
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5828
0
5828
2158248
25
3719
370.3
41.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.141 ± 0.032
0.755 ± 0.009
5.428 ± 0.023
5.968 ± 0.037
4.886 ± 0.024
6.698 ± 0.034
1.894 ± 0.015
7.354 ± 0.03
6.763 ± 0.04
9.256 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.167 ± 0.015
5.276 ± 0.029
3.719 ± 0.018
3.685 ± 0.018
4.169 ± 0.022
6.784 ± 0.03
6.134 ± 0.049
6.537 ± 0.024
1.244 ± 0.012
4.143 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here