Aspergillus homomorphus (strain CBS 101889)
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11360 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A395HQW8|A0A395HQW8_ASPHC Co-chaperone Hsc20 OS=Aspergillus homomorphus (strain CBS 101889) OX=1450537 GN=BO97DRAFT_407786 PE=3 SV=1
MM1 pKa = 7.83 ADD3 pKa = 3.95 DD4 pKa = 4.82 ALSIYY9 pKa = 10.64 DD10 pKa = 4.0 EE11 pKa = 4.57 IEE13 pKa = 4.35 IEE15 pKa = 6.07 DD16 pKa = 3.67 MTFDD20 pKa = 4.29 PNLQIYY26 pKa = 9.03 HH27 pKa = 5.7 YY28 pKa = 8.84 PCPCGDD34 pKa = 3.52 RR35 pKa = 11.84 FEE37 pKa = 4.77 IAIDD41 pKa = 3.76 DD42 pKa = 4.15 LRR44 pKa = 11.84 DD45 pKa = 3.75 GEE47 pKa = 5.2 DD48 pKa = 2.94 IAVCPSCSLMIRR60 pKa = 11.84 VIFDD64 pKa = 3.07 EE65 pKa = 4.9 GDD67 pKa = 3.3 LVKK70 pKa = 10.93 DD71 pKa = 3.58 DD72 pKa = 5.2 DD73 pKa = 4.84 KK74 pKa = 11.72 NGSAGAVAVQAA85 pKa = 4.56
Molecular weight: 9.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.617
IPC_protein 3.617
Toseland 3.389
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.605
Rodwell 3.439
Grimsley 3.3
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 4.012
Thurlkill 3.465
EMBOSS 3.605
Sillero 3.745
Patrickios 0.846
IPC_peptide 3.592
IPC2_peptide 3.706
IPC2.peptide.svr19 3.697
Protein with the highest isoelectric point:
>tr|A0A395HQA1|A0A395HQA1_ASPHC Lumazine-binding protein OS=Aspergillus homomorphus (strain CBS 101889) OX=1450537 GN=BO97DRAFT_373533 PE=4 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.54 LAKK15 pKa = 9.74 AQRR18 pKa = 11.84 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.14 WRR45 pKa = 11.84 KK46 pKa = 7.41 TRR48 pKa = 11.84 LGII51 pKa = 4.46
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11360
0
11360
5329795
50
7149
469.2
51.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.776 ± 0.022
1.314 ± 0.01
5.514 ± 0.017
6.074 ± 0.026
3.706 ± 0.013
6.753 ± 0.021
2.524 ± 0.009
4.813 ± 0.014
4.307 ± 0.018
9.257 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.124 ± 0.008
3.468 ± 0.01
6.089 ± 0.024
4.086 ± 0.019
6.298 ± 0.019
8.29 ± 0.027
6.065 ± 0.015
6.228 ± 0.016
1.481 ± 0.008
2.832 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here