Streptomyces sp. BK161

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V3EKB4|A0A4V3EKB4_9ACTN Putative membrane protein YphA (DoxX/SURF4 family) OS=Streptomyces sp. BK161 OX=887463 GN=EV573_105583 PE=4 SV=1
MM1 pKa = 7.81TDD3 pKa = 3.42DD4 pKa = 3.32QHH6 pKa = 9.59GEE8 pKa = 4.05GTPQDD13 pKa = 3.66GGRR16 pKa = 11.84WDD18 pKa = 4.56PLPQGDD24 pKa = 4.33YY25 pKa = 11.28DD26 pKa = 4.37DD27 pKa = 4.9GATAFVQLPEE37 pKa = 4.38GGIDD41 pKa = 3.5ALLSGDD47 pKa = 4.46SPLAAPGHH55 pKa = 6.43GYY57 pKa = 10.04VPPQITATPPAHH69 pKa = 7.47DD70 pKa = 4.26GTDD73 pKa = 3.5PAAGAWGAPAGGTGWPDD90 pKa = 3.52PNAVPAQEE98 pKa = 4.6PGAGDD103 pKa = 3.56RR104 pKa = 11.84FTYY107 pKa = 10.44HH108 pKa = 7.21PGATGQWNFGDD119 pKa = 3.92ANGPEE124 pKa = 4.39PAPGPGHH131 pKa = 7.3DD132 pKa = 4.16VTGQWSIPVAGGDD145 pKa = 3.84LPDD148 pKa = 3.73EE149 pKa = 4.58SGEE152 pKa = 4.18YY153 pKa = 8.72STSALLEE160 pKa = 4.13QWGGTPPATLPGGAPAPWATGPGGAGGEE188 pKa = 4.27PWGQTAPSADD198 pKa = 4.65PYY200 pKa = 10.53AQPPGPYY207 pKa = 9.88DD208 pKa = 3.2SGAPGLPGGDD218 pKa = 3.0AHH220 pKa = 7.24GGPSPAAGAAEE231 pKa = 4.1AEE233 pKa = 4.67RR234 pKa = 11.84YY235 pKa = 9.79ASAADD240 pKa = 3.47AALDD244 pKa = 3.93EE245 pKa = 4.44AHH247 pKa = 6.34TAAEE251 pKa = 4.31ASSTAAVYY259 pKa = 7.47EE260 pKa = 4.41TFTGSPALPGATDD273 pKa = 3.16AHH275 pKa = 6.76AGHH278 pKa = 6.77GPGYY282 pKa = 10.35GRR284 pKa = 11.84QSEE287 pKa = 4.3QTFGQGVEE295 pKa = 4.51FTFGEE300 pKa = 4.53SGAEE304 pKa = 3.81QPPRR308 pKa = 11.84ASGAGTAYY316 pKa = 8.9DD317 pKa = 3.88TAPGSAYY324 pKa = 10.19DD325 pKa = 4.04SPSGLPEE332 pKa = 3.97EE333 pKa = 4.85ASSGFPDD340 pKa = 3.88APTDD344 pKa = 3.88GFPDD348 pKa = 4.05TPASSTEE355 pKa = 3.75QTEE358 pKa = 4.35AQQEE362 pKa = 4.54EE363 pKa = 5.19PGTPHH368 pKa = 6.85GSVSGVADD376 pKa = 4.02ATPSATDD383 pKa = 3.72GSTSGPEE390 pKa = 3.65GDD392 pKa = 4.81APPGFPGEE400 pKa = 4.31SALGAEE406 pKa = 5.06GPEE409 pKa = 4.03EE410 pKa = 4.24GAPSGAADD418 pKa = 4.32GSDD421 pKa = 3.28SGADD425 pKa = 3.22AAVTADD431 pKa = 4.13FPDD434 pKa = 3.67GRR436 pKa = 11.84APGDD440 pKa = 3.38GDD442 pKa = 3.77GTPEE446 pKa = 4.27GLPDD450 pKa = 4.11GAASDD455 pKa = 4.47ASPPLPPEE463 pKa = 4.2RR464 pKa = 11.84PAAAEE469 pKa = 3.67DD470 pKa = 3.47TGANEE475 pKa = 4.27EE476 pKa = 4.32SPEE479 pKa = 3.99YY480 pKa = 10.03PEE482 pKa = 4.86SRR484 pKa = 11.84EE485 pKa = 4.12SQASSASPDD494 pKa = 3.64GVPDD498 pKa = 3.45ATDD501 pKa = 3.22VVDD504 pKa = 5.28ASDD507 pKa = 4.28GADD510 pKa = 3.49PAPGDD515 pKa = 3.78GADD518 pKa = 4.56PSPEE522 pKa = 4.04PAPAAVPDD530 pKa = 3.74AATPHH535 pKa = 7.06DD536 pKa = 4.77APAPPQEE543 pKa = 4.05EE544 pKa = 4.67HH545 pKa = 6.88PLASYY550 pKa = 9.16VLRR553 pKa = 11.84VNGVDD558 pKa = 4.84RR559 pKa = 11.84PVTDD563 pKa = 2.96AWIGEE568 pKa = 4.15SLLYY572 pKa = 10.18VLRR575 pKa = 11.84EE576 pKa = 3.94RR577 pKa = 11.84LGLAGAKK584 pKa = 9.72DD585 pKa = 3.75GCSQGEE591 pKa = 4.12CGACNVQVDD600 pKa = 3.94GRR602 pKa = 11.84LVASCLVPSVTAAGSEE618 pKa = 4.0VRR620 pKa = 11.84TVEE623 pKa = 3.85GLAVDD628 pKa = 4.6GRR630 pKa = 11.84PSDD633 pKa = 3.66VQRR636 pKa = 11.84ALARR640 pKa = 11.84CGAVQCGFCVPGMAMTVHH658 pKa = 7.27DD659 pKa = 5.16LLEE662 pKa = 4.78GNPAPTEE669 pKa = 4.08LEE671 pKa = 4.08TRR673 pKa = 11.84QALCGNLCRR682 pKa = 11.84CSGYY686 pKa = 10.36RR687 pKa = 11.84GVLEE691 pKa = 3.9AVKK694 pKa = 10.18EE695 pKa = 4.37VVAEE699 pKa = 4.43RR700 pKa = 11.84EE701 pKa = 3.97AHH703 pKa = 5.13TAADD707 pKa = 4.16GEE709 pKa = 4.63TDD711 pKa = 3.16TGEE714 pKa = 4.3ARR716 pKa = 11.84IPHH719 pKa = 5.39QAGPGAGGVHH729 pKa = 6.81PSAFDD734 pKa = 3.46SPGFPSANGPSGPQGHH750 pKa = 6.63TDD752 pKa = 2.52SSGPHH757 pKa = 6.08DD758 pKa = 3.55QAYY761 pKa = 9.56GQDD764 pKa = 3.54GGQAA768 pKa = 3.33

Molecular weight: 75.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R7HNJ8|A0A4R7HNJ8_9ACTN DNA-binding transcriptional LysR family regulator OS=Streptomyces sp. BK161 OX=887463 GN=EV573_11571 PE=3 SV=1
MM1 pKa = 7.55TGPPRR6 pKa = 11.84TSYY9 pKa = 10.4RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84NPGSPRR19 pKa = 11.84HH20 pKa = 5.98GPGPPPRR27 pKa = 11.84PVPRR31 pKa = 11.84RR32 pKa = 11.84APEE35 pKa = 4.2RR36 pKa = 11.84PHH38 pKa = 8.16ADD40 pKa = 3.23APPGNPRR47 pKa = 11.84PTHH50 pKa = 5.57PRR52 pKa = 11.84APRR55 pKa = 11.84ASHH58 pKa = 6.81PAPSPRR64 pKa = 11.84ASTSEE69 pKa = 3.91HH70 pKa = 5.66PTTRR74 pKa = 11.84LRR76 pKa = 11.84APPTASLRR84 pKa = 11.84APPTVPLRR92 pKa = 11.84ARR94 pKa = 11.84PATSPRR100 pKa = 11.84RR101 pKa = 11.84PPATSPRR108 pKa = 11.84PPPTAVRR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84MGRR120 pKa = 11.84PRR122 pKa = 11.84SLGSVSRR129 pKa = 11.84AVVRR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84MGRR138 pKa = 11.84PPASGPLSRR147 pKa = 11.84VVGRR151 pKa = 11.84RR152 pKa = 11.84RR153 pKa = 11.84MGRR156 pKa = 11.84PPASGLPSRR165 pKa = 11.84AVGRR169 pKa = 11.84RR170 pKa = 11.84PSRR173 pKa = 11.84HH174 pKa = 5.21SPSRR178 pKa = 11.84RR179 pKa = 11.84PRR181 pKa = 11.84SRR183 pKa = 11.84AVGRR187 pKa = 11.84RR188 pKa = 11.84RR189 pKa = 11.84MRR191 pKa = 11.84RR192 pKa = 11.84PPASGPRR199 pKa = 11.84SPAVGRR205 pKa = 11.84RR206 pKa = 11.84PTRR209 pKa = 11.84YY210 pKa = 8.74PPSPRR215 pKa = 11.84PPSRR219 pKa = 11.84VAVRR223 pKa = 11.84RR224 pKa = 11.84PMRR227 pKa = 11.84PQSPGPLSRR236 pKa = 11.84AVGRR240 pKa = 11.84RR241 pKa = 11.84RR242 pKa = 11.84MGRR245 pKa = 11.84PPAPGLLSRR254 pKa = 11.84AVGRR258 pKa = 11.84RR259 pKa = 11.84PSRR262 pKa = 11.84HH263 pKa = 5.97PPSPRR268 pKa = 11.84PPSRR272 pKa = 11.84AVGRR276 pKa = 11.84RR277 pKa = 11.84RR278 pKa = 11.84MGRR281 pKa = 11.84PPASGLPSRR290 pKa = 11.84VAVRR294 pKa = 11.84RR295 pKa = 11.84PMRR298 pKa = 11.84PQSPGPLSRR307 pKa = 11.84VVGRR311 pKa = 11.84RR312 pKa = 11.84RR313 pKa = 11.84MGRR316 pKa = 11.84PPAPGLTGRR325 pKa = 11.84AVGRR329 pKa = 11.84RR330 pKa = 11.84PMRR333 pKa = 11.84RR334 pKa = 11.84PPSPRR339 pKa = 11.84PPSRR343 pKa = 11.84AVVCRR348 pKa = 11.84PMRR351 pKa = 11.84RR352 pKa = 11.84PGPRR356 pKa = 11.84SIRR359 pKa = 11.84PTAARR364 pKa = 11.84RR365 pKa = 11.84TGVRR369 pKa = 11.84APQPRR374 pKa = 11.84VPPVIEE380 pKa = 4.65RR381 pKa = 11.84PWGHH385 pKa = 7.02PPQPLPSAPPTTPPP399 pKa = 3.56

Molecular weight: 43.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9221

0

9221

3064903

25

8389

332.4

35.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.286 ± 0.035

0.802 ± 0.009

6.043 ± 0.022

5.647 ± 0.027

2.731 ± 0.015

9.349 ± 0.025

2.337 ± 0.013

3.095 ± 0.017

2.208 ± 0.024

10.277 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.691 ± 0.01

1.859 ± 0.016

6.107 ± 0.026

2.818 ± 0.015

7.98 ± 0.033

5.177 ± 0.019

6.344 ± 0.027

8.511 ± 0.023

1.581 ± 0.011

2.159 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski